Restriction map for
pF1KB9537
(Circular) (Six-base) MAPSORT of: pF1KB9537.seq Check: 8807 from: 1 to: 4298
pF1KB9537 4298 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 296 767 296
Size: 471 3827
AccI GT'mk_AC
Cuts at: 572 1336 572
Size: 764 3534
Acc65I G'GTAC_C
Cuts at: 1314 1314
Size: 4298
AclI AA'CG_TT
Cuts at: 4138 4138
Size: 4298
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 612 2123 2555 3302 3570 612
Size: 1511 432 747 268 1340
Fragments arranged by size:
1511 1340 747 432 268
AflIII A'CryG_T
Cuts at: 917 1650 2754 3731 917
Size: 733 1104 977 1484
Fragments arranged by size:
1484 1104 977 733
AgeI A'CCGG_T
Cuts at: 2686 2686
Size: 4298
AleI CACnn'nnGTG
Cuts at: 1171 1171
Size: 4298
AlwNI CAG_nnn'CTG
Cuts at: 437 839 1111 1260 1603 3170 437
Size: 402 272 149 343 1567 1565
Fragments arranged by size:
1567 1565 402 343 272 149
ApaLI G'TGCA_C
Cuts at: 853 3068 853
Size: 2215 2083
ApoI r'AATT_y
Cuts at: 1302 3651 3907 1302
Size: 2349 256 1693
Fragments arranged by size:
2349 1693 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4298
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4298
AvaI C'yCGr_G
Cuts at: 120 1029 1318 120
Size: 909 289 3100
Fragments arranged by size:
3100 909 289
BamHI G'GATC_C
Cuts at: 1323 1323
Size: 4298
BanI G'GyrC_C
Cuts at: 151 842 995 1314 1728 1976 2011 151
Size: 691 153 319 414 248 35 2438
Fragments arranged by size:
2438 691 414 319 248 153 35
BanII G_rGCy'C
Cuts at: 1312 2342 1312
Size: 1030 3268
BbeI G_GCGC'C
Cuts at: 155 1732 1980 155
Size: 1577 248 2473
Fragments arranged by size:
2473 1577 248
BbvCI CC'TCA_GC
Cuts at: 1112 1112
Size: 4298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4264
BciVI GTATCCnnnnn_n'
Cuts at: 2196 2957 4298 2196
Size: 761 1341 2196
Fragments arranged by size:
2196 1341 761
BclI T'GATC_A
Cuts at: 1818 1818
Size: 4298
BglI GCCn_nnn'nGGC
Cuts at: 993 993
Size: 4298
BglII A'GATC_T
Cuts at: 1813 3850 1813
Size: 2037 2261
BlpI GC'TnA_GC
Cuts at: 1410 1410
Size: 4298
Bme1580I G_kGCm'C
Cuts at: 833 857 1923 2016 3072 833
Size: 24 1066 93 1056 2059
Fragments arranged by size:
2059 1066 1056 93 24
BmgBI CAC'GTC
Cuts at: 309 729 309
Size: 420 3878
BmrI ACTGGGnnnn_n'
Cuts at: 827 900 1285 1510 1591 1926 4070 827
Size: 73 385 225 81 335 2144 1055
Fragments arranged by size:
2144 1055 385 335 225 81 73
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2665 2665
Size: 4298
Bpu10I CC'TnA_GC
Cuts at: 1112 1112
Size: 4298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1048 1474 2353 2845 3143 1048
Size: 426 879 492 298 2203
Fragments arranged by size:
2203 879 492 426 298
BsaI GGTCTCn'nnnn_
Cuts at: 4002 4002
Size: 4298
BsaAI yAC'GTr
Cuts at: 1651 1651
Size: 4298
BsaBI GATnn'nnATC
Cuts at: 6 1812 3849 6
Size: 1806 2037 455
Fragments arranged by size:
2037 1806 455
BsaHI Gr'CG_yC
Cuts at: 152 259 1729 1977 2679 4175 152
Size: 107 1470 248 702 1496 275
Fragments arranged by size:
1496 1470 702 275 248 107
BsaWI w'CCGG_w
Cuts at: 477 1487 2008 2686 2960 3107 3518 477
Size: 1010 521 678 274 147 411 1257
Fragments arranged by size:
1257 1010 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 296 326 718 748 859 889 296
Size: 30 392 30 111 30 3705
Fragments arranged by size:
3705 392 111 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 414 658 973 414
Size: 244 315 3739
Fragments arranged by size:
3739 315 244
BseYI C'CCAG_C
Cuts at: 233 251 738 1123 1723 2481 3058 3554 233
Size: 18 487 385 600 758 577 496 977
Fragments arranged by size:
977 758 600 577 496 487 385 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 486 745 973 134
Size: 352 259 228 3459
Fragments arranged by size:
3459 352 259 228
BsiEI CG_ry'CG
Cuts at: 104 1840 1886 3094 104
Size: 1736 46 1208 1308
Fragments arranged by size:
1736 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 368 857 1312 2090 2280 3072 368
Size: 489 455 778 190 792 1594
Fragments arranged by size:
1594 792 778 489 455 190
BsmBI CGTCTCn'nnnn_
Cuts at: 144 299 144
Size: 155 4143
Bsp1286I G_dGCh'C
Cuts at: 368 833 857 1312 1923 2016 2090 2280 2342
Size: 465 24 455 611 93 74 190 62
Cuts at: 2342 3072 368
Size: 730 1594
Fragments arranged by size:
1594 730 611 465 455 190 93 74
62 24
BspEI T'CCGG_A
Cuts at: 3518 3518
Size: 4298
BspHI T'CATG_A
Cuts at: 1016 3474 1016
Size: 2458 1840
BspMI ACCTGCnnnn'nnnn_
Cuts at: 206 296 725 767 1348 1562 1864 2245 206
Size: 90 429 42 581 214 302 381 2259
Fragments arranged by size:
2259 581 429 381 302 214 90 42
BsrBI CCG'CTC
Cuts at: 50 623 2590 2644 4128 4293 50
Size: 573 1967 54 1484 165 55
Fragments arranged by size:
1967 1484 573 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2210 2210
Size: 4298
BsrFI r'CCGG_y
Cuts at: 242 2296 2477 2686 242
Size: 2054 181 209 1854
Fragments arranged by size:
2054 1854 209 181
BssSI C'ACGA_G
Cuts at: 2569 2927 2569
Size: 358 3940
BstAPI GCAn_nnn'nTGC
Cuts at: 1675 1675
Size: 4298
BstBI TT'CG_AA
Cuts at: 1136 2659 1136
Size: 1523 2775
BstXI CCAn_nnnn'nTGG
Cuts at: 781 781
Size: 4298
BstYI r'GATC_y
Cuts at: 1 1323 1790 1813 2148 2394 3406 3492 3504
Size: 1322 467 23 335 246 1012 86 12
Cuts at: 3504 3850 1
Size: 346 449
Fragments arranged by size:
1322 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1096 1096
Size: 4298
BtgI C'CryG_G
Cuts at: 263 708 766 263
Size: 445 58 3795
Fragments arranged by size:
3795 445 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1056 2248 2429 2592 1056
Size: 1192 181 163 2762
Fragments arranged by size:
2762 1192 181 163
BtsI GCAGTG_nn'
Cuts at: 1002 1541 1002
Size: 539 3759
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 630 665 630
Size: 35 4263
DraI TTT'AAA
Cuts at: 1297 1297
Size: 4298
DraIII CAC_nnn'GTG
Cuts at: 852 852
Size: 4298
DrdI GACnn_nn'nnGTC
Cuts at: 2004 2862 2004
Size: 858 3440
EaeI y'GGCC_r
Cuts at: 911 1883 2057 2448 2475 3820 911
Size: 972 174 391 27 1345 1389
Fragments arranged by size:
1389 1345 972 391 174 27
EagI C'GGCC_G
Cuts at: 1883 1883
Size: 4298
EarI CTCTTCn'nnn_
Cuts at: 676 683 1040 1075 2321 2531 676
Size: 7 357 35 1246 210 2443
Fragments arranged by size:
2443 1246 357 210 35 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2506 2816 2962 4108 2506
Size: 310 146 1146 2696
Fragments arranged by size:
2696 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1310 1310
Size: 4298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 612 2123 2555 3302 3570 612
Size: 1511 432 747 268 1340
Fragments arranged by size:
1511 1340 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 429 1099 429
Size: 670 3628
EcoO109I rG'GnC_Cy
Cuts at: 580 693 1022 1437 580
Size: 113 329 415 3441
Fragments arranged by size:
3441 415 329 113
EcoRI G'AATT_C
Cuts at: 1302 1302
Size: 4298
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 4298
FspI TGC'GCA
Cuts at: 1193 2079 1193
Size: 886 3412
HaeII r_GCGC'y
Cuts at: 155 221 252 927 1732 1980 3002 3981 155
Size: 66 31 675 805 248 1022 979 472
Fragments arranged by size:
1022 979 805 675 472 248 66 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 371 403 718 750 859 891 2140 2170 2172
Size: 32 315 32 109 32 1249 30 2
Cuts at: 2172 2202 371
Size: 30 2467
Fragments arranged by size:
2467 1249 315 109 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1337 1337
Size: 4298
KasI G'GCGC_C
Cuts at: 151 1728 1976 151
Size: 1577 248 2473
Fragments arranged by size:
2473 1577 248
KpnI G_GTAC'C
Cuts at: 1318 1318
Size: 4298
MluI A'CGCG_T
Cuts at: 3731 3731
Size: 4298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 542 2969 3153 542
Size: 2427 184 1687
Fragments arranged by size:
2427 1687 184
MscI TGG'CCA
Cuts at: 913 2059 913
Size: 1146 3152
MslI CAynn'nnrTG
Cuts at: 1171 2287 2414 1171
Size: 1116 127 3055
Fragments arranged by size:
3055 1116 127
MspA1I CmG'CkG
Cuts at: 265 593 632 1111 1260 1409 1723 2083 3096
Size: 328 39 479 149 149 314 360 1013
Cuts at: 3096 3341 265
Size: 245 1222
Fragments arranged by size:
1222 1013 479 360 328 314 245 149
149 39
NaeI GCC'GGC
Cuts at: 244 2479 244
Size: 2235 2063
NarI GG'CG_CC
Cuts at: 152 1729 1977 152
Size: 1577 248 2473
Fragments arranged by size:
2473 1577 248
NgoMIV G'CCGG_C
Cuts at: 242 2477 242
Size: 2235 2063
NspI r_CATG'y
Cuts at: 921 1351 1851 2758 921
Size: 430 500 907 2461
Fragments arranged by size:
2461 907 500 430
PasI CC'CwG_GG
Cuts at: 431 679 431
Size: 248 4050
PciI A'CATG_T
Cuts at: 917 2754 917
Size: 1837 2461
PflMI CCAn_nnn'nTGG
Cuts at: 839 920 1212 839
Size: 81 292 3925
Fragments arranged by size:
3925 292 81
PfoI T'CCnGG_A
Cuts at: 338 338
Size: 4298
PmeI GTTT'AAAC
Cuts at: 1297 1297
Size: 4298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3437 3469 3437
Size: 32 4266
PpuMI rG'GwC_Cy
Cuts at: 1022 1022
Size: 4298
PstI C_TGCA'G
Cuts at: 1256 1345 2030 1256
Size: 89 685 3524
Fragments arranged by size:
3524 685 89
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4298
PvuII CAG'CTG
Cuts at: 593 632 1260 1723 2083 593
Size: 39 628 463 360 2808
Fragments arranged by size:
2808 628 463 360 39
RsrII CG'GwC_CG
Cuts at: 2493 2493
Size: 4298
SacI G_AGCT'C
Cuts at: 1312 1312
Size: 4298
SacII CC_GC'GG
Cuts at: 266 266
Size: 4298
SalI G'TCGA_C
Cuts at: 1335 1335
Size: 4298
SapI GCTCTTCn'nnn_
Cuts at: 2321 2531 2321
Size: 210 4088
SbfI CC_TGCA'GG
Cuts at: 1345 1345
Size: 4298
ScaI AGT'ACT
Cuts at: 3815 3815
Size: 4298
SexAI A'CCwGG_T
Cuts at: 848 848
Size: 4298
SfcI C'TryA_G
Cuts at: 33 1252 1341 2026 3019 3210 33
Size: 1219 89 685 993 191 1121
Fragments arranged by size:
1219 1121 993 685 191 89
SfoI GGC'GCC
Cuts at: 153 1730 1978 153
Size: 1577 248 2473
Fragments arranged by size:
2473 1577 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4298
SmaI CCC'GGG
Cuts at: 122 1320 122
Size: 1198 3100
SmlI C'TyrA_G
Cuts at: 1063 1453 2368 2860 3122 1063
Size: 390 915 492 262 2239
Fragments arranged by size:
2239 915 492 390 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4298
SphI G_CATG'C
Cuts at: 1351 1851 1351
Size: 500 3798
SrfI GCCC'GGGC
Cuts at: 122 122
Size: 4298
StyI C'CwwG_G
Cuts at: 791 1184 1432 1786 791
Size: 393 248 354 3303
Fragments arranged by size:
3303 393 354 248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 243 779 1881 2014 2473 2682 243
Size: 536 1102 133 459 209 1859
Fragments arranged by size:
1859 1102 536 459 209 133
TatI w'GTAC_w
Cuts at: 404 506 3813 404
Size: 102 3307 889
Fragments arranged by size:
3307 889 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3815 3847 3815
Size: 32 4266
Tth111I GACn'n_nGTC
Cuts at: 2095 2095
Size: 4298
XbaI T'CTAG_A
Cuts at: 1329 1329
Size: 4298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1793 1793
Size: 4298
XmaI C'CCGG_G
Cuts at: 120 1318 120
Size: 1198 3100
XmnI GAAnn'nnTTC
Cuts at: 532 532
Size: 4298
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BfrBI BmtI BpmI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I ClaI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspOMI PspXI PsrI
SanDI SfiI SgrAI SnaBI SspI StuI SwaI XhoI
ZraI