Restriction map for
pF1KB9512
(Circular) (Six-base) MAPSORT of: pF1KB9512.seq Check: 6819 from: 1 to: 4622
pF1KB9512 4622 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 201 619 928 1102 201
Size: 418 309 174 3721
Fragments arranged by size:
3721 418 309 174
AccI GT'mk_AC
Cuts at: 1660 1660
Size: 4622
Acc65I G'GTAC_C
Cuts at: 1027 1638 1027
Size: 611 4011
AclI AA'CG_TT
Cuts at: 4462 4462
Size: 4622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1165 2447 2879 3626 3894 1165
Size: 1282 432 747 268 1893
Fragments arranged by size:
1893 1282 747 432 268
AflIII A'CryG_T
Cuts at: 980 1974 3078 4055 980
Size: 994 1104 977 1547
Fragments arranged by size:
1547 1104 994 977
AgeI A'CCGG_T
Cuts at: 3010 3010
Size: 4622
AlwNI CAG_nnn'CTG
Cuts at: 523 1927 3494 523
Size: 1404 1567 1651
Fragments arranged by size:
1651 1567 1404
ApaI G_GGCC'C
Cuts at: 302 571 302
Size: 269 4353
ApaLI G'TGCA_C
Cuts at: 1242 3392 1242
Size: 2150 2472
ApoI r'AATT_y
Cuts at: 1626 3975 4231 1626
Size: 2349 256 2017
Fragments arranged by size:
2349 2017 256
AseI AT'TA_AT
Cuts at: 20 340 20
Size: 320 4302
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4622
AvaI C'yCGr_G
Cuts at: 139 332 371 1342 1608 1642 139
Size: 193 39 971 266 34 3119
Fragments arranged by size:
3119 971 266 193 39 34
BamHI G'GATC_C
Cuts at: 1647 1647
Size: 4622
BanI G'GyrC_C
Cuts at: 457 1027 1638 2052 2300 2335 457
Size: 570 611 414 248 35 2744
Fragments arranged by size:
2744 611 570 414 248 35
BanII G_rGCy'C
Cuts at: 116 302 493 571 677 1177 1315 1343 1445
Size: 186 191 78 106 500 138 28 102
Cuts at: 1445 1636 2666 116
Size: 191 1030 2072
Fragments arranged by size:
2072 1030 500 191 191 186 138 106
102 78 28
BbeI G_GCGC'C
Cuts at: 2056 2304 2056
Size: 248 4374
BbvCI CC'TCA_GC
Cuts at: 590 590
Size: 4622
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1333 1367 1333
Size: 34 4588
BciVI GTATCCnnnnn_n'
Cuts at: 2520 3281 4622 2520
Size: 761 1341 2520
Fragments arranged by size:
2520 1341 761
BclI T'GATC_A
Cuts at: 2142 2142
Size: 4622
BglI GCCn_nnn'nGGC
Cuts at: 124 124
Size: 4622
BglII A'GATC_T
Cuts at: 2137 4174 2137
Size: 2037 2585
BlpI GC'TnA_GC
Cuts at: 1734 1734
Size: 4622
Bme1580I G_kGCm'C
Cuts at: 302 571 1246 2247 2340 3396 302
Size: 269 675 1001 93 1056 1528
Fragments arranged by size:
1528 1056 1001 675 269 93
BmgBI CAC'GTC
Cuts at: 801 801
Size: 4622
BmrI ACTGGGnnnn_n'
Cuts at: 1834 1915 2250 4394 1834
Size: 81 335 2144 2062
Fragments arranged by size:
2144 2062 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1191 2989 1191
Size: 1798 2824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 533 269
Size: 264 4358
Bpu10I CC'TnA_GC
Cuts at: 590 1571 590
Size: 981 3641
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 761 1798 2677 3169 3467 761
Size: 1037 879 492 298 1916
Fragments arranged by size:
1916 1037 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 841 1155 4326 841
Size: 314 3171 1137
Fragments arranged by size:
3171 1137 314
BsaAI yAC'GTr
Cuts at: 1975 1975
Size: 4622
BsaBI GATnn'nnATC
Cuts at: 6 2136 4173 6
Size: 2130 2037 455
Fragments arranged by size:
2130 2037 455
BsaHI Gr'CG_yC
Cuts at: 2053 2301 3003 4499 2053
Size: 248 702 1496 2176
Fragments arranged by size:
2176 1496 702 248
BsaWI w'CCGG_w
Cuts at: 479 1541 1811 2332 3010 3284 3431 3842 479
Size: 1062 270 521 678 274 147 411 1259
Fragments arranged by size:
1259 1062 678 521 411 274 270 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 493 523 701 731 493
Size: 30 178 30 4384
Fragments arranged by size:
4384 178 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 214 217 332 214
Size: 3 115 4504
Fragments arranged by size:
4504 115 3
BseYI C'CCAG_C
Cuts at: 444 2047 2805 3382 3878 444
Size: 1603 758 577 496 1188
Fragments arranged by size:
1603 1188 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 255 250
Size: 5 4617
BsiEI CG_ry'CG
Cuts at: 104 135 2164 2210 3418 104
Size: 31 2029 46 1208 1308
Fragments arranged by size:
2029 1308 1208 46 31
BsiHKAI G_wGCw'C
Cuts at: 677 1246 1636 2414 2604 3396 677
Size: 569 390 778 190 792 1903
Fragments arranged by size:
1903 792 778 569 390 190
BsmI GAATG_Cn'
Cuts at: 1356 1469 1356
Size: 113 4509
Bsp1286I G_dGCh'C
Cuts at: 116 302 493 571 677 1177 1246 1315 1343
Size: 186 191 78 106 500 69 69 28
Cuts at: 1343 1445 1636 2247 2340 2414 2604 2666 3396
Size: 102 191 611 93 74 190 62 730
Cuts at: 3396 116
Size: 1342
Fragments arranged by size:
1342 730 611 500 191 191 190 186
106 102 93 78 74 69 69 62
28
BspEI T'CCGG_A
Cuts at: 479 3842 479
Size: 3363 1259
BspHI T'CATG_A
Cuts at: 3798 3798
Size: 4622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 201 619 928 1102 1412 1672 1886 2188 2569
Size: 418 309 174 310 260 214 302 381
Cuts at: 2569 201
Size: 2254
Fragments arranged by size:
2254 418 381 310 309 302 260 214
174
BsrBI CCG'CTC
Cuts at: 50 2914 2968 4452 4617 50
Size: 2864 54 1484 165 55
Fragments arranged by size:
2864 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2534 2534
Size: 4622
BsrFI r'CCGG_y
Cuts at: 118 454 1557 2620 2801 3010 118
Size: 336 1103 1063 181 209 1730
Fragments arranged by size:
1730 1103 1063 336 209 181
BssSI C'ACGA_G
Cuts at: 2893 3251 2893
Size: 358 4264
BstAPI GCAn_nnn'nTGC
Cuts at: 1999 1999
Size: 4622
BstBI TT'CG_AA
Cuts at: 2983 2983
Size: 4622
BstYI r'GATC_y
Cuts at: 1 1201 1647 2114 2137 2472 2718 3730 3816
Size: 1200 446 467 23 335 246 1012 86
Cuts at: 3816 3828 4174 1
Size: 12 346 449
Fragments arranged by size:
1200 1012 467 449 446 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 557 621 107
Size: 450 64 4108
Fragments arranged by size:
4108 450 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2572 2753 2916 2572
Size: 181 163 4278
Fragments arranged by size:
4278 181 163
BtsI GCAGTG_nn'
Cuts at: 267 1865 267
Size: 1598 3024
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 851 886 851
Size: 35 4587
DraI TTT'AAA
Cuts at: 1621 1621
Size: 4622
DraIII CAC_nnn'GTG
Cuts at: 600 600
Size: 4622
DrdI GACnn_nn'nnGTC
Cuts at: 1147 2328 3186 1147
Size: 1181 858 2583
Fragments arranged by size:
2583 1181 858
EaeI y'GGCC_r
Cuts at: 132 257 2207 2381 2772 2799 4144 132
Size: 125 1950 174 391 27 1345 610
Fragments arranged by size:
1950 1345 610 391 174 125 27
EagI C'GGCC_G
Cuts at: 132 2207 132
Size: 2075 2547
EarI CTCTTCn'nnn_
Cuts at: 827 2645 2855 827
Size: 1818 210 2594
Fragments arranged by size:
2594 1818 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2830 3140 3286 4432 2830
Size: 310 146 1146 3020
Fragments arranged by size:
3020 1146 310 146
EcoICRI GAG'CTC
Cuts at: 675 1634 675
Size: 959 3663
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 533 1165 2447 2879 3626 3894 269
Size: 264 632 1282 432 747 268 997
Fragments arranged by size:
1282 997 747 632 432 268 264
EcoNI CCTnn'n_nnAGG
Cuts at: 1408 1408
Size: 4622
EcoO109I rG'GnC_Cy
Cuts at: 298 568 816 1605 1761 298
Size: 270 248 789 156 3159
Fragments arranged by size:
3159 789 270 248 156
EcoRI G'AATT_C
Cuts at: 1626 1626
Size: 4622
EcoRV GAT'ATC
Cuts at: 1383 1383
Size: 4622
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2641 2641
Size: 4622
FspI TGC'GCA
Cuts at: 2403 2403
Size: 4622
HaeII r_GCGC'y
Cuts at: 2056 2304 3326 4305 2056
Size: 248 1022 979 2373
Fragments arranged by size:
2373 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 493 525 703 735 757 789 2464 2494 2496
Size: 32 178 32 22 32 1675 30 2
Cuts at: 2496 2526 493
Size: 30 2589
Fragments arranged by size:
2589 1675 178 32 32 32 30 30
22 2
HincII GTy'rAC
Cuts at: 1661 1661
Size: 4622
KasI G'GCGC_C
Cuts at: 2052 2300 2052
Size: 248 4374
KpnI G_GTAC'C
Cuts at: 1031 1642 1031
Size: 611 4011
MluI A'CGCG_T
Cuts at: 4055 4055
Size: 4622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 947 1475 3293 3477 947
Size: 528 1818 184 2092
Fragments arranged by size:
2092 1818 528 184
MscI TGG'CCA
Cuts at: 2383 2383
Size: 4622
MslI CAynn'nnrTG
Cuts at: 1076 2611 2738 1076
Size: 1535 127 2960
Fragments arranged by size:
2960 1535 127
MspA1I CmG'CkG
Cuts at: 147 247 957 1123 1189 1733 2047 2407 3420
Size: 100 710 166 66 544 314 360 1013
Cuts at: 3420 3665 147
Size: 245 1104
Fragments arranged by size:
1104 1013 710 544 360 314 245 166
100 66
NaeI GCC'GGC
Cuts at: 120 456 1559 2803 120
Size: 336 1103 1244 1939
Fragments arranged by size:
1939 1244 1103 336
NarI GG'CG_CC
Cuts at: 2053 2301 2053
Size: 248 4374
NcoI C'CATG_G
Cuts at: 107 107
Size: 4622
NdeI CA'TA_TG
Cuts at: 1389 1389
Size: 4622
NgoMIV G'CCGG_C
Cuts at: 118 454 1557 2801 118
Size: 336 1103 1244 1939
Fragments arranged by size:
1939 1244 1103 336
NspI r_CATG'y
Cuts at: 1675 2175 3082 1675
Size: 500 907 3215
Fragments arranged by size:
3215 907 500
PasI CC'CwG_GG
Cuts at: 1308 1308
Size: 4622
PciI A'CATG_T
Cuts at: 3078 3078
Size: 4622
PflMI CCAn_nnn'nTGG
Cuts at: 308 308
Size: 4622
PfoI T'CCnGG_A
Cuts at: 985 985
Size: 4622
PmeI GTTT'AAAC
Cuts at: 1621 1621
Size: 4622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 3761
Size: 32 4590
PpuMI rG'GwC_Cy
Cuts at: 816 1605 816
Size: 789 3833
PspOMI G'GGCC_C
Cuts at: 298 567 298
Size: 269 4353
PstI C_TGCA'G
Cuts at: 212 1669 2354 212
Size: 1457 685 2480
Fragments arranged by size:
2480 1457 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4622
PvuII CAG'CTG
Cuts at: 247 957 2047 2407 247
Size: 710 1090 360 2462
Fragments arranged by size:
2462 1090 710 360
RsrII CG'GwC_CG
Cuts at: 2817 2817
Size: 4622
SacI G_AGCT'C
Cuts at: 677 1636 677
Size: 959 3663
SalI G'TCGA_C
Cuts at: 1659 1659
Size: 4622
SanDI GG'GwC_CC
Cuts at: 816 816
Size: 4622
SapI GCTCTTCn'nnn_
Cuts at: 2645 2855 2645
Size: 210 4412
SbfI CC_TGCA'GG
Cuts at: 1669 1669
Size: 4622
ScaI AGT'ACT
Cuts at: 4139 4139
Size: 4622
SexAI A'CCwGG_T
Cuts at: 905 961 905
Size: 56 4566
SfcI C'TryA_G
Cuts at: 33 208 723 952 1665 2350 3343 3534 33
Size: 175 515 229 713 685 993 191 1121
Fragments arranged by size:
1121 993 713 685 515 229 191 175
SfoI GGC'GCC
Cuts at: 2054 2302 2054
Size: 248 4374
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4622
SmaI CCC'GGG
Cuts at: 334 373 1644 334
Size: 39 1271 3312
Fragments arranged by size:
3312 1271 39
SmlI C'TyrA_G
Cuts at: 776 1777 2692 3184 3446 776
Size: 1001 915 492 262 1952
Fragments arranged by size:
1952 1001 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4622
SphI G_CATG'C
Cuts at: 1675 2175 1675
Size: 500 4122
StyI C'CwwG_G
Cuts at: 107 743 869 1409 1756 2110 107
Size: 636 126 540 347 354 2619
Fragments arranged by size:
2619 636 540 354 347 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 648 879 1222 2205 2338 2797 3006 648
Size: 231 343 983 133 459 209 2264
Fragments arranged by size:
2264 983 459 343 231 209 133
TatI w'GTAC_w
Cuts at: 4137 4137
Size: 4622
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 701 733 4139 4171 701
Size: 32 3406 32 1152
Fragments arranged by size:
3406 1152 32 32
Tth111I GACn'n_nGTC
Cuts at: 1528 2419 1528
Size: 891 3731
XbaI T'CTAG_A
Cuts at: 1653 1653
Size: 4622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2117 2117
Size: 4622
XmaI C'CCGG_G
Cuts at: 332 371 1642 332
Size: 39 1271 3312
Fragments arranged by size:
3312 1271 39
XmnI GAAnn'nnTTC
Cuts at: 655 655
Size: 4622
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BfrBI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI