Restriction map for
pF1KB9498
(Circular) (Six-base) MAPSORT of: pF1KB9498.seq Check: 8685 from: 1 to: 4946
pF1KB9498 4946 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1984 1984
Size: 4946
Acc65I G'GTAC_C
Cuts at: 1962 1962
Size: 4946
AclI AA'CG_TT
Cuts at: 4786 4786
Size: 4946
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2771 3203 3556 4218 2771
Size: 432 353 662 3499
Fragments arranged by size:
3499 662 432 353
AflIII A'CryG_T
Cuts at: 2298 4098 4379 2298
Size: 1800 281 2865
Fragments arranged by size:
2865 1800 281
AgeI A'CCGG_T
Cuts at: 800 800
Size: 4946
AlwNI CAG_nnn'CTG
Cuts at: 2251 3689 2251
Size: 1438 3508
ApaLI G'TGCA_C
Cuts at: 3784 3784
Size: 4946
ApoI r'AATT_y
Cuts at: 448 535 602 1950 4299 4555 448
Size: 87 67 1348 2349 256 839
Fragments arranged by size:
2349 1348 839 256 87 67
AseI AT'TA_AT
Cuts at: 20 20
Size: 4946
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4946
AvaI C'yCGr_G
Cuts at: 347 1058 1172 1307 1966 347
Size: 711 114 135 659 3327
Fragments arranged by size:
3327 711 659 135 114
BamHI G'GATC_C
Cuts at: 1971 1971
Size: 4946
BanI G'GyrC_C
Cuts at: 300 1962 2376 2624 2659 300
Size: 1662 414 248 35 2587
Fragments arranged by size:
2587 1662 414 248 35
BanII G_rGCy'C
Cuts at: 711 1042 1600 1960 2990 711
Size: 331 558 360 1030 2667
Fragments arranged by size:
2667 1030 558 360 331
BbeI G_GCGC'C
Cuts at: 304 2380 2628 304
Size: 2076 248 2622
Fragments arranged by size:
2622 2076 248
BbsI GAAGACnn'nnnn_
Cuts at: 151 367 1010 151
Size: 216 643 4087
Fragments arranged by size:
4087 643 216
BbvCI CC'TCA_GC
Cuts at: 117 173 117
Size: 56 4890
BciVI GTATCCnnnnn_n'
Cuts at: 2844 3900 4946 2844
Size: 1056 1046 2844
Fragments arranged by size:
2844 1056 1046
BclI T'GATC_A
Cuts at: 621 1509 2466 621
Size: 888 957 3101
Fragments arranged by size:
3101 957 888
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4946
BglII A'GATC_T
Cuts at: 1819 2461 4498 1819
Size: 642 2037 2267
Fragments arranged by size:
2267 2037 642
BlpI GC'TnA_GC
Cuts at: 2058 2058
Size: 4946
Bme1580I G_kGCm'C
Cuts at: 567 2571 2664 3788 567
Size: 2004 93 1124 1725
Fragments arranged by size:
2004 1725 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1158 1397 1736 2158 2239 2574 4718 1158
Size: 239 339 422 81 335 2144 1386
Fragments arranged by size:
2144 1386 422 339 335 239 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3313 3313
Size: 4946
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 723 1026 1631 1800 723
Size: 303 605 169 3869
Fragments arranged by size:
3869 605 303 169
Bpu10I CC'TnA_GC
Cuts at: 117 173 1829 117
Size: 56 1656 3234
Fragments arranged by size:
3234 1656 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2122 3001 3715 4013 2122
Size: 879 714 298 3055
Fragments arranged by size:
3055 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1760 4650 1760
Size: 2890 2056
BsaAI yAC'GTr
Cuts at: 490 2299 490
Size: 1809 3137
BsaBI GATnn'nnATC
Cuts at: 6 671 815 827 854 2460 4497 6
Size: 665 144 12 27 1606 2037 455
Fragments arranged by size:
2037 1606 665 455 144 27 12
BsaHI Gr'CG_yC
Cuts at: 301 2377 2625 3327 4823 301
Size: 2076 248 702 1496 424
Fragments arranged by size:
2076 1496 702 424 248
BsaWI w'CCGG_w
Cuts at: 365 800 2135 2656 3334 3745 3892 4166 365
Size: 435 1335 521 678 411 147 274 1145
Fragments arranged by size:
1335 1145 678 521 435 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 696 726 1416 1446 696
Size: 30 690 30 4196
Fragments arranged by size:
4196 690 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 998 998
Size: 4946
BseYI C'CCAG_C
Cuts at: 224 496 2371 3129 3794 4202 224
Size: 272 1875 758 665 408 968
Fragments arranged by size:
1875 968 758 665 408 272
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 1808 494
Size: 1314 3632
BsiEI CG_ry'CG
Cuts at: 104 366 1033 1360 2488 2534 3764 104
Size: 262 667 327 1128 46 1230 1286
Fragments arranged by size:
1286 1230 1128 667 327 262 46
BsiHKAI G_wGCw'C
Cuts at: 1042 1600 1960 2738 2928 3788 1042
Size: 558 360 778 190 860 2200
Fragments arranged by size:
2200 860 778 558 360 190
BsmI GAATG_Cn'
Cuts at: 1557 1713 1557
Size: 156 4790
BsmBI CGTCTCn'nnnn_
Cuts at: 1745 1745
Size: 4946
Bsp1286I G_dGCh'C
Cuts at: 567 711 1042 1600 1960 2571 2664 2738 2928
Size: 144 331 558 360 611 93 74 190
Cuts at: 2928 2990 3788 567
Size: 62 798 1725
Fragments arranged by size:
1725 798 611 558 360 331 190 144
93 74 62
BspHI T'CATG_A
Cuts at: 3378 3378
Size: 4946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1996 2210 2512 2893 1996
Size: 214 302 381 4049
Fragments arranged by size:
4049 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3238 3292 4776 4941 50
Size: 3188 54 1484 165 55
Fragments arranged by size:
3188 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2858 2858
Size: 4946
BsrFI r'CCGG_y
Cuts at: 800 2944 3125 800
Size: 2144 181 2621
Fragments arranged by size:
2621 2144 181
BssSI C'ACGA_G
Cuts at: 3217 3925 3217
Size: 708 4238
BstAPI GCAn_nnn'nTGC
Cuts at: 2323 2323
Size: 4946
BstBI TT'CG_AA
Cuts at: 3307 3307
Size: 4946
BstXI CCAn_nnnn'nTGG
Cuts at: 224 224
Size: 4946
BstYI r'GATC_y
Cuts at: 1 1819 1971 2438 2461 2796 3042 3348 3360
Size: 1818 152 467 23 335 246 306 12
Cuts at: 3360 3446 4498 1
Size: 86 1052 449
Fragments arranged by size:
1818 1052 467 449 335 306 246 152
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1133 1844 1133
Size: 711 4235
BtgI C'CryG_G
Cuts at: 107 337 1740 107
Size: 230 1403 3313
Fragments arranged by size:
3313 1403 230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2896 3077 3240 2896
Size: 181 163 4602
Fragments arranged by size:
4602 181 163
BtsI GCAGTG_nn'
Cuts at: 329 395 482 939 960 2189 329
Size: 66 87 457 21 1229 3086
Fragments arranged by size:
3086 1229 457 87 66 21
ClaI AT'CG_AT
Cuts at: 858 858
Size: 4946
DraI TTT'AAA
Cuts at: 1945 1945
Size: 4946
DrdI GACnn_nn'nnGTC
Cuts at: 2652 3996 2652
Size: 1344 3602
EaeI y'GGCC_r
Cuts at: 660 867 2531 2705 3096 3123 4468 660
Size: 207 1664 174 391 27 1345 1138
Fragments arranged by size:
1664 1345 1138 391 207 174 27
EagI C'GGCC_G
Cuts at: 2531 2531
Size: 4946
EarI CTCTTCn'nnn_
Cuts at: 428 575 744 936 2969 3179 428
Size: 147 169 192 2033 210 2195
Fragments arranged by size:
2195 2033 210 192 169 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3154 3896 4042 4756 3154
Size: 742 146 714 3344
Fragments arranged by size:
3344 742 714 146
EcoICRI GAG'CTC
Cuts at: 1040 1598 1958 1040
Size: 558 360 4028
Fragments arranged by size:
4028 558 360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 723 1026 1631 1800 2771 3203 3556 4218 723
Size: 303 605 169 971 432 353 662 1451
Fragments arranged by size:
1451 971 662 605 432 353 303 169
EcoNI CCTnn'n_nnAGG
Cuts at: 749 749
Size: 4946
EcoO109I rG'GnC_Cy
Cuts at: 543 1087 1391 2085 543
Size: 544 304 694 3404
Fragments arranged by size:
3404 694 544 304
EcoRI G'AATT_C
Cuts at: 448 602 1950 448
Size: 154 1348 3444
Fragments arranged by size:
3444 1348 154
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 468 2965 468
Size: 2497 2449
FspI TGC'GCA
Cuts at: 2727 2727
Size: 4946
HaeII r_GCGC'y
Cuts at: 304 2380 2628 3858 4629 304
Size: 2076 248 1230 771 621
Fragments arranged by size:
2076 1230 771 621 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 452 484 696 728 1015 1039 1047 1071 1132
Size: 32 212 32 287 24 8 24 61
Cuts at: 1132 1164 1465 1497 1600 1632 1798 1804 1830
Size: 32 301 32 103 32 166 6 26
Cuts at: 1830 1836 2788 2818 2820 2850 452
Size: 6 952 30 2 30 2548
Fragments arranged by size:
2548 952 301 287 212 166 103 61
32 32 32 32 32 30 30 26
24 24 8 6 6 2
HincII GTy'rAC
Cuts at: 1209 1985 1209
Size: 776 4170
KasI G'GCGC_C
Cuts at: 300 2376 2624 300
Size: 2076 248 2622
Fragments arranged by size:
2622 2076 248
KpnI G_GTAC'C
Cuts at: 1966 1966
Size: 4946
MfeI C'AATT_G
Cuts at: 308 308
Size: 4946
MluI A'CGCG_T
Cuts at: 4379 4379
Size: 4946
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1510 3705 3889 1510
Size: 2195 184 2567
Fragments arranged by size:
2567 2195 184
MscI TGG'CCA
Cuts at: 2707 2707
Size: 4946
MslI CAynn'nnrTG
Cuts at: 2935 3062 2935
Size: 127 4819
MspA1I CmG'CkG
Cuts at: 224 1216 1278 2057 2371 2731 3515 3760 224
Size: 992 62 779 314 360 784 245 1410
Fragments arranged by size:
1410 992 784 779 360 314 245 62
NaeI GCC'GGC
Cuts at: 3127 3127
Size: 4946
NarI GG'CG_CC
Cuts at: 301 2377 2625 301
Size: 2076 248 2622
Fragments arranged by size:
2622 2076 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4946
NgoMIV G'CCGG_C
Cuts at: 3125 3125
Size: 4946
NspI r_CATG'y
Cuts at: 1999 2499 4102 1999
Size: 500 1603 2843
Fragments arranged by size:
2843 1603 500
PciI A'CATG_T
Cuts at: 4098 4098
Size: 4946
PfoI T'CCnGG_A
Cuts at: 922 1746 922
Size: 824 4122
PmeI GTTT'AAAC
Cuts at: 1945 1945
Size: 4946
PmlI CAC'GTG
Cuts at: 490 490
Size: 4946
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3392 3424 3392
Size: 32 4914
PpuMI rG'GwC_Cy
Cuts at: 543 1087 1391 543
Size: 544 304 4098
Fragments arranged by size:
4098 544 304
PspXI vC'TCGA_Gb
Cuts at: 1172 1172
Size: 4946
PstI C_TGCA'G
Cuts at: 1993 2678 1993
Size: 685 4261
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4946
PvuII CAG'CTG
Cuts at: 224 1216 1278 2371 2731 224
Size: 992 62 1093 360 2439
Fragments arranged by size:
2439 1093 992 360 62
RsrII CG'GwC_CG
Cuts at: 3141 3141
Size: 4946
SacI G_AGCT'C
Cuts at: 1042 1600 1960 1042
Size: 558 360 4028
Fragments arranged by size:
4028 558 360
SalI G'TCGA_C
Cuts at: 1983 1983
Size: 4946
SapI GCTCTTCn'nnn_
Cuts at: 936 2969 3179 936
Size: 2033 210 2703
Fragments arranged by size:
2703 2033 210
SbfI CC_TGCA'GG
Cuts at: 1993 1993
Size: 4946
ScaI AGT'ACT
Cuts at: 1128 4463 1128
Size: 3335 1611
SfcI C'TryA_G
Cuts at: 33 719 978 1246 1989 2674 3642 3833 33
Size: 686 259 268 743 685 968 191 1146
Fragments arranged by size:
1146 968 743 686 685 268 259 191
SfoI GGC'GCC
Cuts at: 302 2378 2626 302
Size: 2076 248 2622
Fragments arranged by size:
2622 2076 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4946
SmaI CCC'GGG
Cuts at: 1968 1968
Size: 4946
SmlI C'TyrA_G
Cuts at: 1172 2101 3016 3730 3992 1172
Size: 929 915 714 262 2126
Fragments arranged by size:
2126 929 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4946
SphI G_CATG'C
Cuts at: 1999 2499 1999
Size: 500 4446
StyI C'CwwG_G
Cuts at: 107 2080 2434 107
Size: 1973 354 2619
Fragments arranged by size:
2619 1973 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 902 1019 1022 1334 1548 2529 2662 3121 3330
Size: 117 3 312 214 981 133 459 209
Cuts at: 3330 902
Size: 2518
Fragments arranged by size:
2518 981 459 312 214 209 133 117
3
TatI w'GTAC_w
Cuts at: 1126 4461 1126
Size: 3335 1611
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 918 950 1356 1388 1416 1448 4463 4495 918
Size: 32 406 32 28 32 3015 32 1369
Fragments arranged by size:
3015 1369 406 32 32 32 32 28
Tth111I GACn'n_nGTC
Cuts at: 2743 2743
Size: 4946
XbaI T'CTAG_A
Cuts at: 1443 1977 1443
Size: 534 4412
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2441 2441
Size: 4946
XhoI C'TCGA_G
Cuts at: 1172 1172
Size: 4946
XmaI C'CCGG_G
Cuts at: 1966 1966
Size: 4946
XmnI GAAnn'nnTTC
Cuts at: 452 452
Size: 4946
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PmlI PpiI PpuMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI
BsiWI BspEI BsrGI BssHII BstEII BstZ17I CspCI DraIII
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PshAI PsiI PspOMI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI ZraI