Restriction map for
pF1KB9495
(Circular) (Six-base) MAPSORT of: pF1KB9495.seq Check: 6728 from: 1 to: 5621
pF1KB9495 5621 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 688 1469 688
Size: 781 4840
AccI GT'mk_AC
Cuts at: 1687 2659 1687
Size: 972 4649
Acc65I G'GTAC_C
Cuts at: 2637 2637
Size: 5621
AclI AA'CG_TT
Cuts at: 5461 5461
Size: 5621
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 569 596 1432 1879 2456 3446 3878 4231 4893
Size: 27 836 447 577 990 432 353 662
Cuts at: 4893 569
Size: 1297
Fragments arranged by size:
1297 990 836 662 577 447 432 353
27
AfeI AGC'GCT
Cuts at: 1542 1542
Size: 5621
AflII C'TTAA_G
Cuts at: 1282 1282
Size: 5621
AflIII A'CryG_T
Cuts at: 2973 4773 5054 2973
Size: 1800 281 3540
Fragments arranged by size:
3540 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 569 710 2379 569
Size: 141 1669 3811
Fragments arranged by size:
3811 1669 141
AleI CACnn'nnGTG
Cuts at: 2320 2320
Size: 5621
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1420 1511 1420
Size: 91 5530
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 397 429 397
Size: 32 5589
AlwNI CAG_nnn'CTG
Cuts at: 680 1435 1623 1887 2478 2926 4364 680
Size: 755 188 264 591 448 1438 1937
Fragments arranged by size:
1937 1438 755 591 448 264 188
ApaI G_GGCC'C
Cuts at: 2021 2190 2021
Size: 169 5452
ApaLI G'TGCA_C
Cuts at: 4459 4459
Size: 5621
ApoI r'AATT_y
Cuts at: 1287 2625 4974 5230 1287
Size: 1338 2349 256 1678
Fragments arranged by size:
2349 1678 1338 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5621
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5621
AvaI C'yCGr_G
Cuts at: 161 187 239 1248 1788 2020 2641 161
Size: 26 52 1009 540 232 621 3141
Fragments arranged by size:
3141 1009 621 540 232 52 26
BamHI G'GATC_C
Cuts at: 616 2646 616
Size: 2030 3591
BanI G'GyrC_C
Cuts at: 153 319 331 1404 2637 3051 3299 3334 153
Size: 166 12 1073 1233 414 248 35 2440
Fragments arranged by size:
2440 1233 1073 414 248 166 35 12
BanII G_rGCy'C
Cuts at: 188 949 1248 1741 2021 2190 2635 3665 188
Size: 761 299 493 280 169 445 1030 2144
Fragments arranged by size:
2144 1030 761 493 445 299 280 169
BbeI G_GCGC'C
Cuts at: 157 323 335 3055 3303 157
Size: 166 12 2720 248 2475
Fragments arranged by size:
2720 2475 248 166 12
BbsI GAAGACnn'nnnn_
Cuts at: 788 788
Size: 5621
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 379 413 379
Size: 34 5587
BciVI GTATCCnnnnn_n'
Cuts at: 1313 1956 3519 4575 5621 1313
Size: 643 1563 1056 1046 1313
Fragments arranged by size:
1563 1313 1056 1046 643
BclI T'GATC_A
Cuts at: 3141 3141
Size: 5621
BglI GCCn_nnn'nGGC
Cuts at: 130 192 262 271 796 1430 1655 2266 130
Size: 62 70 9 525 634 225 611 3485
Fragments arranged by size:
3485 634 611 525 225 70 62 9
BglII A'GATC_T
Cuts at: 741 3136 5173 741
Size: 2395 2037 1189
Fragments arranged by size:
2395 2037 1189
BlpI GC'TnA_GC
Cuts at: 1544 2733 1544
Size: 1189 4432
Bme1580I G_kGCm'C
Cuts at: 1409 2021 2190 3246 3339 4463 1409
Size: 612 169 1056 93 1124 2567
Fragments arranged by size:
2567 1124 1056 612 169 93
BmrI ACTGGGnnnn_n'
Cuts at: 385 911 1773 1796 2457 2833 2914 3249 5393
Size: 526 862 23 661 376 81 335 2144
Cuts at: 5393 385
Size: 613
Fragments arranged by size:
2144 862 661 613 526 376 335 81
23
BmtI G_CTAG'C
Cuts at: 2273 2273
Size: 5621
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3988 3988
Size: 5621
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 301 424 580 1774 1836 1864 1909 163
Size: 138 123 156 1194 62 28 45 3875
Fragments arranged by size:
3875 1194 156 138 123 62 45 28
Bpu10I CC'TnA_GC
Cuts at: 582 2114 582
Size: 1532 4089
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 729 1963 2545 2797 3676 4390 4688 729
Size: 1234 582 252 879 714 298 1662
Fragments arranged by size:
1662 1234 879 714 582 298 252
BsaI GGTCTCn'nnnn_
Cuts at: 5325 5325
Size: 5621
BsaAI yAC'GTr
Cuts at: 2265 2974 2265
Size: 709 4912
BsaBI GATnn'nnATC
Cuts at: 6 857 905 3135 5172 6
Size: 851 48 2230 2037 455
Fragments arranged by size:
2230 2037 851 455 48
BsaHI Gr'CG_yC
Cuts at: 154 320 332 1658 1838 3052 3300 4002 5498
Size: 166 12 1326 180 1214 248 702 1496
Cuts at: 5498 154
Size: 277
Fragments arranged by size:
1496 1326 1214 702 277 248 180 166
12
BsaWI w'CCGG_w
Cuts at: 2810 3331 4009 4420 4567 4841 2810
Size: 521 678 411 147 274 3590
Fragments arranged by size:
3590 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 268 298 397 427 1221 1251 1992 2022 268
Size: 30 99 30 794 30 741 30 3867
Fragments arranged by size:
3867 794 741 99 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 421 436 1664 1980 2196 421
Size: 15 1228 316 216 3846
Fragments arranged by size:
3846 1228 316 216 15
BseYI C'CCAG_C
Cuts at: 364 1872 3046 3804 4469 4877 364
Size: 1508 1174 758 665 408 1108
Fragments arranged by size:
1508 1174 1108 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 961
Size: 5621
BsiEI CG_ry'CG
Cuts at: 104 125 2156 2199 2291 3163 3209 4439 104
Size: 21 2031 43 92 872 46 1230 1286
Fragments arranged by size:
2031 1286 1230 872 92 46 43 21
BsiHKAI G_wGCw'C
Cuts at: 557 2419 2635 3413 3603 4463 557
Size: 1862 216 778 190 860 1715
Fragments arranged by size:
1862 1715 860 778 216 190
Bsp1286I G_dGCh'C
Cuts at: 188 557 949 1248 1409 1741 2021 2190 2419
Size: 369 392 299 161 332 280 169 229
Cuts at: 2419 2635 3246 3339 3413 3603 3665 4463 188
Size: 216 611 93 74 190 62 798 1346
Fragments arranged by size:
1346 798 611 392 369 332 299 280
229 216 190 169 161 93 74 62
BspHI T'CATG_A
Cuts at: 4053 4053
Size: 5621
BspMI ACCTGCnnnn'nnnn_
Cuts at: 296 688 1469 2671 2885 3187 3568 296
Size: 392 781 1202 214 302 381 2349
Fragments arranged by size:
2349 1202 781 392 381 302 214
BsrBI CCG'CTC
Cuts at: 50 228 246 3913 3967 5451 5616 50
Size: 178 18 3667 54 1484 165 55
Fragments arranged by size:
3667 1484 178 165 55 54 18
BsrDI GCAATG_nn'
Cuts at: 845 3533 845
Size: 2688 2933
BsrFI r'CCGG_y
Cuts at: 156 2135 3619 3800 156
Size: 1979 1484 181 1977
Fragments arranged by size:
1979 1977 1484 181
BsrGI T'GTAC_A
Cuts at: 1614 1614
Size: 5621
BssSI C'ACGA_G
Cuts at: 3892 4600 3892
Size: 708 4913
BstAPI GCAn_nnn'nTGC
Cuts at: 2998 2998
Size: 5621
BstBI TT'CG_AA
Cuts at: 1291 3982 1291
Size: 2691 2930
BstXI CCAn_nnnn'nTGG
Cuts at: 2322 2322
Size: 5621
BstYI r'GATC_y
Cuts at: 1 616 741 1030 2570 2646 3113 3136 3471
Size: 615 125 289 1540 76 467 23 335
Cuts at: 3471 3717 4023 4035 4121 5173 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1540 1052 615 467 449 335 306 289
246 125 86 76 23 12
Bsu36I CC'TnA_GG
Cuts at: 792 1428 1532 2179 792
Size: 636 104 647 4234
Fragments arranged by size:
4234 647 636 104
BtgI C'CryG_G
Cuts at: 1700 1866 2045 2085 2318 1700
Size: 166 179 40 233 5003
Fragments arranged by size:
5003 233 179 166 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3571 3752 3915 3571
Size: 181 163 5277
Fragments arranged by size:
5277 181 163
BtsI GCAGTG_nn'
Cuts at: 1366 2864 1366
Size: 1498 4123
DraI TTT'AAA
Cuts at: 2620 2620
Size: 5621
DrdI GACnn_nn'nnGTC
Cuts at: 1524 3327 4671 1524
Size: 1803 1344 2474
Fragments arranged by size:
2474 1803 1344
EaeI y'GGCC_r
Cuts at: 122 263 1472 1667 2082 2105 2153 2288 3206
Size: 141 1209 195 415 23 48 135 918
Cuts at: 3206 3380 3771 3798 5143 122
Size: 174 391 27 1345 600
Fragments arranged by size:
1345 1209 918 600 415 391 195 174
141 135 48 27 23
EagI C'GGCC_G
Cuts at: 122 2153 2288 3206 122
Size: 2031 135 918 2537
Fragments arranged by size:
2537 2031 918 135
EarI CTCTTCn'nnn_
Cuts at: 462 545 983 1700 1746 1999 2165 3644 3854
Size: 83 438 717 46 253 166 1479 210
Cuts at: 3854 462
Size: 2229
Fragments arranged by size:
2229 1479 717 438 253 210 166 83
46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 196 289 1497 3829 4571 4717 5431 196
Size: 93 1208 2332 742 146 714 386
Fragments arranged by size:
2332 1208 742 714 386 146 93
EcoICRI GAG'CTC
Cuts at: 2633 2633
Size: 5621
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 301 424 569 580 596 1432 1774 1836
Size: 138 123 145 11 16 836 342 62
Cuts at: 1836 1864 1879 1909 2456 3446 3878 4231 4893
Size: 28 15 30 547 990 432 353 662
Cuts at: 4893 163
Size: 891
Fragments arranged by size:
990 891 836 662 547 432 353 342
145 138 123 62 30 28 16 15
11
EcoNI CCTnn'n_nnAGG
Cuts at: 581 1414 1580 1771 581
Size: 833 166 191 4431
Fragments arranged by size:
4431 833 191 166
EcoO109I rG'GnC_Cy
Cuts at: 1556 1585 2175 2186 2760 1556
Size: 29 590 11 574 4417
Fragments arranged by size:
4417 590 574 29 11
EcoRI G'AATT_C
Cuts at: 1287 2625 1287
Size: 1338 4283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1962 3640 1962
Size: 1678 3943
FspI TGC'GCA
Cuts at: 3402 3402
Size: 5621
HaeII r_GCGC'y
Cuts at: 157 198 300 323 335 1436 1544 3055 3303
Size: 41 102 23 12 1101 108 1511 248
Cuts at: 3303 4533 5304 157
Size: 1230 771 474
Fragments arranged by size:
1511 1230 1101 771 474 248 108 102
41 23 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 555 587 643 675 696 728 775 807 868
Size: 32 56 32 21 32 47 32 61
Cuts at: 868 900 1311 1343 2284 2316 2365 2397 3463
Size: 32 411 32 941 32 49 32 1066
Cuts at: 3463 3493 3495 3525 555
Size: 30 2 30 2651
Fragments arranged by size:
2651 1066 941 411 61 56 49 47
32 32 32 32 32 32 32 32
30 30 21 2
HincII GTy'rAC
Cuts at: 2660 2660
Size: 5621
KasI G'GCGC_C
Cuts at: 153 319 331 3051 3299 153
Size: 166 12 2720 248 2475
Fragments arranged by size:
2720 2475 248 166 12
KpnI G_GTAC'C
Cuts at: 2641 2641
Size: 5621
MfeI C'AATT_G
Cuts at: 448 448
Size: 5621
MluI A'CGCG_T
Cuts at: 5054 5054
Size: 5621
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 737 1466 4380 4564 737
Size: 729 2914 184 1794
Fragments arranged by size:
2914 1794 729 184
MscI TGG'CCA
Cuts at: 2084 2107 3382 2084
Size: 23 1275 4323
Fragments arranged by size:
4323 1275 23
MslI CAynn'nnrTG
Cuts at: 1013 2320 3610 3737 1013
Size: 1307 1290 127 2897
Fragments arranged by size:
2897 1307 1290 127
MspA1I CmG'CkG
Cuts at: 352 1868 2047 2732 3046 3406 4190 4435 352
Size: 1516 179 685 314 360 784 245 1538
Fragments arranged by size:
1538 1516 784 685 360 314 245 179
NaeI GCC'GGC
Cuts at: 158 3802 158
Size: 3644 1977
NarI GG'CG_CC
Cuts at: 154 320 332 3052 3300 154
Size: 166 12 2720 248 2475
Fragments arranged by size:
2720 2475 248 166 12
NgoMIV G'CCGG_C
Cuts at: 156 3800 156
Size: 3644 1977
NheI G'CTAG_C
Cuts at: 2269 2269
Size: 5621
NspI r_CATG'y
Cuts at: 1498 2674 3174 4777 1498
Size: 1176 500 1603 2342
Fragments arranged by size:
2342 1603 1176 500
PciI A'CATG_T
Cuts at: 4773 4773
Size: 5621
PflMI CCAn_nnn'nTGG
Cuts at: 1752 1887 1752
Size: 135 5486
PmeI GTTT'AAAC
Cuts at: 2620 2620
Size: 5621
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 397 429 2290 2322 2386 2418 4067 4099 397
Size: 32 1861 32 64 32 1649 32 1919
Fragments arranged by size:
1919 1861 1649 64 32 32 32 32
PspOMI G'GGCC_C
Cuts at: 2017 2186 2017
Size: 169 5452
PstI C_TGCA'G
Cuts at: 741 1655 2668 3353 741
Size: 914 1013 685 3009
Fragments arranged by size:
3009 1013 914 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5621
PvuII CAG'CTG
Cuts at: 3046 3406 3046
Size: 360 5261
RsrII CG'GwC_CG
Cuts at: 3816 3816
Size: 5621
SacI G_AGCT'C
Cuts at: 2635 2635
Size: 5621
SacII CC_GC'GG
Cuts at: 1869 2048 1869
Size: 179 5442
SalI G'TCGA_C
Cuts at: 2658 2658
Size: 5621
SapI GCTCTTCn'nnn_
Cuts at: 545 1746 3644 3854 545
Size: 1201 1898 210 2312
Fragments arranged by size:
2312 1898 1201 210
SbfI CC_TGCA'GG
Cuts at: 1655 2668 1655
Size: 1013 4608
ScaI AGT'ACT
Cuts at: 2284 5138 2284
Size: 2854 2767
SexAI A'CCwGG_T
Cuts at: 1766 1766
Size: 5621
SfcI C'TryA_G
Cuts at: 33 737 1651 1688 2664 3349 4317 4508 33
Size: 704 914 37 976 685 968 191 1146
Fragments arranged by size:
1146 976 968 914 704 685 191 37
SfiI GGCCn_nnn'nGGCC
Cuts at: 262 262
Size: 5621
SfoI GGC'GCC
Cuts at: 155 321 333 3053 3301 155
Size: 166 12 2720 248 2475
Fragments arranged by size:
2720 2475 248 166 12
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5621
SmaI CCC'GGG
Cuts at: 241 1250 1790 2643 241
Size: 1009 540 853 3219
Fragments arranged by size:
3219 1009 853 540
SmlI C'TyrA_G
Cuts at: 708 1282 1978 2560 2776 3691 4405 4667 708
Size: 574 696 582 216 915 714 262 1662
Fragments arranged by size:
1662 915 714 696 582 574 262 216
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5621
SphI G_CATG'C
Cuts at: 2674 3174 2674
Size: 500 5121
StuI AGG'CCT
Cuts at: 790 1713 2548 790
Size: 923 835 3863
Fragments arranged by size:
3863 923 835
StyI C'CwwG_G
Cuts at: 695 1718 1994 2755 3109 695
Size: 1023 276 761 354 3207
Fragments arranged by size:
3207 1023 761 354 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2573 3204 3337 3796 4005 2573
Size: 631 133 459 209 4189
Fragments arranged by size:
4189 631 459 209 133
TatI w'GTAC_w
Cuts at: 1614 2282 5136 1614
Size: 668 2854 2099
Fragments arranged by size:
2854 2099 668
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1221 1253 1992 2024 5138 5170 1221
Size: 32 739 32 3114 32 1672
Fragments arranged by size:
3114 1672 739 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3418 3418
Size: 5621
XbaI T'CTAG_A
Cuts at: 2652 2652
Size: 5621
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1082 1116 1358 3116 1082
Size: 34 242 1758 3587
Fragments arranged by size:
3587 1758 242 34
XmaI C'CCGG_G
Cuts at: 239 1248 1788 2641 239
Size: 1009 540 853 3219
Fragments arranged by size:
3219 1009 853 540
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NspI PciI PflMI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AgeI AscI AvrII BaeI BbvCI BfrBI BmgBI
BsiWI BsmI BsmBI BspEI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI NcoI
NdeI NotI NruI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspXI PsrI SanDI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI