Restriction map for
pF1KB9492
(Circular) (Six-base) MAPSORT of: pF1KB9492.seq Check: 7623 from: 1 to: 6722
pF1KB9492 6722 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 1569 3760 1569
Size: 2191 4531
Acc65I G'GTAC_C
Cuts at: 3738 3738
Size: 6722
AclI AA'CG_TT
Cuts at: 6562 6562
Size: 6722
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 516 1284 1580 3610 4547 4979 5332 5994 516
Size: 768 296 2030 937 432 353 662 1244
Fragments arranged by size:
2030 1244 937 768 662 432 353 296
AflII C'TTAA_G
Cuts at: 265 265
Size: 6722
AflIII A'CryG_T
Cuts at: 4074 5874 6155 4074
Size: 1800 281 4641
Fragments arranged by size:
4641 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3311 3343 3311
Size: 32 6690
AlwNI CAG_nnn'CTG
Cuts at: 3588 3699 4027 5465 3588
Size: 111 328 1438 4845
Fragments arranged by size:
4845 1438 328 111
ApaLI G'TGCA_C
Cuts at: 2132 5560 2132
Size: 3428 3294
ApoI r'AATT_y
Cuts at: 366 776 1528 1653 1768 1801 2828 3726 6075
Size: 410 752 125 115 33 1027 898 2349
Cuts at: 6075 6331 366
Size: 256 757
Fragments arranged by size:
2349 1027 898 757 752 410 256 125
115 33
AseI AT'TA_AT
Cuts at: 20 955 20
Size: 935 5787
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6722
AvaI C'yCGr_G
Cuts at: 3742 3742
Size: 6722
BamHI G'GATC_C
Cuts at: 1143 3747 1143
Size: 2604 4118
BanI G'GyrC_C
Cuts at: 3738 4152 4400 4435 3738
Size: 414 248 35 6025
Fragments arranged by size:
6025 414 248 35
BanII G_rGCy'C
Cuts at: 264 1692 3736 4766 264
Size: 1428 2044 1030 2220
Fragments arranged by size:
2220 2044 1428 1030
BbeI G_GCGC'C
Cuts at: 4156 4404 4156
Size: 248 6474
BbsI GAAGACnn'nnnn_
Cuts at: 935 1381 2081 2639 2971 935
Size: 446 700 558 332 4686
Fragments arranged by size:
4686 700 558 446 332
BbvCI CC'TCA_GC
Cuts at: 2276 2276
Size: 6722
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2988 3022 2988
Size: 34 6688
BciVI GTATCCnnnnn_n'
Cuts at: 1922 4620 5676 6722 1922
Size: 2698 1056 1046 1922
Fragments arranged by size:
2698 1922 1056 1046
BclI T'GATC_A
Cuts at: 4242 4242
Size: 6722
BfrBI ATG'CAT
Cuts at: 983 2034 3191 983
Size: 1051 1157 4514
Fragments arranged by size:
4514 1157 1051
BglII A'GATC_T
Cuts at: 3114 4237 6274 3114
Size: 1123 2037 3562
Fragments arranged by size:
3562 2037 1123
BlpI GC'TnA_GC
Cuts at: 599 3252 3834 599
Size: 2653 582 3487
Fragments arranged by size:
3487 2653 582
Bme1580I G_kGCm'C
Cuts at: 2136 2850 4347 4440 5564 2136
Size: 714 1497 93 1124 3294
Fragments arranged by size:
3294 1497 1124 714 93
BmrI ACTGGGnnnn_n'
Cuts at: 665 3934 4015 4350 6494 665
Size: 3269 81 335 2144 893
Fragments arranged by size:
3269 2144 893 335 81
BmtI G_CTAG'C
Cuts at: 2236 2236
Size: 6722
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5089 5089
Size: 6722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 257 2977 178
Size: 79 2720 3923
Fragments arranged by size:
3923 2720 79
Bpu10I CC'TnA_GC
Cuts at: 2276 2276
Size: 6722
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1645 2315 3241 3898 4777 5491 5789 1645
Size: 670 926 657 879 714 298 2578
Fragments arranged by size:
2578 926 879 714 670 657 298
BsaI GGTCTCn'nnnn_
Cuts at: 1888 6426 1888
Size: 4538 2184
BsaAI yAC'GTr
Cuts at: 4075 4075
Size: 6722
BsaBI GATnn'nnATC
Cuts at: 6 2504 4236 6273 6
Size: 2498 1732 2037 455
Fragments arranged by size:
2498 2037 1732 455
BsaHI Gr'CG_yC
Cuts at: 4153 4401 5103 6599 4153
Size: 248 702 1496 4276
Fragments arranged by size:
4276 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2888 3911 4432 5110 5521 5668 5942 2888
Size: 1023 521 678 411 147 274 3668
Fragments arranged by size:
3668 1023 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 491 521 677 707 3311 3341 491
Size: 30 156 30 2604 30 3872
Fragments arranged by size:
3872 2604 156 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 230 233 3233 3236 3316 230
Size: 3 3000 3 80 3636
Fragments arranged by size:
3636 3000 80 3 3
BseYI C'CCAG_C
Cuts at: 162 789 3449 4147 4905 5570 5978 162
Size: 627 2660 698 758 665 408 906
Fragments arranged by size:
2660 906 758 698 665 627 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2816 2933 2816
Size: 117 6605
BsiEI CG_ry'CG
Cuts at: 104 4264 4310 5540 104
Size: 4160 46 1230 1286
Fragments arranged by size:
4160 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2136 3736 4514 4704 5564 2136
Size: 1600 778 190 860 3294
Fragments arranged by size:
3294 1600 860 778 190
BsmI GAATG_Cn'
Cuts at: 335 558 3039 335
Size: 223 2481 4018
Fragments arranged by size:
4018 2481 223
Bsp1286I G_dGCh'C
Cuts at: 264 1692 2136 2850 3736 4347 4440 4514 4704
Size: 1428 444 714 886 611 93 74 190
Cuts at: 4704 4766 5564 264
Size: 62 798 1422
Fragments arranged by size:
1428 1422 886 798 714 611 444 190
93 74 62
BspEI T'CCGG_A
Cuts at: 2888 2888
Size: 6722
BspHI T'CATG_A
Cuts at: 5154 5154
Size: 6722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3404 3540 3772 3986 4288 4669 3404
Size: 136 232 214 302 381 5457
Fragments arranged by size:
5457 381 302 232 214 136
BsrBI CCG'CTC
Cuts at: 50 5014 5068 6552 6717 50
Size: 4964 54 1484 165 55
Fragments arranged by size:
4964 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1478 1488 2243 4634 1478
Size: 10 755 2391 3566
Fragments arranged by size:
3566 2391 755 10
BsrFI r'CCGG_y
Cuts at: 4720 4901 4720
Size: 181 6541
BsrGI T'GTAC_A
Cuts at: 407 2082 407
Size: 1675 5047
BssSI C'ACGA_G
Cuts at: 4993 5701 4993
Size: 708 6014
BstAPI GCAn_nnn'nTGC
Cuts at: 1609 4099 1609
Size: 2490 4232
BstBI TT'CG_AA
Cuts at: 5083 5083
Size: 6722
BstXI CCAn_nnnn'nTGG
Cuts at: 728 3487 728
Size: 2759 3963
BstYI r'GATC_y
Cuts at: 1 1114 1143 3114 3747 4214 4237 4572 4818
Size: 1113 29 1971 633 467 23 335 246
Cuts at: 4818 5124 5136 5222 6274 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1971 1113 1052 633 467 449 335 306
246 86 29 23 12
BtgI C'CryG_G
Cuts at: 107 1206 2717 107
Size: 1099 1511 4112
Fragments arranged by size:
4112 1511 1099
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4672 4853 5016 4672
Size: 181 163 6378
Fragments arranged by size:
6378 181 163
BtsI GCAGTG_nn'
Cuts at: 465 499 1084 3965 465
Size: 34 585 2881 3222
Fragments arranged by size:
3222 2881 585 34
DraI TTT'AAA
Cuts at: 1323 3341 3721 1323
Size: 2018 380 4324
Fragments arranged by size:
4324 2018 380
DrdI GACnn_nn'nnGTC
Cuts at: 4428 5772 4428
Size: 1344 5378
EaeI y'GGCC_r
Cuts at: 2015 3383 4307 4481 4872 4899 6244 2015
Size: 1368 924 174 391 27 1345 2493
Fragments arranged by size:
2493 1368 1345 924 391 174 27
EagI C'GGCC_G
Cuts at: 4307 4307
Size: 6722
EarI CTCTTCn'nnn_
Cuts at: 1471 2158 2507 3289 4745 4955 1471
Size: 687 349 782 1456 210 3238
Fragments arranged by size:
3238 1456 782 687 349 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3155 4930 5672 5818 6532 3155
Size: 1775 742 146 714 3345
Fragments arranged by size:
3345 1775 742 714 146
EcoICRI GAG'CTC
Cuts at: 3734 3734
Size: 6722
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 257 516 1284 1580 2977 3610 4547 4979
Size: 79 259 768 296 1397 633 937 432
Cuts at: 4979 5332 5994 178
Size: 353 662 906
Fragments arranged by size:
1397 937 906 768 662 633 432 353
296 259 79
EcoNI CCTnn'n_nnAGG
Cuts at: 1322 1322
Size: 6722
EcoO109I rG'GnC_Cy
Cuts at: 3233 3612 3861 3233
Size: 379 249 6094
Fragments arranged by size:
6094 379 249
EcoRI G'AATT_C
Cuts at: 366 1801 2828 3726 366
Size: 1435 1027 898 3362
Fragments arranged by size:
3362 1435 1027 898
EcoRV GAT'ATC
Cuts at: 2319 2319
Size: 6722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1368 1821 2999 4741 1368
Size: 453 1178 1742 3349
Fragments arranged by size:
3349 1742 1178 453
FspI TGC'GCA
Cuts at: 4503 4503
Size: 6722
HaeII r_GCGC'y
Cuts at: 2112 4156 4404 5634 6405 2112
Size: 2044 248 1230 771 2429
Fragments arranged by size:
2429 2044 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 267 299 1158 1190 1829 1861 2019 2051 4564
Size: 32 859 32 639 32 158 32 2513
Cuts at: 4564 4594 4596 4626 267
Size: 30 2 30 2363
Fragments arranged by size:
2513 2363 859 639 158 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 207 3761 207
Size: 3554 3168
HindIII A'AGCT_T
Cuts at: 712 712
Size: 6722
KasI G'GCGC_C
Cuts at: 4152 4400 4152
Size: 248 6474
KpnI G_GTAC'C
Cuts at: 3742 3742
Size: 6722
MfeI C'AATT_G
Cuts at: 2041 3049 2041
Size: 1008 5714
MluI A'CGCG_T
Cuts at: 6155 6155
Size: 6722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3303 5481 5665 3303
Size: 2178 184 4360
Fragments arranged by size:
4360 2178 184
MscI TGG'CCA
Cuts at: 2017 3385 4483 2017
Size: 1368 1098 4256
Fragments arranged by size:
4256 1368 1098
MslI CAynn'nnrTG
Cuts at: 581 726 978 4711 4838 581
Size: 145 252 3733 127 2465
Fragments arranged by size:
3733 2465 252 145 127
MspA1I CmG'CkG
Cuts at: 257 1606 2215 2808 3453 3833 4147 4507 5291
Size: 1349 609 593 645 380 314 360 784
Cuts at: 5291 5536 257
Size: 245 1443
Fragments arranged by size:
1443 1349 784 645 609 593 380 360
314 245
NaeI GCC'GGC
Cuts at: 4903 4903
Size: 6722
NarI GG'CG_CC
Cuts at: 4153 4401 4153
Size: 248 6474
NcoI C'CATG_G
Cuts at: 107 1206 2717 107
Size: 1099 1511 4112
Fragments arranged by size:
4112 1511 1099
NdeI CA'TA_TG
Cuts at: 3105 3105
Size: 6722
NgoMIV G'CCGG_C
Cuts at: 4901 4901
Size: 6722
NheI G'CTAG_C
Cuts at: 2232 2232
Size: 6722
NsiI A_TGCA'T
Cuts at: 985 2036 3193 985
Size: 1051 1157 4514
Fragments arranged by size:
4514 1157 1051
NspI r_CATG'y
Cuts at: 789 1967 2247 2920 3003 3775 4275 5878 789
Size: 1178 280 673 83 772 500 1603 1633
Fragments arranged by size:
1633 1603 1178 772 673 500 280 83
PciI A'CATG_T
Cuts at: 5874 5874
Size: 6722
PfoI T'CCnGG_A
Cuts at: 1784 3479 1784
Size: 1695 5027
PmeI GTTT'AAAC
Cuts at: 3721 3721
Size: 6722
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3311 3343 5168 5200 3311
Size: 32 1825 32 4833
Fragments arranged by size:
4833 1825 32 32
PpuMI rG'GwC_Cy
Cuts at: 3233 3233
Size: 6722
PshAI GACnn'nnGTC
Cuts at: 968 968
Size: 6722
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3385 3417 3385
Size: 32 6690
PstI C_TGCA'G
Cuts at: 256 2124 2220 2807 3769 4454 256
Size: 1868 96 587 962 685 2524
Fragments arranged by size:
2524 1868 962 685 587 96
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6722
PvuII CAG'CTG
Cuts at: 1606 2215 2808 3453 4147 4507 1606
Size: 609 593 645 694 360 3821
Fragments arranged by size:
3821 694 645 609 593 360
RsrII CG'GwC_CG
Cuts at: 4917 4917
Size: 6722
SacI G_AGCT'C
Cuts at: 3736 3736
Size: 6722
SalI G'TCGA_C
Cuts at: 3759 3759
Size: 6722
SapI GCTCTTCn'nnn_
Cuts at: 1471 4745 4955 1471
Size: 3274 210 3238
Fragments arranged by size:
3274 3238 210
SbfI CC_TGCA'GG
Cuts at: 3769 3769
Size: 6722
ScaI AGT'ACT
Cuts at: 6239 6239
Size: 6722
SexAI A'CCwGG_T
Cuts at: 3506 3506
Size: 6722
SfcI C'TryA_G
Cuts at: 33 252 1570 2120 2216 2803 3645 3765 4450
Size: 219 1318 550 96 587 842 120 685
Cuts at: 4450 5418 5609 33
Size: 968 191 1146
Fragments arranged by size:
1318 1146 968 842 685 587 550 219
191 120 96
SfoI GGC'GCC
Cuts at: 4154 4402 4154
Size: 248 6474
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6722
SmaI CCC'GGG
Cuts at: 3744 3744
Size: 6722
SmlI C'TyrA_G
Cuts at: 265 1660 2330 3220 3877 4792 5506 5768 265
Size: 1395 670 890 657 915 714 262 1219
Fragments arranged by size:
1395 1219 915 890 714 670 657 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6722
SphI G_CATG'C
Cuts at: 3775 4275 3775
Size: 500 6222
SspI AAT'ATT
Cuts at: 329 483 2617 329
Size: 154 2134 4434
Fragments arranged by size:
4434 2134 154
StuI AGG'CCT
Cuts at: 1328 1328
Size: 6722
StyI C'CwwG_G
Cuts at: 107 320 1206 1495 2717 2732 2742 3615 3666
Size: 213 886 289 1222 15 10 873 51
Cuts at: 3666 3856 4210 107
Size: 190 354 2619
Fragments arranged by size:
2619 1222 886 873 354 289 213 190
51 15 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 781 1121 4305 4438 4897 5106 781
Size: 340 3184 133 459 209 2397
Fragments arranged by size:
3184 2397 459 340 209 133
TatI w'GTAC_w
Cuts at: 407 498 546 2082 2531 6237 407
Size: 91 48 1536 449 3706 892
Fragments arranged by size:
3706 1536 892 449 91 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2430 2462 2819 2851 6239 6271 2430
Size: 32 357 32 3388 32 2881
Fragments arranged by size:
3388 2881 357 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4519 4519
Size: 6722
XbaI T'CTAG_A
Cuts at: 373 1017 3753 373
Size: 644 2736 3342
Fragments arranged by size:
3342 2736 644
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4217 4217
Size: 6722
XmaI C'CCGG_G
Cuts at: 3742 3742
Size: 6722
XmnI GAAnn'nnTTC
Cuts at: 383 1521 383
Size: 1138 5584
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NsiI NspI PciI PfoI
PmeI PpiI PpuMI PshAI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI ApaI
AscI AvrII BaeI BglI BmgBI BsiWI BsmBI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII FseI FspAI
HpaI NotI NruI PacI PasI PflMI PmlI PsiI
PspOMI PspXI SacII SanDI SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI