Restriction map for
pF1KB9499
(Circular) (Six-base) MAPSORT of: pF1KB9499.seq Check: 9969 from: 1 to: 6515
pF1KB9499 6515 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2220 2220
Size: 6515
AatII G_ACGT'C
Cuts at: 2476 2476
Size: 6515
AccI GT'mk_AC
Cuts at: 1915 3118 3553 1915
Size: 1203 435 4877
Fragments arranged by size:
4877 1203 435
Acc65I G'GTAC_C
Cuts at: 3531 3531
Size: 6515
AclI AA'CG_TT
Cuts at: 6355 6355
Size: 6515
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 653 1450 3264 4340 4772 5125 5787 325
Size: 328 797 1814 1076 432 353 662 1053
Fragments arranged by size:
1814 1076 1053 797 662 432 353 328
AfeI AGC'GCT
Cuts at: 3285 3285
Size: 6515
AflIII A'CryG_T
Cuts at: 3867 5667 5948 3867
Size: 1800 281 4434
Fragments arranged by size:
4434 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1489 1489
Size: 6515
AlwNI CAG_nnn'CTG
Cuts at: 238 631 942 1713 2337 2500 3337 3456 3820
Size: 393 311 771 624 163 837 119 364
Cuts at: 3820 5258 238
Size: 1438 1495
Fragments arranged by size:
1495 1438 837 771 624 393 364 311
163 119
ApaI G_GGCC'C
Cuts at: 2486 2596 2486
Size: 110 6405
ApaLI G'TGCA_C
Cuts at: 5353 5353
Size: 6515
ApoI r'AATT_y
Cuts at: 427 859 1144 2182 3519 5868 6124 427
Size: 432 285 1038 1337 2349 256 818
Fragments arranged by size:
2349 1337 1038 818 432 285 256
AseI AT'TA_AT
Cuts at: 20 1271 20
Size: 1251 5264
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6515
AvaI C'yCGr_G
Cuts at: 597 1304 2016 2865 3343 3535 597
Size: 707 712 849 478 192 3577
Fragments arranged by size:
3577 849 712 707 478 192
BamHI G'GATC_C
Cuts at: 3540 3540
Size: 6515
BanI G'GyrC_C
Cuts at: 958 1847 3432 3531 3945 4193 4228 958
Size: 889 1585 99 414 248 35 3245
Fragments arranged by size:
3245 1585 889 414 248 99 35
BanII G_rGCy'C
Cuts at: 119 2030 2486 2596 3226 3529 4559 119
Size: 1911 456 110 630 303 1030 2075
Fragments arranged by size:
2075 1911 1030 630 456 303 110
BbeI G_GCGC'C
Cuts at: 3949 4197 3949
Size: 248 6267
BbsI GAAGACnn'nnnn_
Cuts at: 2102 2650 3367 2102
Size: 548 717 5250
Fragments arranged by size:
5250 717 548
BbvCI CC'TCA_GC
Cuts at: 1232 1232
Size: 6515
BciVI GTATCCnnnnn_n'
Cuts at: 4413 5469 6515 4413
Size: 1056 1046 4413
Fragments arranged by size:
4413 1056 1046
BclI T'GATC_A
Cuts at: 4035 4035
Size: 6515
BglI GCCn_nnn'nGGC
Cuts at: 1788 1788
Size: 6515
BglII A'GATC_T
Cuts at: 363 4030 6067 363
Size: 3667 2037 811
Fragments arranged by size:
3667 2037 811
BlpI GC'TnA_GC
Cuts at: 1312 2450 3627 1312
Size: 1138 1177 4200
Fragments arranged by size:
4200 1177 1138
Bme1580I G_kGCm'C
Cuts at: 1850 2486 2596 3437 4140 4233 5357 1850
Size: 636 110 841 703 93 1124 3008
Fragments arranged by size:
3008 1124 841 703 636 110 93
BmrI ACTGGGnnnn_n'
Cuts at: 2353 3727 3808 4143 6287 2353
Size: 1374 81 335 2144 2581
Fragments arranged by size:
2581 2144 1374 335 81
BmtI G_CTAG'C
Cuts at: 2297 2297
Size: 6515
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 978 4882 978
Size: 3904 2611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 973 997 1061 1210 1426 1720 1735 2098
Size: 605 24 64 149 216 294 15 363
Cuts at: 2098 2572 2694 2999 3378 368
Size: 474 122 305 379 3505
Fragments arranged by size:
3505 605 474 379 363 305 294 216
149 122 64 24 15
Bpu10I CC'TnA_GC
Cuts at: 935 1214 1232 2755 935
Size: 279 18 1523 4695
Fragments arranged by size:
4695 1523 279 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1369 1435 3691 4570 5284 5582 1369
Size: 66 2256 879 714 298 2302
Fragments arranged by size:
2302 2256 879 714 298 66
BsaI GGTCTCn'nnnn_
Cuts at: 3315 6219 3315
Size: 2904 3611
BsaAI yAC'GTr
Cuts at: 3868 3868
Size: 6515
BsaBI GATnn'nnATC
Cuts at: 6 4029 6066 6
Size: 4023 2037 455
Fragments arranged by size:
4023 2037 455
BsaHI Gr'CG_yC
Cuts at: 2473 3946 4194 4896 6392 2473
Size: 1473 248 702 1496 2596
Fragments arranged by size:
2596 1496 1473 702 248
BsaWI w'CCGG_w
Cuts at: 1574 3704 4225 4903 5314 5461 5735 1574
Size: 2130 521 678 411 147 274 2354
Fragments arranged by size:
2354 2130 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1660 1690 1660
Size: 30 6485
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 202 910 913 1564 2054 2109 3156 3282 202
Size: 708 3 651 490 55 1047 126 3435
Fragments arranged by size:
3435 1047 708 651 490 126 55 3
BseYI C'CCAG_C
Cuts at: 193 2170 2733 3940 4698 5363 5771 193
Size: 1977 563 1207 758 665 408 937
Fragments arranged by size:
1977 1207 937 758 665 563 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2180 2663 2180
Size: 483 6032
BsiEI CG_ry'CG
Cuts at: 104 4057 4103 5333 104
Size: 3953 46 1230 1286
Fragments arranged by size:
3953 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1395 2030 3226 3275 3529 4307 4497 5357 1395
Size: 635 1196 49 254 778 190 860 2553
Fragments arranged by size:
2553 1196 860 778 635 254 190 49
BsmI GAATG_Cn'
Cuts at: 1510 1510
Size: 6515
BsmBI CGTCTCn'nnnn_
Cuts at: 2480 2480
Size: 6515
Bsp1286I G_dGCh'C
Cuts at: 119 1395 1850 2030 2486 2596 3226 3275 3437
Size: 1276 455 180 456 110 630 49 162
Cuts at: 3437 3529 4140 4233 4307 4497 4559 5357 119
Size: 92 611 93 74 190 62 798 1277
Fragments arranged by size:
1277 1276 798 630 611 456 455 190
180 162 110 93 92 74 62 49
BspHI T'CATG_A
Cuts at: 4947 4947
Size: 6515
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2220 3565 3779 4081 4462 2220
Size: 1345 214 302 381 4273
Fragments arranged by size:
4273 1345 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1328 4807 4861 6345 6510 50
Size: 1278 3479 54 1484 165 55
Fragments arranged by size:
3479 1484 1278 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1967 4427 1967
Size: 2460 4055
BsrFI r'CCGG_y
Cuts at: 225 775 2533 3160 4513 4694 225
Size: 550 1758 627 1353 181 2046
Fragments arranged by size:
2046 1758 1353 627 550 181
BsrGI T'GTAC_A
Cuts at: 2239 2239
Size: 6515
BssSI C'ACGA_G
Cuts at: 4786 5494 4786
Size: 708 5807
BstAPI GCAn_nnn'nTGC
Cuts at: 1531 3456 3892 1531
Size: 1925 436 4154
Fragments arranged by size:
4154 1925 436
BstBI TT'CG_AA
Cuts at: 4876 4876
Size: 6515
BstXI CCAn_nnnn'nTGG
Cuts at: 1029 1542 2145 3364 1029
Size: 513 603 1219 4180
Fragments arranged by size:
4180 1219 603 513
BstYI r'GATC_y
Cuts at: 1 363 372 3540 4007 4030 4365 4611 4917
Size: 362 9 3168 467 23 335 246 306
Cuts at: 4917 4929 5015 6067 1
Size: 12 86 1052 449
Fragments arranged by size:
3168 1052 467 449 362 335 306 246
86 23 12 9
Bsu36I CC'TnA_GG
Cuts at: 2691 2691
Size: 6515
BtgI C'CryG_G
Cuts at: 1028 1842 2467 2930 3357 3499 1028
Size: 814 625 463 427 142 4044
Fragments arranged by size:
4044 814 625 463 427 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1486 4465 4646 4809 1486
Size: 2979 181 163 3192
Fragments arranged by size:
3192 2979 181 163
BtsI GCAGTG_nn'
Cuts at: 1022 2188 3758 1022
Size: 1166 1570 3779
Fragments arranged by size:
3779 1570 1166
ClaI AT'CG_AT
Cuts at: 485 524 485
Size: 39 6476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3488 3523 3488
Size: 35 6480
DraI TTT'AAA
Cuts at: 3514 3514
Size: 6515
DraIII CAC_nnn'GTG
Cuts at: 2615 2615
Size: 6515
DrdI GACnn_nn'nnGTC
Cuts at: 3078 4221 5565 3078
Size: 1143 1344 4028
Fragments arranged by size:
4028 1344 1143
EaeI y'GGCC_r
Cuts at: 1019 1487 1865 2651 3158 3366 4100 4274 4665
Size: 468 378 786 507 208 734 174 391
Cuts at: 4665 4692 6037 1019
Size: 27 1345 1497
Fragments arranged by size:
1497 1345 786 734 507 468 391 378
208 174 27
EagI C'GGCC_G
Cuts at: 4100 4100
Size: 6515
EarI CTCTTCn'nnn_
Cuts at: 1164 1541 2751 2865 4538 4748 1164
Size: 377 1210 114 1673 210 2931
Fragments arranged by size:
2931 1673 1210 377 210 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4723 5465 5611 6325 4723
Size: 742 146 714 4913
Fragments arranged by size:
4913 742 714 146
EcoICRI GAG'CTC
Cuts at: 2028 3224 3527 2028
Size: 1196 303 5016
Fragments arranged by size:
5016 1196 303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 368 653 973 997 1061 1210 1426 1450
Size: 43 285 320 24 64 149 216 24
Cuts at: 1450 1720 1735 2098 2572 2694 2999 3264 3378
Size: 270 15 363 474 122 305 265 114
Cuts at: 3378 4340 4772 5125 5787 325
Size: 962 432 353 662 1053
Fragments arranged by size:
1053 962 662 474 432 363 353 320
305 285 270 265 216 149 122 114
64 43 24 24 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2019 2416 2019
Size: 397 6118
EcoO109I rG'GnC_Cy
Cuts at: 414 939 1630 1710 2012 2421 2483 2592 3047
Size: 525 691 80 302 409 62 109 455
Cuts at: 3047 3334 3347 3487 3654 414
Size: 287 13 140 167 3275
Fragments arranged by size:
3275 691 525 455 409 302 287 167
140 109 80 62 13
EcoRI G'AATT_C
Cuts at: 1144 2182 3519 1144
Size: 1038 1337 4140
Fragments arranged by size:
4140 1337 1038
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4534 4534
Size: 6515
FspI TGC'GCA
Cuts at: 4296 4296
Size: 6515
HaeII r_GCGC'y
Cuts at: 3287 3415 3949 4197 5427 6198 3287
Size: 128 534 248 1230 771 3604
Fragments arranged by size:
3604 1230 771 534 248 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 733 765 2017 2049 2094 2126 4357 4387 4389
Size: 32 1252 32 45 32 2231 30 2
Cuts at: 4389 4419 733
Size: 30 2829
Fragments arranged by size:
2829 2231 1252 45 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2073 2999 3554 2073
Size: 926 555 5034
Fragments arranged by size:
5034 926 555
KasI G'GCGC_C
Cuts at: 3945 4193 3945
Size: 248 6267
KpnI G_GTAC'C
Cuts at: 3535 3535
Size: 6515
MluI A'CGCG_T
Cuts at: 5948 5948
Size: 6515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 92 536 2205 5274 5458 92
Size: 444 1669 3069 184 1149
Fragments arranged by size:
3069 1669 1149 444 184
MscI TGG'CCA
Cuts at: 1489 1867 3368 4276 1489
Size: 378 1501 908 3728
Fragments arranged by size:
3728 1501 908 378
MslI CAynn'nnrTG
Cuts at: 1027 1409 4504 4631 1027
Size: 382 3095 127 2911
Fragments arranged by size:
3095 2911 382 127
MspA1I CmG'CkG
Cuts at: 238 928 2170 2650 3626 3940 4300 5084 5329
Size: 690 1242 480 976 314 360 784 245
Cuts at: 5329 238
Size: 1424
Fragments arranged by size:
1424 1242 976 784 690 480 360 314
245
NaeI GCC'GGC
Cuts at: 227 777 2535 3162 4696 227
Size: 550 1758 627 1534 2046
Fragments arranged by size:
2046 1758 1534 627 550
NarI GG'CG_CC
Cuts at: 3946 4194 3946
Size: 248 6267
NcoI C'CATG_G
Cuts at: 1028 1842 3357 1028
Size: 814 1515 4186
Fragments arranged by size:
4186 1515 814
NgoMIV G'CCGG_C
Cuts at: 225 775 2533 3160 4694 225
Size: 550 1758 627 1534 2046
Fragments arranged by size:
2046 1758 1534 627 550
NheI G'CTAG_C
Cuts at: 2293 2293
Size: 6515
NspI r_CATG'y
Cuts at: 3568 4068 5671 3568
Size: 500 1603 4412
Fragments arranged by size:
4412 1603 500
PasI CC'CwG_GG
Cuts at: 3051 3051
Size: 6515
PciI A'CATG_T
Cuts at: 5667 5667
Size: 6515
PflMI CCAn_nnn'nTGG
Cuts at: 1713 1799 3057 1713
Size: 86 1258 5171
Fragments arranged by size:
5171 1258 86
PfoI T'CCnGG_A
Cuts at: 178 178
Size: 6515
PmeI GTTT'AAAC
Cuts at: 3514 3514
Size: 6515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4961 4993 4961
Size: 32 6483
PpuMI rG'GwC_Cy
Cuts at: 1630 1710 3047 3347 1630
Size: 80 1337 300 4798
Fragments arranged by size:
4798 1337 300 80
PsiI TTA'TAA
Cuts at: 3244 3244
Size: 6515
PspOMI G'GGCC_C
Cuts at: 2482 2592 2482
Size: 110 6405
PspXI vC'TCGA_Gb
Cuts at: 2016 2016
Size: 6515
PstI C_TGCA'G
Cuts at: 234 611 1369 2217 2306 2636 2793 3099 3562
Size: 377 758 848 89 330 157 306 463
Cuts at: 3562 4247 234
Size: 685 2502
Fragments arranged by size:
2502 848 758 685 463 377 330 306
157 89
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6515
PvuII CAG'CTG
Cuts at: 238 2170 3940 4300 238
Size: 1932 1770 360 2453
Fragments arranged by size:
2453 1932 1770 360
RsrII CG'GwC_CG
Cuts at: 4710 4710
Size: 6515
SacI G_AGCT'C
Cuts at: 2030 3226 3529 2030
Size: 1196 303 5016
Fragments arranged by size:
5016 1196 303
SalI G'TCGA_C
Cuts at: 3552 3552
Size: 6515
SanDI GG'GwC_CC
Cuts at: 3047 3047
Size: 6515
SapI GCTCTTCn'nnn_
Cuts at: 2865 4538 4748 2865
Size: 1673 210 4632
Fragments arranged by size:
4632 1673 210
SbfI CC_TGCA'GG
Cuts at: 3562 3562
Size: 6515
ScaI AGT'ACT
Cuts at: 6032 6032
Size: 6515
SfcI C'TryA_G
Cuts at: 33 230 607 1365 2213 2302 2632 2789 3095
Size: 197 377 758 848 89 330 157 306
Cuts at: 3095 3558 4243 5211 5402 33
Size: 463 685 968 191 1146
Fragments arranged by size:
1146 968 848 758 685 463 377 330
306 197 191 157 89
SfoI GGC'GCC
Cuts at: 3947 4195 3947
Size: 248 6267
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6515
SmaI CCC'GGG
Cuts at: 3345 3537 3345
Size: 192 6323
SmlI C'TyrA_G
Cuts at: 1384 1450 2016 3670 4585 5299 5561 1384
Size: 66 566 1654 915 714 262 2338
Fragments arranged by size:
2338 1654 915 714 566 262 66
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6515
SphI G_CATG'C
Cuts at: 3568 4068 3568
Size: 500 6015
SspI AAT'ATT
Cuts at: 823 823
Size: 6515
StuI AGG'CCT
Cuts at: 338 1175 2000 2699 338
Size: 837 825 699 4154
Fragments arranged by size:
4154 837 825 699
StyI C'CwwG_G
Cuts at: 1028 1122 1842 2138 3233 3314 3357 3363 3649
Size: 94 720 296 1095 81 43 6 286
Cuts at: 3649 4003 1028
Size: 354 3540
Fragments arranged by size:
3540 1095 720 354 296 286 94 81
43 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 482 1276 3454 4098 4231 4690 4899 482
Size: 794 2178 644 133 459 209 2098
Fragments arranged by size:
2178 2098 794 644 459 209 133
TatI w'GTAC_w
Cuts at: 2239 2433 6030 2239
Size: 194 3597 2724
Fragments arranged by size:
3597 2724 194
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1660 1692 6032 6064 1660
Size: 32 4340 32 2111
Fragments arranged by size:
4340 2111 32 32
Tth111I GACn'n_nGTC
Cuts at: 3114 4312 3114
Size: 1198 5317
XbaI T'CTAG_A
Cuts at: 3546 3546
Size: 6515
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1270 3361 4010 1270
Size: 2091 649 3775
Fragments arranged by size:
3775 2091 649
XhoI C'TCGA_G
Cuts at: 2016 2016
Size: 6515
XmaI C'CCGG_G
Cuts at: 3343 3535 3343
Size: 192 6323
XmnI GAAnn'nnTTC
Cuts at: 1116 1255 1642 2554 1116
Size: 139 387 912 5077
Fragments arranged by size:
5077 912 387 139
ZraI GAC'GTC
Cuts at: 2474 2474
Size: 6515
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AleI AloI AscI AvrII BaeI
BcgI BfrBI BmgBI BsiWI BspEI BssHII BstEII BstZ17I
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI NsiI PacI PmlI PshAI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SwaI