Restriction map for
pF1KB9538
(Circular) (Six-base) MAPSORT of: pF1KB9538.seq Check: 4579 from: 1 to: 4169
pF1KB9538 4169 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1207 1207
Size: 4169
Acc65I G'GTAC_C
Cuts at: 1185 1185
Size: 4169
AclI AA'CG_TT
Cuts at: 4009 4009
Size: 4169
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 1994 2426 3173 3441 394
Size: 1600 432 747 268 1122
Fragments arranged by size:
1600 1122 747 432 268
AflII C'TTAA_G
Cuts at: 160 160
Size: 4169
AflIII A'CryG_T
Cuts at: 1521 2625 3602 1521
Size: 1104 977 2088
Fragments arranged by size:
2088 1104 977
AgeI A'CCGG_T
Cuts at: 2557 2557
Size: 4169
AhdI GACnn_n'nnGTC
Cuts at: 1129 1129
Size: 4169
AlwNI CAG_nnn'CTG
Cuts at: 1474 3041 1474
Size: 1567 2602
ApaLI G'TGCA_C
Cuts at: 2939 2939
Size: 4169
ApoI r'AATT_y
Cuts at: 349 638 806 1173 3522 3778 349
Size: 289 168 367 2349 256 740
Fragments arranged by size:
2349 740 367 289 256 168
AseI AT'TA_AT
Cuts at: 20 20
Size: 4169
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
AvaI C'yCGr_G
Cuts at: 1189 1189
Size: 4169
BamHI G'GATC_C
Cuts at: 1194 1194
Size: 4169
BanI G'GyrC_C
Cuts at: 1185 1599 1847 1882 1185
Size: 414 248 35 3472
Fragments arranged by size:
3472 414 248 35
BanII G_rGCy'C
Cuts at: 1183 2213 1183
Size: 1030 3139
BbeI G_GCGC'C
Cuts at: 1603 1851 1603
Size: 248 3921
BbvCI CC'TCA_GC
Cuts at: 436 436
Size: 4169
BciVI GTATCCnnnnn_n'
Cuts at: 2067 2828 4169 2067
Size: 761 1341 2067
Fragments arranged by size:
2067 1341 761
BclI T'GATC_A
Cuts at: 1689 1689
Size: 4169
BglII A'GATC_T
Cuts at: 1684 3721 1684
Size: 2037 2132
BlpI GC'TnA_GC
Cuts at: 1281 1281
Size: 4169
Bme1580I G_kGCm'C
Cuts at: 1794 1887 2943 1794
Size: 93 1056 3020
Fragments arranged by size:
3020 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 656 1381 1462 1797 3941 656
Size: 725 81 335 2144 884
Fragments arranged by size:
2144 884 725 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 211 1055 2536 211
Size: 844 1481 1844
Fragments arranged by size:
1844 1481 844
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 584 584
Size: 4169
Bpu10I CC'TnA_GC
Cuts at: 436 436
Size: 4169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1345 2224 2716 3014 1345
Size: 879 492 298 2500
Fragments arranged by size:
2500 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 110 3873 110
Size: 3763 406
BsaAI yAC'GTr
Cuts at: 1522 1522
Size: 4169
BsaBI GATnn'nnATC
Cuts at: 6 1683 3720 6
Size: 1677 2037 455
Fragments arranged by size:
2037 1677 455
BsaHI Gr'CG_yC
Cuts at: 1600 1848 2550 4046 1600
Size: 248 702 1496 1723
Fragments arranged by size:
1723 1496 702 248
BsaWI w'CCGG_w
Cuts at: 669 1358 1879 2557 2831 2978 3389 669
Size: 689 521 678 274 147 411 1449
Fragments arranged by size:
1449 689 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 526 526
Size: 4169
BseYI C'CCAG_C
Cuts at: 1594 2352 2929 3425 1594
Size: 758 577 496 2338
Fragments arranged by size:
2338 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 771 939 771
Size: 168 4001
BsiEI CG_ry'CG
Cuts at: 104 1711 1757 2965 104
Size: 1607 46 1208 1308
Fragments arranged by size:
1607 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 726 1183 1961 2151 2943 726
Size: 457 778 190 792 1952
Fragments arranged by size:
1952 792 778 457 190
BsmI GAATG_Cn'
Cuts at: 586 586
Size: 4169
Bsp1286I G_dGCh'C
Cuts at: 726 1183 1794 1887 1961 2151 2213 2943 726
Size: 457 611 93 74 190 62 730 1952
Fragments arranged by size:
1952 730 611 457 190 93 74 62
BspEI T'CCGG_A
Cuts at: 669 3389 669
Size: 2720 1449
BspHI T'CATG_A
Cuts at: 3345 3345
Size: 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1219 1433 1735 2116 1219
Size: 214 302 381 3272
Fragments arranged by size:
3272 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2461 2515 3999 4164 50
Size: 2411 54 1484 165 55
Fragments arranged by size:
2411 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 591 2081 591
Size: 1490 2679
BsrFI r'CCGG_y
Cuts at: 2167 2348 2557 2167
Size: 181 209 3779
Fragments arranged by size:
3779 209 181
BsrGI T'GTAC_A
Cuts at: 711 711
Size: 4169
BssSI C'ACGA_G
Cuts at: 2440 2798 2440
Size: 358 3811
BstAPI GCAn_nnn'nTGC
Cuts at: 1546 1546
Size: 4169
BstBI TT'CG_AA
Cuts at: 2530 2530
Size: 4169
BstYI r'GATC_y
Cuts at: 1 1194 1661 1684 2019 2265 3277 3363 3375
Size: 1193 467 23 335 246 1012 86 12
Cuts at: 3375 3721 1
Size: 346 449
Fragments arranged by size:
1193 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4169
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2119 2300 2463 2119
Size: 181 163 3825
Fragments arranged by size:
3825 181 163
BtsI GCAGTG_nn'
Cuts at: 759 927 1412 759
Size: 168 485 3516
Fragments arranged by size:
3516 485 168
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 405 440 405
Size: 35 4134
DraI TTT'AAA
Cuts at: 459 1168 459
Size: 709 3460
DrdI GACnn_nn'nnGTC
Cuts at: 1875 2733 1875
Size: 858 3311
EaeI y'GGCC_r
Cuts at: 1754 1928 2319 2346 3691 1754
Size: 174 391 27 1345 2232
Fragments arranged by size:
2232 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1754 1754
Size: 4169
EarI CTCTTCn'nnn_
Cuts at: 2192 2402 2192
Size: 210 3959
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2377 2687 2833 3979 2377
Size: 310 146 1146 2567
Fragments arranged by size:
2567 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1181 1181
Size: 4169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 584 1994 2426 3173 3441 394
Size: 190 1410 432 747 268 1122
Fragments arranged by size:
1410 1122 747 432 268 190
EcoNI CCTnn'n_nnAGG
Cuts at: 1110 1110
Size: 4169
EcoO109I rG'GnC_Cy
Cuts at: 1308 1308
Size: 4169
EcoRI G'AATT_C
Cuts at: 638 806 1173 638
Size: 168 367 3634
Fragments arranged by size:
3634 367 168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 618 2188 618
Size: 1570 2599
FspI TGC'GCA
Cuts at: 1950 1950
Size: 4169
HaeII r_GCGC'y
Cuts at: 1603 1851 2873 3852 1603
Size: 248 1022 979 1920
Fragments arranged by size:
1920 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1115 1133 1147 1165 2011 2041 2043 2073 1115
Size: 18 14 18 846 30 2 30 3211
Fragments arranged by size:
3211 846 30 30 18 18 14 2
HincII GTy'rAC
Cuts at: 1037 1208 1037
Size: 171 3998
HindIII A'AGCT_T
Cuts at: 683 683
Size: 4169
KasI G'GCGC_C
Cuts at: 1599 1847 1599
Size: 248 3921
KpnI G_GTAC'C
Cuts at: 1189 1189
Size: 4169
MfeI C'AATT_G
Cuts at: 176 176
Size: 4169
MluI A'CGCG_T
Cuts at: 3602 3602
Size: 4169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2840 3024 2840
Size: 184 3985
MscI TGG'CCA
Cuts at: 1930 1930
Size: 4169
MslI CAynn'nnrTG
Cuts at: 579 967 1083 2158 2285 579
Size: 388 116 1075 127 2463
Fragments arranged by size:
2463 1075 388 127 116
MspA1I CmG'CkG
Cuts at: 1280 1594 1954 2967 3212 1280
Size: 314 360 1013 245 2237
Fragments arranged by size:
2237 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2350 2350
Size: 4169
NarI GG'CG_CC
Cuts at: 1600 1848 1600
Size: 248 3921
NcoI C'CATG_G
Cuts at: 107 107
Size: 4169
NdeI CA'TA_TG
Cuts at: 576 728 1080 576
Size: 152 352 3665
Fragments arranged by size:
3665 352 152
NgoMIV G'CCGG_C
Cuts at: 2348 2348
Size: 4169
NspI r_CATG'y
Cuts at: 1222 1722 2629 1222
Size: 500 907 2762
Fragments arranged by size:
2762 907 500
PciI A'CATG_T
Cuts at: 2625 2625
Size: 4169
PmeI GTTT'AAAC
Cuts at: 1168 1168
Size: 4169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 4137
PsiI TTA'TAA
Cuts at: 254 254
Size: 4169
PstI C_TGCA'G
Cuts at: 1216 1901 1216
Size: 685 3484
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4169
PvuII CAG'CTG
Cuts at: 1594 1954 1594
Size: 360 3809
RsrII CG'GwC_CG
Cuts at: 2364 2364
Size: 4169
SacI G_AGCT'C
Cuts at: 1183 1183
Size: 4169
SalI G'TCGA_C
Cuts at: 1206 1206
Size: 4169
SapI GCTCTTCn'nnn_
Cuts at: 2192 2402 2192
Size: 210 3959
SbfI CC_TGCA'GG
Cuts at: 1216 1216
Size: 4169
ScaI AGT'ACT
Cuts at: 3686 3686
Size: 4169
SfcI C'TryA_G
Cuts at: 33 1212 1897 2890 3081 33
Size: 1179 685 993 191 1121
Fragments arranged by size:
1179 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1601 1849 1601
Size: 248 3921
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4169
SmaI CCC'GGG
Cuts at: 1191 1191
Size: 4169
SmlI C'TyrA_G
Cuts at: 160 1324 2239 2731 2993 160
Size: 1164 915 492 262 1336
Fragments arranged by size:
1336 1164 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4169
SphI G_CATG'C
Cuts at: 1222 1722 1222
Size: 500 3669
SspI AAT'ATT
Cuts at: 406 406
Size: 4169
StuI AGG'CCT
Cuts at: 1105 1105
Size: 4169
StyI C'CwwG_G
Cuts at: 107 1303 1657 107
Size: 1196 354 2619
Fragments arranged by size:
2619 1196 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 706 1752 1885 2344 2553 706
Size: 1046 133 459 209 2322
Fragments arranged by size:
2322 1046 459 209 133
TatI w'GTAC_w
Cuts at: 499 711 1003 3684 499
Size: 212 292 2681 984
Fragments arranged by size:
2681 984 292 212
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3686 3718 3686
Size: 32 4137
Tth111I GACn'n_nGTC
Cuts at: 1966 1966
Size: 4169
XbaI T'CTAG_A
Cuts at: 1200 1200
Size: 4169
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1664 1664
Size: 4169
XmaI C'CCGG_G
Cuts at: 1189 1189
Size: 4169
XmnI GAAnn'nnTTC
Cuts at: 601 658 601
Size: 57 4112
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI DraIII EcoRV FseI FspAI HpaI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI