Restriction map for
pF1KB9502
(Circular) (Six-base) MAPSORT of: pF1KB9502.seq Check: 8244 from: 1 to: 6632
pF1KB9502 6632 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 489 2377 2984 3670 489
Size: 1888 607 686 3451
Fragments arranged by size:
3451 1888 686 607
Acc65I G'GTAC_C
Cuts at: 1396 3615 3648 1396
Size: 2219 33 4380
Fragments arranged by size:
4380 2219 33
AclI AA'CG_TT
Cuts at: 6472 6472
Size: 6632
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1061 1267 1459 1700 1808 1823 2610 2716 4457
Size: 206 192 241 108 15 787 106 1741
Cuts at: 4457 4889 5242 5904 1061
Size: 432 353 662 1789
Fragments arranged by size:
1789 1741 787 662 432 353 241 206
192 108 106 15
AflIII A'CryG_T
Cuts at: 299 2082 3984 5784 6065 299
Size: 1783 1902 1800 281 866
Fragments arranged by size:
1902 1800 1783 866 281
AleI CACnn'nnGTG
Cuts at: 3040 3040
Size: 6632
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 219 251 219
Size: 32 6600
AlwNI CAG_nnn'CTG
Cuts at: 724 3625 3937 5375 724
Size: 2901 312 1438 1981
Fragments arranged by size:
2901 1981 1438 312
ApaLI G'TGCA_C
Cuts at: 1880 5470 1880
Size: 3590 3042
ApoI r'AATT_y
Cuts at: 811 900 1099 1225 1338 1724 1860 2275 2287
Size: 89 199 126 113 386 136 415 12
Cuts at: 2287 2363 3636 5985 6241 811
Size: 76 1273 2349 256 1202
Fragments arranged by size:
2349 1273 1202 415 386 256 199 136
126 113 89 76 12
AseI AT'TA_AT
Cuts at: 20 20
Size: 6632
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6632
AvaI C'yCGr_G
Cuts at: 159 3652 159
Size: 3493 3139
BamHI G'GATC_C
Cuts at: 2651 3657 2651
Size: 1006 5626
BanI G'GyrC_C
Cuts at: 417 779 1329 1396 3042 3615 3648 4062 4310
Size: 362 550 67 1646 573 33 414 248
Cuts at: 4310 4345 417
Size: 35 2704
Fragments arranged by size:
2704 1646 573 550 414 362 248 67
35 33
BanII G_rGCy'C
Cuts at: 436 3646 4676 436
Size: 3210 1030 2392
Fragments arranged by size:
3210 2392 1030
BbeI G_GCGC'C
Cuts at: 4066 4314 4066
Size: 248 6384
BbsI GAAGACnn'nnnn_
Cuts at: 1255 2144 2186 2237 2756 3328 1255
Size: 889 42 51 519 572 4559
Fragments arranged by size:
4559 889 572 519 51 42
BciVI GTATCCnnnnn_n'
Cuts at: 1813 3539 4530 5586 6632 1813
Size: 1726 991 1056 1046 1813
Fragments arranged by size:
1813 1726 1056 1046 991
BclI T'GATC_A
Cuts at: 2316 4152 2316
Size: 1836 4796
BglI GCCn_nnn'nGGC
Cuts at: 132 132
Size: 6632
BglII A'GATC_T
Cuts at: 1137 2056 2707 4147 6184 1137
Size: 919 651 1440 2037 1585
Fragments arranged by size:
2037 1585 1440 919 651
BlpI GC'TnA_GC
Cuts at: 3744 3744
Size: 6632
Bme1580I G_kGCm'C
Cuts at: 1884 4257 4350 5474 1884
Size: 2373 93 1124 3042
Fragments arranged by size:
3042 2373 1124 93
BmgBI CAC'GTC
Cuts at: 502 502
Size: 6632
BmrI ACTGGGnnnn_n'
Cuts at: 368 811 3844 3925 4260 6404 368
Size: 443 3033 81 335 2144 596
Fragments arranged by size:
3033 2144 596 443 335 81
BmtI G_CTAG'C
Cuts at: 1469 1496 1469
Size: 27 6605
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1641 4999 1641
Size: 3358 3274
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 729 2743 3060 729
Size: 2014 317 4301
Fragments arranged by size:
4301 2014 317
Bpu10I CC'TnA_GC
Cuts at: 754 754
Size: 6632
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1023 1210 3808 4687 5401 5699 1023
Size: 187 2598 879 714 298 1956
Fragments arranged by size:
2598 1956 879 714 298 187
BsaI GGTCTCn'nnnn_
Cuts at: 183 6336 183
Size: 6153 479
BsaAI yAC'GTr
Cuts at: 3133 3985 3133
Size: 852 5780
BsaBI GATnn'nnATC
Cuts at: 6 671 2321 4146 6183 6
Size: 665 1650 1825 2037 455
Fragments arranged by size:
2037 1825 1650 665 455
BsaHI Gr'CG_yC
Cuts at: 4063 4311 5013 6509 4063
Size: 248 702 1496 4186
Fragments arranged by size:
4186 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3821 4342 5020 5431 5578 5852 3821
Size: 521 678 411 147 274 4601
Fragments arranged by size:
4601 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 577 607 1755 1785 577
Size: 30 1148 30 5424
Fragments arranged by size:
5424 1148 30 30
BseYI C'CCAG_C
Cuts at: 153 4057 4815 5480 5888 153
Size: 3904 758 665 408 897
Fragments arranged by size:
3904 897 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2599 3274 2599
Size: 675 5957
BsiEI CG_ry'CG
Cuts at: 104 4174 4220 5450 104
Size: 4070 46 1230 1286
Fragments arranged by size:
4070 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 473 1593 1884 2270 3646 4424 4614 5474 473
Size: 1120 291 386 1376 778 190 860 1631
Fragments arranged by size:
1631 1376 1120 860 778 386 291 190
BsmI GAATG_Cn'
Cuts at: 1647 1647
Size: 6632
Bsp1286I G_dGCh'C
Cuts at: 436 473 1593 1884 2270 3646 4257 4350 4424
Size: 37 1120 291 386 1376 611 93 74
Cuts at: 4424 4614 4676 5474 436
Size: 190 62 798 1594
Fragments arranged by size:
1594 1376 1120 798 611 386 291 190
93 74 62 37
BspHI T'CATG_A
Cuts at: 452 645 3603 5064 452
Size: 193 2958 1461 2020
Fragments arranged by size:
2958 2020 1461 193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1384 1861 3216 3682 3896 4198 4579 1384
Size: 477 1355 466 214 302 381 3437
Fragments arranged by size:
3437 1355 477 466 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4924 4978 6462 6627 50
Size: 4874 54 1484 165 55
Fragments arranged by size:
4874 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4544 4544
Size: 6632
BsrFI r'CCGG_y
Cuts at: 4630 4811 4630
Size: 181 6451
BsrGI T'GTAC_A
Cuts at: 2878 2878
Size: 6632
BssSI C'ACGA_G
Cuts at: 4903 5611 4903
Size: 708 5924
BstAPI GCAn_nnn'nTGC
Cuts at: 4009 4009
Size: 6632
BstBI TT'CG_AA
Cuts at: 4993 4993
Size: 6632
BstEII G'GTnAC_C
Cuts at: 2899 2899
Size: 6632
BstYI r'GATC_y
Cuts at: 1 1048 1137 2056 2651 2707 3657 4124 4147
Size: 1047 89 919 595 56 950 467 23
Cuts at: 4147 4482 4728 5034 5046 5132 6184 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 1047 950 919 595 467 449 335
306 246 89 86 56 23 12
BstZ17I GTA'TAC
Cuts at: 490 2378 490
Size: 1888 4744
BtgI C'CryG_G
Cuts at: 2647 2647
Size: 6632
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3226 4582 4763 4926 3226
Size: 1356 181 163 4932
Fragments arranged by size:
4932 1356 181 163
BtsI GCAGTG_nn'
Cuts at: 1884 3875 1884
Size: 1991 4641
ClaI AT'CG_AT
Cuts at: 612 612
Size: 6632
DraI TTT'AAA
Cuts at: 2852 2943 3631 2852
Size: 91 688 5853
Fragments arranged by size:
5853 688 91
DraIII CAC_nnn'GTG
Cuts at: 1258 3447 1258
Size: 2189 4443
DrdI GACnn_nn'nnGTC
Cuts at: 4338 5682 4338
Size: 1344 5288
EaeI y'GGCC_r
Cuts at: 1353 2644 4217 4391 4782 4809 6154 1353
Size: 1291 1573 174 391 27 1345 1831
Fragments arranged by size:
1831 1573 1345 1291 391 174 27
EagI C'GGCC_G
Cuts at: 4217 4217
Size: 6632
EarI CTCTTCn'nnn_
Cuts at: 1059 1320 1598 1952 2258 4655 4865 1059
Size: 261 278 354 306 2397 210 2826
Fragments arranged by size:
2826 2397 354 306 278 261 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4840 5582 5728 6442 4840
Size: 742 146 714 5030
Fragments arranged by size:
5030 742 714 146
EcoICRI GAG'CTC
Cuts at: 3644 3644
Size: 6632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 729 1061 1267 1459 1700 1808 1823 2610 2716
Size: 332 206 192 241 108 15 787 106
Cuts at: 2716 2743 3060 4457 4889 5242 5904 729
Size: 27 317 1397 432 353 662 1457
Fragments arranged by size:
1457 1397 787 662 432 353 332 317
241 206 192 108 106 27 15
EcoNI CCTnn'n_nnAGG
Cuts at: 1841 1841
Size: 6632
EcoO109I rG'GnC_Cy
Cuts at: 1735 3771 1735
Size: 2036 4596
EcoRI G'AATT_C
Cuts at: 900 3636 900
Size: 2736 3896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1070 2500 4651 1070
Size: 1430 2151 3051
Fragments arranged by size:
3051 2151 1430
FspI TGC'GCA
Cuts at: 4413 4413
Size: 6632
HaeII r_GCGC'y
Cuts at: 120 4066 4314 5544 6315 120
Size: 3946 248 1230 771 437
Fragments arranged by size:
3946 1230 771 437 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 338 370 577 609 2692 2724 3140 3172 4474
Size: 32 207 32 2083 32 416 32 1302
Cuts at: 4474 4504 4506 4536 338
Size: 30 2 30 2434
Fragments arranged by size:
2434 2083 1302 416 207 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3671 3671
Size: 6632
HindIII A'AGCT_T
Cuts at: 446 3178 446
Size: 2732 3900
KasI G'GCGC_C
Cuts at: 4062 4310 4062
Size: 248 6384
KpnI G_GTAC'C
Cuts at: 1400 3619 3652 1400
Size: 2219 33 4380
Fragments arranged by size:
4380 2219 33
MfeI C'AATT_G
Cuts at: 2300 2300
Size: 6632
MluI A'CGCG_T
Cuts at: 6065 6065
Size: 6632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 284 5391 5575 284
Size: 5107 184 1341
Fragments arranged by size:
5107 1341 184
MscI TGG'CCA
Cuts at: 1355 2646 4393 1355
Size: 1291 1747 3594
Fragments arranged by size:
3594 1747 1291
MslI CAynn'nnrTG
Cuts at: 2327 3040 3200 4621 4748 2327
Size: 713 160 1421 127 4211
Fragments arranged by size:
4211 1421 713 160 127
MspA1I CmG'CkG
Cuts at: 217 2677 2964 3111 3743 4057 4417 5201 5446
Size: 2460 287 147 632 314 360 784 245
Cuts at: 5446 217
Size: 1403
Fragments arranged by size:
2460 1403 784 632 360 314 287 245
147
NaeI GCC'GGC
Cuts at: 4813 4813
Size: 6632
NarI GG'CG_CC
Cuts at: 4063 4311 4063
Size: 248 6384
NcoI C'CATG_G
Cuts at: 2647 2647
Size: 6632
NgoMIV G'CCGG_C
Cuts at: 4811 4811
Size: 6632
NheI G'CTAG_C
Cuts at: 1465 1492 1465
Size: 27 6605
NspI r_CATG'y
Cuts at: 126 303 2086 3478 3685 4185 5788 126
Size: 177 1783 1392 207 500 1603 970
Fragments arranged by size:
1783 1603 1392 970 500 207 177
PciI A'CATG_T
Cuts at: 299 2082 5784 299
Size: 1783 3702 1147
Fragments arranged by size:
3702 1783 1147
PflMI CCAn_nnn'nTGG
Cuts at: 1552 1552
Size: 6632
PfoI T'CCnGG_A
Cuts at: 2091 2091
Size: 6632
PmeI GTTT'AAAC
Cuts at: 3631 3631
Size: 6632
PmlI CAC'GTG
Cuts at: 3133 3133
Size: 6632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5078 5110 5078
Size: 32 6600
PpuMI rG'GwC_Cy
Cuts at: 1735 1735
Size: 6632
PsiI TTA'TAA
Cuts at: 1231 2813 1231
Size: 1582 5050
PstI C_TGCA'G
Cuts at: 216 841 1323 1818 2019 2165 3227 3679 4364
Size: 625 482 495 201 146 1062 452 685
Cuts at: 4364 216
Size: 2484
Fragments arranged by size:
2484 1062 685 625 495 482 452 201
146
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6632
PvuII CAG'CTG
Cuts at: 2677 2964 3111 4057 4417 2677
Size: 287 147 946 360 4892
Fragments arranged by size:
4892 946 360 287 147
RsrII CG'GwC_CG
Cuts at: 4827 4827
Size: 6632
SacI G_AGCT'C
Cuts at: 3646 3646
Size: 6632
SalI G'TCGA_C
Cuts at: 3669 3669
Size: 6632
SapI GCTCTTCn'nnn_
Cuts at: 1320 1598 2258 4655 4865 1320
Size: 278 660 2397 210 3087
Fragments arranged by size:
3087 2397 660 278 210
SbfI CC_TGCA'GG
Cuts at: 3679 3679
Size: 6632
ScaI AGT'ACT
Cuts at: 6149 6149
Size: 6632
SexAI A'CCwGG_T
Cuts at: 1254 1254
Size: 6632
SfcI C'TryA_G
Cuts at: 33 212 837 1319 1652 1814 2015 2021 2161
Size: 179 625 482 333 162 201 6 140
Cuts at: 2161 2420 3223 3675 4360 5328 5519 33
Size: 259 803 452 685 968 191 1146
Fragments arranged by size:
1146 968 803 685 625 482 452 333
259 201 191 179 162 140 6
SfoI GGC'GCC
Cuts at: 4064 4312 4064
Size: 248 6384
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6632
SmaI CCC'GGG
Cuts at: 161 3654 161
Size: 3493 3139
SmlI C'TyrA_G
Cuts at: 1038 1189 3787 4702 5416 5678 1038
Size: 151 2598 915 714 262 1992
Fragments arranged by size:
2598 1992 915 714 262 151
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6632
SphI G_CATG'C
Cuts at: 126 3685 4185 126
Size: 3559 500 2573
Fragments arranged by size:
3559 2573 500
SspI AAT'ATT
Cuts at: 532 2339 532
Size: 1807 4825
StyI C'CwwG_G
Cuts at: 961 1286 1400 2045 2381 2647 3766 4120 961
Size: 325 114 645 336 266 1119 354 3473
Fragments arranged by size:
3473 1119 645 354 336 325 266 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4215 4348 4807 5016 4215
Size: 133 459 209 5831
Fragments arranged by size:
5831 459 209 133
TatI w'GTAC_w
Cuts at: 2236 2590 2878 6147 2236
Size: 354 288 3269 2721
Fragments arranged by size:
3269 2721 354 288
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2465 2497 2781 2813 3117 3149 6149 6181 2465
Size: 32 284 32 304 32 3000 32 2916
Fragments arranged by size:
3000 2916 304 284 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3535 4429 3535
Size: 894 5738
XbaI T'CTAG_A
Cuts at: 3663 3663
Size: 6632
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 481 2645 4127 481
Size: 2164 1482 2986
Fragments arranged by size:
2986 2164 1482
XmaI C'CCGG_G
Cuts at: 159 3652 159
Size: 3493 3139
XmnI GAAnn'nnTTC
Cuts at: 904 1099 3203 904
Size: 195 2104 4333
Fragments arranged by size:
4333 2104 195
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BseRI BsiWI
BsmBI BspEI BssHII BstXI Bsu36I CspCI EcoRV FseI
FspAI HpaI NdeI NotI NruI NsiI PacI PasI
PshAI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI