Restriction map for
pF1KB9552
(Circular) (Six-base) MAPSORT of: pF1KB9552.seq Check: 6849 from: 1 to: 4511
pF1KB9552 4511 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 500 500
Size: 4511
AccI GT'mk_AC
Cuts at: 1549 1549
Size: 4511
Acc65I G'GTAC_C
Cuts at: 1527 1527
Size: 4511
AclI AA'CG_TT
Cuts at: 4351 4351
Size: 4511
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2336 2768 3515 3783 2336
Size: 432 747 268 3064
Fragments arranged by size:
3064 747 432 268
AflIII A'CryG_T
Cuts at: 1863 2967 3944 1863
Size: 1104 977 2430
Fragments arranged by size:
2430 1104 977
AgeI A'CCGG_T
Cuts at: 2899 2899
Size: 4511
AlwNI CAG_nnn'CTG
Cuts at: 1008 1816 3383 1008
Size: 808 1567 2136
Fragments arranged by size:
2136 1567 808
ApaI G_GGCC'C
Cuts at: 206 1196 1205 1397 1448 206
Size: 990 9 192 51 3269
Fragments arranged by size:
3269 990 192 51 9
ApaLI G'TGCA_C
Cuts at: 3281 3281
Size: 4511
ApoI r'AATT_y
Cuts at: 1515 3864 4120 1515
Size: 2349 256 1906
Fragments arranged by size:
2349 1906 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4511
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4511
AvaI C'yCGr_G
Cuts at: 180 243 790 1350 1531 180
Size: 63 547 560 181 3160
Fragments arranged by size:
3160 560 547 181 63
BamHI G'GATC_C
Cuts at: 1536 1536
Size: 4511
BanI G'GyrC_C
Cuts at: 369 394 752 1022 1527 1941 2189 2224 369
Size: 25 358 270 505 414 248 35 2656
Fragments arranged by size:
2656 505 414 358 270 248 35 25
BanII G_rGCy'C
Cuts at: 148 157 167 206 453 509 769 1196 1205
Size: 9 10 39 247 56 260 427 9
Cuts at: 1205 1397 1448 1525 2555 148
Size: 192 51 77 1030 2104
Fragments arranged by size:
2104 1030 427 260 247 192 77 56
51 39 10 9 9
BbeI G_GCGC'C
Cuts at: 398 1945 2193 398
Size: 1547 248 2716
Fragments arranged by size:
2716 1547 248
BbsI GAAGACnn'nnnn_
Cuts at: 850 1183 850
Size: 333 4178
BbvCI CC'TCA_GC
Cuts at: 1255 1255
Size: 4511
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 453 487 453
Size: 34 4477
BciVI GTATCCnnnnn_n'
Cuts at: 2409 3170 4511 2409
Size: 761 1341 2409
Fragments arranged by size:
2409 1341 761
BclI T'GATC_A
Cuts at: 2031 2031
Size: 4511
BglI GCCn_nnn'nGGC
Cuts at: 152 636 718 1020 1200 1352 152
Size: 484 82 302 180 152 3311
Fragments arranged by size:
3311 484 302 180 152 82
BglII A'GATC_T
Cuts at: 2026 4063 2026
Size: 2037 2474
BlpI GC'TnA_GC
Cuts at: 142 346 503 508 578 1275 1623 142
Size: 204 157 5 70 697 348 3030
Fragments arranged by size:
3030 697 348 204 157 70 5
Bme1580I G_kGCm'C
Cuts at: 206 374 679 1025 1077 1196 1205 1397 1448
Size: 168 305 346 52 119 9 192 51
Cuts at: 1448 2136 2229 3285 206
Size: 688 93 1056 1432
Fragments arranged by size:
1432 1056 688 346 305 192 168 119
93 52 51 9
BmgBI CAC'GTC
Cuts at: 417 417
Size: 4511
BmrI ACTGGGnnnn_n'
Cuts at: 1296 1443 1479 1723 1804 2139 4283 1296
Size: 147 36 244 81 335 2144 1524
Fragments arranged by size:
2144 1524 335 244 147 81 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 816 2878 816
Size: 2062 2449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 552 552
Size: 4511
Bpu10I CC'TnA_GC
Cuts at: 452 1255 452
Size: 803 3708
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1687 2566 3058 3356 1687
Size: 879 492 298 2842
Fragments arranged by size:
2842 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 970 1146 1177 4215 970
Size: 176 31 3038 1266
Fragments arranged by size:
3038 1266 176 31
BsaAI yAC'GTr
Cuts at: 1864 1864
Size: 4511
BsaBI GATnn'nnATC
Cuts at: 6 2025 4062 6
Size: 2019 2037 455
Fragments arranged by size:
2037 2019 455
BsaHI Gr'CG_yC
Cuts at: 395 1942 2190 2892 4388 395
Size: 1547 248 702 1496 518
Fragments arranged by size:
1547 1496 702 518 248
BsaWI w'CCGG_w
Cuts at: 1700 2221 2899 3173 3320 3731 1700
Size: 521 678 274 147 411 2480
Fragments arranged by size:
2480 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 874 904 874
Size: 30 4481
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 175 586 130
Size: 45 411 4055
Fragments arranged by size:
4055 411 45
BseYI C'CCAG_C
Cuts at: 258 519 1065 1432 1936 2694 3271 3767 258
Size: 261 546 367 504 758 577 496 1002
Fragments arranged by size:
1002 758 577 546 504 496 367 261
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 478
Size: 4511
BsiEI CG_ry'CG
Cuts at: 104 2053 2099 3307 104
Size: 1949 46 1208 1308
Fragments arranged by size:
1949 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 347 509 797 1525 2303 2493 3285 347
Size: 162 288 728 778 190 792 1573
Fragments arranged by size:
1573 792 778 728 288 190 162
Bsp1286I G_dGCh'C
Cuts at: 148 157 167 206 347 374 453 509 679
Size: 9 10 39 141 27 79 56 170
Cuts at: 679 769 797 1025 1077 1196 1205 1397 1448
Size: 90 28 228 52 119 9 192 51
Cuts at: 1448 1525 2136 2229 2303 2493 2555 3285 148
Size: 77 611 93 74 190 62 730 1374
Fragments arranged by size:
1374 730 611 228 192 190 170 141
119 93 90 79 77 74 62 56
52 51 39 28 27 10 9 9
BspEI T'CCGG_A
Cuts at: 3731 3731
Size: 4511
BspHI T'CATG_A
Cuts at: 3687 3687
Size: 4511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 500 1016 1561 1775 2077 2458 500
Size: 516 545 214 302 381 2553
Fragments arranged by size:
2553 545 516 381 302 214
BsrBI CCG'CTC
Cuts at: 50 582 2803 2857 4341 4506 50
Size: 532 2221 54 1484 165 55
Fragments arranged by size:
2221 1484 532 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2423 2423
Size: 4511
BsrFI r'CCGG_y
Cuts at: 749 1054 2509 2690 2899 749
Size: 305 1455 181 209 2361
Fragments arranged by size:
2361 1455 305 209 181
BssHII G'CGCG_C
Cuts at: 188 460 188
Size: 272 4239
BssSI C'ACGA_G
Cuts at: 955 2782 3140 955
Size: 1827 358 2326
Fragments arranged by size:
2326 1827 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1888 1888
Size: 4511
BstBI TT'CG_AA
Cuts at: 2872 2872
Size: 4511
BstXI CCAn_nnnn'nTGG
Cuts at: 814 1363 814
Size: 549 3962
BstYI r'GATC_y
Cuts at: 1 123 1106 1536 2003 2026 2361 2607 3619
Size: 122 983 430 467 23 335 246 1012
Cuts at: 3619 3705 3717 4063 1
Size: 86 12 346 449
Fragments arranged by size:
1012 983 467 449 430 346 335 246
122 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 801 1329 1440 801
Size: 528 111 3872
Fragments arranged by size:
3872 528 111
BtgI C'CryG_G
Cuts at: 107 149 813 893 1082 1136 1187 107
Size: 42 664 80 189 54 51 3431
Fragments arranged by size:
3431 664 189 80 54 51 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2461 2642 2805 2461
Size: 181 163 4167
Fragments arranged by size:
4167 181 163
BtsI GCAGTG_nn'
Cuts at: 1077 1754 1077
Size: 677 3834
DraI TTT'AAA
Cuts at: 1510 1510
Size: 4511
DraIII CAC_nnn'GTG
Cuts at: 1153 1153
Size: 4511
DrdI GACnn_nn'nnGTC
Cuts at: 2217 3075 2217
Size: 858 3653
EaeI y'GGCC_r
Cuts at: 1079 1085 1344 1418 2096 2270 2661 2688 4033
Size: 6 259 74 678 174 391 27 1345
Cuts at: 4033 1079
Size: 1557
Fragments arranged by size:
1557 1345 678 391 259 174 74 27
6
EagI C'GGCC_G
Cuts at: 2096 2096
Size: 4511
EarI CTCTTCn'nnn_
Cuts at: 2534 2744 2534
Size: 210 4301
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 236 310 654 685 1089 2719 3029 3175
Size: 109 74 344 31 404 1630 310 146
Cuts at: 3175 4321 127
Size: 1146 317
Fragments arranged by size:
1630 1146 404 344 317 310 146 109
74 31
EcoICRI GAG'CTC
Cuts at: 507 1523 507
Size: 1016 3495
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 552 2336 2768 3515 3783 552
Size: 1784 432 747 268 1280
Fragments arranged by size:
1784 1280 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 202 1192 1193 1201 1202 1333 1393 1394 1444
Size: 990 1 8 1 131 60 1 50
Cuts at: 1444 1445 1650 202
Size: 1 205 3063
Fragments arranged by size:
3063 990 205 131 60 50 8 1
1 1 1
EcoRI G'AATT_C
Cuts at: 1515 1515
Size: 4511
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 579 2530 579
Size: 1951 2560
FspI TGC'GCA
Cuts at: 2292 2292
Size: 4511
HaeII r_GCGC'y
Cuts at: 398 561 737 891 1945 2193 3215 4194 398
Size: 163 176 154 1054 248 1022 979 715
Fragments arranged by size:
1054 1022 979 715 248 176 163 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 841 873 874 906 949 981 1210 1242 2353
Size: 32 1 32 43 32 229 32 1111
Cuts at: 2353 2383 2385 2415 841
Size: 30 2 30 2937
Fragments arranged by size:
2937 1111 229 43 32 32 32 32
30 30 2 1
HincII GTy'rAC
Cuts at: 390 1550 390
Size: 1160 3351
KasI G'GCGC_C
Cuts at: 394 1941 2189 394
Size: 1547 248 2716
Fragments arranged by size:
2716 1547 248
KpnI G_GTAC'C
Cuts at: 1531 1531
Size: 4511
MluI A'CGCG_T
Cuts at: 3944 3944
Size: 4511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 856 977 992 3182 3366 856
Size: 121 15 2190 184 2001
Fragments arranged by size:
2190 2001 184 121 15
MscI TGG'CCA
Cuts at: 1087 1346 2272 1087
Size: 259 926 3326
Fragments arranged by size:
3326 926 259
MslI CAynn'nnrTG
Cuts at: 379 2500 2627 379
Size: 2121 127 2263
Fragments arranged by size:
2263 2121 127
MspA1I CmG'CkG
Cuts at: 170 350 1189 1404 1622 1936 2296 3309 3554
Size: 180 839 215 218 314 360 1013 245
Cuts at: 3554 170
Size: 1127
Fragments arranged by size:
1127 1013 839 360 314 245 218 215
180
NaeI GCC'GGC
Cuts at: 1056 2692 1056
Size: 1636 2875
NarI GG'CG_CC
Cuts at: 395 1942 2190 395
Size: 1547 248 2716
Fragments arranged by size:
2716 1547 248
NcoI C'CATG_G
Cuts at: 107 813 893 107
Size: 706 80 3725
Fragments arranged by size:
3725 706 80
NgoMIV G'CCGG_C
Cuts at: 1054 2690 1054
Size: 1636 2875
NspI r_CATG'y
Cuts at: 1564 2064 2971 1564
Size: 500 907 3104
Fragments arranged by size:
3104 907 500
PasI CC'CwG_GG
Cuts at: 660 1197 660
Size: 537 3974
PciI A'CATG_T
Cuts at: 2967 2967
Size: 4511
PflMI CCAn_nnn'nTGG
Cuts at: 1469 1469
Size: 4511
PmeI GTTT'AAAC
Cuts at: 1510 1510
Size: 4511
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3650 3682 3650
Size: 32 4479
PpuMI rG'GwC_Cy
Cuts at: 1333 1333
Size: 4511
PspOMI G'GGCC_C
Cuts at: 202 1192 1201 1393 1444 202
Size: 990 9 192 51 3269
Fragments arranged by size:
3269 990 192 51 9
PstI C_TGCA'G
Cuts at: 857 1558 2243 857
Size: 701 685 3125
Fragments arranged by size:
3125 701 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4511
PvuII CAG'CTG
Cuts at: 1936 2296 1936
Size: 360 4151
RsrII CG'GwC_CG
Cuts at: 2706 2706
Size: 4511
SacI G_AGCT'C
Cuts at: 509 1525 509
Size: 1016 3495
SacII CC_GC'GG
Cuts at: 1190 1190
Size: 4511
SalI G'TCGA_C
Cuts at: 1548 1548
Size: 4511
SapI GCTCTTCn'nnn_
Cuts at: 2534 2744 2534
Size: 210 4301
SbfI CC_TGCA'GG
Cuts at: 1558 1558
Size: 4511
ScaI AGT'ACT
Cuts at: 4028 4028
Size: 4511
SexAI A'CCwGG_T
Cuts at: 1149 1149
Size: 4511
SfcI C'TryA_G
Cuts at: 33 853 1554 2239 3232 3423 33
Size: 820 701 685 993 191 1121
Fragments arranged by size:
1121 993 820 701 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1200 1352 1200
Size: 152 4359
SfoI GGC'GCC
Cuts at: 396 1943 2191 396
Size: 1547 248 2716
Fragments arranged by size:
2716 1547 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4511
SmaI CCC'GGG
Cuts at: 182 245 1533 182
Size: 63 1288 3160
Fragments arranged by size:
3160 1288 63
SmlI C'TyrA_G
Cuts at: 1666 2581 3073 3335 1666
Size: 915 492 262 2842
Fragments arranged by size:
2842 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4511
SphI G_CATG'C
Cuts at: 1564 2064 1564
Size: 500 4011
StuI AGG'CCT
Cuts at: 1360 1360
Size: 4511
StyI C'CwwG_G
Cuts at: 107 813 893 1645 1999 107
Size: 706 80 752 354 2619
Fragments arranged by size:
2619 752 706 354 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 770 837 1040 2094 2227 2686 2895 770
Size: 67 203 1054 133 459 209 2386
Fragments arranged by size:
2386 1054 459 209 203 133 67
TatI w'GTAC_w
Cuts at: 4026 4026
Size: 4511
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4028 4060 4028
Size: 32 4479
Tth111I GACn'n_nGTC
Cuts at: 1494 2308 1494
Size: 814 3697
XbaI T'CTAG_A
Cuts at: 1542 1542
Size: 4511
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2006 2006
Size: 4511
XmaI C'CCGG_G
Cuts at: 180 243 1531 180
Size: 63 1288 3160
Fragments arranged by size:
3160 1288 63
XmnI GAAnn'nnTTC
Cuts at: 436 436
Size: 4511
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BfrBI BmtI BsiWI BsmI BsmBI BsrGI
BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PshAI PsiI PspXI PsrI SanDI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI