Restriction map for
pF1KB9517
(Circular) (Six-base) MAPSORT of: pF1KB9517.seq Check: 8215 from: 1 to: 4808
pF1KB9517 4808 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1344 1344
Size: 4808
AccI GT'mk_AC
Cuts at: 1846 1846
Size: 4808
Acc65I G'GTAC_C
Cuts at: 1824 1824
Size: 4808
AclI AA'CG_TT
Cuts at: 4648 4648
Size: 4808
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 1480 1702 2633 3065 3812 4080 266
Size: 1214 222 931 432 747 268 994
Fragments arranged by size:
1214 994 931 747 432 268 222
AflIII A'CryG_T
Cuts at: 179 863 2160 3264 4241 179
Size: 684 1297 1104 977 746
Fragments arranged by size:
1297 1104 977 746 684
AgeI A'CCGG_T
Cuts at: 1085 3196 1085
Size: 2111 2697
AhdI GACnn_n'nnGTC
Cuts at: 1234 1234
Size: 4808
AleI CACnn'nnGTG
Cuts at: 589 1595 589
Size: 1006 3802
AlwNI CAG_nnn'CTG
Cuts at: 396 1144 1640 1653 2113 3680 396
Size: 748 496 13 460 1567 1524
Fragments arranged by size:
1567 1524 748 496 460 13
ApaI G_GGCC'C
Cuts at: 174 1512 1568 174
Size: 1338 56 3414
Fragments arranged by size:
3414 1338 56
ApaLI G'TGCA_C
Cuts at: 867 1750 3578 867
Size: 883 1828 2097
Fragments arranged by size:
2097 1828 883
ApoI r'AATT_y
Cuts at: 1015 1812 4161 4417 1015
Size: 797 2349 256 1406
Fragments arranged by size:
2349 1406 797 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4808
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4808
AvaI C'yCGr_G
Cuts at: 329 742 1828 329
Size: 413 1086 3309
Fragments arranged by size:
3309 1086 413
BamHI G'GATC_C
Cuts at: 1044 1833 1044
Size: 789 4019
BanI G'GyrC_C
Cuts at: 596 650 738 1824 2238 2486 2521 596
Size: 54 88 1086 414 248 35 2883
Fragments arranged by size:
2883 1086 414 248 88 54 35
BanII G_rGCy'C
Cuts at: 174 254 1512 1568 1822 2852 174
Size: 80 1258 56 254 1030 2130
Fragments arranged by size:
2130 1258 1030 254 80 56
BbeI G_GCGC'C
Cuts at: 654 2242 2490 654
Size: 1588 248 2972
Fragments arranged by size:
2972 1588 248
BbsI GAAGACnn'nnnn_
Cuts at: 1501 1690 1501
Size: 189 4619
BciVI GTATCCnnnnn_n'
Cuts at: 2706 3467 4808 2706
Size: 761 1341 2706
Fragments arranged by size:
2706 1341 761
BclI T'GATC_A
Cuts at: 1298 2328 1298
Size: 1030 3778
BglII A'GATC_T
Cuts at: 2323 4360 2323
Size: 2037 2771
BlpI GC'TnA_GC
Cuts at: 943 1920 943
Size: 977 3831
Bme1580I G_kGCm'C
Cuts at: 174 743 871 1512 1568 1578 1754 2433 2526
Size: 569 128 641 56 10 176 679 93
Cuts at: 2526 3582 174
Size: 1056 1400
Fragments arranged by size:
1400 1056 679 641 569 176 128 93
56 10
BmgBI CAC'GTC
Cuts at: 527 1758 527
Size: 1231 3577
BmrI ACTGGGnnnn_n'
Cuts at: 533 2020 2101 2436 4580 533
Size: 1487 81 335 2144 761
Fragments arranged by size:
2144 1487 761 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3175 3175
Size: 4808
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 238
Size: 4808
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1417 1984 2863 3355 3653 1417
Size: 567 879 492 298 2572
Fragments arranged by size:
2572 879 567 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 211 4512 211
Size: 4301 507
BsaAI yAC'GTr
Cuts at: 916 1691 2161 916
Size: 775 470 3563
Fragments arranged by size:
3563 775 470
BsaBI GATnn'nnATC
Cuts at: 6 772 2322 4359 6
Size: 766 1550 2037 455
Fragments arranged by size:
2037 1550 766 455
BsaHI Gr'CG_yC
Cuts at: 651 2239 2487 3189 4685 651
Size: 1588 248 702 1496 774
Fragments arranged by size:
1588 1496 774 702 248
BsaWI w'CCGG_w
Cuts at: 1085 1997 2518 3196 3470 3617 4028 1085
Size: 912 521 678 274 147 411 1865
Fragments arranged by size:
1865 912 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 191 221 451 481 1195 1225 191
Size: 30 230 30 714 30 3774
Fragments arranged by size:
3774 714 230 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 278 459 715 846 901 934 1216 1327 1363
Size: 181 256 131 55 33 282 111 36
Cuts at: 1363 1462 1558 278
Size: 99 96 3528
Fragments arranged by size:
3528 282 256 181 131 111 99 96
55 36 33
BseYI C'CCAG_C
Cuts at: 212 337 1553 2233 2991 3568 4064 212
Size: 125 1216 680 758 577 496 956
Fragments arranged by size:
1216 956 758 680 577 496 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1405 1507 1619 1405
Size: 102 112 4594
Fragments arranged by size:
4594 112 102
BsiEI CG_ry'CG
Cuts at: 104 1247 2350 2396 3604 104
Size: 1143 1103 46 1208 1308
Fragments arranged by size:
1308 1208 1143 1103 46
BsiHKAI G_wGCw'C
Cuts at: 416 871 1280 1754 1822 2600 2790 3582 416
Size: 455 409 474 68 778 190 792 1642
Fragments arranged by size:
1642 792 778 474 455 409 190 68
BsiWI C'GTAC_G
Cuts at: 1361 1361
Size: 4808
BsmBI CGTCTCn'nnnn_
Cuts at: 471 471
Size: 4808
Bsp1286I G_dGCh'C
Cuts at: 174 254 416 743 871 1280 1512 1568 1578
Size: 80 162 327 128 409 232 56 10
Cuts at: 1578 1754 1822 2433 2526 2600 2790 2852 3582
Size: 176 68 611 93 74 190 62 730
Cuts at: 3582 174
Size: 1400
Fragments arranged by size:
1400 730 611 409 327 232 190 176
162 128 93 80 74 68 62 56
10
BspEI T'CCGG_A
Cuts at: 4028 4028
Size: 4808
BspHI T'CATG_A
Cuts at: 978 1295 1760 3984 978
Size: 317 465 2224 1802
Fragments arranged by size:
2224 1802 465 317
BspMI ACCTGCnnnn'nnnn_
Cuts at: 947 1344 1858 2072 2374 2755 947
Size: 397 514 214 302 381 3000
Fragments arranged by size:
3000 514 397 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3100 3154 4638 4803 50
Size: 3050 54 1484 165 55
Fragments arranged by size:
3050 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1625 2720 1625
Size: 1095 3713
BsrFI r'CCGG_y
Cuts at: 1085 1412 1615 2806 2987 3196 1085
Size: 327 203 1191 181 209 2697
Fragments arranged by size:
2697 1191 327 209 203 181
BsrGI T'GTAC_A
Cuts at: 1211 1211
Size: 4808
BssSI C'ACGA_G
Cuts at: 3079 3437 3079
Size: 358 4450
BstAPI GCAn_nnn'nTGC
Cuts at: 371 1530 1643 2185 371
Size: 1159 113 542 2994
Fragments arranged by size:
2994 1159 542 113
BstBI TT'CG_AA
Cuts at: 3169 3169
Size: 4808
BstYI r'GATC_y
Cuts at: 1 306 1044 1833 2300 2323 2658 2904 3916
Size: 305 738 789 467 23 335 246 1012
Cuts at: 3916 4002 4014 4360 1
Size: 86 12 346 449
Fragments arranged by size:
1012 789 738 467 449 346 335 305
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 604 641 782 604
Size: 37 141 4630
Fragments arranged by size:
4630 141 37
BtgI C'CryG_G
Cuts at: 503 563 815 503
Size: 60 252 4496
Fragments arranged by size:
4496 252 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1200 2758 2939 3102 1200
Size: 1558 181 163 2906
Fragments arranged by size:
2906 1558 181 163
BtsI GCAGTG_nn'
Cuts at: 195 631 1590 1602 2051 195
Size: 436 959 12 449 2952
Fragments arranged by size:
2952 959 449 436 12
DraI TTT'AAA
Cuts at: 1807 1807
Size: 4808
DraIII CAC_nnn'GTG
Cuts at: 1530 1530
Size: 4808
DrdI GACnn_nn'nnGTC
Cuts at: 2514 3372 2514
Size: 858 3950
EaeI y'GGCC_r
Cuts at: 432 1342 2393 2567 2958 2985 4330 432
Size: 910 1051 174 391 27 1345 910
Fragments arranged by size:
1345 1051 910 910 391 174 27
EagI C'GGCC_G
Cuts at: 2393 2393
Size: 4808
EarI CTCTTCn'nnn_
Cuts at: 965 2831 3041 965
Size: 1866 210 2732
Fragments arranged by size:
2732 1866 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 915 1027 3016 3326 3472 4618 915
Size: 112 1989 310 146 1146 1105
Fragments arranged by size:
1989 1146 1105 310 146 112
EcoICRI GAG'CTC
Cuts at: 1820 1820
Size: 4808
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 266 1480 1702 2633 3065 3812 4080 238
Size: 28 1214 222 931 432 747 268 966
Fragments arranged by size:
1214 966 931 747 432 268 222 28
EcoNI CCTnn'n_nnAGG
Cuts at: 1006 1006
Size: 4808
EcoO109I rG'GnC_Cy
Cuts at: 170 171 987 1228 1509 1564 1565 1947 170
Size: 1 816 241 281 55 1 382 3031
Fragments arranged by size:
3031 816 382 281 241 55 1 1
EcoRI G'AATT_C
Cuts at: 1015 1812 1015
Size: 797 4011
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2827 2827
Size: 4808
FspI TGC'GCA
Cuts at: 2589 2589
Size: 4808
HaeII r_GCGC'y
Cuts at: 338 654 950 2242 2490 3512 4491 338
Size: 316 296 1292 248 1022 979 655
Fragments arranged by size:
1292 1022 979 655 316 296 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 781 813 1220 1252 2650 2680 2682 2712 781
Size: 32 407 32 1398 30 2 30 2877
Fragments arranged by size:
2877 1398 407 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1847 1847
Size: 4808
KasI G'GCGC_C
Cuts at: 650 2238 2486 650
Size: 1588 248 2972
Fragments arranged by size:
2972 1588 248
KpnI G_GTAC'C
Cuts at: 1828 1828
Size: 4808
MluI A'CGCG_T
Cuts at: 4241 4241
Size: 4808
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3479 3663 3479
Size: 184 4624
MscI TGG'CCA
Cuts at: 434 2569 434
Size: 2135 2673
MslI CAynn'nnrTG
Cuts at: 589 1294 1595 2797 2924 589
Size: 705 301 1202 127 2473
Fragments arranged by size:
2473 1202 705 301 127
MspA1I CmG'CkG
Cuts at: 425 495 1653 1782 1919 2233 2593 3606 3851
Size: 70 1158 129 137 314 360 1013 245
Cuts at: 3851 425
Size: 1382
Fragments arranged by size:
1382 1158 1013 360 314 245 137 129
70
NaeI GCC'GGC
Cuts at: 2989 2989
Size: 4808
NarI GG'CG_CC
Cuts at: 651 2239 2487 651
Size: 1588 248 2972
Fragments arranged by size:
2972 1588 248
NcoI C'CATG_G
Cuts at: 815 815
Size: 4808
NgoMIV G'CCGG_C
Cuts at: 2987 2987
Size: 4808
NspI r_CATG'y
Cuts at: 183 867 1733 1861 2361 3268 183
Size: 684 866 128 500 907 1723
Fragments arranged by size:
1723 907 866 684 500 128
PasI CC'CwG_GG
Cuts at: 1066 1066
Size: 4808
PciI A'CATG_T
Cuts at: 179 863 3264 179
Size: 684 2401 1723
Fragments arranged by size:
2401 1723 684
PflMI CCAn_nnn'nTGG
Cuts at: 509 509
Size: 4808
PfoI T'CCnGG_A
Cuts at: 254 722 254
Size: 468 4340
PmeI GTTT'AAAC
Cuts at: 1807 1807
Size: 4808
PmlI CAC'GTG
Cuts at: 916 1691 916
Size: 775 4033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 822 854 3947 3979 822
Size: 32 3093 32 1651
Fragments arranged by size:
3093 1651 32 32
PpuMI rG'GwC_Cy
Cuts at: 1228 1228
Size: 4808
PspOMI G'GGCC_C
Cuts at: 170 1508 1564 170
Size: 1338 56 3414
Fragments arranged by size:
3414 1338 56
PspXI vC'TCGA_Gb
Cuts at: 329 329
Size: 4808
PstI C_TGCA'G
Cuts at: 190 827 1010 1597 1855 2540 190
Size: 637 183 587 258 685 2458
Fragments arranged by size:
2458 685 637 587 258 183
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4808
PvuII CAG'CTG
Cuts at: 495 1653 1782 2233 2593 495
Size: 1158 129 451 360 2710
Fragments arranged by size:
2710 1158 451 360 129
RsrII CG'GwC_CG
Cuts at: 3003 3003
Size: 4808
SacI G_AGCT'C
Cuts at: 1822 1822
Size: 4808
SalI G'TCGA_C
Cuts at: 1845 1845
Size: 4808
SanDI GG'GwC_CC
Cuts at: 1228 1228
Size: 4808
SapI GCTCTTCn'nnn_
Cuts at: 965 2831 3041 965
Size: 1866 210 2732
Fragments arranged by size:
2732 1866 210
SbfI CC_TGCA'GG
Cuts at: 1855 1855
Size: 4808
ScaI AGT'ACT
Cuts at: 4325 4325
Size: 4808
SexAI A'CCwGG_T
Cuts at: 1526 1526
Size: 4808
SfcI C'TryA_G
Cuts at: 33 186 823 1006 1593 1851 2536 3529 3720
Size: 153 637 183 587 258 685 993 191
Cuts at: 3720 33
Size: 1121
Fragments arranged by size:
1121 993 685 637 587 258 191 183
153
SfoI GGC'GCC
Cuts at: 652 2240 2488 652
Size: 1588 248 2972
Fragments arranged by size:
2972 1588 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4808
SmaI CCC'GGG
Cuts at: 1830 1830
Size: 4808
SmlI C'TyrA_G
Cuts at: 329 1432 1963 2878 3370 3632 329
Size: 1103 531 915 492 262 1505
Fragments arranged by size:
1505 1103 915 531 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4808
SphI G_CATG'C
Cuts at: 1861 2361 1861
Size: 500 4308
StuI AGG'CCT
Cuts at: 1457 1457
Size: 4808
StyI C'CwwG_G
Cuts at: 815 1942 2296 815
Size: 1127 354 3327
Fragments arranged by size:
3327 1127 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 639 2391 2524 2983 3192 639
Size: 1752 133 459 209 2255
Fragments arranged by size:
2255 1752 459 209 133
TatI w'GTAC_w
Cuts at: 1211 4323 1211
Size: 3112 1696
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4325 4357 4325
Size: 32 4776
Tth111I GACn'n_nGTC
Cuts at: 2605 2605
Size: 4808
XbaI T'CTAG_A
Cuts at: 1839 1839
Size: 4808
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 546 1038 2303 546
Size: 492 1265 3051
Fragments arranged by size:
3051 1265 492
XhoI C'TCGA_G
Cuts at: 329 329
Size: 4808
XmaI C'CCGG_G
Cuts at: 1828 1828
Size: 4808
XmnI GAAnn'nnTTC
Cuts at: 974 974
Size: 4808
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmtI Bpu10I BsmI BssHII
BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PshAI PsiI PsrI SacII SfiI SgrAI SnaBI
SrfI SspI SwaI ZraI