Restriction map for
pF1KB9522
(Circular) (Six-base) MAPSORT of: pF1KB9522.seq Check: 6841 from: 1 to: 5174
pF1KB9522 5174 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1561 1561
Size: 5174
AccI GT'mk_AC
Cuts at: 2212 2212
Size: 5174
Acc65I G'GTAC_C
Cuts at: 2190 2190
Size: 5174
AclI AA'CG_TT
Cuts at: 5014 5014
Size: 5174
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 697 969 1806 2125 2999 3431 4178 4446 697
Size: 272 837 319 874 432 747 268 1425
Fragments arranged by size:
1425 874 837 747 432 319 272 268
AflIII A'CryG_T
Cuts at: 632 1911 2526 3630 4607 632
Size: 1279 615 1104 977 1199
Fragments arranged by size:
1279 1199 1104 977 615
AgeI A'CCGG_T
Cuts at: 3562 3562
Size: 5174
AhdI GACnn_n'nnGTC
Cuts at: 783 783
Size: 5174
AleI CACnn'nnGTG
Cuts at: 1864 1864
Size: 5174
AlwNI CAG_nnn'CTG
Cuts at: 123 675 741 852 1501 1878 2479 4046 123
Size: 552 66 111 649 377 601 1567 1251
Fragments arranged by size:
1567 1251 649 601 552 377 111 66
ApaI G_GGCC'C
Cuts at: 505 505
Size: 5174
ApaLI G'TGCA_C
Cuts at: 1300 1423 3944 1300
Size: 123 2521 2530
Fragments arranged by size:
2530 2521 123
ApoI r'AATT_y
Cuts at: 1014 2178 4527 4783 1014
Size: 1164 2349 256 1405
Fragments arranged by size:
2349 1405 1164 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5174
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5174
AvaI C'yCGr_G
Cuts at: 1179 1403 1573 2084 2142 2194 1179
Size: 224 170 511 58 52 4159
Fragments arranged by size:
4159 511 224 170 58 52
BamHI G'GATC_C
Cuts at: 1778 2199 1778
Size: 421 4753
BanI G'GyrC_C
Cuts at: 172 1615 1987 2190 2604 2852 2887 172
Size: 1443 372 203 414 248 35 2459
Fragments arranged by size:
2459 1443 414 372 248 203 35
BanII G_rGCy'C
Cuts at: 505 544 644 1091 2188 3218 505
Size: 39 100 447 1097 1030 2461
Fragments arranged by size:
2461 1097 1030 447 100 39
BbeI G_GCGC'C
Cuts at: 2608 2856 2608
Size: 248 4926
BbsI GAAGACnn'nnnn_
Cuts at: 203 1166 1440 203
Size: 963 274 3937
Fragments arranged by size:
3937 963 274
BbvCI CC'TCA_GC
Cuts at: 1933 1933
Size: 5174
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 261 295 1452 1486 261
Size: 34 1157 34 3949
Fragments arranged by size:
3949 1157 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3072 3833 5174 3072
Size: 761 1341 3072
Fragments arranged by size:
3072 1341 761
BclI T'GATC_A
Cuts at: 2694 2694
Size: 5174
BglII A'GATC_T
Cuts at: 2689 4726 2689
Size: 2037 3137
BlpI GC'TnA_GC
Cuts at: 664 1474 1645 2286 664
Size: 810 171 641 3552
Fragments arranged by size:
3552 810 641 171
Bme1580I G_kGCm'C
Cuts at: 177 505 1304 1427 2799 2892 3948 177
Size: 328 799 123 1372 93 1056 1403
Fragments arranged by size:
1403 1372 1056 799 328 123 93
BmrI ACTGGGnnnn_n'
Cuts at: 111 557 2386 2467 2802 4946 111
Size: 446 1829 81 335 2144 339
Fragments arranged by size:
2144 1829 446 339 335 81
BmtI G_CTAG'C
Cuts at: 1858 1858
Size: 5174
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 512 1418 3541 512
Size: 906 2123 2145
Fragments arranged by size:
2145 2123 906
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 763 811 829 1374 1730 640
Size: 123 48 18 545 356 4084
Fragments arranged by size:
4084 545 356 123 48 18
Bpu10I CC'TnA_GC
Cuts at: 497 1315 1933 497
Size: 818 618 3738
Fragments arranged by size:
3738 818 618
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 681 1624 2350 3229 3721 4019 681
Size: 943 726 879 492 298 1836
Fragments arranged by size:
1836 943 879 726 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1201 1281 4878 1201
Size: 80 3597 1497
Fragments arranged by size:
3597 1497 80
BsaAI yAC'GTr
Cuts at: 1332 2527 1332
Size: 1195 3979
BsaBI GATnn'nnATC
Cuts at: 6 2688 4725 6
Size: 2682 2037 455
Fragments arranged by size:
2682 2037 455
BsaHI Gr'CG_yC
Cuts at: 1295 1754 2605 2853 3555 5051 1295
Size: 459 851 248 702 1496 1418
Fragments arranged by size:
1496 1418 851 702 459 248
BsaWI w'CCGG_w
Cuts at: 800 2363 2884 3562 3836 3983 4394 800
Size: 1563 521 678 274 147 411 1580
Fragments arranged by size:
1580 1563 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 847 877 1372 1402 847
Size: 30 495 30 4619
Fragments arranged by size:
4619 495 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 454 499 808 889 892 961 1269 1312 454
Size: 45 309 81 3 69 308 43 4316
Fragments arranged by size:
4316 309 308 81 69 45 43 3
BseYI C'CCAG_C
Cuts at: 400 1198 1658 1926 2599 3357 3934 4430 400
Size: 798 460 268 673 758 577 496 1144
Fragments arranged by size:
1144 798 758 673 577 496 460 268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 838 1519 1891 838
Size: 681 372 4121
Fragments arranged by size:
4121 681 372
BsiEI CG_ry'CG
Cuts at: 104 611 2716 2762 3970 104
Size: 507 2105 46 1208 1308
Fragments arranged by size:
2105 1308 1208 507 46
BsiHKAI G_wGCw'C
Cuts at: 644 1100 1304 1427 2188 2966 3156 3948 644
Size: 456 204 123 761 778 190 792 1870
Fragments arranged by size:
1870 792 778 761 456 204 190 123
BsmBI CGTCTCn'nnnn_
Cuts at: 1350 1350
Size: 5174
Bsp1286I G_dGCh'C
Cuts at: 177 505 544 644 1091 1100 1304 1427 2188
Size: 328 39 100 447 9 204 123 761
Cuts at: 2188 2799 2892 2966 3156 3218 3948 177
Size: 611 93 74 190 62 730 1403
Fragments arranged by size:
1403 761 730 611 447 328 204 190
123 100 93 74 62 39 9
BspEI T'CCGG_A
Cuts at: 4394 4394
Size: 5174
BspHI T'CATG_A
Cuts at: 4350 4350
Size: 5174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 275 860 1442 1561 2224 2438 2740 3121 275
Size: 585 582 119 663 214 302 381 2328
Fragments arranged by size:
2328 663 585 582 381 302 214 119
BsrBI CCG'CTC
Cuts at: 50 613 3466 3520 5004 5169 50
Size: 563 2853 54 1484 165 55
Fragments arranged by size:
2853 1484 563 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1147 1370 3086 1147
Size: 223 1716 3235
Fragments arranged by size:
3235 1716 223
BsrFI r'CCGG_y
Cuts at: 838 1482 3172 3353 3562 838
Size: 644 1690 181 209 2450
Fragments arranged by size:
2450 1690 644 209 181
BssSI C'ACGA_G
Cuts at: 1461 1597 3445 3803 1461
Size: 136 1848 358 2832
Fragments arranged by size:
2832 1848 358 136
BstAPI GCAn_nnn'nTGC
Cuts at: 1878 2551 1878
Size: 673 4501
BstBI TT'CG_AA
Cuts at: 3535 3535
Size: 5174
BstXI CCAn_nnnn'nTGG
Cuts at: 1677 1728 1866 1677
Size: 51 138 4985
Fragments arranged by size:
4985 138 51
BstYI r'GATC_y
Cuts at: 1 1778 1874 2199 2666 2689 3024 3270 4282
Size: 1777 96 325 467 23 335 246 1012
Cuts at: 4282 4368 4380 4726 1
Size: 86 12 346 449
Fragments arranged by size:
1777 1012 467 449 346 335 325 246
96 86 23 12
BtgI C'CryG_G
Cuts at: 177 812 1091 1652 1862 177
Size: 635 279 561 210 3489
Fragments arranged by size:
3489 635 561 279 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3124 3305 3468 3124
Size: 181 163 4830
Fragments arranged by size:
4830 181 163
BtsI GCAGTG_nn'
Cuts at: 1200 1271 1921 2417 1200
Size: 71 650 496 3957
Fragments arranged by size:
3957 650 496 71
DraI TTT'AAA
Cuts at: 2173 2173
Size: 5174
DraIII CAC_nnn'GTG
Cuts at: 818 818
Size: 5174
DrdI GACnn_nn'nnGTC
Cuts at: 117 1792 2880 3738 117
Size: 1675 1088 858 1553
Fragments arranged by size:
1675 1553 1088 858
EaeI y'GGCC_r
Cuts at: 608 1442 1847 2759 2933 3324 3351 4696 608
Size: 834 405 912 174 391 27 1345 1086
Fragments arranged by size:
1345 1086 912 834 405 391 174 27
EagI C'GGCC_G
Cuts at: 608 2759 608
Size: 2151 3023
EarI CTCTTCn'nnn_
Cuts at: 163 287 931 958 1825 2101 3197 3407 163
Size: 124 644 27 867 276 1096 210 1930
Fragments arranged by size:
1930 1096 867 644 276 210 124 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3382 3692 3838 4984 3382
Size: 310 146 1146 3572
Fragments arranged by size:
3572 1146 310 146
EcoICRI GAG'CTC
Cuts at: 642 2186 642
Size: 1544 3630
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 697 763 811 829 969 1374 1730 1806
Size: 57 66 48 18 140 405 356 76
Cuts at: 1806 2125 2999 3431 4178 4446 640
Size: 319 874 432 747 268 1368
Fragments arranged by size:
1368 874 747 432 405 356 319 268
140 76 66 57 48 18
EcoNI CCTnn'n_nnAGG
Cuts at: 496 699 1003 496
Size: 203 304 4667
Fragments arranged by size:
4667 304 203
EcoO109I rG'GnC_Cy
Cuts at: 297 363 501 502 651 704 849 2313 297
Size: 66 138 1 149 53 145 1464 3158
Fragments arranged by size:
3158 1464 149 145 138 66 53 1
EcoRI G'AATT_C
Cuts at: 1014 2178 1014
Size: 1164 4010
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 450 3193 450
Size: 2743 2431
FspI TGC'GCA
Cuts at: 271 2955 271
Size: 2684 2490
HaeII r_GCGC'y
Cuts at: 1652 2126 2608 2856 3878 4857 1652
Size: 474 482 248 1022 979 1969
Fragments arranged by size:
1969 1022 979 482 474 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 396 428 439 471 769 801 3016 3046 3048
Size: 32 11 32 298 32 2215 30 2
Cuts at: 3048 3078 396
Size: 30 2492
Fragments arranged by size:
2492 2215 298 32 32 32 30 30
11 2
HincII GTy'rAC
Cuts at: 2213 2213
Size: 5174
KasI G'GCGC_C
Cuts at: 2604 2852 2604
Size: 248 4926
KpnI G_GTAC'C
Cuts at: 2194 2194
Size: 5174
MluI A'CGCG_T
Cuts at: 4607 4607
Size: 5174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3845 4029 3845
Size: 184 4990
MscI TGG'CCA
Cuts at: 1849 2935 1849
Size: 1086 4088
MslI CAynn'nnrTG
Cuts at: 1420 1849 1864 1867 3163 3290 1420
Size: 429 15 3 1296 127 3304
Fragments arranged by size:
3304 1296 429 127 15 3
MspA1I CmG'CkG
Cuts at: 219 510 660 2285 2599 2959 3972 4217 219
Size: 291 150 1625 314 360 1013 245 1176
Fragments arranged by size:
1625 1176 1013 360 314 291 245 150
NaeI GCC'GGC
Cuts at: 840 3355 840
Size: 2515 2659
NarI GG'CG_CC
Cuts at: 2605 2853 2605
Size: 248 4926
NcoI C'CATG_G
Cuts at: 1652 1862 1652
Size: 210 4964
NgoMIV G'CCGG_C
Cuts at: 838 3353 838
Size: 2515 2659
NheI G'CTAG_C
Cuts at: 1854 1854
Size: 5174
NspI r_CATG'y
Cuts at: 400 636 1915 2227 2727 3634 400
Size: 236 1279 312 500 907 1940
Fragments arranged by size:
1940 1279 907 500 312 236
PasI CC'CwG_GG
Cuts at: 359 1955 359
Size: 1596 3578
PciI A'CATG_T
Cuts at: 632 1911 3630 632
Size: 1279 1719 2176
Fragments arranged by size:
2176 1719 1279
PflMI CCAn_nnn'nTGG
Cuts at: 818 1029 1658 1775 818
Size: 211 629 117 4217
Fragments arranged by size:
4217 629 211 117
PfoI T'CCnGG_A
Cuts at: 845 1004 1390 1561 1781 845
Size: 159 386 171 220 4238
Fragments arranged by size:
4238 386 220 171 159
PmeI GTTT'AAAC
Cuts at: 2173 2173
Size: 5174
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4313 4345 4313
Size: 32 5142
PpuMI rG'GwC_Cy
Cuts at: 651 849 651
Size: 198 4976
PspOMI G'GGCC_C
Cuts at: 501 501
Size: 5174
PspXI vC'TCGA_Gb
Cuts at: 1573 1573
Size: 5174
PstI C_TGCA'G
Cuts at: 131 214 327 2081 2221 2906 131
Size: 83 113 1754 140 685 2399
Fragments arranged by size:
2399 1754 685 140 113 83
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5174
PvuII CAG'CTG
Cuts at: 660 2599 2959 660
Size: 1939 360 2875
Fragments arranged by size:
2875 1939 360
RsrII CG'GwC_CG
Cuts at: 3369 3369
Size: 5174
SacI G_AGCT'C
Cuts at: 644 2188 644
Size: 1544 3630
SalI G'TCGA_C
Cuts at: 2211 2211
Size: 5174
SapI GCTCTTCn'nnn_
Cuts at: 163 2101 3197 3407 163
Size: 1938 1096 210 1930
Fragments arranged by size:
1938 1930 1096 210
SbfI CC_TGCA'GG
Cuts at: 2221 2221
Size: 5174
ScaI AGT'ACT
Cuts at: 901 4691 901
Size: 3790 1384
SfcI C'TryA_G
Cuts at: 33 127 210 323 1117 2077 2217 2902 3895
Size: 94 83 113 794 960 140 685 993
Cuts at: 3895 4086 33
Size: 191 1121
Fragments arranged by size:
1121 993 960 794 685 191 140 113
94 83
SfoI GGC'GCC
Cuts at: 2606 2854 2606
Size: 248 4926
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5174
SmaI CCC'GGG
Cuts at: 1181 2086 2196 1181
Size: 905 110 4159
Fragments arranged by size:
4159 905 110
SmlI C'TyrA_G
Cuts at: 696 1573 1639 2329 3244 3736 3998 696
Size: 877 66 690 915 492 262 1872
Fragments arranged by size:
1872 915 877 690 492 262 66
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5174
SphI G_CATG'C
Cuts at: 2227 2727 2227
Size: 500 4674
StuI AGG'CCT
Cuts at: 694 694
Size: 5174
StyI C'CwwG_G
Cuts at: 168 1652 1862 1944 2037 2308 2662 168
Size: 1484 210 82 93 271 354 2680
Fragments arranged by size:
2680 1484 354 271 210 93 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1021 1190 1912 2757 2890 3349 3558 1021
Size: 169 722 845 133 459 209 2637
Fragments arranged by size:
2637 845 722 459 209 169 133
TatI w'GTAC_w
Cuts at: 899 4689 899
Size: 3790 1384
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 322 354 847 879 4691 4723 322
Size: 32 493 32 3812 32 773
Fragments arranged by size:
3812 773 493 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2971 2971
Size: 5174
XbaI T'CTAG_A
Cuts at: 2205 2205
Size: 5174
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1026 2669 1026
Size: 1643 3531
XhoI C'TCGA_G
Cuts at: 1573 1573
Size: 5174
XmaI C'CCGG_G
Cuts at: 1179 2084 2194 1179
Size: 905 110 4159
Fragments arranged by size:
4159 905 110
XmnI GAAnn'nnTTC
Cuts at: 200 200
Size: 5174
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BfrBI BglI BmgBI BsiWI BsmI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NotI NruI NsiI PacI PmlI
PshAI PsiI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI ZraI