Restriction map for
pF1KB9544
(Circular) (Six-base) MAPSORT of: pF1KB9544.seq Check: 3514 from: 1 to: 4916
pF1KB9544 4916 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 421 1954 421
Size: 1533 3383
Acc65I G'GTAC_C
Cuts at: 1932 1932
Size: 4916
AclI AA'CG_TT
Cuts at: 210 4756 210
Size: 4546 370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 920 1675 2741 3173 3526 4188 920
Size: 755 1066 432 353 662 1648
Fragments arranged by size:
1648 1066 755 662 432 353
AflIII A'CryG_T
Cuts at: 1337 2268 4068 4349 1337
Size: 931 1800 281 1904
Fragments arranged by size:
1904 1800 931 281
AleI CACnn'nnGTG
Cuts at: 1528 1528
Size: 4916
AlwNI CAG_nnn'CTG
Cuts at: 1494 2221 3659 1494
Size: 727 1438 2751
Fragments arranged by size:
2751 1438 727
ApaLI G'TGCA_C
Cuts at: 3754 3754
Size: 4916
ApoI r'AATT_y
Cuts at: 264 1920 4269 4525 264
Size: 1656 2349 256 655
Fragments arranged by size:
2349 1656 655 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4916
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4916
AvaI C'yCGr_G
Cuts at: 791 1936 791
Size: 1145 3771
AvrII C'CTAG_G
Cuts at: 816 816
Size: 4916
BamHI G'GATC_C
Cuts at: 1941 1941
Size: 4916
BanI G'GyrC_C
Cuts at: 1932 2346 2594 2629 1932
Size: 414 248 35 4219
Fragments arranged by size:
4219 414 248 35
BanII G_rGCy'C
Cuts at: 1670 1930 2960 1670
Size: 260 1030 3626
Fragments arranged by size:
3626 1030 260
BbeI G_GCGC'C
Cuts at: 2350 2598 2350
Size: 248 4668
BbvCI CC'TCA_GC
Cuts at: 1433 1433
Size: 4916
BciVI GTATCCnnnnn_n'
Cuts at: 2814 3870 4916 2814
Size: 1056 1046 2814
Fragments arranged by size:
2814 1056 1046
BclI T'GATC_A
Cuts at: 153 2436 153
Size: 2283 2633
BglI GCCn_nnn'nGGC
Cuts at: 902 902
Size: 4916
BglII A'GATC_T
Cuts at: 2431 4468 2431
Size: 2037 2879
BlpI GC'TnA_GC
Cuts at: 2028 2028
Size: 4916
Bme1580I G_kGCm'C
Cuts at: 1803 2541 2634 3758 1803
Size: 738 93 1124 2961
Fragments arranged by size:
2961 1124 738 93
BmrI ACTGGGnnnn_n'
Cuts at: 506 918 2128 2209 2544 4688 506
Size: 412 1210 81 335 2144 734
Fragments arranged by size:
2144 1210 734 412 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 525 3283 525
Size: 2758 2158
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1235 1235
Size: 4916
Bpu10I CC'TnA_GC
Cuts at: 254 1433 1711 254
Size: 1179 278 3459
Fragments arranged by size:
3459 1179 278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2092 2971 3685 3983 2092
Size: 879 714 298 3025
Fragments arranged by size:
3025 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4620 4620
Size: 4916
BsaAI yAC'GTr
Cuts at: 2269 2269
Size: 4916
BsaBI GATnn'nnATC
Cuts at: 6 152 2430 4467 6
Size: 146 2278 2037 455
Fragments arranged by size:
2278 2037 455 146
BsaHI Gr'CG_yC
Cuts at: 917 2347 2595 3297 4793 917
Size: 1430 248 702 1496 1040
Fragments arranged by size:
1496 1430 1040 702 248
BsaWI w'CCGG_w
Cuts at: 2105 2626 3304 3715 3862 4136 2105
Size: 521 678 411 147 274 2885
Fragments arranged by size:
2885 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 333 745 1184 1328 333
Size: 412 439 144 3921
Fragments arranged by size:
3921 439 412 144
BseYI C'CCAG_C
Cuts at: 1018 1198 2341 3099 3764 4172 1018
Size: 180 1143 758 665 408 1762
Fragments arranged by size:
1762 1143 758 665 408 180
BsiEI CG_ry'CG
Cuts at: 104 2458 2504 3734 104
Size: 2354 46 1230 1286
Fragments arranged by size:
2354 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 548 1930 2708 2898 3758 548
Size: 1382 778 190 860 1706
Fragments arranged by size:
1706 1382 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 548 1670 1803 1930 2541 2634 2708 2898 2960
Size: 1122 133 127 611 93 74 190 62
Cuts at: 2960 3758 548
Size: 798 1706
Fragments arranged by size:
1706 1122 798 611 190 133 127 93
74 62
BspHI T'CATG_A
Cuts at: 3348 3348
Size: 4916
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1966 2180 2482 2863 1966
Size: 214 302 381 4019
Fragments arranged by size:
4019 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3208 3262 4746 4911 50
Size: 3158 54 1484 165 55
Fragments arranged by size:
3158 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1886 2828 1886
Size: 942 3974
BsrFI r'CCGG_y
Cuts at: 2914 3095 2914
Size: 181 4735
BssSI C'ACGA_G
Cuts at: 3187 3895 3187
Size: 708 4208
BstAPI GCAn_nnn'nTGC
Cuts at: 2293 2293
Size: 4916
BstBI TT'CG_AA
Cuts at: 3277 3277
Size: 4916
BstXI CCAn_nnnn'nTGG
Cuts at: 1258 1258
Size: 4916
BstYI r'GATC_y
Cuts at: 1 1941 2408 2431 2766 3012 3318 3330 3416
Size: 1940 467 23 335 246 306 12 86
Cuts at: 3416 4468 1
Size: 1052 449
Fragments arranged by size:
1940 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4916
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2866 3047 3210 2866
Size: 181 163 4572
Fragments arranged by size:
4572 181 163
BtsI GCAGTG_nn'
Cuts at: 579 2159 579
Size: 1580 3336
DraI TTT'AAA
Cuts at: 677 1915 677
Size: 1238 3678
DrdI GACnn_nn'nnGTC
Cuts at: 2622 3966 2622
Size: 1344 3572
EaeI y'GGCC_r
Cuts at: 186 2501 2675 3066 3093 4438 186
Size: 2315 174 391 27 1345 664
Fragments arranged by size:
2315 1345 664 391 174 27
EagI C'GGCC_G
Cuts at: 2501 2501
Size: 4916
EarI CTCTTCn'nnn_
Cuts at: 230 325 522 1072 1283 1584 2939 3149 230
Size: 95 197 550 211 301 1355 210 1997
Fragments arranged by size:
1997 1355 550 301 211 210 197 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3124 3866 4012 4726 3124
Size: 742 146 714 3314
Fragments arranged by size:
3314 742 714 146
EcoICRI GAG'CTC
Cuts at: 1928 1928
Size: 4916
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 920 1235 1675 2741 3173 3526 4188 920
Size: 315 440 1066 432 353 662 1648
Fragments arranged by size:
1648 1066 662 440 432 353 315
EcoO109I rG'GnC_Cy
Cuts at: 2055 2055
Size: 4916
EcoRI G'AATT_C
Cuts at: 264 1920 264
Size: 1656 3260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2935 2935
Size: 4916
FspI TGC'GCA
Cuts at: 2697 2697
Size: 4916
HaeII r_GCGC'y
Cuts at: 2350 2598 3828 4599 2350
Size: 248 1230 771 2667
Fragments arranged by size:
2667 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 358 390 586 618 718 750 982 1014 2758
Size: 32 196 32 100 32 232 32 1744
Cuts at: 2758 2788 2790 2820 358
Size: 30 2 30 2454
Fragments arranged by size:
2454 1744 232 196 100 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1955 1955
Size: 4916
KasI G'GCGC_C
Cuts at: 2346 2594 2346
Size: 248 4668
KpnI G_GTAC'C
Cuts at: 1936 1936
Size: 4916
MluI A'CGCG_T
Cuts at: 4349 4349
Size: 4916
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3675 3859 3675
Size: 184 4732
MscI TGG'CCA
Cuts at: 2677 2677
Size: 4916
MslI CAynn'nnrTG
Cuts at: 1256 1528 1624 2905 3032 1256
Size: 272 96 1281 127 3140
Fragments arranged by size:
3140 1281 272 127 96
MspA1I CmG'CkG
Cuts at: 184 1695 2027 2341 2701 3485 3730 184
Size: 1511 332 314 360 784 245 1370
Fragments arranged by size:
1511 1370 784 360 332 314 245
NaeI GCC'GGC
Cuts at: 3097 3097
Size: 4916
NarI GG'CG_CC
Cuts at: 2347 2595 2347
Size: 248 4668
NcoI C'CATG_G
Cuts at: 107 107
Size: 4916
NgoMIV G'CCGG_C
Cuts at: 3095 3095
Size: 4916
NspI r_CATG'y
Cuts at: 1341 1820 1969 2469 4072 1341
Size: 479 149 500 1603 2185
Fragments arranged by size:
2185 1603 500 479 149
PciI A'CATG_T
Cuts at: 1337 4068 1337
Size: 2731 2185
PflMI CCAn_nnn'nTGG
Cuts at: 1213 1243 1213
Size: 30 4886
PmeI GTTT'AAAC
Cuts at: 1915 1915
Size: 4916
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 4884
PstI C_TGCA'G
Cuts at: 1499 1736 1963 2648 1499
Size: 237 227 685 3767
Fragments arranged by size:
3767 685 237 227
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4916
PvuII CAG'CTG
Cuts at: 184 1695 2341 2701 184
Size: 1511 646 360 2399
Fragments arranged by size:
2399 1511 646 360
RsrII CG'GwC_CG
Cuts at: 3111 3111
Size: 4916
SacI G_AGCT'C
Cuts at: 1930 1930
Size: 4916
SalI G'TCGA_C
Cuts at: 1953 1953
Size: 4916
SapI GCTCTTCn'nnn_
Cuts at: 522 1072 2939 3149 522
Size: 550 1867 210 2289
Fragments arranged by size:
2289 1867 550 210
SbfI CC_TGCA'GG
Cuts at: 1963 1963
Size: 4916
ScaI AGT'ACT
Cuts at: 1592 4433 1592
Size: 2841 2075
SexAI A'CCwGG_T
Cuts at: 1349 1349
Size: 4916
SfcI C'TryA_G
Cuts at: 33 1495 1583 1732 1959 2644 3612 3803 33
Size: 1462 88 149 227 685 968 191 1146
Fragments arranged by size:
1462 1146 968 685 227 191 149 88
SfoI GGC'GCC
Cuts at: 2348 2596 2348
Size: 248 4668
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4916
SmaI CCC'GGG
Cuts at: 1938 1938
Size: 4916
SmlI C'TyrA_G
Cuts at: 2071 2986 3700 3962 2071
Size: 915 714 262 3025
Fragments arranged by size:
3025 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4916
SphI G_CATG'C
Cuts at: 1969 2469 1969
Size: 500 4416
StyI C'CwwG_G
Cuts at: 107 816 1803 1811 2050 2404 107
Size: 709 987 8 239 354 2619
Fragments arranged by size:
2619 987 709 354 239 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1632 2499 2632 3091 3300 1632
Size: 867 133 459 209 3248
Fragments arranged by size:
3248 867 459 209 133
TatI w'GTAC_w
Cuts at: 1590 4431 1590
Size: 2841 2075
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 853 885 1114 1146 4433 4465 853
Size: 32 229 32 3287 32 1304
Fragments arranged by size:
3287 1304 229 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1727 2713 1727
Size: 986 3930
XbaI T'CTAG_A
Cuts at: 1947 1947
Size: 4916
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2411 2411
Size: 4916
XmaI C'CCGG_G
Cuts at: 1936 1936
Size: 4916
XmnI GAAnn'nnTTC
Cuts at: 974 974
Size: 4916
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI BaeI BbsI BcgI BfrBI BmgBI BmtI
BsaXI BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI