Restriction map for
pF1KB9503
(Circular) (Six-base) MAPSORT of: pF1KB9503.seq Check: 2839 from: 1 to: 6917
pF1KB9503 6917 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AatII G_ACGT'C
Cuts at: 3503 3503
Size: 6917
AccI GT'mk_AC
Cuts at: 3284 3734 3955 3284
Size: 450 221 6246
Fragments arranged by size:
6246 450 221
Acc65I G'GTAC_C
Cuts at: 3933 3933
Size: 6917
AclI AA'CG_TT
Cuts at: 6757 6757
Size: 6917
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1193 2609 4742 5174 5527 6189 1193
Size: 1416 2133 432 353 662 1921
Fragments arranged by size:
2133 1921 1416 662 432 353
AflIII A'CryG_T
Cuts at: 2385 3126 4269 6069 6350 2385
Size: 741 1143 1800 281 2952
Fragments arranged by size:
2952 1800 1143 741 281
AleI CACnn'nnGTG
Cuts at: 155 340 155
Size: 185 6732
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 432 1075 432
Size: 643 6274
AlwNI CAG_nnn'CTG
Cuts at: 568 1266 2256 2424 3442 4222 5660 568
Size: 698 990 168 1018 780 1438 1825
Fragments arranged by size:
1825 1438 1018 990 780 698 168
ApaI G_GGCC'C
Cuts at: 1046 1345 2221 2779 3904 1046
Size: 299 876 558 1125 4059
Fragments arranged by size:
4059 1125 876 558 299
ApaLI G'TGCA_C
Cuts at: 5755 5755
Size: 6917
ApoI r'AATT_y
Cuts at: 195 1703 1714 2326 2354 2755 2830 3183 3574
Size: 1508 11 612 28 401 75 353 391
Cuts at: 3574 3853 3921 6270 6526 195
Size: 279 68 2349 256 586
Fragments arranged by size:
2349 1508 612 586 401 391 353 279
256 75 68 28 11
AseI AT'TA_AT
Cuts at: 20 20
Size: 6917
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6917
AvaI C'yCGr_G
Cuts at: 2807 3937 2807
Size: 1130 5787
BamHI G'GATC_C
Cuts at: 1694 3089 3942 1694
Size: 1395 853 4669
Fragments arranged by size:
4669 1395 853
BanI G'GyrC_C
Cuts at: 706 1451 1471 2116 2659 3042 3135 3538 3768
Size: 745 20 645 543 383 93 403 230
Cuts at: 3768 3933 4347 4595 4630 706
Size: 165 414 248 35 2993
Fragments arranged by size:
2993 745 645 543 414 403 383 248
230 165 93 35 20
BanII G_rGCy'C
Cuts at: 1046 1345 2221 2779 3612 3644 3873 3904 3931
Size: 299 876 558 833 32 229 31 27
Cuts at: 3931 4961 1046
Size: 1030 3002
Fragments arranged by size:
3002 1030 876 833 558 299 229 32
31 27
BbeI G_GCGC'C
Cuts at: 2663 4351 4599 2663
Size: 1688 248 4981
Fragments arranged by size:
4981 1688 248
BbsI GAAGACnn'nnnn_
Cuts at: 906 994 1328 2519 906
Size: 88 334 1191 5304
Fragments arranged by size:
5304 1191 334 88
BbvCI CC'TCA_GC
Cuts at: 561 2405 561
Size: 1844 5073
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1994 2028 2788 2822 1994
Size: 34 760 34 6089
Fragments arranged by size:
6089 760 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4815 5871 6917 4815
Size: 1056 1046 4815
Fragments arranged by size:
4815 1056 1046
BclI T'GATC_A
Cuts at: 2426 4437 2426
Size: 2011 4906
BglII A'GATC_T
Cuts at: 1833 4432 6469 1833
Size: 2599 2037 2281
Fragments arranged by size:
2599 2281 2037
BlpI GC'TnA_GC
Cuts at: 4029 4029
Size: 6917
Bme1580I G_kGCm'C
Cuts at: 279 1046 1345 2221 2779 3047 3543 3904 4542
Size: 767 299 876 558 268 496 361 638
Cuts at: 4542 4635 5759 279
Size: 93 1124 1437
Fragments arranged by size:
1437 1124 876 767 638 558 496 361
299 268 93
BmgBI CAC'GTC
Cuts at: 3357 3708 3357
Size: 351 6566
BmrI ACTGGGnnnn_n'
Cuts at: 4129 4210 4545 6689 4129
Size: 81 335 2144 4357
Fragments arranged by size:
4357 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 233 5284 233
Size: 5051 1866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 379 941 1103 1357 1621 2399 2497 3028
Size: 68 562 162 254 264 778 98 531
Cuts at: 3028 3232 3706 311
Size: 204 474 3522
Fragments arranged by size:
3522 778 562 531 474 264 254 204
162 98 68
Bpu10I CC'TnA_GC
Cuts at: 561 941 2405 2723 3140 3872 561
Size: 380 1464 318 417 732 3606
Fragments arranged by size:
3606 1464 732 417 380 318
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 442 1093 2638 3499 4093 4972 5686 5984 442
Size: 651 1545 861 594 879 714 298 1375
Fragments arranged by size:
1545 1375 879 861 714 651 594 298
BsaI GGTCTCn'nnnn_
Cuts at: 1975 2085 6621 1975
Size: 110 4536 2271
Fragments arranged by size:
4536 2271 110
BsaAI yAC'GTr
Cuts at: 2386 4270 2386
Size: 1884 5033
BsaBI GATnn'nnATC
Cuts at: 6 697 1429 1552 2431 4431 6468 6
Size: 691 732 123 879 2000 2037 455
Fragments arranged by size:
2037 2000 879 732 691 455 123
BsaHI Gr'CG_yC
Cuts at: 2450 2660 3500 4348 4596 5298 6794 2450
Size: 210 840 848 248 702 1496 2573
Fragments arranged by size:
2573 1496 848 840 702 248 210
BsaWI w'CCGG_w
Cuts at: 899 1406 3341 4106 4627 5305 5716 5863 6137
Size: 507 1935 765 521 678 411 147 274
Cuts at: 6137 899
Size: 1679
Fragments arranged by size:
1935 1679 765 678 521 507 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1076 1106 2372 2402 1076
Size: 30 1266 30 5591
Fragments arranged by size:
5591 1266 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 376 988 1197 2164 2284 2860 3703 220
Size: 156 612 209 967 120 576 843 3434
Fragments arranged by size:
3434 967 843 612 576 209 156 120
BseYI C'CCAG_C
Cuts at: 682 820 889 1746 2899 3747 3859 4342 5100
Size: 138 69 857 1153 848 112 483 758
Cuts at: 5100 5765 6173 682
Size: 665 408 1426
Fragments arranged by size:
1426 1153 857 848 758 665 483 408
138 112 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1597 2083 1597
Size: 486 6431
BsiEI CG_ry'CG
Cuts at: 104 4459 4505 5735 104
Size: 4355 46 1230 1286
Fragments arranged by size:
4355 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 447 2385 3931 4709 4899 5759 447
Size: 1938 1546 778 190 860 1605
Fragments arranged by size:
1938 1605 1546 860 778 190
BsmI GAATG_Cn'
Cuts at: 802 2693 802
Size: 1891 5026
BsmBI CGTCTCn'nnnn_
Cuts at: 1209 1209
Size: 6917
Bsp1286I G_dGCh'C
Cuts at: 279 447 1046 1345 2221 2385 2779 3047 3543
Size: 168 599 299 876 164 394 268 496
Cuts at: 3543 3612 3644 3873 3904 3931 4542 4635 4709
Size: 69 32 229 31 27 611 93 74
Cuts at: 4709 4899 4961 5759 279
Size: 190 62 798 1437
Fragments arranged by size:
1437 876 798 611 599 496 394 299
268 229 190 168 164 93 74 69
62 32 31 27
BspHI T'CATG_A
Cuts at: 515 5349 515
Size: 4834 2083
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3720 3967 4181 4483 4864 3720
Size: 247 214 302 381 5773
Fragments arranged by size:
5773 381 302 247 214
BsrBI CCG'CTC
Cuts at: 50 1469 2138 3605 5209 5263 6747 6912 50
Size: 1419 669 1467 1604 54 1484 165 55
Fragments arranged by size:
1604 1484 1467 1419 669 165 55 54
BsrDI GCAATG_nn'
Cuts at: 998 1057 4829 998
Size: 59 3772 3086
Fragments arranged by size:
3772 3086 59
BsrFI r'CCGG_y
Cuts at: 4915 5096 4915
Size: 181 6736
BssSI C'ACGA_G
Cuts at: 5188 5896 5188
Size: 708 6209
BstAPI GCAn_nnn'nTGC
Cuts at: 571 2256 4294 571
Size: 1685 2038 3194
Fragments arranged by size:
3194 2038 1685
BstBI TT'CG_AA
Cuts at: 5278 5278
Size: 6917
BstXI CCAn_nnnn'nTGG
Cuts at: 1005 2526 2887 1005
Size: 1521 361 5035
Fragments arranged by size:
5035 1521 361
BstYI r'GATC_y
Cuts at: 1 308 662 1067 1097 1638 1694 1833 3089
Size: 307 354 405 30 541 56 139 1256
Cuts at: 3089 3942 4409 4432 4767 5013 5319 5331 5417
Size: 853 467 23 335 246 306 12 86
Cuts at: 5417 6469 1
Size: 1052 449
Fragments arranged by size:
1256 1052 853 541 467 449 405 354
335 307 306 246 139 86 56 30
23 12
BstZ17I GTA'TAC
Cuts at: 3735 3735
Size: 6917
BtgI C'CryG_G
Cuts at: 2180 3543 2180
Size: 1363 5554
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 577 801 1648 3144 4867 5048 5211 577
Size: 224 847 1496 1723 181 163 2283
Fragments arranged by size:
2283 1723 1496 847 224 181 163
BtsI GCAGTG_nn'
Cuts at: 1010 3825 4160 1010
Size: 2815 335 3767
Fragments arranged by size:
3767 2815 335
ClaI AT'CG_AT
Cuts at: 2763 2763
Size: 6917
DraI TTT'AAA
Cuts at: 3916 3916
Size: 6917
DrdI GACnn_nn'nnGTC
Cuts at: 4623 5967 4623
Size: 1344 5573
EaeI y'GGCC_r
Cuts at: 464 1268 2057 2970 4502 4676 5067 5094 6439
Size: 804 789 913 1532 174 391 27 1345
Cuts at: 6439 464
Size: 942
Fragments arranged by size:
1532 1345 942 913 804 789 391 174
27
EagI C'GGCC_G
Cuts at: 4502 4502
Size: 6917
EarI CTCTTCn'nnn_
Cuts at: 197 1459 1531 2585 3752 4940 5150 197
Size: 1262 72 1054 1167 1188 210 1964
Fragments arranged by size:
1964 1262 1188 1167 1054 210 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2281 5125 5867 6013 6727 2281
Size: 2844 742 146 714 2471
Fragments arranged by size:
2844 2471 742 714 146
EcoICRI GAG'CTC
Cuts at: 3929 3929
Size: 6917
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 379 941 1103 1193 1357 1621 2399 2497
Size: 68 562 162 90 164 264 778 98
Cuts at: 2497 2609 3028 3232 3706 4742 5174 5527 6189
Size: 112 419 204 474 1036 432 353 662
Cuts at: 6189 311
Size: 1039
Fragments arranged by size:
1039 1036 778 662 562 474 432 419
353 264 204 164 162 112 98 90
68
EcoNI CCTnn'n_nnAGG
Cuts at: 3776 3776
Size: 6917
EcoO109I rG'GnC_Cy
Cuts at: 1341 1342 1563 1780 2217 2218 2421 2712 2776
Size: 1 221 217 437 1 203 291 64
Cuts at: 2776 3900 3901 4056 1341
Size: 1124 1 155 4202
Fragments arranged by size:
4202 1124 437 291 221 217 203 155
64 1 1 1
EcoRI G'AATT_C
Cuts at: 3574 3921 3574
Size: 347 6570
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 252 4936 252
Size: 4684 2233
FspI TGC'GCA
Cuts at: 1516 4698 1516
Size: 3182 3735
FspAI rTGC'GCAy
Cuts at: 1516 1516
Size: 6917
HaeII r_GCGC'y
Cuts at: 2663 3727 4351 4599 5829 6600 2663
Size: 1064 624 248 1230 771 2980
Fragments arranged by size:
2980 1230 1064 771 624 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1102 1134 1594 1626 2372 2404 2555 2587 2920
Size: 32 460 32 746 32 151 32 333
Cuts at: 2920 2952 3133 3165 4759 4789 4791 4821 1102
Size: 32 181 32 1594 30 2 30 3198
Fragments arranged by size:
3198 1594 746 460 333 181 151 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1169 2298 3956 1169
Size: 1129 1658 4130
Fragments arranged by size:
4130 1658 1129
KasI G'GCGC_C
Cuts at: 2659 4347 4595 2659
Size: 1688 248 4981
Fragments arranged by size:
4981 1688 248
KpnI G_GTAC'C
Cuts at: 3937 3937
Size: 6917
MluI A'CGCG_T
Cuts at: 6350 6350
Size: 6917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 244 336 3326 5676 5860 244
Size: 92 2990 2350 184 1301
Fragments arranged by size:
2990 2350 1301 184 92
MscI TGG'CCA
Cuts at: 466 1270 2059 4678 466
Size: 804 789 2619 2705
Fragments arranged by size:
2705 2619 804 789
MslI CAynn'nnrTG
Cuts at: 155 340 490 1003 1031 2524 2736 4906 5033
Size: 185 150 513 28 1493 212 2170 127
Cuts at: 5033 155
Size: 2039
Fragments arranged by size:
2170 2039 1493 513 212 185 150 127
28
MspA1I CmG'CkG
Cuts at: 289 3507 3545 4028 4342 4702 5486 5731 289
Size: 3218 38 483 314 360 784 245 1475
Fragments arranged by size:
3218 1475 784 483 360 314 245 38
NaeI GCC'GGC
Cuts at: 5098 5098
Size: 6917
NarI GG'CG_CC
Cuts at: 2660 4348 4596 2660
Size: 1688 248 4981
Fragments arranged by size:
4981 1688 248
NgoMIV G'CCGG_C
Cuts at: 5096 5096
Size: 6917
NspI r_CATG'y
Cuts at: 1818 3130 3970 4470 6073 1818
Size: 1312 840 500 1603 2662
Fragments arranged by size:
2662 1603 1312 840 500
PciI A'CATG_T
Cuts at: 3126 6069 3126
Size: 2943 3974
PflMI CCAn_nnn'nTGG
Cuts at: 2496 2626 2496
Size: 130 6787
PfoI T'CCnGG_A
Cuts at: 2879 2879
Size: 6917
PmeI GTTT'AAAC
Cuts at: 3916 3916
Size: 6917
PmlI CAC'GTG
Cuts at: 2386 2386
Size: 6917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 429 461 5363 5395 429
Size: 32 4902 32 1951
Fragments arranged by size:
4902 1951 32 32
PpuMI rG'GwC_Cy
Cuts at: 1563 1780 2421 2712 1563
Size: 217 641 291 5768
Fragments arranged by size:
5768 641 291 217
PshAI GACnn'nnGTC
Cuts at: 1174 1174
Size: 6917
PspOMI G'GGCC_C
Cuts at: 1042 1341 2217 2775 3900 1042
Size: 299 876 558 1125 4059
Fragments arranged by size:
4059 1125 876 558 299
PstI C_TGCA'G
Cuts at: 551 3149 3731 3964 4649 551
Size: 2598 582 233 685 2819
Fragments arranged by size:
2819 2598 685 582 233
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6917
PvuII CAG'CTG
Cuts at: 4342 4702 4342
Size: 360 6557
RsrII CG'GwC_CG
Cuts at: 5112 5112
Size: 6917
SacI G_AGCT'C
Cuts at: 3931 3931
Size: 6917
SacII CC_GC'GG
Cuts at: 3546 3546
Size: 6917
SalI G'TCGA_C
Cuts at: 3954 3954
Size: 6917
SanDI GG'GwC_CC
Cuts at: 1563 1563
Size: 6917
SapI GCTCTTCn'nnn_
Cuts at: 1459 1531 4940 5150 1459
Size: 72 3409 210 3226
Fragments arranged by size:
3409 3226 210 72
SbfI CC_TGCA'GG
Cuts at: 3964 3964
Size: 6917
ScaI AGT'ACT
Cuts at: 6434 6434
Size: 6917
SfcI C'TryA_G
Cuts at: 33 547 1346 3145 3229 3281 3727 3960 4645
Size: 514 799 1799 84 52 446 233 685
Cuts at: 4645 5613 5804 33
Size: 968 191 1146
Fragments arranged by size:
1799 1146 968 799 685 514 446 233
191 84 52
SfoI GGC'GCC
Cuts at: 2661 4349 4597 2661
Size: 1688 248 4981
Fragments arranged by size:
4981 1688 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6917
SmaI CCC'GGG
Cuts at: 3939 3939
Size: 6917
SmlI C'TyrA_G
Cuts at: 421 1072 2653 3478 4072 4987 5701 5963 421
Size: 651 1581 825 594 915 714 262 1375
Fragments arranged by size:
1581 1375 915 825 714 651 594 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6917
SphI G_CATG'C
Cuts at: 3970 4470 3970
Size: 500 6417
StuI AGG'CCT
Cuts at: 479 559 2482 479
Size: 80 1923 4914
Fragments arranged by size:
4914 1923 80
StyI C'CwwG_G
Cuts at: 474 1046 1184 1634 2120 2663 2941 2996 3197
Size: 572 138 450 486 543 278 55 201
Cuts at: 3197 3764 3895 4051 4405 474
Size: 567 131 156 354 2986
Fragments arranged by size:
2986 572 567 543 486 450 354 278
201 156 138 131 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 903 3460 4500 4633 5092 5301 903
Size: 2557 1040 133 459 209 2519
Fragments arranged by size:
2557 2519 1040 459 209 133
TatI w'GTAC_w
Cuts at: 1499 2863 6432 1499
Size: 1364 3569 1984
Fragments arranged by size:
3569 1984 1364
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1455 1487 6434 6466 1455
Size: 32 4947 32 1906
Fragments arranged by size:
4947 1906 32 32
Tth111I GACn'n_nGTC
Cuts at: 1765 2452 2644 4714 1765
Size: 687 192 2070 3968
Fragments arranged by size:
3968 2070 687 192
XbaI T'CTAG_A
Cuts at: 3948 3948
Size: 6917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 286 2670 3003 3783 4412 286
Size: 2384 333 780 629 2791
Fragments arranged by size:
2791 2384 780 629 333
XmaI C'CCGG_G
Cuts at: 3937 3937
Size: 6917
ZraI GAC'GTC
Cuts at: 3501 3501
Size: 6917
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AloI AscI AvrII
BaeI BfrBI BglI BmtI BsiWI BspEI BsrGI BssHII
BstEII Bsu36I CspCI DraIII EcoRV FseI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI