Restriction map for
pF1KB9511
(Circular) (Six-base) MAPSORT of: pF1KB9511.seq Check: 1412 from: 1 to: 4271
pF1KB9511 4271 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 314 1309 314
Size: 995 3276
Acc65I G'GTAC_C
Cuts at: 618 1287 618
Size: 669 3602
AclI AA'CG_TT
Cuts at: 4111 4111
Size: 4271
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 497 1208 2096 2528 3275 3543 497
Size: 711 888 432 747 268 1225
Fragments arranged by size:
1225 888 747 711 432 268
AflIII A'CryG_T
Cuts at: 1623 2727 3704 1623
Size: 1104 977 2190
Fragments arranged by size:
2190 1104 977
AgeI A'CCGG_T
Cuts at: 2659 2659
Size: 4271
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1238 1238
Size: 4271
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 994 1026 994
Size: 32 4239
AlwNI CAG_nnn'CTG
Cuts at: 1576 3143 1576
Size: 1567 2704
ApaI G_GGCC'C
Cuts at: 211 211
Size: 4271
ApaLI G'TGCA_C
Cuts at: 3041 3041
Size: 4271
ApoI r'AATT_y
Cuts at: 693 1275 3624 3880 693
Size: 582 2349 256 1084
Fragments arranged by size:
2349 1084 582 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4271
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
AvaI C'yCGr_G
Cuts at: 1291 1291
Size: 4271
BamHI G'GATC_C
Cuts at: 1296 1296
Size: 4271
BanI G'GyrC_C
Cuts at: 618 1287 1701 1949 1984 618
Size: 669 414 248 35 2905
Fragments arranged by size:
2905 669 414 248 35
BanII G_rGCy'C
Cuts at: 211 1285 2315 211
Size: 1074 1030 2167
Fragments arranged by size:
2167 1074 1030
BbeI G_GCGC'C
Cuts at: 1705 1953 1705
Size: 248 4023
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 117 151 117
Size: 34 4237
BciVI GTATCCnnnnn_n'
Cuts at: 294 2169 2930 4271 294
Size: 1875 761 1341 294
Fragments arranged by size:
1875 1341 761 294
BclI T'GATC_A
Cuts at: 1791 1791
Size: 4271
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4271
BglII A'GATC_T
Cuts at: 480 1092 1786 3823 480
Size: 612 694 2037 928
Fragments arranged by size:
2037 928 694 612
BlpI GC'TnA_GC
Cuts at: 1383 1383
Size: 4271
Bme1580I G_kGCm'C
Cuts at: 211 1896 1989 3045 211
Size: 1685 93 1056 1437
Fragments arranged by size:
1685 1437 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1008 1137 1483 1564 1899 4043 1008
Size: 129 346 81 335 2144 1236
Fragments arranged by size:
2144 1236 346 335 129 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2638 2638
Size: 4271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 799 987 751
Size: 48 188 4035
Fragments arranged by size:
4035 188 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1447 2326 2818 3116 1447
Size: 879 492 298 2602
Fragments arranged by size:
2602 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 934 3975 934
Size: 3041 1230
BsaAI yAC'GTr
Cuts at: 1624 1624
Size: 4271
BsaBI GATnn'nnATC
Cuts at: 6 1091 1785 3822 6
Size: 1085 694 2037 455
Fragments arranged by size:
2037 1085 694 455
BsaHI Gr'CG_yC
Cuts at: 1702 1950 2652 4148 1702
Size: 248 702 1496 1825
Fragments arranged by size:
1825 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1460 1981 2659 2933 3080 3491 1460
Size: 521 678 274 147 411 2240
Fragments arranged by size:
2240 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 374 539 374
Size: 165 4106
BseYI C'CCAG_C
Cuts at: 1696 2454 3031 3527 1696
Size: 758 577 496 2440
Fragments arranged by size:
2440 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 141 1813 1859 3067 104
Size: 37 1672 46 1208 1308
Fragments arranged by size:
1672 1308 1208 46 37
BsiHKAI G_wGCw'C
Cuts at: 1285 2063 2253 3045 1285
Size: 778 190 792 2511
Fragments arranged by size:
2511 792 778 190
BsmI GAATG_Cn'
Cuts at: 741 741
Size: 4271
Bsp1286I G_dGCh'C
Cuts at: 211 1285 1896 1989 2063 2253 2315 3045 211
Size: 1074 611 93 74 190 62 730 1437
Fragments arranged by size:
1437 1074 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3491 3491
Size: 4271
BspHI T'CATG_A
Cuts at: 3447 3447
Size: 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1122 1321 1535 1837 2218 1122
Size: 199 214 302 381 3175
Fragments arranged by size:
3175 381 302 214 199
BsrBI CCG'CTC
Cuts at: 50 188 2563 2617 4101 4266 50
Size: 138 2375 54 1484 165 55
Fragments arranged by size:
2375 1484 165 138 55 54
BsrDI GCAATG_nn'
Cuts at: 522 2183 522
Size: 1661 2610
BsrFI r'CCGG_y
Cuts at: 2269 2450 2659 2269
Size: 181 209 3881
Fragments arranged by size:
3881 209 181
BssSI C'ACGA_G
Cuts at: 2542 2900 2542
Size: 358 3913
BstAPI GCAn_nnn'nTGC
Cuts at: 1648 1648
Size: 4271
BstBI TT'CG_AA
Cuts at: 771 2632 771
Size: 1861 2410
BstXI CCAn_nnnn'nTGG
Cuts at: 965 965
Size: 4271
BstYI r'GATC_y
Cuts at: 1 438 480 1092 1296 1763 1786 2121 2367
Size: 437 42 612 204 467 23 335 246
Cuts at: 2367 3379 3465 3477 3823 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 612 467 449 437 346 335 246
204 86 42 23 12
BtgI C'CryG_G
Cuts at: 263 263
Size: 4271
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2221 2402 2565 2221
Size: 181 163 3927
Fragments arranged by size:
3927 181 163
BtsI GCAGTG_nn'
Cuts at: 1514 1514
Size: 4271
DraI TTT'AAA
Cuts at: 1270 1270
Size: 4271
DrdI GACnn_nn'nnGTC
Cuts at: 1977 2835 1977
Size: 858 3413
EaeI y'GGCC_r
Cuts at: 138 762 804 1104 1149 1856 2030 2421 2448
Size: 624 42 300 45 707 174 391 27
Cuts at: 2448 3793 138
Size: 1345 616
Fragments arranged by size:
1345 707 624 616 391 300 174 45
42 27
EagI C'GGCC_G
Cuts at: 138 1856 138
Size: 1718 2553
EarI CTCTTCn'nnn_
Cuts at: 751 2294 2504 751
Size: 1543 210 2518
Fragments arranged by size:
2518 1543 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2479 2789 2935 4081 2479
Size: 310 146 1146 2669
Fragments arranged by size:
2669 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1283 1283
Size: 4271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 497 751 799 987 1208 2096 2528 3275 3543
Size: 254 48 188 221 888 432 747 268
Cuts at: 3543 497
Size: 1225
Fragments arranged by size:
1225 888 747 432 268 254 221 188
48
EcoO109I rG'GnC_Cy
Cuts at: 208 540 1134 1410 208
Size: 332 594 276 3069
Fragments arranged by size:
3069 594 332 276
EcoRI G'AATT_C
Cuts at: 1275 1275
Size: 4271
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 243 2290 243
Size: 2047 2224
FspI TGC'GCA
Cuts at: 2052 2052
Size: 4271
HaeII r_GCGC'y
Cuts at: 1705 1953 2975 3954 1705
Size: 248 1022 979 2022
Fragments arranged by size:
2022 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 759 791 2113 2143 2145 2175 759
Size: 32 1322 30 2 30 2855
Fragments arranged by size:
2855 1322 32 30 30 2
HincII GTy'rAC
Cuts at: 1310 1310
Size: 4271
KasI G'GCGC_C
Cuts at: 1701 1949 1701
Size: 248 4023
KpnI G_GTAC'C
Cuts at: 622 1291 622
Size: 669 3602
MluI A'CGCG_T
Cuts at: 3704 3704
Size: 4271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 635 2942 3126 635
Size: 2307 184 1780
Fragments arranged by size:
2307 1780 184
MscI TGG'CCA
Cuts at: 764 806 1106 1151 2032 764
Size: 42 300 45 881 3003
Fragments arranged by size:
3003 881 300 45 42
MslI CAynn'nnrTG
Cuts at: 1166 2260 2387 1166
Size: 1094 127 3050
Fragments arranged by size:
3050 1094 127
MspA1I CmG'CkG
Cuts at: 173 1382 1696 2056 3069 3314 173
Size: 1209 314 360 1013 245 1130
Fragments arranged by size:
1209 1130 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2452 2452
Size: 4271
NarI GG'CG_CC
Cuts at: 1702 1950 1702
Size: 248 4023
NcoI C'CATG_G
Cuts at: 263 263
Size: 4271
NgoMIV G'CCGG_C
Cuts at: 2450 2450
Size: 4271
NspI r_CATG'y
Cuts at: 132 690 1324 1824 2731 132
Size: 558 634 500 907 1672
Fragments arranged by size:
1672 907 634 558 500
PciI A'CATG_T
Cuts at: 2727 2727
Size: 4271
PmeI GTTT'AAAC
Cuts at: 1270 1270
Size: 4271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 3410
Size: 32 4239
PpuMI rG'GwC_Cy
Cuts at: 540 1134 540
Size: 594 3677
PspOMI G'GGCC_C
Cuts at: 207 207
Size: 4271
PstI C_TGCA'G
Cuts at: 492 1318 2003 492
Size: 826 685 2760
Fragments arranged by size:
2760 826 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4271
PvuII CAG'CTG
Cuts at: 1696 2056 1696
Size: 360 3911
RsrII CG'GwC_CG
Cuts at: 2466 2466
Size: 4271
SacI G_AGCT'C
Cuts at: 1285 1285
Size: 4271
SalI G'TCGA_C
Cuts at: 1308 1308
Size: 4271
SapI GCTCTTCn'nnn_
Cuts at: 2294 2504 2294
Size: 210 4061
SbfI CC_TGCA'GG
Cuts at: 1318 1318
Size: 4271
ScaI AGT'ACT
Cuts at: 3788 3788
Size: 4271
SexAI A'CCwGG_T
Cuts at: 614 614
Size: 4271
SfcI C'TryA_G
Cuts at: 33 488 854 1314 1999 2992 3183 33
Size: 455 366 460 685 993 191 1121
Fragments arranged by size:
1121 993 685 460 455 366 191
SfoI GGC'GCC
Cuts at: 1703 1951 1703
Size: 248 4023
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4271
SmaI CCC'GGG
Cuts at: 1293 1293
Size: 4271
SmlI C'TyrA_G
Cuts at: 1426 2341 2833 3095 1426
Size: 915 492 262 2602
Fragments arranged by size:
2602 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4271
SphI G_CATG'C
Cuts at: 132 690 1324 1824 132
Size: 558 634 500 2579
Fragments arranged by size:
2579 634 558 500
StyI C'CwwG_G
Cuts at: 241 263 1405 1759 241
Size: 22 1142 354 2753
Fragments arranged by size:
2753 1142 354 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 568 1854 1987 2446 2655 568
Size: 1286 133 459 209 2184
Fragments arranged by size:
2184 1286 459 209 133
TatI w'GTAC_w
Cuts at: 3786 3786
Size: 4271
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3788 3820 3788
Size: 32 4239
Tth111I GACn'n_nGTC
Cuts at: 2068 2068
Size: 4271
XbaI T'CTAG_A
Cuts at: 1302 1302
Size: 4271
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 201 1766 201
Size: 1565 2706
XmaI C'CCGG_G
Cuts at: 1291 1291
Size: 4271
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI
BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI