Restriction map for
pF1KB9513
(Circular) (Six-base) MAPSORT of: pF1KB9513.seq Check: 7445 from: 1 to: 4553
pF1KB9513 4553 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 205 1591 205
Size: 1386 3167
Acc65I G'GTAC_C
Cuts at: 407 1569 407
Size: 1162 3391
AclI AA'CG_TT
Cuts at: 4393 4393
Size: 4553
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2378 2810 3557 3825 2378
Size: 432 747 268 3106
Fragments arranged by size:
3106 747 432 268
AflIII A'CryG_T
Cuts at: 512 1484 1905 3009 3986 512
Size: 972 421 1104 977 1079
Fragments arranged by size:
1104 1079 977 972 421
AgeI A'CCGG_T
Cuts at: 2941 2941
Size: 4553
AleI CACnn'nnGTG
Cuts at: 460 460
Size: 4553
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1346 1378 1346
Size: 32 4521
AlwNI CAG_nnn'CTG
Cuts at: 645 867 1858 3425 645
Size: 222 991 1567 1773
Fragments arranged by size:
1773 1567 991 222
ApaLI G'TGCA_C
Cuts at: 3323 3323
Size: 4553
ApoI r'AATT_y
Cuts at: 1557 3906 4162 1557
Size: 2349 256 1948
Fragments arranged by size:
2349 1948 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4553
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
AvaI C'yCGr_G
Cuts at: 1573 1573
Size: 4553
BamHI G'GATC_C
Cuts at: 523 1578 523
Size: 1055 3498
BanI G'GyrC_C
Cuts at: 407 806 1150 1569 1983 2231 2266 407
Size: 399 344 419 414 248 35 2694
Fragments arranged by size:
2694 419 414 399 344 248 35
BanII G_rGCy'C
Cuts at: 272 602 1220 1567 2597 272
Size: 330 618 347 1030 2228
Fragments arranged by size:
2228 1030 618 347 330
BbeI G_GCGC'C
Cuts at: 1987 2235 1987
Size: 248 4305
BbsI GAAGACnn'nnnn_
Cuts at: 931 931
Size: 4553
BbvCI CC'TCA_GC
Cuts at: 172 646 172
Size: 474 4079
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4519
BciVI GTATCCnnnnn_n'
Cuts at: 2451 3212 4553 2451
Size: 761 1341 2451
Fragments arranged by size:
2451 1341 761
BclI T'GATC_A
Cuts at: 2073 2073
Size: 4553
BfrBI ATG'CAT
Cuts at: 1528 1528
Size: 4553
BglII A'GATC_T
Cuts at: 2068 4105 2068
Size: 2037 2516
BlpI GC'TnA_GC
Cuts at: 1665 1665
Size: 4553
Bme1580I G_kGCm'C
Cuts at: 1155 1450 2178 2271 3327 1155
Size: 295 728 93 1056 2381
Fragments arranged by size:
2381 1056 728 295 93
BmrI ACTGGGnnnn_n'
Cuts at: 731 1343 1765 1846 2181 4325 731
Size: 612 422 81 335 2144 959
Fragments arranged by size:
2144 959 612 422 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 4553
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 940
Size: 4553
Bpu10I CC'TnA_GC
Cuts at: 172 646 172
Size: 474 4079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1729 2608 3100 3398 1729
Size: 879 492 298 2884
Fragments arranged by size:
2884 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 378 709 4257 378
Size: 331 3548 674
Fragments arranged by size:
3548 674 331
BsaAI yAC'GTr
Cuts at: 1906 1906
Size: 4553
BsaBI GATnn'nnATC
Cuts at: 6 656 2067 4104 6
Size: 650 1411 2037 455
Fragments arranged by size:
2037 1411 650 455
BsaHI Gr'CG_yC
Cuts at: 1382 1984 2232 2934 4430 1382
Size: 602 248 702 1496 1505
Fragments arranged by size:
1505 1496 702 602 248
BsaWI w'CCGG_w
Cuts at: 526 1364 1742 2263 2941 3215 3362 3773 526
Size: 838 378 521 678 274 147 411 1306
Fragments arranged by size:
1306 838 678 521 411 378 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 659 689 659
Size: 30 4523
BseYI C'CCAG_C
Cuts at: 319 1978 2736 3313 3809 319
Size: 1659 758 577 496 1063
Fragments arranged by size:
1659 1063 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 438 640 438
Size: 202 4351
BsiEI CG_ry'CG
Cuts at: 104 1182 2095 2141 3349 104
Size: 1078 913 46 1208 1308
Fragments arranged by size:
1308 1208 1078 913 46
BsiHKAI G_wGCw'C
Cuts at: 602 1067 1220 1567 2345 2535 3327 602
Size: 465 153 347 778 190 792 1828
Fragments arranged by size:
1828 792 778 465 347 190 153
Bsp1286I G_dGCh'C
Cuts at: 272 602 1067 1155 1220 1450 1567 2178 2271
Size: 330 465 88 65 230 117 611 93
Cuts at: 2271 2345 2535 2597 3327 272
Size: 74 190 62 730 1498
Fragments arranged by size:
1498 730 611 465 330 230 190 117
93 88 74 65 62
BspEI T'CCGG_A
Cuts at: 526 1364 3773 526
Size: 838 2409 1306
Fragments arranged by size:
2409 1306 838
BspHI T'CATG_A
Cuts at: 1239 3729 1239
Size: 2490 2063
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1603 1817 2119 2500 1603
Size: 214 302 381 3656
Fragments arranged by size:
3656 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2845 2899 4383 4548 50
Size: 2795 54 1484 165 55
Fragments arranged by size:
2795 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2465 2465
Size: 4553
BsrFI r'CCGG_y
Cuts at: 2551 2732 2941 2551
Size: 181 209 4163
Fragments arranged by size:
4163 209 181
BssSI C'ACGA_G
Cuts at: 2824 3182 2824
Size: 358 4195
BstAPI GCAn_nnn'nTGC
Cuts at: 627 1930 627
Size: 1303 3250
BstBI TT'CG_AA
Cuts at: 539 2914 539
Size: 2375 2178
BstXI CCAn_nnnn'nTGG
Cuts at: 951 951
Size: 4553
BstYI r'GATC_y
Cuts at: 1 523 1578 2045 2068 2403 2649 3661 3747
Size: 522 1055 467 23 335 246 1012 86
Cuts at: 3747 3759 4105 1
Size: 12 346 449
Fragments arranged by size:
1055 1012 522 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 396 396
Size: 4553
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2503 2684 2847 2503
Size: 181 163 4209
Fragments arranged by size:
4209 181 163
BtsI GCAGTG_nn'
Cuts at: 1512 1796 1512
Size: 284 4269
DraI TTT'AAA
Cuts at: 1552 1552
Size: 4553
DrdI GACnn_nn'nnGTC
Cuts at: 2259 3117 2259
Size: 858 3695
EaeI y'GGCC_r
Cuts at: 326 941 1179 2138 2312 2703 2730 4075 326
Size: 615 238 959 174 391 27 1345 804
Fragments arranged by size:
1345 959 804 615 391 238 174 27
EagI C'GGCC_G
Cuts at: 1179 2138 1179
Size: 959 3594
EarI CTCTTCn'nnn_
Cuts at: 1208 2576 2786 1208
Size: 1368 210 2975
Fragments arranged by size:
2975 1368 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2761 3071 3217 4363 2761
Size: 310 146 1146 2951
Fragments arranged by size:
2951 1146 310 146
EcoICRI GAG'CTC
Cuts at: 600 1218 1565 600
Size: 618 347 3588
Fragments arranged by size:
3588 618 347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 2378 2810 3557 3825 940
Size: 1438 432 747 268 1668
Fragments arranged by size:
1668 1438 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1692 1692
Size: 4553
EcoRI G'AATT_C
Cuts at: 1557 1557
Size: 4553
EcoRV GAT'ATC
Cuts at: 654 654
Size: 4553
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 4553
FspI TGC'GCA
Cuts at: 2334 2334
Size: 4553
HaeII r_GCGC'y
Cuts at: 1987 2235 3257 4236 1987
Size: 248 1022 979 2304
Fragments arranged by size:
2304 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2395 2425 2427 2457 2395
Size: 30 2 30 4491
Fragments arranged by size:
4491 30 30 2
HincII GTy'rAC
Cuts at: 206 1056 1592 206
Size: 850 536 3167
Fragments arranged by size:
3167 850 536
HindIII A'AGCT_T
Cuts at: 497 497
Size: 4553
KasI G'GCGC_C
Cuts at: 1983 2231 1983
Size: 248 4305
KpnI G_GTAC'C
Cuts at: 411 1573 411
Size: 1162 3391
MluI A'CGCG_T
Cuts at: 3986 3986
Size: 4553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3224 3408 3224
Size: 184 4369
MscI TGG'CCA
Cuts at: 943 2314 943
Size: 1371 3182
MslI CAynn'nnrTG
Cuts at: 460 2542 2669 460
Size: 2082 127 2344
Fragments arranged by size:
2344 2082 127
MspA1I CmG'CkG
Cuts at: 642 918 1664 1978 2338 3351 3596 642
Size: 276 746 314 360 1013 245 1599
Fragments arranged by size:
1599 1013 746 360 314 276 245
NaeI GCC'GGC
Cuts at: 2734 2734
Size: 4553
NarI GG'CG_CC
Cuts at: 1984 2232 1984
Size: 248 4305
NgoMIV G'CCGG_C
Cuts at: 2732 2732
Size: 4553
NsiI A_TGCA'T
Cuts at: 1530 1530
Size: 4553
NspI r_CATG'y
Cuts at: 385 516 1488 1532 1606 2106 3013 385
Size: 131 972 44 74 500 907 1925
Fragments arranged by size:
1925 972 907 500 131 74 44
PasI CC'CwG_GG
Cuts at: 1497 1497
Size: 4553
PciI A'CATG_T
Cuts at: 512 1484 3009 512
Size: 972 1525 2056
Fragments arranged by size:
2056 1525 972
PflMI CCAn_nnn'nTGG
Cuts at: 689 867 689
Size: 178 4375
PmeI GTTT'AAAC
Cuts at: 1552 1552
Size: 4553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3692 3724 3692
Size: 32 4521
PstI C_TGCA'G
Cuts at: 182 699 1600 2285 182
Size: 517 901 685 2450
Fragments arranged by size:
2450 901 685 517
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4553
PvuII CAG'CTG
Cuts at: 642 918 1978 2338 642
Size: 276 1060 360 2857
Fragments arranged by size:
2857 1060 360 276
RsrII CG'GwC_CG
Cuts at: 2748 2748
Size: 4553
SacI G_AGCT'C
Cuts at: 602 1220 1567 602
Size: 618 347 3588
Fragments arranged by size:
3588 618 347
SalI G'TCGA_C
Cuts at: 204 1590 204
Size: 1386 3167
SapI GCTCTTCn'nnn_
Cuts at: 1208 2576 2786 1208
Size: 1368 210 2975
Fragments arranged by size:
2975 1368 210
SbfI CC_TGCA'GG
Cuts at: 1600 1600
Size: 4553
ScaI AGT'ACT
Cuts at: 4070 4070
Size: 4553
SfcI C'TryA_G
Cuts at: 33 178 695 1596 2281 3274 3465 33
Size: 145 517 901 685 993 191 1121
Fragments arranged by size:
1121 993 901 685 517 191 145
SfoI GGC'GCC
Cuts at: 1985 2233 1985
Size: 248 4305
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
SmaI CCC'GGG
Cuts at: 1575 1575
Size: 4553
SmlI C'TyrA_G
Cuts at: 1708 2623 3115 3377 1708
Size: 915 492 262 2884
Fragments arranged by size:
2884 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4553
SphI G_CATG'C
Cuts at: 1606 2106 1606
Size: 500 4053
StyI C'CwwG_G
Cuts at: 1271 1297 1687 2041 1271
Size: 26 390 354 3783
Fragments arranged by size:
3783 390 354 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 500 593 1138 1489 2136 2269 2728 2937 500
Size: 93 545 351 647 133 459 209 2116
Fragments arranged by size:
2116 647 545 459 351 209 133 93
TatI w'GTAC_w
Cuts at: 305 1127 4068 305
Size: 822 2941 790
Fragments arranged by size:
2941 822 790
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1176 1208 4070 4102 1176
Size: 32 2862 32 1627
Fragments arranged by size:
2862 1627 32 32
Tth111I GACn'n_nGTC
Cuts at: 2350 2350
Size: 4553
XbaI T'CTAG_A
Cuts at: 1584 1584
Size: 4553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 782 2048 782
Size: 1266 3287
XmaI C'CCGG_G
Cuts at: 1573 1573
Size: 4553
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PasI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BglI BmgBI BmtI BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI