Restriction map for
pF1KB9542
(Circular) (Six-base) MAPSORT of: pF1KB9542.seq Check: 4014 from: 1 to: 3983
pF1KB9542 3983 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1021 1021
Size: 3983
Acc65I G'GTAC_C
Cuts at: 999 999
Size: 3983
AclI AA'CG_TT
Cuts at: 3823 3823
Size: 3983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 382 776 902 1808 2240 2593 3255 172
Size: 210 394 126 906 432 353 662 900
Fragments arranged by size:
906 900 662 432 394 353 210 126
AfeI AGC'GCT
Cuts at: 626 626
Size: 3983
AflIII A'CryG_T
Cuts at: 241 606 1335 3135 3416 241
Size: 365 729 1800 281 808
Fragments arranged by size:
1800 808 729 365 281
AgeI A'CCGG_T
Cuts at: 899 899
Size: 3983
AlwNI CAG_nnn'CTG
Cuts at: 753 1288 2726 753
Size: 535 1438 2010
Fragments arranged by size:
2010 1438 535
ApaLI G'TGCA_C
Cuts at: 2821 2821
Size: 3983
ApoI r'AATT_y
Cuts at: 987 3336 3592 987
Size: 2349 256 1378
Fragments arranged by size:
2349 1378 256
AseI AT'TA_AT
Cuts at: 20 260 313 20
Size: 240 53 3690
Fragments arranged by size:
3690 240 53
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
AvaI C'yCGr_G
Cuts at: 1003 1003
Size: 3983
BamHI G'GATC_C
Cuts at: 1008 1008
Size: 3983
BanI G'GyrC_C
Cuts at: 871 999 1413 1661 1696 871
Size: 128 414 248 35 3158
Fragments arranged by size:
3158 414 248 128 35
BanII G_rGCy'C
Cuts at: 997 2027 997
Size: 1030 2953
BbeI G_GCGC'C
Cuts at: 1417 1665 1417
Size: 248 3735
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3949
BciVI GTATCCnnnnn_n'
Cuts at: 1881 2937 3983 1881
Size: 1056 1046 1881
Fragments arranged by size:
1881 1056 1046
BclI T'GATC_A
Cuts at: 1503 1503
Size: 3983
BglI GCCn_nnn'nGGC
Cuts at: 507 507
Size: 3983
BglII A'GATC_T
Cuts at: 582 1498 3535 582
Size: 916 2037 1030
Fragments arranged by size:
2037 1030 916
BlpI GC'TnA_GC
Cuts at: 1095 1095
Size: 3983
Bme1580I G_kGCm'C
Cuts at: 1608 1701 2825 1608
Size: 93 1124 2766
Fragments arranged by size:
2766 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1195 1276 1611 3755 1195
Size: 81 335 2144 1423
Fragments arranged by size:
2144 1423 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 3983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 838
Size: 3983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 931 1159 2038 2752 3050 607
Size: 324 228 879 714 298 1540
Fragments arranged by size:
1540 879 714 324 298 228
BsaI GGTCTCn'nnnn_
Cuts at: 125 733 3687 125
Size: 608 2954 421
Fragments arranged by size:
2954 608 421
BsaAI yAC'GTr
Cuts at: 1336 1336
Size: 3983
BsaBI GATnn'nnATC
Cuts at: 6 1497 3534 6
Size: 1491 2037 455
Fragments arranged by size:
2037 1491 455
BsaHI Gr'CG_yC
Cuts at: 1414 1662 2364 3860 1414
Size: 248 702 1496 1537
Fragments arranged by size:
1537 1496 702 248
BsaWI w'CCGG_w
Cuts at: 899 1172 1693 2371 2782 2929 3203 899
Size: 273 521 678 411 147 274 1679
Fragments arranged by size:
1679 678 521 411 274 273 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 949 949
Size: 3983
BseYI C'CCAG_C
Cuts at: 136 267 455 822 1408 2166 2831 3239 136
Size: 131 188 367 586 758 665 408 880
Fragments arranged by size:
880 758 665 586 408 367 188 131
BsiEI CG_ry'CG
Cuts at: 104 1525 1571 2801 104
Size: 1421 46 1230 1286
Fragments arranged by size:
1421 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 891 997 1775 1965 2825 891
Size: 106 778 190 860 2049
Fragments arranged by size:
2049 860 778 190 106
BsmBI CGTCTCn'nnnn_
Cuts at: 120 120
Size: 3983
Bsp1286I G_dGCh'C
Cuts at: 891 997 1608 1701 1775 1965 2027 2825 891
Size: 106 611 93 74 190 62 798 2049
Fragments arranged by size:
2049 798 611 190 106 93 74 62
BspHI T'CATG_A
Cuts at: 2415 2415
Size: 3983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1033 1247 1549 1930 1033
Size: 214 302 381 3086
Fragments arranged by size:
3086 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2275 2329 3813 3978 50
Size: 2225 54 1484 165 55
Fragments arranged by size:
2225 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1895 1895
Size: 3983
BsrFI r'CCGG_y
Cuts at: 210 899 1981 2162 210
Size: 689 1082 181 2031
Fragments arranged by size:
2031 1082 689 181
BssSI C'ACGA_G
Cuts at: 931 2254 2962 931
Size: 1323 708 1952
Fragments arranged by size:
1952 1323 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1360 1360
Size: 3983
BstBI TT'CG_AA
Cuts at: 2344 2344
Size: 3983
BstEII G'GTnAC_C
Cuts at: 742 742
Size: 3983
BstXI CCAn_nnnn'nTGG
Cuts at: 640 782 640
Size: 142 3841
BstYI r'GATC_y
Cuts at: 1 582 1008 1475 1498 1833 2079 2385 2397
Size: 581 426 467 23 335 246 306 12
Cuts at: 2397 2483 3535 1
Size: 86 1052 449
Fragments arranged by size:
1052 581 467 449 426 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 723 723
Size: 3983
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1933 2114 2277 1933
Size: 181 163 3639
Fragments arranged by size:
3639 181 163
BtsI GCAGTG_nn'
Cuts at: 1226 1226
Size: 3983
DraI TTT'AAA
Cuts at: 982 982
Size: 3983
DraIII CAC_nnn'GTG
Cuts at: 765 765
Size: 3983
DrdI GACnn_nn'nnGTC
Cuts at: 1689 3033 1689
Size: 1344 2639
EaeI y'GGCC_r
Cuts at: 1568 1742 2133 2160 3505 1568
Size: 174 391 27 1345 2046
Fragments arranged by size:
2046 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1568 1568
Size: 3983
EarI CTCTTCn'nnn_
Cuts at: 409 2006 2216 409
Size: 1597 210 2176
Fragments arranged by size:
2176 1597 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 565 2191 2933 3079 3793 565
Size: 1626 742 146 714 755
Fragments arranged by size:
1626 755 742 714 146
EcoICRI GAG'CTC
Cuts at: 995 995
Size: 3983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 382 776 838 902 1808 2240 2593 3255
Size: 210 394 62 64 906 432 353 662
Cuts at: 3255 172
Size: 900
Fragments arranged by size:
906 900 662 432 394 353 210 64
62
EcoNI CCTnn'n_nnAGG
Cuts at: 524 524
Size: 3983
EcoO109I rG'GnC_Cy
Cuts at: 797 1122 797
Size: 325 3658
EcoRI G'AATT_C
Cuts at: 987 987
Size: 3983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2002 2002
Size: 3983
FspI TGC'GCA
Cuts at: 1764 1764
Size: 3983
HaeII r_GCGC'y
Cuts at: 628 1417 1665 2895 3666 628
Size: 789 248 1230 771 945
Fragments arranged by size:
1230 945 789 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 152 184 1825 1855 1857 1887 152
Size: 32 1641 30 2 30 2248
Fragments arranged by size:
2248 1641 32 30 30 2
HincII GTy'rAC
Cuts at: 1022 1022
Size: 3983
HindIII A'AGCT_T
Cuts at: 340 340
Size: 3983
KasI G'GCGC_C
Cuts at: 1413 1661 1413
Size: 248 3735
KpnI G_GTAC'C
Cuts at: 1003 1003
Size: 3983
MluI A'CGCG_T
Cuts at: 3416 3416
Size: 3983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 183 683 2742 2926 183
Size: 500 2059 184 1240
Fragments arranged by size:
2059 1240 500 184
MscI TGG'CCA
Cuts at: 1744 1744
Size: 3983
MslI CAynn'nnrTG
Cuts at: 229 384 1972 2099 229
Size: 155 1588 127 2113
Fragments arranged by size:
2113 1588 155 127
MspA1I CmG'CkG
Cuts at: 1094 1408 1768 2552 2797 1094
Size: 314 360 784 245 2280
Fragments arranged by size:
2280 784 360 314 245
NaeI GCC'GGC
Cuts at: 212 2164 212
Size: 1952 2031
NarI GG'CG_CC
Cuts at: 1414 1662 1414
Size: 248 3735
NcoI C'CATG_G
Cuts at: 723 723
Size: 3983
NgoMIV G'CCGG_C
Cuts at: 210 2162 210
Size: 1952 2031
NspI r_CATG'y
Cuts at: 245 610 1036 1536 3139 245
Size: 365 426 500 1603 1089
Fragments arranged by size:
1603 1089 500 426 365
PciI A'CATG_T
Cuts at: 241 606 3135 241
Size: 365 2529 1089
Fragments arranged by size:
2529 1089 365
PflMI CCAn_nnn'nTGG
Cuts at: 227 765 227
Size: 538 3445
PmeI GTTT'AAAC
Cuts at: 982 982
Size: 3983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2429 2461 2429
Size: 32 3951
PpuMI rG'GwC_Cy
Cuts at: 797 797
Size: 3983
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 534 566 534
Size: 32 3951
PstI C_TGCA'G
Cuts at: 525 1030 1715 525
Size: 505 685 2793
Fragments arranged by size:
2793 685 505
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3983
PvuII CAG'CTG
Cuts at: 1408 1768 1408
Size: 360 3623
RsrII CG'GwC_CG
Cuts at: 2178 2178
Size: 3983
SacI G_AGCT'C
Cuts at: 997 997
Size: 3983
SalI G'TCGA_C
Cuts at: 1020 1020
Size: 3983
SanDI GG'GwC_CC
Cuts at: 797 797
Size: 3983
SapI GCTCTTCn'nnn_
Cuts at: 2006 2216 2006
Size: 210 3773
SbfI CC_TGCA'GG
Cuts at: 525 1030 525
Size: 505 3478
ScaI AGT'ACT
Cuts at: 3500 3500
Size: 3983
SfcI C'TryA_G
Cuts at: 33 521 1026 1711 2679 2870 33
Size: 488 505 685 968 191 1146
Fragments arranged by size:
1146 968 685 505 488 191
SfoI GGC'GCC
Cuts at: 1415 1663 1415
Size: 248 3735
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3983
SmaI CCC'GGG
Cuts at: 1005 1005
Size: 3983
SmlI C'TyrA_G
Cuts at: 586 946 1138 2053 2767 3029 586
Size: 360 192 915 714 262 1540
Fragments arranged by size:
1540 915 714 360 262 192
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3983
SphI G_CATG'C
Cuts at: 1036 1536 1036
Size: 500 3483
StuI AGG'CCT
Cuts at: 519 519
Size: 3983
StyI C'CwwG_G
Cuts at: 723 1117 1471 723
Size: 394 354 3235
Fragments arranged by size:
3235 394 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 128 469 634 1566 1699 2158 2367 128
Size: 341 165 932 133 459 209 1744
Fragments arranged by size:
1744 932 459 341 209 165 133
TatI w'GTAC_w
Cuts at: 345 942 3498 345
Size: 597 2556 830
Fragments arranged by size:
2556 830 597
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3500 3532 3500
Size: 32 3951
Tth111I GACn'n_nGTC
Cuts at: 1780 1780
Size: 3983
XbaI T'CTAG_A
Cuts at: 1014 1014
Size: 3983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 228 1478 228
Size: 1250 2733
XmaI C'CCGG_G
Cuts at: 1003 1003
Size: 3983
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BspEI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspOMI PspXI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI