Restriction map for
pF1KB9551
(Circular) (Six-base) MAPSORT of: pF1KB9551.seq Check: 5031 from: 1 to: 4268
pF1KB9551 4268 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1306 1306
Size: 4268
Acc65I G'GTAC_C
Cuts at: 501 1284 501
Size: 783 3485
AclI AA'CG_TT
Cuts at: 4108 4108
Size: 4268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1074 2093 2525 2878 3540 1012
Size: 62 1019 432 353 662 1740
Fragments arranged by size:
1740 1019 662 432 353 62
AflIII A'CryG_T
Cuts at: 953 1620 3420 3701 953
Size: 667 1800 281 1520
Fragments arranged by size:
1800 1520 667 281
AleI CACnn'nnGTG
Cuts at: 367 367
Size: 4268
AlwNI CAG_nnn'CTG
Cuts at: 705 1183 1573 3011 705
Size: 478 390 1438 1962
Fragments arranged by size:
1962 1438 478 390
ApaLI G'TGCA_C
Cuts at: 3106 3106
Size: 4268
ApoI r'AATT_y
Cuts at: 1272 3621 3877 1272
Size: 2349 256 1663
Fragments arranged by size:
2349 1663 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4268
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
AvaI C'yCGr_G
Cuts at: 655 1288 655
Size: 633 3635
BamHI G'GATC_C
Cuts at: 1293 1293
Size: 4268
BanI G'GyrC_C
Cuts at: 501 1154 1284 1698 1946 1981 501
Size: 653 130 414 248 35 2788
Fragments arranged by size:
2788 653 414 248 130 35
BanII G_rGCy'C
Cuts at: 455 1282 2312 455
Size: 827 1030 2411
Fragments arranged by size:
2411 1030 827
BbeI G_GCGC'C
Cuts at: 1702 1950 1702
Size: 248 4020
BbsI GAAGACnn'nnnn_
Cuts at: 1084 1084
Size: 4268
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 117 151 117
Size: 34 4234
BciVI GTATCCnnnnn_n'
Cuts at: 340 2166 3222 4268 340
Size: 1826 1056 1046 340
Fragments arranged by size:
1826 1056 1046 340
BclI T'GATC_A
Cuts at: 1788 1788
Size: 4268
BglI GCCn_nnn'nGGC
Cuts at: 1249 1249
Size: 4268
BglII A'GATC_T
Cuts at: 1783 3820 1783
Size: 2037 2231
BlpI GC'TnA_GC
Cuts at: 124 1063 1380 124
Size: 939 317 3012
Fragments arranged by size:
3012 939 317
Bme1580I G_kGCm'C
Cuts at: 1159 1893 1986 3110 1159
Size: 734 93 1124 2317
Fragments arranged by size:
2317 1124 734 93
BmrI ACTGGGnnnn_n'
Cuts at: 1480 1561 1896 4040 1480
Size: 81 335 2144 1708
Fragments arranged by size:
2144 1708 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2635 2635
Size: 4268
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 279 889 1186 184
Size: 95 610 297 3266
Fragments arranged by size:
3266 610 297 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1444 2323 3037 3335 1444
Size: 879 714 298 2377
Fragments arranged by size:
2377 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3972 3972
Size: 4268
BsaAI yAC'GTr
Cuts at: 1621 1621
Size: 4268
BsaBI GATnn'nnATC
Cuts at: 6 1123 1782 3819 6
Size: 1117 659 2037 455
Fragments arranged by size:
2037 1117 659 455
BsaHI Gr'CG_yC
Cuts at: 713 748 1236 1699 1947 2649 4145 713
Size: 35 488 463 248 702 1496 836
Fragments arranged by size:
1496 836 702 488 463 248 35
BsaWI w'CCGG_w
Cuts at: 1127 1457 1978 2656 3067 3214 3488 1127
Size: 330 521 678 411 147 274 1907
Fragments arranged by size:
1907 678 521 411 330 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 862 892 997 1027 862
Size: 30 105 30 4103
Fragments arranged by size:
4103 105 30 30
BseYI C'CCAG_C
Cuts at: 624 1008 1693 2451 3116 3524 624
Size: 384 685 758 665 408 1368
Fragments arranged by size:
1368 758 685 665 408 384
BsiEI CG_ry'CG
Cuts at: 104 1810 1856 3086 104
Size: 1706 46 1230 1286
Fragments arranged by size:
1706 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1003 1282 2060 2250 3110 1003
Size: 279 778 190 860 2161
Fragments arranged by size:
2161 860 778 279 190
BsmBI CGTCTCn'nnnn_
Cuts at: 704 704
Size: 4268
Bsp1286I G_dGCh'C
Cuts at: 455 1003 1159 1282 1893 1986 2060 2250 2312
Size: 548 156 123 611 93 74 190 62
Cuts at: 2312 3110 455
Size: 798 1613
Fragments arranged by size:
1613 798 611 548 190 156 123 93
74 62
BspEI T'CCGG_A
Cuts at: 1127 1127
Size: 4268
BspHI T'CATG_A
Cuts at: 2700 2700
Size: 4268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1318 1532 1834 2215 1318
Size: 214 302 381 3371
Fragments arranged by size:
3371 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2560 2614 4098 4263 50
Size: 2510 54 1484 165 55
Fragments arranged by size:
2510 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2180 2180
Size: 4268
BsrFI r'CCGG_y
Cuts at: 2266 2447 2266
Size: 181 4087
BsrGI T'GTAC_A
Cuts at: 236 236
Size: 4268
BssSI C'ACGA_G
Cuts at: 1221 2539 3247 1221
Size: 1318 708 2242
Fragments arranged by size:
2242 1318 708
BstAPI GCAn_nnn'nTGC
Cuts at: 705 1645 705
Size: 940 3328
BstBI TT'CG_AA
Cuts at: 2629 2629
Size: 4268
BstXI CCAn_nnnn'nTGG
Cuts at: 678 678
Size: 4268
BstYI r'GATC_y
Cuts at: 1 1199 1293 1760 1783 2118 2364 2670 2682
Size: 1198 94 467 23 335 246 306 12
Cuts at: 2682 2768 3820 1
Size: 86 1052 449
Fragments arranged by size:
1198 1052 467 449 335 306 246 94
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 466 466
Size: 4268
BtgI C'CryG_G
Cuts at: 854 854
Size: 4268
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 801 1023 1154 2218 2399 2562 801
Size: 222 131 1064 181 163 2507
Fragments arranged by size:
2507 1064 222 181 163 131
BtsI GCAGTG_nn'
Cuts at: 1159 1511 1159
Size: 352 3916
ClaI AT'CG_AT
Cuts at: 692 692
Size: 4268
DraI TTT'AAA
Cuts at: 1267 1267
Size: 4268
DrdI GACnn_nn'nnGTC
Cuts at: 889 1974 3318 889
Size: 1085 1344 1839
Fragments arranged by size:
1839 1344 1085
EaeI y'GGCC_r
Cuts at: 1853 2027 2418 2445 3790 1853
Size: 174 391 27 1345 2331
Fragments arranged by size:
2331 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1853 1853
Size: 4268
EarI CTCTTCn'nnn_
Cuts at: 275 392 583 2291 2501 275
Size: 117 191 1708 210 2042
Fragments arranged by size:
2042 1708 210 191 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1242 2476 3218 3364 4078 1242
Size: 1234 742 146 714 1432
Fragments arranged by size:
1432 1234 742 714 146
EcoICRI GAG'CTC
Cuts at: 1280 1280
Size: 4268
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 279 889 1012 1074 1186 2093 2525 2878
Size: 95 610 123 62 112 907 432 353
Cuts at: 2878 3540 184
Size: 662 912
Fragments arranged by size:
912 907 662 610 432 353 123 112
95 62
EcoNI CCTnn'n_nnAGG
Cuts at: 465 465
Size: 4268
EcoO109I rG'GnC_Cy
Cuts at: 1407 1407
Size: 4268
EcoRI G'AATT_C
Cuts at: 1272 1272
Size: 4268
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 612 2287 612
Size: 1675 2593
FspI TGC'GCA
Cuts at: 2049 2049
Size: 4268
HaeII r_GCGC'y
Cuts at: 124 1702 1950 3180 3951 124
Size: 1578 248 1230 771 441
Fragments arranged by size:
1578 1230 771 441 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 631 663 824 856 862 894 1056 1088 1221
Size: 32 161 32 6 32 162 32 133
Cuts at: 1221 1253 2110 2140 2142 2172 631
Size: 32 857 30 2 30 2727
Fragments arranged by size:
2727 857 162 161 133 32 32 32
32 32 30 30 6 2
HincII GTy'rAC
Cuts at: 1307 1307
Size: 4268
KasI G'GCGC_C
Cuts at: 1698 1946 1698
Size: 248 4020
KpnI G_GTAC'C
Cuts at: 505 1288 505
Size: 783 3485
MluI A'CGCG_T
Cuts at: 3701 3701
Size: 4268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3027 3211 3027
Size: 184 4084
MscI TGG'CCA
Cuts at: 2029 2029
Size: 4268
MslI CAynn'nnrTG
Cuts at: 112 367 533 913 2257 2384 112
Size: 255 166 380 1344 127 1996
Fragments arranged by size:
1996 1344 380 255 166 127
MspA1I CmG'CkG
Cuts at: 162 628 798 1062 1379 1693 2053 2837 3082
Size: 466 170 264 317 314 360 784 245
Cuts at: 3082 162
Size: 1348
Fragments arranged by size:
1348 784 466 360 317 314 264 245
170
NaeI GCC'GGC
Cuts at: 2449 2449
Size: 4268
NarI GG'CG_CC
Cuts at: 1699 1947 1699
Size: 248 4020
NgoMIV G'CCGG_C
Cuts at: 2447 2447
Size: 4268
NspI r_CATG'y
Cuts at: 957 1321 1821 3424 957
Size: 364 500 1603 1801
Fragments arranged by size:
1801 1603 500 364
PciI A'CATG_T
Cuts at: 953 3420 953
Size: 2467 1801
PfoI T'CCnGG_A
Cuts at: 866 866
Size: 4268
PmeI GTTT'AAAC
Cuts at: 1267 1267
Size: 4268
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2714 2746 2714
Size: 32 4236
PstI C_TGCA'G
Cuts at: 1315 2000 1315
Size: 685 3583
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4268
PvuII CAG'CTG
Cuts at: 162 628 1062 1693 2053 162
Size: 466 434 631 360 2377
Fragments arranged by size:
2377 631 466 434 360
RsrII CG'GwC_CG
Cuts at: 2463 2463
Size: 4268
SacI G_AGCT'C
Cuts at: 1282 1282
Size: 4268
SalI G'TCGA_C
Cuts at: 1305 1305
Size: 4268
SapI GCTCTTCn'nnn_
Cuts at: 2291 2501 2291
Size: 210 4058
SbfI CC_TGCA'GG
Cuts at: 1315 1315
Size: 4268
ScaI AGT'ACT
Cuts at: 3785 3785
Size: 4268
SfcI C'TryA_G
Cuts at: 33 1311 1996 2964 3155 33
Size: 1278 685 968 191 1146
Fragments arranged by size:
1278 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1700 1948 1700
Size: 248 4020
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4268
SmaI CCC'GGG
Cuts at: 1290 1290
Size: 4268
SmlI C'TyrA_G
Cuts at: 1423 2338 3052 3314 1423
Size: 915 714 262 2377
Fragments arranged by size:
2377 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4268
SphI G_CATG'C
Cuts at: 1321 1821 1321
Size: 500 3768
StyI C'CwwG_G
Cuts at: 1402 1756 1402
Size: 354 3914
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1851 1984 2443 2652 1851
Size: 133 459 209 3467
Fragments arranged by size:
3467 459 209 133
TatI w'GTAC_w
Cuts at: 236 425 3783 236
Size: 189 3358 721
Fragments arranged by size:
3358 721 189
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1138 1170 3785 3817 1138
Size: 32 2615 32 1589
Fragments arranged by size:
2615 1589 32 32
Tth111I GACn'n_nGTC
Cuts at: 2065 2065
Size: 4268
XbaI T'CTAG_A
Cuts at: 1299 1299
Size: 4268
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1763 114
Size: 1649 2619
XmaI C'CCGG_G
Cuts at: 1288 1288
Size: 4268
XmnI GAAnn'nnTTC
Cuts at: 401 924 401
Size: 523 3745
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI
Bpu10I BseRI BsgI BsiWI BsmI BssHII BstEII BstZ17I
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI