Restriction map for
pF1KB9509
(Circular) (Six-base) MAPSORT of: pF1KB9509.seq Check: 4919 from: 1 to: 4193
pF1KB9509 4193 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1231 1231
Size: 4193
Acc65I G'GTAC_C
Cuts at: 1209 1209
Size: 4193
AclI AA'CG_TT
Cuts at: 4033 4033
Size: 4193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 271 418 760 1078 2018 2450 3197 3465
Size: 71 147 342 318 940 432 747 268
Cuts at: 3465 200
Size: 928
Fragments arranged by size:
940 928 747 432 342 318 268 147
71
AflII C'TTAA_G
Cuts at: 651 651
Size: 4193
AflIII A'CryG_T
Cuts at: 1545 2649 3626 1545
Size: 1104 977 2112
Fragments arranged by size:
2112 1104 977
AgeI A'CCGG_T
Cuts at: 2581 2581
Size: 4193
AleI CACnn'nnGTG
Cuts at: 215 215
Size: 4193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4193
AlwNI CAG_nnn'CTG
Cuts at: 249 783 858 1498 3065 249
Size: 534 75 640 1567 1377
Fragments arranged by size:
1567 1377 640 534 75
ApaLI G'TGCA_C
Cuts at: 2963 2963
Size: 4193
ApoI r'AATT_y
Cuts at: 429 1197 3546 3802 429
Size: 768 2349 256 820
Fragments arranged by size:
2349 820 768 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4193
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
AvaI C'yCGr_G
Cuts at: 1213 1213
Size: 4193
BamHI G'GATC_C
Cuts at: 1218 1218
Size: 4193
BanI G'GyrC_C
Cuts at: 1209 1623 1871 1906 1209
Size: 414 248 35 3496
Fragments arranged by size:
3496 414 248 35
BanII G_rGCy'C
Cuts at: 1207 2237 1207
Size: 1030 3163
BbeI G_GCGC'C
Cuts at: 1627 1875 1627
Size: 248 3945
BbsI GAAGACnn'nnnn_
Cuts at: 415 463 415
Size: 48 4145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 204 238 204
Size: 34 4159
BciVI GTATCCnnnnn_n'
Cuts at: 2091 2852 4193 2091
Size: 761 1341 2091
Fragments arranged by size:
2091 1341 761
BclI T'GATC_A
Cuts at: 1713 1713
Size: 4193
BfrBI ATG'CAT
Cuts at: 443 443
Size: 4193
BglI GCCn_nnn'nGGC
Cuts at: 112 893 112
Size: 781 3412
BglII A'GATC_T
Cuts at: 1708 3745 1708
Size: 2037 2156
BlpI GC'TnA_GC
Cuts at: 1305 1305
Size: 4193
Bme1580I G_kGCm'C
Cuts at: 1818 1911 2967 1818
Size: 93 1056 3044
Fragments arranged by size:
3044 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1113 1405 1486 1821 3965 1113
Size: 292 81 335 2144 1341
Fragments arranged by size:
2144 1341 335 292 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 4193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 541 559 757 937 1059 1096 1111 475
Size: 66 18 198 180 122 37 15 3557
Fragments arranged by size:
3557 198 180 122 66 37 18 15
Bpu10I CC'TnA_GC
Cuts at: 883 883
Size: 4193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1051 1369 2248 2740 3038 1051
Size: 318 879 492 298 2206
Fragments arranged by size:
2206 879 492 318 298
BsaI GGTCTCn'nnnn_
Cuts at: 3897 3897
Size: 4193
BsaAI yAC'GTr
Cuts at: 1546 1546
Size: 4193
BsaBI GATnn'nnATC
Cuts at: 6 356 1707 3744 6
Size: 350 1351 2037 455
Fragments arranged by size:
2037 1351 455 350
BsaHI Gr'CG_yC
Cuts at: 1624 1872 2574 4070 1624
Size: 248 702 1496 1747
Fragments arranged by size:
1747 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1382 1903 2581 2855 3002 3413 1382
Size: 521 678 274 147 411 2162
Fragments arranged by size:
2162 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 730 760 1069 1099 730
Size: 30 309 30 3824
Fragments arranged by size:
3824 309 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 277 289 472 277
Size: 12 183 3998
Fragments arranged by size:
3998 183 12
BseYI C'CCAG_C
Cuts at: 684 1618 2376 2953 3449 684
Size: 934 758 577 496 1428
Fragments arranged by size:
1428 934 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1735 1781 2989 104
Size: 1631 46 1208 1308
Fragments arranged by size:
1631 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1207 1985 2175 2967 1207
Size: 778 190 792 2433
Fragments arranged by size:
2433 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1207 1818 1911 1985 2175 2237 2967 1207
Size: 611 93 74 190 62 730 2433
Fragments arranged by size:
2433 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3413 3413
Size: 4193
BspHI T'CATG_A
Cuts at: 3369 3369
Size: 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 875 1243 1457 1759 2140 875
Size: 368 214 302 381 2928
Fragments arranged by size:
2928 381 368 302 214
BsrBI CCG'CTC
Cuts at: 50 267 1095 2485 2539 4023 4188 50
Size: 217 828 1390 54 1484 165 55
Fragments arranged by size:
1484 1390 828 217 165 55 54
BsrDI GCAATG_nn'
Cuts at: 947 2105 947
Size: 1158 3035
BsrFI r'CCGG_y
Cuts at: 827 1124 2191 2372 2581 827
Size: 297 1067 181 209 2439
Fragments arranged by size:
2439 1067 297 209 181
BssSI C'ACGA_G
Cuts at: 2464 2822 2464
Size: 358 3835
BstAPI GCAn_nnn'nTGC
Cuts at: 1570 1570
Size: 4193
BstBI TT'CG_AA
Cuts at: 2554 2554
Size: 4193
BstXI CCAn_nnnn'nTGG
Cuts at: 327 1074 327
Size: 747 3446
BstYI r'GATC_y
Cuts at: 1 483 688 1218 1685 1708 2043 2289 3301
Size: 482 205 530 467 23 335 246 1012
Cuts at: 3301 3387 3399 3745 1
Size: 86 12 346 449
Fragments arranged by size:
1012 530 482 467 449 346 335 246
205 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 206 2143 2324 2487 206
Size: 1937 181 163 1912
Fragments arranged by size:
1937 1912 181 163
BtsI GCAGTG_nn'
Cuts at: 222 1436 222
Size: 1214 2979
ClaI AT'CG_AT
Cuts at: 1166 1166
Size: 4193
DraI TTT'AAA
Cuts at: 1192 1192
Size: 4193
DrdI GACnn_nn'nnGTC
Cuts at: 169 1899 2757 169
Size: 1730 858 1605
Fragments arranged by size:
1730 1605 858
EaeI y'GGCC_r
Cuts at: 227 1778 1952 2343 2370 3715 227
Size: 1551 174 391 27 1345 705
Fragments arranged by size:
1551 1345 705 391 174 27
EagI C'GGCC_G
Cuts at: 1778 1778
Size: 4193
EarI CTCTTCn'nnn_
Cuts at: 619 2216 2426 619
Size: 1597 210 2386
Fragments arranged by size:
2386 1597 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 130 412 1143 2401 2711 2857 4003 130
Size: 282 731 1258 310 146 1146 320
Fragments arranged by size:
1258 1146 731 320 310 282 146
EcoICRI GAG'CTC
Cuts at: 1205 1205
Size: 4193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 200 271 418 475 541 559 757 760 937
Size: 71 147 57 66 18 198 3 177
Cuts at: 937 1059 1078 1096 1111 2018 2450 3197 3465
Size: 122 19 18 15 907 432 747 268
Cuts at: 3465 200
Size: 928
Fragments arranged by size:
928 907 747 432 268 198 177 147
122 71 66 57 19 18 18 15
3
EcoO109I rG'GnC_Cy
Cuts at: 279 912 1113 1332 279
Size: 633 201 219 3140
Fragments arranged by size:
3140 633 219 201
EcoRI G'AATT_C
Cuts at: 1197 1197
Size: 4193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 4193
FspI TGC'GCA
Cuts at: 1974 1974
Size: 4193
HaeII r_GCGC'y
Cuts at: 529 1627 1875 2897 3876 529
Size: 1098 248 1022 979 846
Fragments arranged by size:
1098 1022 979 846 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 501 533 673 705 831 863 937 969 2035
Size: 32 140 32 126 32 74 32 1066
Cuts at: 2035 2065 2067 2097 501
Size: 30 2 30 2597
Fragments arranged by size:
2597 1066 140 126 74 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 162 588 596 624 1232 162
Size: 426 8 28 608 3123
Fragments arranged by size:
3123 608 426 28 8
KasI G'GCGC_C
Cuts at: 1623 1871 1623
Size: 248 3945
KpnI G_GTAC'C
Cuts at: 1213 1213
Size: 4193
MluI A'CGCG_T
Cuts at: 3626 3626
Size: 4193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 923 2864 3048 923
Size: 1941 184 2068
Fragments arranged by size:
2068 1941 184
MscI TGG'CCA
Cuts at: 1954 1954
Size: 4193
MslI CAynn'nnrTG
Cuts at: 215 2182 2309 215
Size: 1967 127 2099
Fragments arranged by size:
2099 1967 127
MspA1I CmG'CkG
Cuts at: 1304 1618 1978 2991 3236 1304
Size: 314 360 1013 245 2261
Fragments arranged by size:
2261 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2374 2374
Size: 4193
NarI GG'CG_CC
Cuts at: 1624 1872 1624
Size: 248 3945
NcoI C'CATG_G
Cuts at: 107 107
Size: 4193
NgoMIV G'CCGG_C
Cuts at: 2372 2372
Size: 4193
NsiI A_TGCA'T
Cuts at: 445 445
Size: 4193
NspI r_CATG'y
Cuts at: 1246 1746 2653 1246
Size: 500 907 2786
Fragments arranged by size:
2786 907 500
PacI TTA_AT'TAA
Cuts at: 437 437
Size: 4193
PciI A'CATG_T
Cuts at: 2649 2649
Size: 4193
PmeI GTTT'AAAC
Cuts at: 1192 1192
Size: 4193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3332 3364 3332
Size: 32 4161
PpuMI rG'GwC_Cy
Cuts at: 279 1113 279
Size: 834 3359
PstI C_TGCA'G
Cuts at: 854 1240 1925 854
Size: 386 685 3122
Fragments arranged by size:
3122 685 386
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4193
PvuII CAG'CTG
Cuts at: 1618 1978 1618
Size: 360 3833
RsrII CG'GwC_CG
Cuts at: 2388 2388
Size: 4193
SacI G_AGCT'C
Cuts at: 1207 1207
Size: 4193
SalI G'TCGA_C
Cuts at: 1230 1230
Size: 4193
SapI GCTCTTCn'nnn_
Cuts at: 2216 2426 2216
Size: 210 3983
SbfI CC_TGCA'GG
Cuts at: 1240 1240
Size: 4193
ScaI AGT'ACT
Cuts at: 3710 3710
Size: 4193
SexAI A'CCwGG_T
Cuts at: 857 857
Size: 4193
SfcI C'TryA_G
Cuts at: 33 463 670 850 1236 1921 2914 3105 33
Size: 430 207 180 386 685 993 191 1121
Fragments arranged by size:
1121 993 685 430 386 207 191 180
SfoI GGC'GCC
Cuts at: 1625 1873 1625
Size: 248 3945
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4193
SmaI CCC'GGG
Cuts at: 1215 1215
Size: 4193
SmlI C'TyrA_G
Cuts at: 651 1030 1348 2263 2755 3017 651
Size: 379 318 915 492 262 1827
Fragments arranged by size:
1827 915 492 379 318 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4193
SphI G_CATG'C
Cuts at: 1246 1746 1246
Size: 500 3693
StyI C'CwwG_G
Cuts at: 107 1327 1681 107
Size: 1220 354 2619
Fragments arranged by size:
2619 1220 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1776 1909 2368 2577 1776
Size: 133 459 209 3392
Fragments arranged by size:
3392 459 209 133
TatI w'GTAC_w
Cuts at: 3708 3708
Size: 4193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3710 3742 3710
Size: 32 4161
Tth111I GACn'n_nGTC
Cuts at: 1990 1990
Size: 4193
XbaI T'CTAG_A
Cuts at: 546 1224 546
Size: 678 3515
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1688 1688
Size: 4193
XmaI C'CCGG_G
Cuts at: 1213 1213
Size: 4193
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PacI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI ApaI AscI AvrII
BaeI BbvCI BmgBI BmtI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI