Restriction map for
pF1KB9550
(Circular) (Six-base) MAPSORT of: pF1KB9550.seq Check: 9804 from: 1 to: 4064
pF1KB9550 4064 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 821 1102 821
Size: 281 3783
Acc65I G'GTAC_C
Cuts at: 1080 1080
Size: 4064
AclI AA'CG_TT
Cuts at: 872 3904 872
Size: 3032 1032
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 1889 2321 2674 3336 457
Size: 1432 432 353 662 1185
Fragments arranged by size:
1432 1185 662 432 353
AflIII A'CryG_T
Cuts at: 599 1416 3216 3497 599
Size: 817 1800 281 1166
Fragments arranged by size:
1800 1166 817 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 599 599
Size: 4064
AlwNI CAG_nnn'CTG
Cuts at: 1369 2807 1369
Size: 1438 2626
ApaLI G'TGCA_C
Cuts at: 2902 2902
Size: 4064
ApoI r'AATT_y
Cuts at: 156 1068 3417 3673 156
Size: 912 2349 256 547
Fragments arranged by size:
2349 912 547 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4064
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
AvaI C'yCGr_G
Cuts at: 1084 1084
Size: 4064
BamHI G'GATC_C
Cuts at: 1089 1089
Size: 4064
BanI G'GyrC_C
Cuts at: 798 1080 1494 1742 1777 798
Size: 282 414 248 35 3085
Fragments arranged by size:
3085 414 282 248 35
BanII G_rGCy'C
Cuts at: 320 431 560 622 1078 2108 320
Size: 111 129 62 456 1030 2276
Fragments arranged by size:
2276 1030 456 129 111 62
BbeI G_GCGC'C
Cuts at: 1498 1746 1498
Size: 248 3816
BbvCI CC'TCA_GC
Cuts at: 932 932
Size: 4064
BciVI GTATCCnnnnn_n'
Cuts at: 1962 3018 4064 1962
Size: 1056 1046 1962
Fragments arranged by size:
1962 1056 1046
BclI T'GATC_A
Cuts at: 981 1584 981
Size: 603 3461
BglI GCCn_nnn'nGGC
Cuts at: 387 387
Size: 4064
BglII A'GATC_T
Cuts at: 1579 3616 1579
Size: 2037 2027
BlpI GC'TnA_GC
Cuts at: 1176 1176
Size: 4064
Bme1580I G_kGCm'C
Cuts at: 1689 1782 2906 1689
Size: 93 1124 2847
Fragments arranged by size:
2847 1124 93
BmgBI CAC'GTC
Cuts at: 600 663 600
Size: 63 4001
BmrI ACTGGGnnnn_n'
Cuts at: 660 1276 1357 1692 3836 660
Size: 616 81 335 2144 888
Fragments arranged by size:
2144 888 616 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2431 2431
Size: 4064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 445 1019 286
Size: 159 574 3331
Fragments arranged by size:
3331 574 159
Bpu10I CC'TnA_GC
Cuts at: 932 932
Size: 4064
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 274 1014 1240 2119 2833 3131 134
Size: 140 740 226 879 714 298 1067
Fragments arranged by size:
1067 879 740 714 298 226 140
BsaI GGTCTCn'nnnn_
Cuts at: 363 3768 363
Size: 3405 659
BsaAI yAC'GTr
Cuts at: 1417 1417
Size: 4064
BsaBI GATnn'nnATC
Cuts at: 6 1578 3615 6
Size: 1572 2037 455
Fragments arranged by size:
2037 1572 455
BsaHI Gr'CG_yC
Cuts at: 1495 1743 2445 3941 1495
Size: 248 702 1496 1618
Fragments arranged by size:
1618 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1253 1774 2452 2863 3010 3284 1253
Size: 521 678 411 147 274 2033
Fragments arranged by size:
2033 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1017 1047 1017
Size: 30 4034
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 511 949 511
Size: 438 3626
BseYI C'CCAG_C
Cuts at: 1489 2247 2912 3320 1489
Size: 758 665 408 2233
Fragments arranged by size:
2233 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 185
Size: 4064
BsiEI CG_ry'CG
Cuts at: 104 1606 1652 2882 104
Size: 1502 46 1230 1286
Fragments arranged by size:
1502 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1078 1856 2046 2906 1078
Size: 778 190 860 2236
Fragments arranged by size:
2236 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 930 930
Size: 4064
Bsp1286I G_dGCh'C
Cuts at: 320 431 560 622 1078 1689 1782 1856 2046
Size: 111 129 62 456 611 93 74 190
Cuts at: 2046 2108 2906 320
Size: 62 798 1478
Fragments arranged by size:
1478 798 611 456 190 129 111 93
74 62 62
BspHI T'CATG_A
Cuts at: 2496 2496
Size: 4064
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1114 1328 1630 2011 1114
Size: 214 302 381 3167
Fragments arranged by size:
3167 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2356 2410 3894 4059 50
Size: 2306 54 1484 165 55
Fragments arranged by size:
2306 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1976 1976
Size: 4064
BsrFI r'CCGG_y
Cuts at: 2062 2243 2062
Size: 181 3883
BssSI C'ACGA_G
Cuts at: 2335 3043 2335
Size: 708 3356
BstAPI GCAn_nnn'nTGC
Cuts at: 284 1441 284
Size: 1157 2907
BstBI TT'CG_AA
Cuts at: 2425 2425
Size: 4064
BstYI r'GATC_y
Cuts at: 1 330 725 1089 1556 1579 1914 2160 2466
Size: 329 395 364 467 23 335 246 306
Cuts at: 2466 2478 2564 3616 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 467 449 395 364 335 329 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 538 538
Size: 4064
BtgI C'CryG_G
Cuts at: 321 353 321
Size: 32 4032
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2014 2195 2358 2014
Size: 181 163 3720
Fragments arranged by size:
3720 181 163
BtsI GCAGTG_nn'
Cuts at: 194 711 940 1307 194
Size: 517 229 367 2951
Fragments arranged by size:
2951 517 367 229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 726 761 726
Size: 35 4029
DraI TTT'AAA
Cuts at: 1063 1063
Size: 4064
DrdI GACnn_nn'nnGTC
Cuts at: 691 1770 3114 691
Size: 1079 1344 1641
Fragments arranged by size:
1641 1344 1079
EaeI y'GGCC_r
Cuts at: 639 1649 1823 2214 2241 3586 639
Size: 1010 174 391 27 1345 1117
Fragments arranged by size:
1345 1117 1010 391 174 27
EagI C'GGCC_G
Cuts at: 1649 1649
Size: 4064
EarI CTCTTCn'nnn_
Cuts at: 211 2087 2297 211
Size: 1876 210 1978
Fragments arranged by size:
1978 1876 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2272 3014 3160 3874 2272
Size: 742 146 714 2462
Fragments arranged by size:
2462 742 714 146
EcoICRI GAG'CTC
Cuts at: 1076 1076
Size: 4064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 445 457 1019 1889 2321 2674 3336 286
Size: 159 12 562 870 432 353 662 1014
Fragments arranged by size:
1014 870 662 562 432 353 159 12
EcoO109I rG'GnC_Cy
Cuts at: 684 1203 684
Size: 519 3545
EcoRI G'AATT_C
Cuts at: 1068 1068
Size: 4064
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2083 2083
Size: 4064
FspI TGC'GCA
Cuts at: 1845 1845
Size: 4064
HaeII r_GCGC'y
Cuts at: 1498 1746 2976 3747 1498
Size: 248 1230 771 1815
Fragments arranged by size:
1815 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 266 298 987 1019 1906 1936 1938 1968 266
Size: 32 689 32 887 30 2 30 2362
Fragments arranged by size:
2362 887 689 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1103 1103
Size: 4064
KasI G'GCGC_C
Cuts at: 1494 1742 1494
Size: 248 3816
KpnI G_GTAC'C
Cuts at: 1084 1084
Size: 4064
MluI A'CGCG_T
Cuts at: 3497 3497
Size: 4064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2823 3007 2823
Size: 184 3880
MscI TGG'CCA
Cuts at: 1825 1825
Size: 4064
MslI CAynn'nnrTG
Cuts at: 2053 2180 2053
Size: 127 3937
MspA1I CmG'CkG
Cuts at: 224 732 804 1175 1489 1849 2633 2878 224
Size: 508 72 371 314 360 784 245 1410
Fragments arranged by size:
1410 784 508 371 360 314 245 72
NaeI GCC'GGC
Cuts at: 2245 2245
Size: 4064
NarI GG'CG_CC
Cuts at: 1495 1743 1495
Size: 248 3816
NcoI C'CATG_G
Cuts at: 321 353 321
Size: 32 4032
NgoMIV G'CCGG_C
Cuts at: 2243 2243
Size: 4064
NspI r_CATG'y
Cuts at: 867 1117 1617 3220 867
Size: 250 500 1603 1711
Fragments arranged by size:
1711 1603 500 250
PciI A'CATG_T
Cuts at: 3216 3216
Size: 4064
PflMI CCAn_nnn'nTGG
Cuts at: 976 976
Size: 4064
PmeI GTTT'AAAC
Cuts at: 1063 1063
Size: 4064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 692 724 2510 2542 692
Size: 32 1786 32 2214
Fragments arranged by size:
2214 1786 32 32
PpuMI rG'GwC_Cy
Cuts at: 684 684
Size: 4064
PstI C_TGCA'G
Cuts at: 311 700 1111 1796 311
Size: 389 411 685 2579
Fragments arranged by size:
2579 685 411 389
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4064
PvuII CAG'CTG
Cuts at: 224 732 1489 1849 224
Size: 508 757 360 2439
Fragments arranged by size:
2439 757 508 360
RsrII CG'GwC_CG
Cuts at: 2259 2259
Size: 4064
SacI G_AGCT'C
Cuts at: 1078 1078
Size: 4064
SalI G'TCGA_C
Cuts at: 1101 1101
Size: 4064
SapI GCTCTTCn'nnn_
Cuts at: 2087 2297 2087
Size: 210 3854
SbfI CC_TGCA'GG
Cuts at: 1111 1111
Size: 4064
ScaI AGT'ACT
Cuts at: 3581 3581
Size: 4064
SfcI C'TryA_G
Cuts at: 33 307 448 696 1107 1792 2760 2951 33
Size: 274 141 248 411 685 968 191 1146
Fragments arranged by size:
1146 968 685 411 274 248 191 141
SfoI GGC'GCC
Cuts at: 1496 1744 1496
Size: 248 3816
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4064
SmaI CCC'GGG
Cuts at: 1086 1086
Size: 4064
SmlI C'TyrA_G
Cuts at: 149 289 993 1219 2134 2848 3110 149
Size: 140 704 226 915 714 262 1103
Fragments arranged by size:
1103 915 714 704 262 226 140
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4064
SphI G_CATG'C
Cuts at: 867 1117 1617 867
Size: 250 500 3314
Fragments arranged by size:
3314 500 250
StyI C'CwwG_G
Cuts at: 321 353 460 491 1198 1552 321
Size: 32 107 31 707 354 2833
Fragments arranged by size:
2833 707 354 107 32 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1647 1780 2239 2448 1647
Size: 133 459 209 3263
Fragments arranged by size:
3263 459 209 133
TatI w'GTAC_w
Cuts at: 172 3579 172
Size: 3407 657
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3581 3613 3581
Size: 32 4032
Tth111I GACn'n_nGTC
Cuts at: 1861 1861
Size: 4064
XbaI T'CTAG_A
Cuts at: 1095 1095
Size: 4064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1559 1559
Size: 4064
XmaI C'CCGG_G
Cuts at: 1084 1084
Size: 4064
XmnI GAAnn'nnTTC
Cuts at: 707 707
Size: 4064
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BmtI
BsiWI BsmI BspEI BsrGI BssHII BstEII BstXI BstZ17I
ClaI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI