Restriction map for
pF1KB9490
(Circular) (Six-base) MAPSORT of: pF1KB9490.seq Check: 5938 from: 1 to: 6677
pF1KB9490 6677 bp
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2465 3120 2465
Size: 655 6022
AccI GT'mk_AC
Cuts at: 3715 3715
Size: 6677
Acc65I G'GTAC_C
Cuts at: 236 3693 236
Size: 3457 3220
AclI AA'CG_TT
Cuts at: 6517 6517
Size: 6677
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1843 2567 3326 4502 4934 5287 5949 1843
Size: 724 759 1176 432 353 662 2571
Fragments arranged by size:
2571 1176 759 724 662 432 353
AflII C'TTAA_G
Cuts at: 807 807
Size: 6677
AflIII A'CryG_T
Cuts at: 136 948 4029 5829 6110 136
Size: 812 3081 1800 281 703
Fragments arranged by size:
3081 1800 812 703 281
AhdI GACnn_n'nnGTC
Cuts at: 2679 2679
Size: 6677
AleI CACnn'nnGTG
Cuts at: 3632 3632
Size: 6677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1730 1730
Size: 6677
AlwNI CAG_nnn'CTG
Cuts at: 2435 3982 5420 2435
Size: 1547 1438 3692
Fragments arranged by size:
3692 1547 1438
ApaLI G'TGCA_C
Cuts at: 5515 5515
Size: 6677
ApoI r'AATT_y
Cuts at: 155 607 659 677 716 846 987 3202 3295
Size: 452 52 18 39 130 141 2215 93
Cuts at: 3295 3566 3681 6030 6286 155
Size: 271 115 2349 256 546
Fragments arranged by size:
2349 2215 546 452 271 256 141 130
115 93 52 39 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 6677
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6677
AvaI C'yCGr_G
Cuts at: 1523 2101 3091 3697 1523
Size: 578 990 606 4503
Fragments arranged by size:
4503 990 606 578
BamHI G'GATC_C
Cuts at: 1085 3702 1085
Size: 2617 4060
BanI G'GyrC_C
Cuts at: 236 740 1415 3693 4107 4355 4390 236
Size: 504 675 2278 414 248 35 2523
Fragments arranged by size:
2523 2278 675 504 414 248 35
BanII G_rGCy'C
Cuts at: 2656 3284 3399 3455 3691 4721 2656
Size: 628 115 56 236 1030 4612
Fragments arranged by size:
4612 1030 628 236 115 56
BbeI G_GCGC'C
Cuts at: 4111 4359 4111
Size: 248 6429
BbsI GAAGACnn'nnnn_
Cuts at: 965 1748 1849 2573 2696 3332 3488 965
Size: 783 101 724 123 636 156 4154
Fragments arranged by size:
4154 783 724 636 156 123 101
BbvCI CC'TCA_GC
Cuts at: 3430 3430
Size: 6677
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1588 1622 1588
Size: 34 6643
BciVI GTATCCnnnnn_n'
Cuts at: 736 4575 5631 6677 736
Size: 3839 1056 1046 736
Fragments arranged by size:
3839 1056 1046 736
BclI T'GATC_A
Cuts at: 4197 4197
Size: 6677
BglII A'GATC_T
Cuts at: 330 1035 1689 2922 4192 6229 330
Size: 705 654 1233 1270 2037 778
Fragments arranged by size:
2037 1270 1233 778 705 654
BlpI GC'TnA_GC
Cuts at: 879 3789 879
Size: 2910 3767
Bme1580I G_kGCm'C
Cuts at: 4302 4395 5519 4302
Size: 93 1124 5460
Fragments arranged by size:
5460 1124 93
BmgBI CAC'GTC
Cuts at: 2676 2782 2676
Size: 106 6571
BmrI ACTGGGnnnn_n'
Cuts at: 1146 1844 3889 3970 4305 6449 1146
Size: 698 2045 81 335 2144 1374
Fragments arranged by size:
2144 2045 1374 698 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5044 5044
Size: 6677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1778 2015 2235 1778
Size: 237 220 6220
Fragments arranged by size:
6220 237 220
Bpu10I CC'TnA_GC
Cuts at: 736 3430 736
Size: 2694 3983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1362 3853 4732 5446 5744 472
Size: 890 2491 879 714 298 1405
Fragments arranged by size:
2491 1405 890 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 6381 6381
Size: 6677
BsaAI yAC'GTr
Cuts at: 4030 4030
Size: 6677
BsaBI GATnn'nnATC
Cuts at: 6 2207 2956 4191 6228 6
Size: 2201 749 1235 2037 455
Fragments arranged by size:
2201 2037 1235 749 455
BsaHI Gr'CG_yC
Cuts at: 4108 4356 5058 6554 4108
Size: 248 702 1496 4231
Fragments arranged by size:
4231 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3224 3866 4387 5065 5476 5623 5897 3224
Size: 642 521 678 411 147 274 4004
Fragments arranged by size:
4004 678 642 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 960 990 1570 1600 1754 1784 1988 2018 3638
Size: 30 580 30 154 30 204 30 1620
Cuts at: 3638 3668 960
Size: 30 3969
Fragments arranged by size:
3969 1620 580 204 154 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1684 2413 1684
Size: 729 5948
BseYI C'CCAG_C
Cuts at: 1465 2212 2589 2657 4102 4860 5525 5933 1465
Size: 747 377 68 1445 758 665 408 2209
Fragments arranged by size:
2209 1445 758 747 665 408 377 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 359 854 1559 2026 2698 2878 2994 359
Size: 495 705 467 672 180 116 4042
Fragments arranged by size:
4042 705 672 495 467 180 116
BsiEI CG_ry'CG
Cuts at: 104 4219 4265 5495 104
Size: 4115 46 1230 1286
Fragments arranged by size:
4115 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1524 2656 3284 3399 3691 4469 4659 5519 1524
Size: 1132 628 115 292 778 190 860 2682
Fragments arranged by size:
2682 1132 860 778 628 292 190 115
BsmI GAATG_Cn'
Cuts at: 488 3089 3239 488
Size: 2601 150 3926
Fragments arranged by size:
3926 2601 150
Bsp1286I G_dGCh'C
Cuts at: 1524 2656 3284 3399 3455 3691 4302 4395 4469
Size: 1132 628 115 56 236 611 93 74
Cuts at: 4469 4659 4721 5519 1524
Size: 190 62 798 2682
Fragments arranged by size:
2682 1132 798 628 611 236 190 115
93 74 62 56
BspEI T'CCGG_A
Cuts at: 3224 3224
Size: 6677
BspHI T'CATG_A
Cuts at: 143 821 1893 5109 143
Size: 678 1072 3216 1711
Fragments arranged by size:
3216 1711 1072 678
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2465 2850 3120 3727 3941 4243 4624 2465
Size: 385 270 607 214 302 381 4518
Fragments arranged by size:
4518 607 385 381 302 270 214
BsrBI CCG'CTC
Cuts at: 50 4969 5023 6507 6672 50
Size: 4919 54 1484 165 55
Fragments arranged by size:
4919 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4589 4589
Size: 6677
BsrFI r'CCGG_y
Cuts at: 4675 4856 4675
Size: 181 6496
BssSI C'ACGA_G
Cuts at: 4948 5656 4948
Size: 708 5969
BstAPI GCAn_nnn'nTGC
Cuts at: 1692 4054 1692
Size: 2362 4315
BstBI TT'CG_AA
Cuts at: 2052 5038 2052
Size: 2986 3691
BstEII G'GTnAC_C
Cuts at: 1042 2133 1042
Size: 1091 5586
BstXI CCAn_nnnn'nTGG
Cuts at: 1361 1759 2241 1361
Size: 398 482 5797
Fragments arranged by size:
5797 482 398
BstYI r'GATC_y
Cuts at: 1 330 1035 1085 1218 1364 1689 2542 2922
Size: 329 705 50 133 146 325 853 380
Cuts at: 2922 3702 4169 4192 4527 4773 5079 5091 5177
Size: 780 467 23 335 246 306 12 86
Cuts at: 5177 6229 1
Size: 1052 449
Fragments arranged by size:
1052 853 780 705 467 449 380 335
329 325 306 246 146 133 86 50
23 12
BtgI C'CryG_G
Cuts at: 107 2393 107
Size: 2286 4391
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4627 4808 4971 4627
Size: 181 163 6333
Fragments arranged by size:
6333 181 163
BtsI GCAGTG_nn'
Cuts at: 302 2264 2359 3920 302
Size: 1962 95 1561 3059
Fragments arranged by size:
3059 1962 1561 95
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 512 547 512
Size: 35 6642
DraI TTT'AAA
Cuts at: 786 2993 3676 786
Size: 2207 683 3787
Fragments arranged by size:
3787 2207 683
DrdI GACnn_nn'nnGTC
Cuts at: 1853 4383 5727 1853
Size: 2530 1344 2803
Fragments arranged by size:
2803 2530 1344
EaeI y'GGCC_r
Cuts at: 1443 1772 4262 4436 4827 4854 6199 1443
Size: 329 2490 174 391 27 1345 1921
Fragments arranged by size:
2490 1921 1345 391 329 174 27
EagI C'GGCC_G
Cuts at: 4262 4262
Size: 6677
EarI CTCTTCn'nnn_
Cuts at: 456 2319 4700 4910 456
Size: 1863 2381 210 2223
Fragments arranged by size:
2381 2223 1863 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2535 4885 5627 5773 6487 2535
Size: 2350 742 146 714 2725
Fragments arranged by size:
2725 2350 742 714 146
EcoICRI GAG'CTC
Cuts at: 2654 3282 3397 3689 2654
Size: 628 115 292 5642
Fragments arranged by size:
5642 628 292 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1778 1843 2015 2235 2567 3326 4502 4934 5287
Size: 65 172 220 332 759 1176 432 353
Cuts at: 5287 5949 1778
Size: 662 2506
Fragments arranged by size:
2506 1176 759 662 432 353 332 220
172 65
EcoO109I rG'GnC_Cy
Cuts at: 838 1846 2447 2705 3816 838
Size: 1008 601 258 1111 3699
Fragments arranged by size:
3699 1111 1008 601 258
EcoRI G'AATT_C
Cuts at: 3202 3295 3681 3202
Size: 93 386 6198
Fragments arranged by size:
6198 386 93
EcoRV GAT'ATC
Cuts at: 255 1401 255
Size: 1146 5531
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 811 1054 4696 811
Size: 243 3642 2792
Fragments arranged by size:
3642 2792 243
FspI TGC'GCA
Cuts at: 3238 4458 3238
Size: 1220 5457
FspAI rTGC'GCAy
Cuts at: 3238 3238
Size: 6677
HaeII r_GCGC'y
Cuts at: 4111 4359 5589 6360 4111
Size: 248 1230 771 4428
Fragments arranged by size:
4428 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 291 323 926 958 2665 2697 4519 4549 4551
Size: 32 603 32 1707 32 1822 30 2
Cuts at: 4551 4581 291
Size: 30 2387
Fragments arranged by size:
2387 1822 1707 603 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3716 3716
Size: 6677
HindIII A'AGCT_T
Cuts at: 506 506
Size: 6677
KasI G'GCGC_C
Cuts at: 4107 4355 4107
Size: 248 6429
KpnI G_GTAC'C
Cuts at: 240 3697 240
Size: 3457 3220
MfeI C'AATT_G
Cuts at: 1332 1359 2907 3011 1332
Size: 27 1548 104 4998
Fragments arranged by size:
4998 1548 104 27
MluI A'CGCG_T
Cuts at: 6110 6110
Size: 6677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 946 969 1121 1613 2871 5436 5620 946
Size: 23 152 492 1258 2565 184 2003
Fragments arranged by size:
2565 2003 1258 492 184 152 23
MscI TGG'CCA
Cuts at: 1445 4438 1445
Size: 2993 3684
MslI CAynn'nnrTG
Cuts at: 262 1655 3544 3632 4666 4793 262
Size: 1393 1889 88 1034 127 2146
Fragments arranged by size:
2146 1889 1393 1034 127 88
MspA1I CmG'CkG
Cuts at: 1720 2114 2216 2306 3248 3528 3788 4102 4462
Size: 394 102 90 942 280 260 314 360
Cuts at: 4462 5246 5491 1720
Size: 784 245 2906
Fragments arranged by size:
2906 942 784 394 360 314 280 260
245 102 90
NaeI GCC'GGC
Cuts at: 4858 4858
Size: 6677
NarI GG'CG_CC
Cuts at: 4108 4356 4108
Size: 248 6429
NcoI C'CATG_G
Cuts at: 107 2393 107
Size: 2286 4391
NgoMIV G'CCGG_C
Cuts at: 4856 4856
Size: 6677
NspI r_CATG'y
Cuts at: 140 363 521 952 3730 4230 5833 140
Size: 223 158 431 2778 500 1603 984
Fragments arranged by size:
2778 1603 984 500 431 223 158
PasI CC'CwG_GG
Cuts at: 3353 3353
Size: 6677
PciI A'CATG_T
Cuts at: 136 948 5829 136
Size: 812 4881 984
Fragments arranged by size:
4881 984 812
PflMI CCAn_nnn'nTGG
Cuts at: 2076 2076
Size: 6677
PfoI T'CCnGG_A
Cuts at: 277 492 277
Size: 215 6462
PmeI GTTT'AAAC
Cuts at: 3676 3676
Size: 6677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5123 5155 5123
Size: 32 6645
PpuMI rG'GwC_Cy
Cuts at: 838 1846 838
Size: 1008 5669
PshAI GACnn'nnGTC
Cuts at: 3528 3528
Size: 6677
PspXI vC'TCGA_Gb
Cuts at: 1523 2101 1523
Size: 578 6099
PstI C_TGCA'G
Cuts at: 251 1697 2221 3450 3537 3724 4409 251
Size: 1446 524 1229 87 187 685 2519
Fragments arranged by size:
2519 1446 1229 685 524 187 87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6677
PvuII CAG'CTG
Cuts at: 1720 2114 2216 3248 3528 4102 4462 1720
Size: 394 102 1032 280 574 360 3935
Fragments arranged by size:
3935 1032 574 394 360 280 102
RsrII CG'GwC_CG
Cuts at: 4872 4872
Size: 6677
SacI G_AGCT'C
Cuts at: 2656 3284 3399 3691 2656
Size: 628 115 292 5642
Fragments arranged by size:
5642 628 292 115
SalI G'TCGA_C
Cuts at: 3714 3714
Size: 6677
SapI GCTCTTCn'nnn_
Cuts at: 2319 4700 4910 2319
Size: 2381 210 4086
Fragments arranged by size:
4086 2381 210
SbfI CC_TGCA'GG
Cuts at: 3724 3724
Size: 6677
ScaI AGT'ACT
Cuts at: 218 6194 218
Size: 5976 701
SexAI A'CCwGG_T
Cuts at: 2968 2968
Size: 6677
SfcI C'TryA_G
Cuts at: 33 247 665 1195 1693 2217 2330 2630 3260
Size: 214 418 530 498 524 113 300 630
Cuts at: 3260 3446 3533 3720 4405 5373 5564 33
Size: 186 87 187 685 968 191 1146
Fragments arranged by size:
1146 968 685 630 530 524 498 418
300 214 191 187 186 113 87
SfoI GGC'GCC
Cuts at: 4109 4357 4109
Size: 248 6429
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6677
SmaI CCC'GGG
Cuts at: 3699 3699
Size: 6677
SmlI C'TyrA_G
Cuts at: 451 807 1377 1523 2101 3832 4747 5461 5723
Size: 356 570 146 578 1731 915 714 262
Cuts at: 5723 451
Size: 1405
Fragments arranged by size:
1731 1405 915 714 578 570 356 262
146
SpeI A'CTAG_T
Cuts at: 67 2821 67
Size: 2754 3923
SphI G_CATG'C
Cuts at: 521 3730 4230 521
Size: 3209 500 2968
Fragments arranged by size:
3209 2968 500
StyI C'CwwG_G
Cuts at: 107 930 1511 2393 3424 3811 4165 107
Size: 823 581 882 1031 387 354 2619
Fragments arranged by size:
2619 1031 882 823 581 387 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 523 1524 4260 4393 4852 5061 523
Size: 1001 2736 133 459 209 2139
Fragments arranged by size:
2736 2139 1001 459 209 133
TatI w'GTAC_w
Cuts at: 216 2739 2824 6192 216
Size: 2523 85 3368 701
Fragments arranged by size:
3368 2523 701 85
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 244 276 960 992 1754 1786 1988 2020 6194
Size: 32 684 32 762 32 202 32 4174
Cuts at: 6194 6226 244
Size: 32 695
Fragments arranged by size:
4174 762 695 684 202 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1747 4474 1747
Size: 2727 3950
XbaI T'CTAG_A
Cuts at: 1926 3708 1926
Size: 1782 4895
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4172 4172
Size: 6677
XhoI C'TCGA_G
Cuts at: 1523 2101 1523
Size: 578 6099
XmaI C'CCGG_G
Cuts at: 3697 3697
Size: 6677
XmnI GAAnn'nnTTC
Cuts at: 914 1163 914
Size: 249 6428
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AloI ApaI AscI AvrII BaeI
BfrBI BglI BmtI BsiWI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI DraIII EcoNI FseI HpaI NdeI NheI
NotI NruI NsiI PacI PmlI PsiI PspOMI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI ZraI