Restriction map for
pF1KB9536
(Circular) (Six-base) MAPSORT of: pF1KB9536.seq Check: 3318 from: 1 to: 4319
pF1KB9536 4319 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1357 1357
Size: 4319
Acc65I G'GTAC_C
Cuts at: 1335 1335
Size: 4319
AclI AA'CG_TT
Cuts at: 4159 4159
Size: 4319
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 582 2144 2576 3323 3591 101
Size: 481 1562 432 747 268 829
Fragments arranged by size:
1562 829 747 481 432 268
AfeI AGC'GCT
Cuts at: 446 446
Size: 4319
AflIII A'CryG_T
Cuts at: 490 1671 2775 3752 490
Size: 1181 1104 977 1057
Fragments arranged by size:
1181 1104 1057 977
AgeI A'CCGG_T
Cuts at: 2707 2707
Size: 4319
AleI CACnn'nnGTG
Cuts at: 641 893 1071 1229 641
Size: 252 178 158 3731
Fragments arranged by size:
3731 252 178 158
AlwNI CAG_nnn'CTG
Cuts at: 1624 3191 1624
Size: 1567 2752
ApaLI G'TGCA_C
Cuts at: 874 3089 874
Size: 2215 2104
ApoI r'AATT_y
Cuts at: 481 510 1046 1323 3672 3928 481
Size: 29 536 277 2349 256 872
Fragments arranged by size:
2349 872 536 277 256 29
AseI AT'TA_AT
Cuts at: 20 20
Size: 4319
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
AvaI C'yCGr_G
Cuts at: 664 1339 664
Size: 675 3644
AvrII C'CTAG_G
Cuts at: 1101 1101
Size: 4319
BamHI G'GATC_C
Cuts at: 380 1344 380
Size: 964 3355
BanI G'GyrC_C
Cuts at: 832 959 1335 1749 1997 2032 832
Size: 127 376 414 248 35 3119
Fragments arranged by size:
3119 414 376 248 127 35
BanII G_rGCy'C
Cuts at: 1333 2363 1333
Size: 1030 3289
BbeI G_GCGC'C
Cuts at: 1753 2001 1753
Size: 248 4071
BciVI GTATCCnnnnn_n'
Cuts at: 2217 2978 4319 2217
Size: 761 1341 2217
Fragments arranged by size:
2217 1341 761
BclI T'GATC_A
Cuts at: 1839 1839
Size: 4319
BfrBI ATG'CAT
Cuts at: 204 204
Size: 4319
BglI GCCn_nnn'nGGC
Cuts at: 557 557
Size: 4319
BglII A'GATC_T
Cuts at: 360 696 1026 1834 3871 360
Size: 336 330 808 2037 808
Fragments arranged by size:
2037 808 808 336 330
BlpI GC'TnA_GC
Cuts at: 1276 1431 1276
Size: 155 4164
Bme1580I G_kGCm'C
Cuts at: 878 1944 2037 3093 878
Size: 1066 93 1056 2104
Fragments arranged by size:
2104 1066 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 314 734 818 1531 1612 1947 4091 314
Size: 420 84 713 81 335 2144 542
Fragments arranged by size:
2144 713 542 420 335 84 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2686 2686
Size: 4319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1291 1495 2374 2866 3164 1291
Size: 204 879 492 298 2446
Fragments arranged by size:
2446 879 492 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 4023 4023
Size: 4319
BsaAI yAC'GTr
Cuts at: 1672 1672
Size: 4319
BsaBI GATnn'nnATC
Cuts at: 6 1833 3870 6
Size: 1827 2037 455
Fragments arranged by size:
2037 1827 455
BsaHI Gr'CG_yC
Cuts at: 1750 1998 2700 4196 1750
Size: 248 702 1496 1873
Fragments arranged by size:
1873 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1508 2029 2707 2981 3128 3539 1508
Size: 521 678 274 147 411 2288
Fragments arranged by size:
2288 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1162 1162
Size: 4319
BseYI C'CCAG_C
Cuts at: 1744 2502 3079 3575 1744
Size: 758 577 496 2488
Fragments arranged by size:
2488 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1861 1907 3115 104
Size: 1757 46 1208 1308
Fragments arranged by size:
1757 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 878 1333 2111 2301 3093 878
Size: 455 778 190 792 2104
Fragments arranged by size:
2104 792 778 455 190
BsmI GAATG_Cn'
Cuts at: 253 253
Size: 4319
Bsp1286I G_dGCh'C
Cuts at: 878 1333 1944 2037 2111 2301 2363 3093 878
Size: 455 611 93 74 190 62 730 2104
Fragments arranged by size:
2104 730 611 455 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3539 3539
Size: 4319
BspHI T'CATG_A
Cuts at: 3495 3495
Size: 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 463 1369 1583 1885 2266 463
Size: 906 214 302 381 2516
Fragments arranged by size:
2516 906 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2611 2665 4149 4314 50
Size: 2561 54 1484 165 55
Fragments arranged by size:
2561 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2231 2231
Size: 4319
BsrFI r'CCGG_y
Cuts at: 2317 2498 2707 2317
Size: 181 209 3929
Fragments arranged by size:
3929 209 181
BsrGI T'GTAC_A
Cuts at: 110 789 1035 110
Size: 679 246 3394
Fragments arranged by size:
3394 679 246
BssSI C'ACGA_G
Cuts at: 2590 2948 2590
Size: 358 3961
BstAPI GCAn_nnn'nTGC
Cuts at: 1696 1696
Size: 4319
BstBI TT'CG_AA
Cuts at: 2680 2680
Size: 4319
BstXI CCAn_nnnn'nTGG
Cuts at: 1073 1073
Size: 4319
BstYI r'GATC_y
Cuts at: 1 360 380 696 1026 1344 1811 1834 2169
Size: 359 20 316 330 318 467 23 335
Cuts at: 2169 2415 3427 3513 3525 3871 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 359 346 335 330 318
316 246 86 23 20 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2269 2450 2613 2269
Size: 181 163 3975
Fragments arranged by size:
3975 181 163
BtsI GCAGTG_nn'
Cuts at: 1562 1562
Size: 4319
DraI TTT'AAA
Cuts at: 987 1318 987
Size: 331 3988
DrdI GACnn_nn'nnGTC
Cuts at: 2025 2883 2025
Size: 858 3461
EaeI y'GGCC_r
Cuts at: 852 1904 2078 2469 2496 3841 852
Size: 1052 174 391 27 1345 1330
Fragments arranged by size:
1345 1330 1052 391 174 27
EagI C'GGCC_G
Cuts at: 1904 1904
Size: 4319
EarI CTCTTCn'nnn_
Cuts at: 116 418 1052 2342 2552 116
Size: 302 634 1290 210 1883
Fragments arranged by size:
1883 1290 634 302 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2527 2837 2983 4129 2527
Size: 310 146 1146 2717
Fragments arranged by size:
2717 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1331 1331
Size: 4319
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 582 2144 2576 3323 3591 101
Size: 481 1562 432 747 268 829
Fragments arranged by size:
1562 829 747 481 432 268
EcoO109I rG'GnC_Cy
Cuts at: 734 1458 734
Size: 724 3595
EcoRI G'AATT_C
Cuts at: 1046 1323 1046
Size: 277 4042
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 276 2338 276
Size: 2062 2257
FspI TGC'GCA
Cuts at: 2100 2100
Size: 4319
HaeII r_GCGC'y
Cuts at: 448 1753 2001 3023 4002 448
Size: 1305 248 1022 979 765
Fragments arranged by size:
1305 1022 979 765 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 210 242 513 545 2161 2191 2193 2223 210
Size: 32 271 32 1616 30 2 30 2306
Fragments arranged by size:
2306 1616 271 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1358 1358
Size: 4319
HindIII A'AGCT_T
Cuts at: 677 1008 1182 677
Size: 331 174 3814
Fragments arranged by size:
3814 331 174
KasI G'GCGC_C
Cuts at: 1749 1997 1749
Size: 248 4071
KpnI G_GTAC'C
Cuts at: 1339 1339
Size: 4319
MluI A'CGCG_T
Cuts at: 3752 3752
Size: 4319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2990 3174 2990
Size: 184 4135
MscI TGG'CCA
Cuts at: 854 2080 854
Size: 1226 3093
MslI CAynn'nnrTG
Cuts at: 203 573 641 657 893 1071 1229 2308 2435
Size: 370 68 16 236 178 158 1079 127
Cuts at: 2435 203
Size: 2087
Fragments arranged by size:
2087 1079 370 236 178 158 127 68
16
MspA1I CmG'CkG
Cuts at: 1430 1744 2104 3117 3362 1430
Size: 314 360 1013 245 2387
Fragments arranged by size:
2387 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2500 2500
Size: 4319
NarI GG'CG_CC
Cuts at: 1750 1998 1750
Size: 248 4071
NdeI CA'TA_TG
Cuts at: 200 200
Size: 4319
NgoMIV G'CCGG_C
Cuts at: 2498 2498
Size: 4319
NsiI A_TGCA'T
Cuts at: 206 206
Size: 4319
NspI r_CATG'y
Cuts at: 472 494 1372 1872 2779 472
Size: 22 878 500 907 2012
Fragments arranged by size:
2012 907 878 500 22
PciI A'CATG_T
Cuts at: 490 2775 490
Size: 2285 2034
PmeI GTTT'AAAC
Cuts at: 1318 1318
Size: 4319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3458 3490 3458
Size: 32 4287
PsiI TTA'TAA
Cuts at: 320 404 320
Size: 84 4235
PstI C_TGCA'G
Cuts at: 1366 2051 1366
Size: 685 3634
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4319
PvuII CAG'CTG
Cuts at: 1744 2104 1744
Size: 360 3959
RsrII CG'GwC_CG
Cuts at: 2514 2514
Size: 4319
SacI G_AGCT'C
Cuts at: 1333 1333
Size: 4319
SalI G'TCGA_C
Cuts at: 1356 1356
Size: 4319
SapI GCTCTTCn'nnn_
Cuts at: 418 2342 2552 418
Size: 1924 210 2185
Fragments arranged by size:
2185 1924 210
SbfI CC_TGCA'GG
Cuts at: 1366 1366
Size: 4319
ScaI AGT'ACT
Cuts at: 3836 3836
Size: 4319
SfcI C'TryA_G
Cuts at: 33 1017 1362 2047 3040 3231 33
Size: 984 345 685 993 191 1121
Fragments arranged by size:
1121 993 984 685 345 191
SfoI GGC'GCC
Cuts at: 1751 1999 1751
Size: 248 4071
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
SmaI CCC'GGG
Cuts at: 1341 1341
Size: 4319
SmlI C'TyrA_G
Cuts at: 664 1270 1474 2389 2881 3143 664
Size: 606 204 915 492 262 1840
Fragments arranged by size:
1840 915 606 492 262 204
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4319
SphI G_CATG'C
Cuts at: 1372 1872 1372
Size: 500 3819
StyI C'CwwG_G
Cuts at: 1101 1453 1807 1101
Size: 352 354 3613
Fragments arranged by size:
3613 354 352
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1902 2035 2494 2703 1902
Size: 133 459 209 3518
Fragments arranged by size:
3518 459 209 133
TatI w'GTAC_w
Cuts at: 110 607 789 1035 1221 3834 110
Size: 497 182 246 186 2613 595
Fragments arranged by size:
2613 595 497 246 186 182
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3836 3868 3836
Size: 32 4287
Tth111I GACn'n_nGTC
Cuts at: 2116 2116
Size: 4319
XbaI T'CTAG_A
Cuts at: 1350 1350
Size: 4319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1814 1814
Size: 4319
XhoI C'TCGA_G
Cuts at: 664 664
Size: 4319
XmaI C'CCGG_G
Cuts at: 1339 1339
Size: 4319
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NsiI NspI PciI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
BaeI BbsI BbvCI BcgI BmgBI BmtI BpmI Bpu10I
BsaXI BsgI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI