Restriction map for
pF1KB9501
(Circular) (Six-base) MAPSORT of: pF1KB9501.seq Check: 5006 from: 1 to: 6473
pF1KB9501 6473 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 3511 3511
Size: 6473
Acc65I G'GTAC_C
Cuts at: 3489 3489
Size: 6473
AclI AA'CG_TT
Cuts at: 6313 6313
Size: 6473
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 343 385 691 1148 1348 1405 1535 2530
Size: 44 42 306 457 200 57 130 995
Cuts at: 2530 4298 4730 5083 5745 299
Size: 1768 432 353 662 1027
Fragments arranged by size:
1768 1027 995 662 457 432 353 306
200 130 57 44 42
AflIII A'CryG_T
Cuts at: 3002 3825 5625 5906 3002
Size: 823 1800 281 3569
Fragments arranged by size:
3569 1800 823 281
AhdI GACnn_n'nnGTC
Cuts at: 3421 3421
Size: 6473
AleI CACnn'nnGTG
Cuts at: 2261 2261
Size: 6473
AlwNI CAG_nnn'CTG
Cuts at: 3778 5216 3778
Size: 1438 5035
ApaLI G'TGCA_C
Cuts at: 505 5311 505
Size: 4806 1667
ApoI r'AATT_y
Cuts at: 797 902 2037 2725 3290 3477 5826 6082 797
Size: 105 1135 688 565 187 2349 256 1188
Fragments arranged by size:
2349 1188 1135 688 565 256 187 105
AseI AT'TA_AT
Cuts at: 20 20
Size: 6473
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6473
AvaI C'yCGr_G
Cuts at: 3493 3493
Size: 6473
AvrII C'CTAG_G
Cuts at: 1983 1983
Size: 6473
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1920 1953 1920
Size: 33 6440
BamHI G'GATC_C
Cuts at: 1481 3498 1481
Size: 2017 4456
BanI G'GyrC_C
Cuts at: 1902 2243 2425 3111 3489 3903 4151 4186 1902
Size: 341 182 686 378 414 248 35 4189
Fragments arranged by size:
4189 686 414 378 341 248 182 35
BanII G_rGCy'C
Cuts at: 1034 2072 2438 3487 4517 1034
Size: 1038 366 1049 1030 2990
Fragments arranged by size:
2990 1049 1038 1030 366
BbeI G_GCGC'C
Cuts at: 2247 3907 4155 2247
Size: 1660 248 4565
Fragments arranged by size:
4565 1660 248
BbsI GAAGACnn'nnnn_
Cuts at: 3098 3417 3098
Size: 319 6154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 2914 2948 90
Size: 34 2790 34 3615
Fragments arranged by size:
3615 2790 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4371 5427 6473 4371
Size: 1056 1046 4371
Fragments arranged by size:
4371 1056 1046
BclI T'GATC_A
Cuts at: 1229 1350 3993 1229
Size: 121 2643 3709
Fragments arranged by size:
3709 2643 121
BglI GCCn_nnn'nGGC
Cuts at: 2157 2157
Size: 6473
BglII A'GATC_T
Cuts at: 282 544 2337 2390 3988 6025 282
Size: 262 1793 53 1598 2037 730
Fragments arranged by size:
2037 1793 1598 730 262 53
BlpI GC'TnA_GC
Cuts at: 3585 3585
Size: 6473
Bme1580I G_kGCm'C
Cuts at: 509 4098 4191 5315 509
Size: 3589 93 1124 1667
Fragments arranged by size:
3589 1667 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 527 680 905 1956 2138 3685 3766 4101 6245
Size: 153 225 1051 182 1547 81 335 2144
Cuts at: 6245 527
Size: 755
Fragments arranged by size:
2144 1547 1051 755 335 225 182 153
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4840 4840
Size: 6473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1865 1880 2004 2139 3397 3451 1865
Size: 15 124 135 1258 54 4887
Fragments arranged by size:
4887 1258 135 124 54 15
Bpu10I CC'TnA_GC
Cuts at: 3197 3197
Size: 6473
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 727 736 1684 3649 4528 5242 5540 206
Size: 521 9 948 1965 879 714 298 1139
Fragments arranged by size:
1965 1139 948 879 714 521 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 610 2728 6177 610
Size: 2118 3449 906
Fragments arranged by size:
3449 2118 906
BsaAI yAC'GTr
Cuts at: 3826 3826
Size: 6473
BsaBI GATnn'nnATC
Cuts at: 6 412 1672 2494 2621 3987 6024 6
Size: 406 1260 822 127 1366 2037 455
Fragments arranged by size:
2037 1366 1260 822 455 406 127
BsaHI Gr'CG_yC
Cuts at: 2244 3904 4152 4854 6350 2244
Size: 1660 248 702 1496 2367
Fragments arranged by size:
2367 1660 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3662 4183 4861 5272 5419 5693 3662
Size: 521 678 411 147 274 4442
Fragments arranged by size:
4442 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 218 248 821 851 2172 2202 2528 2558 2801
Size: 30 573 30 1321 30 326 30 243
Cuts at: 2801 2817 2831 2847 218
Size: 16 14 16 3844
Fragments arranged by size:
3844 1321 573 326 243 30 30 30
30 16 16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 143 784 2697 143
Size: 641 1913 3919
Fragments arranged by size:
3919 1913 641
BseYI C'CCAG_C
Cuts at: 191 3301 3898 4656 5321 5729 191
Size: 3110 597 758 665 408 935
Fragments arranged by size:
3110 935 758 665 597 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2285 2627 3065 2285
Size: 342 438 5693
Fragments arranged by size:
5693 438 342
BsiEI CG_ry'CG
Cuts at: 104 4015 4061 5291 104
Size: 3911 46 1230 1286
Fragments arranged by size:
3911 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 509 1034 3487 4265 4455 5315 509
Size: 525 2453 778 190 860 1667
Fragments arranged by size:
2453 1667 860 778 525 190
BsmI GAATG_Cn'
Cuts at: 186 3373 186
Size: 3187 3286
Bsp1286I G_dGCh'C
Cuts at: 509 1034 2072 2438 3487 4098 4191 4265 4455
Size: 525 1038 366 1049 611 93 74 190
Cuts at: 4455 4517 5315 509
Size: 62 798 1667
Fragments arranged by size:
1667 1049 1038 798 611 525 366 190
93 74 62
BspHI T'CATG_A
Cuts at: 1940 4905 1940
Size: 2965 3508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 737 2260 3523 3737 4039 4420 737
Size: 1523 1263 214 302 381 2790
Fragments arranged by size:
2790 1523 1263 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4765 4819 6303 6468 50
Size: 4715 54 1484 165 55
Fragments arranged by size:
4715 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3448 4385 3448
Size: 937 5536
BsrFI r'CCGG_y
Cuts at: 1006 4471 4652 1006
Size: 3465 181 2827
Fragments arranged by size:
3465 2827 181
BsrGI T'GTAC_A
Cuts at: 395 395
Size: 6473
BssSI C'ACGA_G
Cuts at: 2461 4744 5452 2461
Size: 2283 708 3482
Fragments arranged by size:
3482 2283 708
BstAPI GCAn_nnn'nTGC
Cuts at: 790 1546 2122 2425 3326 3850 790
Size: 756 576 303 901 524 3413
Fragments arranged by size:
3413 901 756 576 524 303
BstBI TT'CG_AA
Cuts at: 4834 4834
Size: 6473
BstEII G'GTnAC_C
Cuts at: 459 1059 459
Size: 600 5873
BstXI CCAn_nnnn'nTGG
Cuts at: 1071 1418 1071
Size: 347 6126
BstYI r'GATC_y
Cuts at: 1 282 544 861 1481 1667 2337 2390 3498
Size: 281 262 317 620 186 670 53 1108
Cuts at: 3498 3965 3988 4323 4569 4875 4887 4973 6025
Size: 467 23 335 246 306 12 86 1052
Cuts at: 6025 1
Size: 449
Fragments arranged by size:
1108 1052 670 620 467 449 335 317
306 281 262 246 186 86 53 23
12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4423 4604 4767 4423
Size: 181 163 6129
Fragments arranged by size:
6129 181 163
BtsI GCAGTG_nn'
Cuts at: 1743 3053 3186 3716 1743
Size: 1310 133 530 4500
Fragments arranged by size:
4500 1310 530 133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 543 578 543
Size: 35 6438
DraI TTT'AAA
Cuts at: 3472 3472
Size: 6473
DrdI GACnn_nn'nnGTC
Cuts at: 2227 4179 5523 2227
Size: 1952 1344 3177
Fragments arranged by size:
3177 1952 1344
EaeI y'GGCC_r
Cuts at: 335 1740 4058 4232 4623 4650 5995 335
Size: 1405 2318 174 391 27 1345 813
Fragments arranged by size:
2318 1405 1345 813 391 174 27
EagI C'GGCC_G
Cuts at: 4058 4058
Size: 6473
EarI CTCTTCn'nnn_
Cuts at: 772 1039 1160 2201 2890 4496 4706 772
Size: 267 121 1041 689 1606 210 2539
Fragments arranged by size:
2539 1606 1041 689 267 210 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4681 5423 5569 6283 4681
Size: 742 146 714 4871
Fragments arranged by size:
4871 742 714 146
EcoICRI GAG'CTC
Cuts at: 1032 3485 1032
Size: 2453 4020
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 343 385 691 1148 1348 1405 1535 1865
Size: 44 42 306 457 200 57 130 330
Cuts at: 1865 1880 2004 2139 2530 3397 3451 4298 4730
Size: 15 124 135 391 867 54 847 432
Cuts at: 4730 5083 5745 299
Size: 353 662 1027
Fragments arranged by size:
1027 867 847 662 457 432 391 353
330 306 200 135 130 124 57 54
44 42 15
EcoNI CCTnn'n_nnAGG
Cuts at: 224 378 3016 224
Size: 154 2638 3681
Fragments arranged by size:
3681 2638 154
EcoO109I rG'GnC_Cy
Cuts at: 3612 3612
Size: 6473
EcoRI G'AATT_C
Cuts at: 3477 3477
Size: 6473
EcoRV GAT'ATC
Cuts at: 252 252
Size: 6473
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 390 723 4492 390
Size: 333 3769 2371
Fragments arranged by size:
3769 2371 333
FspI TGC'GCA
Cuts at: 763 4254 763
Size: 3491 2982
HaeII r_GCGC'y
Cuts at: 2247 3907 4155 5385 6156 2247
Size: 1660 248 1230 771 2564
Fragments arranged by size:
2564 1660 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 372 404 443 475 529 561 1347 1379 1440
Size: 32 39 32 54 32 786 32 61
Cuts at: 1440 1472 1957 1989 2528 2560 2725 2757 2801
Size: 32 485 32 539 32 165 32 44
Cuts at: 2801 2833 3177 3209 3407 3439 4315 4345 4347
Size: 32 344 32 198 32 876 30 2
Cuts at: 4347 4377 372
Size: 30 2468
Fragments arranged by size:
2468 876 786 539 485 344 198 165
61 54 44 39 32 32 32 32
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 822 1314 3138 3512 822
Size: 492 1824 374 3783
Fragments arranged by size:
3783 1824 492 374
HindIII A'AGCT_T
Cuts at: 1654 1654
Size: 6473
HpaI GTT'AAC
Cuts at: 3138 3138
Size: 6473
KasI G'GCGC_C
Cuts at: 2243 3903 4151 2243
Size: 1660 248 4565
Fragments arranged by size:
4565 1660 248
KpnI G_GTAC'C
Cuts at: 3493 3493
Size: 6473
MfeI C'AATT_G
Cuts at: 2744 2744
Size: 6473
MluI A'CGCG_T
Cuts at: 5906 5906
Size: 6473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 851 2514 5232 5416 851
Size: 1663 2718 184 1908
Fragments arranged by size:
2718 1908 1663 184
MscI TGG'CCA
Cuts at: 337 4234 337
Size: 3897 2576
MslI CAynn'nnrTG
Cuts at: 2261 2461 4462 4589 2261
Size: 200 2001 127 4145
Fragments arranged by size:
4145 2001 200 127
MspA1I CmG'CkG
Cuts at: 195 363 485 1071 1679 2422 3584 3898 4258
Size: 168 122 586 608 743 1162 314 360
Cuts at: 4258 5042 5287 195
Size: 784 245 1381
Fragments arranged by size:
1381 1162 784 743 608 586 360 314
245 168 122
NaeI GCC'GGC
Cuts at: 4654 4654
Size: 6473
NarI GG'CG_CC
Cuts at: 2244 3904 4152 2244
Size: 1660 248 4565
Fragments arranged by size:
4565 1660 248
NgoMIV G'CCGG_C
Cuts at: 4652 4652
Size: 6473
NspI r_CATG'y
Cuts at: 741 762 1858 3006 3526 4026 5629 741
Size: 21 1096 1148 520 500 1603 1585
Fragments arranged by size:
1603 1585 1148 1096 520 500 21
PciI A'CATG_T
Cuts at: 3002 5625 3002
Size: 2623 3850
PfoI T'CCnGG_A
Cuts at: 1604 3428 1604
Size: 1824 4649
PmeI GTTT'AAAC
Cuts at: 3472 3472
Size: 6473
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4919 4951 4919
Size: 32 6441
PshAI GACnn'nnGTC
Cuts at: 142 142
Size: 6473
PstI C_TGCA'G
Cuts at: 1263 3181 3520 4205 1263
Size: 1918 339 685 3531
Fragments arranged by size:
3531 1918 685 339
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6473
PvuII CAG'CTG
Cuts at: 195 363 485 1071 2422 3898 4258 195
Size: 168 122 586 1351 1476 360 2410
Fragments arranged by size:
2410 1476 1351 586 360 168 122
RsrII CG'GwC_CG
Cuts at: 4668 4668
Size: 6473
SacI G_AGCT'C
Cuts at: 1034 3487 1034
Size: 2453 4020
SalI G'TCGA_C
Cuts at: 3510 3510
Size: 6473
SapI GCTCTTCn'nnn_
Cuts at: 772 1039 2890 4496 4706 772
Size: 267 1851 1606 210 2539
Fragments arranged by size:
2539 1851 1606 267 210
SbfI CC_TGCA'GG
Cuts at: 3520 3520
Size: 6473
ScaI AGT'ACT
Cuts at: 2443 5990 2443
Size: 3547 2926
SfcI C'TryA_G
Cuts at: 33 1259 3177 3516 4201 5169 5360 33
Size: 1226 1918 339 685 968 191 1146
Fragments arranged by size:
1918 1226 1146 968 685 339 191
SfoI GGC'GCC
Cuts at: 2245 3905 4153 2245
Size: 1660 248 4565
Fragments arranged by size:
4565 1660 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6473
SmaI CCC'GGG
Cuts at: 3495 3495
Size: 6473
SmlI C'TyrA_G
Cuts at: 221 742 751 1663 3628 4543 5257 5519 221
Size: 521 9 912 1965 915 714 262 1175
Fragments arranged by size:
1965 1175 915 912 714 521 262 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6473
SphI G_CATG'C
Cuts at: 741 762 3526 4026 741
Size: 21 2764 500 3188
Fragments arranged by size:
3188 2764 500 21
SspI AAT'ATT
Cuts at: 1267 2977 1267
Size: 1710 4763
StuI AGG'CCT
Cuts at: 2160 2160
Size: 6473
StyI C'CwwG_G
Cuts at: 1983 3348 3607 3961 1983
Size: 1365 259 354 4495
Fragments arranged by size:
4495 1365 354 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3129 4056 4189 4648 4857 3129
Size: 927 133 459 209 4745
Fragments arranged by size:
4745 927 459 209 133
TatI w'GTAC_w
Cuts at: 395 2441 5988 395
Size: 2046 3547 880
Fragments arranged by size:
3547 2046 880
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 218 250 821 853 2817 2849 5990 6022 218
Size: 32 571 32 1964 32 3141 32 669
Fragments arranged by size:
3141 1964 669 571 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2212 4270 2212
Size: 2058 4415
XbaI T'CTAG_A
Cuts at: 3504 3504
Size: 6473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2588 2955 3968 2588
Size: 367 1013 5093
Fragments arranged by size:
5093 1013 367
XmaI C'CCGG_G
Cuts at: 3493 3493
Size: 6473
XmnI GAAnn'nnTTC
Cuts at: 284 284
Size: 6473
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PmeI PpiI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AlfI AloI ApaI
AscI BbvCI BfrBI BmgBI BmtI BsiWI BsmBI BspEI
BssHII BstZ17I Bsu36I BtgI ClaI DraIII FseI FspAI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI