Restriction map for
pF1KB9500
(Circular) (Six-base) MAPSORT of: pF1KB9500.seq Check: 8082 from: 1 to: 5138
pF1KB9500 5138 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 2176 2176
Size: 5138
Acc65I G'GTAC_C
Cuts at: 2112 2154 2112
Size: 42 5096
AclI AA'CG_TT
Cuts at: 4978 4978
Size: 5138
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1982 2963 3395 3748 4410 1982
Size: 981 432 353 662 2710
Fragments arranged by size:
2710 981 662 432 353
AflII C'TTAA_G
Cuts at: 367 367
Size: 5138
AflIII A'CryG_T
Cuts at: 2490 4290 4571 2490
Size: 1800 281 3057
Fragments arranged by size:
3057 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1068 1068
Size: 5138
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 992 1024 992
Size: 32 5106
AlwNI CAG_nnn'CTG
Cuts at: 2443 3881 2443
Size: 1438 3700
ApaLI G'TGCA_C
Cuts at: 266 3976 266
Size: 3710 1428
ApoI r'AATT_y
Cuts at: 383 1031 1756 1767 1866 2040 2142 4491 4747
Size: 648 725 11 99 174 102 2349 256
Cuts at: 4747 383
Size: 774
Fragments arranged by size:
2349 774 725 648 256 174 102 99
11
AseI AT'TA_AT
Cuts at: 20 1019 20
Size: 999 4139
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5138
AvaI C'yCGr_G
Cuts at: 2158 2158
Size: 5138
BamHI G'GATC_C
Cuts at: 2163 2163
Size: 5138
BanI G'GyrC_C
Cuts at: 238 2112 2154 2568 2816 2851 238
Size: 1874 42 414 248 35 2525
Fragments arranged by size:
2525 1874 414 248 42 35
BanII G_rGCy'C
Cuts at: 815 2152 3182 815
Size: 1337 1030 2771
Fragments arranged by size:
2771 1337 1030
BbeI G_GCGC'C
Cuts at: 2572 2820 2572
Size: 248 4890
BbsI GAAGACnn'nnnn_
Cuts at: 488 1828 1861 1993 488
Size: 1340 33 132 3633
Fragments arranged by size:
3633 1340 132 33
BbvCI CC'TCA_GC
Cuts at: 2128 2128
Size: 5138
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 769 803 769
Size: 34 5104
BciVI GTATCCnnnnn_n'
Cuts at: 3036 4092 5138 3036
Size: 1056 1046 3036
Fragments arranged by size:
3036 1056 1046
BclI T'GATC_A
Cuts at: 1250 1805 2658 1250
Size: 555 853 3730
Fragments arranged by size:
3730 853 555
BfrBI ATG'CAT
Cuts at: 592 1080 592
Size: 488 4650
BglII A'GATC_T
Cuts at: 669 1493 2653 4690 669
Size: 824 1160 2037 1117
Fragments arranged by size:
2037 1160 1117 824
BlpI GC'TnA_GC
Cuts at: 791 2250 791
Size: 1459 3679
Bme1580I G_kGCm'C
Cuts at: 241 270 2763 2856 3980 241
Size: 29 2493 93 1124 1399
Fragments arranged by size:
2493 1399 1124 93 29
BmrI ACTGGGnnnn_n'
Cuts at: 1103 2350 2431 2766 4910 1103
Size: 1247 81 335 2144 1331
Fragments arranged by size:
2144 1331 1247 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3505 3505
Size: 5138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 248
Size: 5138
Bpu10I CC'TnA_GC
Cuts at: 2074 2128 2074
Size: 54 5084
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 662 2314 3193 3907 4205 662
Size: 1652 879 714 298 1595
Fragments arranged by size:
1652 1595 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1539 1658 4842 1539
Size: 119 3184 1835
Fragments arranged by size:
3184 1835 119
BsaAI yAC'GTr
Cuts at: 2491 2491
Size: 5138
BsaBI GATnn'nnATC
Cuts at: 6 2652 4689 6
Size: 2646 2037 455
Fragments arranged by size:
2646 2037 455
BsaHI Gr'CG_yC
Cuts at: 2569 2817 3519 5015 2569
Size: 248 702 1496 2692
Fragments arranged by size:
2692 1496 702 248
BsaWI w'CCGG_w
Cuts at: 397 1862 2327 2848 3526 3937 4084 4358 397
Size: 1465 465 521 678 411 147 274 1177
Fragments arranged by size:
1465 1177 678 521 465 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 851 881 992 1022 851
Size: 30 111 30 4967
Fragments arranged by size:
4967 111 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 872 1504 872
Size: 632 4506
BseYI C'CCAG_C
Cuts at: 2563 3321 3986 4394 2563
Size: 758 665 408 3307
Fragments arranged by size:
3307 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 2680 2726 3956 104
Size: 2576 46 1230 1286
Fragments arranged by size:
2576 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 270 2152 2930 3120 3980 270
Size: 1882 778 190 860 1428
Fragments arranged by size:
1882 1428 860 778 190
BsmI GAATG_Cn'
Cuts at: 594 1711 594
Size: 1117 4021
Bsp1286I G_dGCh'C
Cuts at: 241 270 815 2152 2763 2856 2930 3120 3182
Size: 29 545 1337 611 93 74 190 62
Cuts at: 3182 3980 241
Size: 798 1399
Fragments arranged by size:
1399 1337 798 611 545 190 93 74
62 29
BspEI T'CCGG_A
Cuts at: 1862 1862
Size: 5138
BspHI T'CATG_A
Cuts at: 1604 3570 1604
Size: 1966 3172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2188 2402 2704 3085 2188
Size: 214 302 381 4241
Fragments arranged by size:
4241 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3430 3484 4968 5133 50
Size: 3380 54 1484 165 55
Fragments arranged by size:
3380 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1895 3050 1895
Size: 1155 3983
BsrFI r'CCGG_y
Cuts at: 1678 3136 3317 1678
Size: 1458 181 3499
Fragments arranged by size:
3499 1458 181
BssSI C'ACGA_G
Cuts at: 893 3409 4117 893
Size: 2516 708 1914
Fragments arranged by size:
2516 1914 708
BstAPI GCAn_nnn'nTGC
Cuts at: 801 2515 801
Size: 1714 3424
BstBI TT'CG_AA
Cuts at: 3499 3499
Size: 5138
BstXI CCAn_nnnn'nTGG
Cuts at: 127 1274 2123 127
Size: 1147 849 3142
Fragments arranged by size:
3142 1147 849
BstYI r'GATC_y
Cuts at: 1 669 1263 1493 2163 2630 2653 2988 3234
Size: 668 594 230 670 467 23 335 246
Cuts at: 3234 3540 3552 3638 4690 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 670 668 594 467 449 335 306
246 230 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3088 3269 3432 3088
Size: 181 163 4794
Fragments arranged by size:
4794 181 163
BtsI GCAGTG_nn'
Cuts at: 550 2381 550
Size: 1831 3307
DraI TTT'AAA
Cuts at: 2137 2137
Size: 5138
DrdI GACnn_nn'nnGTC
Cuts at: 2844 4188 2844
Size: 1344 3794
EaeI y'GGCC_r
Cuts at: 846 1578 1934 2013 2723 2897 3288 3315 4660
Size: 732 356 79 710 174 391 27 1345
Cuts at: 4660 846
Size: 1324
Fragments arranged by size:
1345 1324 732 710 391 356 174 79
27
EagI C'GGCC_G
Cuts at: 2723 2723
Size: 5138
EarI CTCTTCn'nnn_
Cuts at: 1432 1537 1958 3161 3371 1432
Size: 105 421 1203 210 3199
Fragments arranged by size:
3199 1203 421 210 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3346 4088 4234 4948 3346
Size: 742 146 714 3536
Fragments arranged by size:
3536 742 714 146
EcoICRI GAG'CTC
Cuts at: 2150 2150
Size: 5138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 1982 2963 3395 3748 4410 248
Size: 1734 981 432 353 662 976
Fragments arranged by size:
1734 981 976 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 2277 2277
Size: 5138
EcoRI G'AATT_C
Cuts at: 1866 2040 2142 1866
Size: 174 102 4862
Fragments arranged by size:
4862 174 102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1641 1936 3157 1641
Size: 295 1221 3622
Fragments arranged by size:
3622 1221 295
FspI TGC'GCA
Cuts at: 2919 2919
Size: 5138
HaeII r_GCGC'y
Cuts at: 2572 2820 4050 4821 2572
Size: 248 1230 771 2889
Fragments arranged by size:
2889 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 1054 1086 1095 1127 1498 1530 1538
Size: 32 859 32 9 32 371 32 8
Cuts at: 1538 1570 2980 3010 3012 3042 163
Size: 32 1410 30 2 30 2259
Fragments arranged by size:
2259 1410 859 371 32 32 32 32
32 30 30 9 8 2
HincII GTy'rAC
Cuts at: 219 1114 2177 219
Size: 895 1063 3180
Fragments arranged by size:
3180 1063 895
HindIII A'AGCT_T
Cuts at: 1338 1338
Size: 5138
KasI G'GCGC_C
Cuts at: 2568 2816 2568
Size: 248 4890
KpnI G_GTAC'C
Cuts at: 2116 2158 2116
Size: 42 5096
MfeI C'AATT_G
Cuts at: 2033 2033
Size: 5138
MluI A'CGCG_T
Cuts at: 4571 4571
Size: 5138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 254 534 683 3897 4081 254
Size: 280 149 3214 184 1311
Fragments arranged by size:
3214 1311 280 184 149
MscI TGG'CCA
Cuts at: 848 1580 1936 2015 2899 848
Size: 732 356 79 884 3087
Fragments arranged by size:
3087 884 732 356 79
MslI CAynn'nnrTG
Cuts at: 125 244 3127 3254 125
Size: 119 2883 127 2009
Fragments arranged by size:
2883 2009 127 119
MspA1I CmG'CkG
Cuts at: 1732 2249 2563 2923 3707 3952 1732
Size: 517 314 360 784 245 2918
Fragments arranged by size:
2918 784 517 360 314 245
NaeI GCC'GGC
Cuts at: 3319 3319
Size: 5138
NarI GG'CG_CC
Cuts at: 2569 2817 2569
Size: 248 4890
NdeI CA'TA_TG
Cuts at: 122 122
Size: 5138
NgoMIV G'CCGG_C
Cuts at: 3317 3317
Size: 5138
NsiI A_TGCA'T
Cuts at: 594 1082 594
Size: 488 4650
NspI r_CATG'y
Cuts at: 136 2191 2691 4294 136
Size: 2055 500 1603 980
Fragments arranged by size:
2055 1603 980 500
PacI TTA_AT'TAA
Cuts at: 2084 2084
Size: 5138
PciI A'CATG_T
Cuts at: 4290 4290
Size: 5138
PmeI GTTT'AAAC
Cuts at: 2137 2137
Size: 5138
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 992 1024 3584 3616 992
Size: 32 2560 32 2514
Fragments arranged by size:
2560 2514 32 32
PshAI GACnn'nnGTC
Cuts at: 1096 1096
Size: 5138
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 860 892 860
Size: 32 5106
PstI C_TGCA'G
Cuts at: 975 1638 2185 2870 975
Size: 663 547 685 3243
Fragments arranged by size:
3243 685 663 547
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5138
PvuII CAG'CTG
Cuts at: 1732 2563 2923 1732
Size: 831 360 3947
Fragments arranged by size:
3947 831 360
RsrII CG'GwC_CG
Cuts at: 3333 3333
Size: 5138
SacI G_AGCT'C
Cuts at: 2152 2152
Size: 5138
SalI G'TCGA_C
Cuts at: 2175 2175
Size: 5138
SapI GCTCTTCn'nnn_
Cuts at: 3161 3371 3161
Size: 210 4928
SbfI CC_TGCA'GG
Cuts at: 2185 2185
Size: 5138
ScaI AGT'ACT
Cuts at: 4655 4655
Size: 5138
SfcI C'TryA_G
Cuts at: 33 914 971 1284 1634 2181 2866 3834 4025
Size: 881 57 313 350 547 685 968 191
Cuts at: 4025 33
Size: 1146
Fragments arranged by size:
1146 968 881 685 547 350 313 191
57
SfoI GGC'GCC
Cuts at: 2570 2818 2570
Size: 248 4890
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5138
SmaI CCC'GGG
Cuts at: 2160 2160
Size: 5138
SmlI C'TyrA_G
Cuts at: 367 677 2293 3208 3922 4184 367
Size: 310 1616 915 714 262 1321
Fragments arranged by size:
1616 1321 915 714 310 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5138
SphI G_CATG'C
Cuts at: 2191 2691 2191
Size: 500 4638
SspI AAT'ATT
Cuts at: 470 470
Size: 5138
StuI AGG'CCT
Cuts at: 1282 1282
Size: 5138
StyI C'CwwG_G
Cuts at: 1136 2272 2626 1136
Size: 1136 354 3648
Fragments arranged by size:
3648 1136 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 898 2721 2854 3313 3522 898
Size: 1823 133 459 209 2514
Fragments arranged by size:
2514 1823 459 209 133
TatI w'GTAC_w
Cuts at: 618 4653 618
Size: 4035 1103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4655 4687 4655
Size: 32 5106
Tth111I GACn'n_nGTC
Cuts at: 937 2935 937
Size: 1998 3140
XbaI T'CTAG_A
Cuts at: 1011 2169 1011
Size: 1158 3980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2633 2633
Size: 5138
XmaI C'CCGG_G
Cuts at: 2158 2158
Size: 5138
XmnI GAAnn'nnTTC
Cuts at: 1919 1919
Size: 5138
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NsiI NspI PacI
PciI PmeI PpiI PshAI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AleI AlfI ApaI AscI
AvrII BaeI BglI BmgBI BmtI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI NcoI NheI
NotI NruI PasI PflMI PfoI PmlI PpuMI PsiI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI