Restriction map for
pF1KB9515
(Circular) (Six-base) MAPSORT of: pF1KB9515.seq Check: 6861 from: 1 to: 4646
pF1KB9515 4646 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 780 780
Size: 4646
AccI GT'mk_AC
Cuts at: 142 567 1049 1684 142
Size: 425 482 635 3104
Fragments arranged by size:
3104 635 482 425
Acc65I G'GTAC_C
Cuts at: 968 1662 968
Size: 694 3952
AclI AA'CG_TT
Cuts at: 4486 4486
Size: 4646
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 465 2471 2903 3650 3918 465
Size: 2006 432 747 268 1193
Fragments arranged by size:
2006 1193 747 432 268
AfeI AGC'GCT
Cuts at: 1446 1446
Size: 4646
AflIII A'CryG_T
Cuts at: 624 1998 3102 4079 624
Size: 1374 1104 977 1191
Fragments arranged by size:
1374 1191 1104 977
AgeI A'CCGG_T
Cuts at: 3034 3034
Size: 4646
AlwNI CAG_nnn'CTG
Cuts at: 364 693 1951 3518 364
Size: 329 1258 1567 1492
Fragments arranged by size:
1567 1492 1258 329
ApaLI G'TGCA_C
Cuts at: 3416 3416
Size: 4646
ApoI r'AATT_y
Cuts at: 336 518 907 1140 1650 3999 4255 336
Size: 182 389 233 510 2349 256 727
Fragments arranged by size:
2349 727 510 389 256 233 182
AseI AT'TA_AT
Cuts at: 20 1514 20
Size: 1494 3152
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4646
AvaI C'yCGr_G
Cuts at: 1666 1666
Size: 4646
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 468 501 468
Size: 33 4613
BamHI G'GATC_C
Cuts at: 1671 1671
Size: 4646
BanI G'GyrC_C
Cuts at: 968 1662 2076 2324 2359 968
Size: 694 414 248 35 3255
Fragments arranged by size:
3255 694 414 248 35
BanII G_rGCy'C
Cuts at: 119 948 1660 2690 119
Size: 829 712 1030 2075
Fragments arranged by size:
2075 1030 829 712
BbeI G_GCGC'C
Cuts at: 2080 2328 2080
Size: 248 4398
BbsI GAAGACnn'nnnn_
Cuts at: 841 841
Size: 4646
BciVI GTATCCnnnnn_n'
Cuts at: 2544 3305 4646 2544
Size: 761 1341 2544
Fragments arranged by size:
2544 1341 761
BclI T'GATC_A
Cuts at: 2166 2166
Size: 4646
BglII A'GATC_T
Cuts at: 2161 4198 2161
Size: 2037 2609
BlpI GC'TnA_GC
Cuts at: 1758 1758
Size: 4646
Bme1580I G_kGCm'C
Cuts at: 2271 2364 3420 2271
Size: 93 1056 3497
Fragments arranged by size:
3497 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1858 1939 2274 4418 1858
Size: 81 335 2144 2086
Fragments arranged by size:
2144 2086 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3013 3013
Size: 4646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 701 1442 701
Size: 741 3905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1822 2701 3193 3491 1822
Size: 879 492 298 2977
Fragments arranged by size:
2977 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4350 4350
Size: 4646
BsaAI yAC'GTr
Cuts at: 1999 1999
Size: 4646
BsaBI GATnn'nnATC
Cuts at: 6 2160 4197 6
Size: 2154 2037 455
Fragments arranged by size:
2154 2037 455
BsaHI Gr'CG_yC
Cuts at: 1556 2077 2325 3027 4523 1556
Size: 521 248 702 1496 1679
Fragments arranged by size:
1679 1496 702 521 248
BsaWI w'CCGG_w
Cuts at: 1835 2356 3034 3308 3455 3866 1835
Size: 521 678 274 147 411 2615
Fragments arranged by size:
2615 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1552 1582 1552
Size: 30 4616
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1109 1109
Size: 4646
BseYI C'CCAG_C
Cuts at: 1405 2071 2829 3406 3902 1405
Size: 666 758 577 496 2149
Fragments arranged by size:
2149 758 666 577 496
BsiEI CG_ry'CG
Cuts at: 104 149 2188 2234 3442 104
Size: 45 2039 46 1208 1308
Fragments arranged by size:
2039 1308 1208 46 45
BsiHKAI G_wGCw'C
Cuts at: 1346 1660 2438 2628 3420 1346
Size: 314 778 190 792 2572
Fragments arranged by size:
2572 792 778 314 190
Bsp1286I G_dGCh'C
Cuts at: 119 948 1346 1660 2271 2364 2438 2628 2690
Size: 829 398 314 611 93 74 190 62
Cuts at: 2690 3420 119
Size: 730 1345
Fragments arranged by size:
1345 829 730 611 398 314 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3866 3866
Size: 4646
BspHI T'CATG_A
Cuts at: 558 3822 558
Size: 3264 1382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 780 1696 1910 2212 2593 780
Size: 916 214 302 381 2833
Fragments arranged by size:
2833 916 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2938 2992 4476 4641 50
Size: 2888 54 1484 165 55
Fragments arranged by size:
2888 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1620 2558 1620
Size: 938 3708
BsrFI r'CCGG_y
Cuts at: 188 2644 2825 3034 188
Size: 2456 181 209 1800
Fragments arranged by size:
2456 1800 209 181
BsrGI T'GTAC_A
Cuts at: 977 977
Size: 4646
BssSI C'ACGA_G
Cuts at: 230 2917 3275 230
Size: 2687 358 1601
Fragments arranged by size:
2687 1601 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1273 1437 2023 1273
Size: 164 586 3896
Fragments arranged by size:
3896 586 164
BstBI TT'CG_AA
Cuts at: 3007 3007
Size: 4646
BstYI r'GATC_y
Cuts at: 1 424 1304 1671 2138 2161 2496 2742 3754
Size: 423 880 367 467 23 335 246 1012
Cuts at: 3754 3840 3852 4198 1
Size: 86 12 346 449
Fragments arranged by size:
1012 880 467 449 423 367 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1050 1050
Size: 4646
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2596 2777 2940 2596
Size: 181 163 4302
Fragments arranged by size:
4302 181 163
BtsI GCAGTG_nn'
Cuts at: 1889 1889
Size: 4646
DraI TTT'AAA
Cuts at: 594 1645 594
Size: 1051 3595
DrdI GACnn_nn'nnGTC
Cuts at: 2352 3210 2352
Size: 858 3788
EaeI y'GGCC_r
Cuts at: 1325 2231 2405 2796 2823 4168 1325
Size: 906 174 391 27 1345 1803
Fragments arranged by size:
1803 1345 906 391 174 27
EagI C'GGCC_G
Cuts at: 2231 2231
Size: 4646
EarI CTCTTCn'nnn_
Cuts at: 272 454 1346 2669 2879 272
Size: 182 892 1323 210 2039
Fragments arranged by size:
2039 1323 892 210 182
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2854 3164 3310 4456 2854
Size: 310 146 1146 3044
Fragments arranged by size:
3044 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1658 1658
Size: 4646
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 465 701 1442 2471 2903 3650 3918 465
Size: 236 741 1029 432 747 268 1193
Fragments arranged by size:
1193 1029 747 741 432 268 236
EcoO109I rG'GnC_Cy
Cuts at: 1785 1785
Size: 4646
EcoRI G'AATT_C
Cuts at: 907 1650 907
Size: 743 3903
EcoRV GAT'ATC
Cuts at: 240 741 795 240
Size: 501 54 4091
Fragments arranged by size:
4091 501 54
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2665 2665
Size: 4646
FspI TGC'GCA
Cuts at: 2427 2427
Size: 4646
HaeII r_GCGC'y
Cuts at: 1448 2080 2328 3350 4329 1448
Size: 632 248 1022 979 1765
Fragments arranged by size:
1765 1022 979 632 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 298 330 1515 1547 2488 2518 2520 2550 298
Size: 32 1185 32 941 30 2 30 2394
Fragments arranged by size:
2394 1185 941 32 32 30 30 2
HincII GTy'rAC
Cuts at: 143 468 1685 143
Size: 325 1217 3104
Fragments arranged by size:
3104 1217 325
KasI G'GCGC_C
Cuts at: 2076 2324 2076
Size: 248 4398
KpnI G_GTAC'C
Cuts at: 972 1666 972
Size: 694 3952
MfeI C'AATT_G
Cuts at: 125 125
Size: 4646
MluI A'CGCG_T
Cuts at: 624 4079 624
Size: 3455 1191
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3317 3501 3317
Size: 184 4462
MscI TGG'CCA
Cuts at: 1327 2407 1327
Size: 1080 3566
MslI CAynn'nnrTG
Cuts at: 2635 2762 2635
Size: 127 4519
MspA1I CmG'CkG
Cuts at: 1273 1409 1757 2071 2431 3444 3689 1273
Size: 136 348 314 360 1013 245 2230
Fragments arranged by size:
2230 1013 360 348 314 245 136
NaeI GCC'GGC
Cuts at: 2827 2827
Size: 4646
NarI GG'CG_CC
Cuts at: 2077 2325 2077
Size: 248 4398
NdeI CA'TA_TG
Cuts at: 777 777
Size: 4646
NgoMIV G'CCGG_C
Cuts at: 2825 2825
Size: 4646
NspI r_CATG'y
Cuts at: 978 1401 1699 2199 3106 978
Size: 423 298 500 907 2518
Fragments arranged by size:
2518 907 500 423 298
PciI A'CATG_T
Cuts at: 3102 3102
Size: 4646
PflMI CCAn_nnn'nTGG
Cuts at: 1177 1177
Size: 4646
PmeI GTTT'AAAC
Cuts at: 1645 1645
Size: 4646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 3785
Size: 32 4614
PstI C_TGCA'G
Cuts at: 1068 1278 1693 2378 1068
Size: 210 415 685 3336
Fragments arranged by size:
3336 685 415 210
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4646
PvuII CAG'CTG
Cuts at: 1273 2071 2431 1273
Size: 798 360 3488
Fragments arranged by size:
3488 798 360
RsrII CG'GwC_CG
Cuts at: 2841 2841
Size: 4646
SacI G_AGCT'C
Cuts at: 1660 1660
Size: 4646
SalI G'TCGA_C
Cuts at: 141 1683 141
Size: 1542 3104
SapI GCTCTTCn'nnn_
Cuts at: 2669 2879 2669
Size: 210 4436
SbfI CC_TGCA'GG
Cuts at: 1693 1693
Size: 4646
ScaI AGT'ACT
Cuts at: 1415 4163 1415
Size: 2748 1898
SfcI C'TryA_G
Cuts at: 33 885 1064 1274 1689 2374 3367 3558 33
Size: 852 179 210 415 685 993 191 1121
Fragments arranged by size:
1121 993 852 685 415 210 191 179
SfoI GGC'GCC
Cuts at: 2078 2326 2078
Size: 248 4398
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4646
SmaI CCC'GGG
Cuts at: 1668 1668
Size: 4646
SmlI C'TyrA_G
Cuts at: 1801 2716 3208 3470 1801
Size: 915 492 262 2977
Fragments arranged by size:
2977 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4646
SphI G_CATG'C
Cuts at: 1401 1699 2199 1401
Size: 298 500 3848
Fragments arranged by size:
3848 500 298
SspI AAT'ATT
Cuts at: 1635 1635
Size: 4646
StyI C'CwwG_G
Cuts at: 119 1496 1780 2134 119
Size: 1377 284 354 2631
Fragments arranged by size:
2631 1377 354 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 163 770 1523 2229 2362 2821 3030 163
Size: 607 753 706 133 459 209 1779
Fragments arranged by size:
1779 753 706 607 459 209 133
TatI w'GTAC_w
Cuts at: 977 1413 4161 977
Size: 436 2748 1462
Fragments arranged by size:
2748 1462 436
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4163 4195 4163
Size: 32 4614
Tth111I GACn'n_nGTC
Cuts at: 2443 2443
Size: 4646
XbaI T'CTAG_A
Cuts at: 1677 1677
Size: 4646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2141 2141
Size: 4646
XmaI C'CCGG_G
Cuts at: 1666 1666
Size: 4646
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BstZ17I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI Bsu36I
BtgI ClaI CspCI DraIII EcoNI FseI FspAI HindIII
HpaI NcoI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI