Restriction map for
pF1KB9491
(Circular) (Six-base) MAPSORT of: pF1KB9491.seq Check: 9320 from: 1 to: 6533
pF1KB9491 6533 bp
With 182 enzymes: SgfI *
June 20, 2008 10:31 ..
AccI GT'mk_AC
Cuts at: 3571 3571
Size: 6533
Acc65I G'GTAC_C
Cuts at: 3549 3549
Size: 6533
AclI AA'CG_TT
Cuts at: 1218 6373 1218
Size: 5155 1378
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1069 1220 1819 1840 2500 4358 4790 5143 5805
Size: 151 599 21 660 1858 432 353 662
Cuts at: 5805 1069
Size: 1797
Fragments arranged by size:
1858 1797 662 660 599 432 353 151
21
AflIII A'CryG_T
Cuts at: 2358 3885 5685 5966 2358
Size: 1527 1800 281 2925
Fragments arranged by size:
2925 1800 1527 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 323 548 323
Size: 225 6308
AlwNI CAG_nnn'CTG
Cuts at: 2217 3838 5276 2217
Size: 1621 1438 3474
Fragments arranged by size:
3474 1621 1438
ApaI G_GGCC'C
Cuts at: 3434 3434
Size: 6533
ApaLI G'TGCA_C
Cuts at: 5371 5371
Size: 6533
ApoI r'AATT_y
Cuts at: 163 344 353 490 598 976 2821 2837 3537
Size: 181 9 137 108 378 1845 16 700
Cuts at: 3537 5886 6142 163
Size: 2349 256 554
Fragments arranged by size:
2349 1845 700 554 378 256 181 137
108 16 9
AseI AT'TA_AT
Cuts at: 20 3230 20
Size: 3210 3323
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6533
AvaI C'yCGr_G
Cuts at: 117 3553 117
Size: 3436 3097
BamHI G'GATC_C
Cuts at: 3351 3558 3351
Size: 207 6326
BanI G'GyrC_C
Cuts at: 3549 3963 4211 4246 3549
Size: 414 248 35 5836
Fragments arranged by size:
5836 414 248 35
BanII G_rGCy'C
Cuts at: 2412 3434 3547 4577 2412
Size: 1022 113 1030 4368
Fragments arranged by size:
4368 1030 1022 113
BbeI G_GCGC'C
Cuts at: 3967 4215 3967
Size: 248 6285
BbsI GAAGACnn'nnnn_
Cuts at: 677 1054 1739 1825 2691 677
Size: 377 685 86 866 4519
Fragments arranged by size:
4519 866 685 377 86
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 231 265 231
Size: 34 6499
BciVI GTATCCnnnnn_n'
Cuts at: 4431 5487 6533 4431
Size: 1056 1046 4431
Fragments arranged by size:
4431 1056 1046
BclI T'GATC_A
Cuts at: 4053 4053
Size: 6533
BglII A'GATC_T
Cuts at: 1239 2181 2718 4048 6085 1239
Size: 942 537 1330 2037 1687
Fragments arranged by size:
2037 1687 1330 942 537
BlpI GC'TnA_GC
Cuts at: 3242 3645 3242
Size: 403 6130
Bme1580I G_kGCm'C
Cuts at: 203 2805 3434 4158 4251 5375 203
Size: 2602 629 724 93 1124 1361
Fragments arranged by size:
2602 1361 1124 724 629 93
BmrI ACTGGGnnnn_n'
Cuts at: 2229 2262 3745 3826 4161 6305 2229
Size: 33 1483 81 335 2144 2457
Fragments arranged by size:
2457 2144 1483 335 81 33
BmtI G_CTAG'C
Cuts at: 1204 1204
Size: 6533
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1367 3470 4900 1367
Size: 2103 1430 3000
Fragments arranged by size:
3000 2103 1430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1453 2239 3353 3455 3467 1453
Size: 786 1114 102 12 4519
Fragments arranged by size:
4519 1114 786 102 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 829 2977 3192 3709 4588 5302 5600 829
Size: 2148 215 517 879 714 298 1762
Fragments arranged by size:
2148 1762 879 714 517 298 215
BsaI GGTCTCn'nnnn_
Cuts at: 1888 3110 6237 1888
Size: 1222 3127 2184
Fragments arranged by size:
3127 2184 1222
BsaAI yAC'GTr
Cuts at: 3886 3886
Size: 6533
BsaBI GATnn'nnATC
Cuts at: 6 1688 4047 6084 6
Size: 1682 2359 2037 455
Fragments arranged by size:
2359 2037 1682 455
BsaHI Gr'CG_yC
Cuts at: 3964 4212 4914 6410 3964
Size: 248 702 1496 4087
Fragments arranged by size:
4087 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2492 3722 4243 4921 5332 5479 5753 2492
Size: 1230 521 678 411 147 274 3272
Fragments arranged by size:
3272 1230 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 775 805 775
Size: 30 6503
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 421 826 1531 2332 421
Size: 405 705 801 4622
Fragments arranged by size:
4622 801 705 405
BseYI C'CCAG_C
Cuts at: 196 254 542 3491 3958 4716 5381 5789 196
Size: 58 288 2949 467 758 665 408 940
Fragments arranged by size:
2949 940 758 665 467 408 288 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 554
Size: 6533
BsiEI CG_ry'CG
Cuts at: 104 4075 4121 5351 104
Size: 3971 46 1230 1286
Fragments arranged by size:
3971 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2412 2702 3309 3547 4325 4515 5375 2412
Size: 290 607 238 778 190 860 3570
Fragments arranged by size:
3570 860 778 607 290 238 190
BsmI GAATG_Cn'
Cuts at: 1274 2436 1274
Size: 1162 5371
Bsp1286I G_dGCh'C
Cuts at: 203 2412 2702 2805 3309 3434 3547 4158 4251
Size: 2209 290 103 504 125 113 611 93
Cuts at: 4251 4325 4515 4577 5375 203
Size: 74 190 62 798 1361
Fragments arranged by size:
2209 1361 798 611 504 290 190 125
113 103 93 74 62
BspEI T'CCGG_A
Cuts at: 2492 2492
Size: 6533
BspHI T'CATG_A
Cuts at: 333 2787 2924 4965 333
Size: 2454 137 2041 1901
Fragments arranged by size:
2454 2041 1901 137
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1505 3583 3797 4099 4480 1505
Size: 2078 214 302 381 3558
Fragments arranged by size:
3558 2078 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4825 4879 6363 6528 50
Size: 4775 54 1484 165 55
Fragments arranged by size:
4775 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2114 3336 4445 2114
Size: 1222 1109 4202
Fragments arranged by size:
4202 1222 1109
BsrFI r'CCGG_y
Cuts at: 177 4531 4712 177
Size: 4354 181 1998
Fragments arranged by size:
4354 1998 181
BssSI C'ACGA_G
Cuts at: 4804 5512 4804
Size: 708 5825
BstAPI GCAn_nnn'nTGC
Cuts at: 2259 3910 2259
Size: 1651 4882
BstBI TT'CG_AA
Cuts at: 4894 4894
Size: 6533
BstXI CCAn_nnnn'nTGG
Cuts at: 2817 2817
Size: 6533
BstYI r'GATC_y
Cuts at: 1 1239 2181 2718 3351 3486 3558 4025 4048
Size: 1238 942 537 633 135 72 467 23
Cuts at: 4048 4383 4629 4935 4947 5033 6085 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1238 1052 942 633 537 467 449 335
306 246 135 86 72 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 6533
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4483 4664 4827 4483
Size: 181 163 6189
Fragments arranged by size:
6189 181 163
BtsI GCAGTG_nn'
Cuts at: 3776 3776
Size: 6533
DraI TTT'AAA
Cuts at: 281 2894 3532 281
Size: 2613 638 3282
Fragments arranged by size:
3282 2613 638
DrdI GACnn_nn'nnGTC
Cuts at: 4239 5583 4239
Size: 1344 5189
EaeI y'GGCC_r
Cuts at: 158 179 1764 4118 4292 4683 4710 6055 158
Size: 21 1585 2354 174 391 27 1345 636
Fragments arranged by size:
2354 1585 1345 636 391 174 27 21
EagI C'GGCC_G
Cuts at: 4118 4118
Size: 6533
EarI CTCTTCn'nnn_
Cuts at: 578 1355 1400 1508 1673 1976 2063 2084 2378
Size: 777 45 108 165 303 87 21 294
Cuts at: 2378 2528 4556 4766 578
Size: 150 2028 210 2345
Fragments arranged by size:
2345 2028 777 303 294 210 165 150
108 87 45 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4741 5483 5629 6343 4741
Size: 742 146 714 4931
Fragments arranged by size:
4931 742 714 146
EcoICRI GAG'CTC
Cuts at: 2410 3545 2410
Size: 1135 5398
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1069 1220 1453 1819 1840 2239 2500 3353 3455
Size: 151 233 366 21 399 261 853 102
Cuts at: 3455 3467 4358 4790 5143 5805 1069
Size: 12 891 432 353 662 1797
Fragments arranged by size:
1797 891 853 662 432 399 366 353
261 233 151 102 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1678 1678
Size: 6533
EcoO109I rG'GnC_Cy
Cuts at: 3430 3672 3430
Size: 242 6291
EcoRI G'AATT_C
Cuts at: 3537 3537
Size: 6533
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 982 3121 4552 982
Size: 2139 1431 2963
Fragments arranged by size:
2963 2139 1431
FspI TGC'GCA
Cuts at: 4314 4314
Size: 6533
HaeII r_GCGC'y
Cuts at: 3498 3967 4215 5445 6216 3498
Size: 469 248 1230 771 3815
Fragments arranged by size:
3815 1230 771 469 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 775 807 1303 1333 1335 1365 2022
Size: 32 454 32 496 30 2 30 657
Cuts at: 2022 2054 2086 2118 2437 2469 3107 3139 4375
Size: 32 32 32 319 32 638 32 1236
Cuts at: 4375 4405 4407 4437 289
Size: 30 2 30 2385
Fragments arranged by size:
2385 1236 657 638 496 454 319 32
32 32 32 32 32 32 30 30
30 30 2 2
HincII GTy'rAC
Cuts at: 3572 3572
Size: 6533
HindIII A'AGCT_T
Cuts at: 497 1081 1232 3464 497
Size: 584 151 2232 3566
Fragments arranged by size:
3566 2232 584 151
KasI G'GCGC_C
Cuts at: 3963 4211 3963
Size: 248 6285
KpnI G_GTAC'C
Cuts at: 3553 3553
Size: 6533
MfeI C'AATT_G
Cuts at: 1586 1961 2603 1586
Size: 375 642 5516
Fragments arranged by size:
5516 642 375
MluI A'CGCG_T
Cuts at: 5966 5966
Size: 6533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 576 2897 5292 5476 576
Size: 2321 2395 184 1633
Fragments arranged by size:
2395 2321 1633 184
MscI TGG'CCA
Cuts at: 1766 4294 1766
Size: 2528 4005
MslI CAynn'nnrTG
Cuts at: 466 1604 2591 2786 4522 4649 466
Size: 1138 987 195 1736 127 2350
Fragments arranged by size:
2350 1736 1138 987 195 127
MspA1I CmG'CkG
Cuts at: 192 1207 2289 2599 2644 3162 3644 3958 4318
Size: 1015 1082 310 45 518 482 314 360
Cuts at: 4318 5102 5347 192
Size: 784 245 1378
Fragments arranged by size:
1378 1082 1015 784 518 482 360 314
310 245 45
NaeI GCC'GGC
Cuts at: 179 4714 179
Size: 4535 1998
NarI GG'CG_CC
Cuts at: 3964 4212 3964
Size: 248 6285
NcoI C'CATG_G
Cuts at: 107 107
Size: 6533
NdeI CA'TA_TG
Cuts at: 1580 2704 1580
Size: 1124 5409
NgoMIV G'CCGG_C
Cuts at: 177 4712 177
Size: 4535 1998
NheI G'CTAG_C
Cuts at: 1200 1200
Size: 6533
NspI r_CATG'y
Cuts at: 525 550 2362 2596 3586 4086 5689 525
Size: 25 1812 234 990 500 1603 1369
Fragments arranged by size:
1812 1603 1369 990 500 234 25
PciI A'CATG_T
Cuts at: 2358 5685 2358
Size: 3327 3206
PflMI CCAn_nnn'nTGG
Cuts at: 1398 1779 1398
Size: 381 6152
PmeI GTTT'AAAC
Cuts at: 3532 3532
Size: 6533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4979 5011 4979
Size: 32 6501
PsiI TTA'TAA
Cuts at: 503 503
Size: 6533
PspOMI G'GGCC_C
Cuts at: 3430 3430
Size: 6533
PstI C_TGCA'G
Cuts at: 1815 2445 3580 4265 1815
Size: 630 1135 685 4083
Fragments arranged by size:
4083 1135 685 630
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6533
PvuII CAG'CTG
Cuts at: 192 1207 2599 2644 3162 3958 4318 192
Size: 1015 1392 45 518 796 360 2407
Fragments arranged by size:
2407 1392 1015 796 518 360 45
RsrII CG'GwC_CG
Cuts at: 4728 4728
Size: 6533
SacI G_AGCT'C
Cuts at: 2412 3547 2412
Size: 1135 5398
SalI G'TCGA_C
Cuts at: 3570 3570
Size: 6533
SapI GCTCTTCn'nnn_
Cuts at: 1400 4556 4766 1400
Size: 3156 210 3167
Fragments arranged by size:
3167 3156 210
SbfI CC_TGCA'GG
Cuts at: 3580 3580
Size: 6533
ScaI AGT'ACT
Cuts at: 1727 6050 1727
Size: 4323 2210
SfcI C'TryA_G
Cuts at: 33 213 937 1010 1811 2441 3576 4261 5229
Size: 180 724 73 801 630 1135 685 968
Cuts at: 5229 5420 33
Size: 191 1146
Fragments arranged by size:
1146 1135 968 801 724 685 630 191
180 73
SfoI GGC'GCC
Cuts at: 3965 4213 3965
Size: 248 6285
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6533
SmaI CCC'GGG
Cuts at: 119 3555 119
Size: 3436 3097
SmlI C'TyrA_G
Cuts at: 808 2992 3207 3688 4603 5317 5579 808
Size: 2184 215 481 915 714 262 1762
Fragments arranged by size:
2184 1762 915 714 481 262 215
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6533
SphI G_CATG'C
Cuts at: 3586 4086 3586
Size: 500 6033
SspI AAT'ATT
Cuts at: 380 1474 380
Size: 1094 5439
StyI C'CwwG_G
Cuts at: 107 263 1148 1898 3667 4021 107
Size: 156 885 750 1769 354 2619
Fragments arranged by size:
2619 1769 885 750 354 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4116 4249 4708 4917 4116
Size: 133 459 209 5732
Fragments arranged by size:
5732 459 209 133
TatI w'GTAC_w
Cuts at: 636 1725 6048 636
Size: 1089 4323 1121
Fragments arranged by size:
4323 1121 1089
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6050 6082 6050
Size: 32 6501
Tth111I GACn'n_nGTC
Cuts at: 567 4330 567
Size: 3763 2770
XbaI T'CTAG_A
Cuts at: 571 1425 3564 571
Size: 854 2139 3540
Fragments arranged by size:
3540 2139 854
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 189 4028 189
Size: 3839 2694
XmaI C'CCGG_G
Cuts at: 117 3553 117
Size: 3436 3097
XmnI GAAnn'nnTTC
Cuts at: 344 1853 344
Size: 1509 5024
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PciI PflMI PmeI PpiI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI Bpu10I
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HpaI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI