Restriction map for
pF1KB9488
(Circular) (Six-base) MAPSORT of: pF1KB9488.seq Check: 9814 from: 1 to: 6512
pF1KB9488 6512 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 303 3474 3550 303
Size: 3171 76 3265
Fragments arranged by size:
3265 3171 76
Acc65I G'GTAC_C
Cuts at: 3528 3528
Size: 6512
AclI AA'CG_TT
Cuts at: 6352 6352
Size: 6512
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 789 859 1390 1627 1960 2147 2153 4337
Size: 235 70 531 237 333 187 6 2184
Cuts at: 4337 4769 5122 5784 554
Size: 432 353 662 1282
Fragments arranged by size:
2184 1282 662 531 432 353 333 237
235 187 70 6
AflII C'TTAA_G
Cuts at: 2859 2859
Size: 6512
AflIII A'CryG_T
Cuts at: 3864 5664 5945 3864
Size: 1800 281 4431
Fragments arranged by size:
4431 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1698 1698
Size: 6512
AleI CACnn'nnGTG
Cuts at: 466 466
Size: 6512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 606 606
Size: 6512
AlwNI CAG_nnn'CTG
Cuts at: 1797 3375 3817 5255 1797
Size: 1578 442 1438 3054
Fragments arranged by size:
3054 1578 1438 442
ApaI G_GGCC'C
Cuts at: 2880 2880
Size: 6512
ApaLI G'TGCA_C
Cuts at: 5350 5350
Size: 6512
ApoI r'AATT_y
Cuts at: 742 1352 1811 2785 3248 3516 5865 6121 742
Size: 610 459 974 463 268 2349 256 1133
Fragments arranged by size:
2349 1133 974 610 463 459 268 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6512
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6512
AvaI C'yCGr_G
Cuts at: 2938 3532 2938
Size: 594 5918
BamHI G'GATC_C
Cuts at: 3537 3537
Size: 6512
BanI G'GyrC_C
Cuts at: 384 3528 3942 4190 4225 384
Size: 3144 414 248 35 2671
Fragments arranged by size:
3144 2671 414 248 35
BanII G_rGCy'C
Cuts at: 115 885 2880 2903 3263 3526 4556 115
Size: 770 1995 23 360 263 1030 2071
Fragments arranged by size:
2071 1995 1030 770 360 263 23
BbeI G_GCGC'C
Cuts at: 3946 4194 3946
Size: 248 6264
BbsI GAAGACnn'nnnn_
Cuts at: 2524 2992 2524
Size: 468 6044
BbvCI CC'TCA_GC
Cuts at: 3075 3075
Size: 6512
BciVI GTATCCnnnnn_n'
Cuts at: 302 1244 3319 3358 4410 5466 6512 302
Size: 942 2075 39 1052 1056 1046 302
Fragments arranged by size:
2075 1056 1052 1046 942 302 39
BclI T'GATC_A
Cuts at: 2345 4032 2345
Size: 1687 4825
BfrBI ATG'CAT
Cuts at: 598 1057 598
Size: 459 6053
BglI GCCn_nnn'nGGC
Cuts at: 2260 2260
Size: 6512
BglII A'GATC_T
Cuts at: 938 3162 4027 6064 938
Size: 2224 865 2037 1386
Fragments arranged by size:
2224 2037 1386 865
BlpI GC'TnA_GC
Cuts at: 3624 3624
Size: 6512
Bme1580I G_kGCm'C
Cuts at: 1453 2880 4137 4230 5354 1453
Size: 1427 1257 93 1124 2611
Fragments arranged by size:
2611 1427 1257 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 429 2322 3257 3724 3805 4140 6284 429
Size: 1893 935 467 81 335 2144 657
Fragments arranged by size:
2144 1893 935 657 467 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4879 4879
Size: 6512
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2887 2993 2887
Size: 106 6406
Bpu10I CC'TnA_GC
Cuts at: 2288 3075 3430 2288
Size: 787 355 5370
Fragments arranged by size:
5370 787 355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2051 2930 3688 4567 5281 5579 2051
Size: 879 758 879 714 298 2984
Fragments arranged by size:
2984 879 879 758 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 3292 6216 3292
Size: 2924 3588
BsaAI yAC'GTr
Cuts at: 2526 3126 3307 3865 2526
Size: 600 181 558 5173
Fragments arranged by size:
5173 600 558 181
BsaBI GATnn'nnATC
Cuts at: 6 1934 4026 6063 6
Size: 1928 2092 2037 455
Fragments arranged by size:
2092 2037 1928 455
BsaHI Gr'CG_yC
Cuts at: 221 3943 4191 4893 6389 221
Size: 3722 248 702 1496 344
Fragments arranged by size:
3722 1496 702 344 248
BsaWI w'CCGG_w
Cuts at: 3701 4222 4900 5311 5458 5732 3701
Size: 521 678 411 147 274 4481
Fragments arranged by size:
4481 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 545 575 3296 3326 545
Size: 30 2721 30 3731
Fragments arranged by size:
3731 2721 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2237 2240 2243 2237
Size: 3 3 6506
Fragments arranged by size:
6506 3 3
BseYI C'CCAG_C
Cuts at: 3937 4695 5360 5768 3937
Size: 758 665 408 4681
Fragments arranged by size:
4681 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1052 1891 2381 1052
Size: 839 490 5183
Fragments arranged by size:
5183 839 490
BsiEI CG_ry'CG
Cuts at: 104 4054 4100 5330 104
Size: 3950 46 1230 1286
Fragments arranged by size:
3950 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 275 1615 2810 2903 3526 4304 4494 5354 275
Size: 1340 1195 93 623 778 190 860 1433
Fragments arranged by size:
1433 1340 1195 860 778 623 190 93
BsmI GAATG_Cn'
Cuts at: 2995 3162 2995
Size: 167 6345
BsmBI CGTCTCn'nnnn_
Cuts at: 2182 2182
Size: 6512
Bsp1286I G_dGCh'C
Cuts at: 115 275 885 1453 1615 2810 2880 2903 3263
Size: 160 610 568 162 1195 70 23 360
Cuts at: 3263 3526 4137 4230 4304 4494 4556 5354 115
Size: 263 611 93 74 190 62 798 1273
Fragments arranged by size:
1273 1195 798 611 610 568 360 263
190 162 160 93 74 70 62 23
BspHI T'CATG_A
Cuts at: 4944 4944
Size: 6512
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3562 3776 4078 4459 3562
Size: 214 302 381 5615
Fragments arranged by size:
5615 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1185 4804 4858 6342 6507 50
Size: 1135 3619 54 1484 165 55
Fragments arranged by size:
3619 1484 1135 165 55 54
BsrDI GCAATG_nn'
Cuts at: 563 600 1425 2190 3086 4424 563
Size: 37 825 765 896 1338 2651
Fragments arranged by size:
2651 1338 896 825 765 37
BsrFI r'CCGG_y
Cuts at: 149 4510 4691 149
Size: 4361 181 1970
Fragments arranged by size:
4361 1970 181
BsrGI T'GTAC_A
Cuts at: 867 2373 867
Size: 1506 5006
BssSI C'ACGA_G
Cuts at: 4783 5491 4783
Size: 708 5804
BstAPI GCAn_nnn'nTGC
Cuts at: 1174 3333 3889 1174
Size: 2159 556 3797
Fragments arranged by size:
3797 2159 556
BstBI TT'CG_AA
Cuts at: 4873 4873
Size: 6512
BstEII G'GTnAC_C
Cuts at: 280 280
Size: 6512
BstXI CCAn_nnnn'nTGG
Cuts at: 468 1084 468
Size: 616 5896
BstYI r'GATC_y
Cuts at: 1 344 938 2169 3162 3537 4004 4027 4362
Size: 343 594 1231 993 375 467 23 335
Cuts at: 4362 4608 4914 4926 5012 6064 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1231 1052 993 594 467 449 375 343
335 306 246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4462 4643 4806 4462
Size: 181 163 6168
Fragments arranged by size:
6168 181 163
BtsI GCAGTG_nn'
Cuts at: 521 1879 3755 521
Size: 1358 1876 3278
Fragments arranged by size:
3278 1876 1358
DraI TTT'AAA
Cuts at: 2210 3511 2210
Size: 1301 5211
DraIII CAC_nnn'GTG
Cuts at: 122 651 122
Size: 529 5983
DrdI GACnn_nn'nnGTC
Cuts at: 2436 4218 5562 2436
Size: 1782 1344 3386
Fragments arranged by size:
3386 1782 1344
EaeI y'GGCC_r
Cuts at: 4097 4271 4662 4689 6034 4097
Size: 174 391 27 1345 4575
Fragments arranged by size:
4575 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4097 4097
Size: 6512
EarI CTCTTCn'nnn_
Cuts at: 717 1415 2537 4535 4745 717
Size: 698 1122 1998 210 2484
Fragments arranged by size:
2484 1998 1122 698 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4720 5462 5608 6322 4720
Size: 742 146 714 4910
Fragments arranged by size:
4910 742 714 146
EcoICRI GAG'CTC
Cuts at: 2901 3524 2901
Size: 623 5889
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 789 859 1390 1627 1960 2147 2153 2887
Size: 235 70 531 237 333 187 6 734
Cuts at: 2887 2993 4337 4769 5122 5784 554
Size: 106 1344 432 353 662 1282
Fragments arranged by size:
1344 1282 734 662 531 432 353 333
237 235 187 106 70 6
EcoO109I rG'GnC_Cy
Cuts at: 2877 3071 3651 2877
Size: 194 580 5738
Fragments arranged by size:
5738 580 194
EcoRI G'AATT_C
Cuts at: 1352 3516 1352
Size: 2164 4348
EcoRV GAT'ATC
Cuts at: 1770 1770
Size: 6512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3246 4531 3246
Size: 1285 5227
FspI TGC'GCA
Cuts at: 4293 4293
Size: 6512
HaeII r_GCGC'y
Cuts at: 3946 4194 5424 6195 3946
Size: 248 1230 771 4263
Fragments arranged by size:
4263 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 231 263 545 577 1684 1716 3154 3186 3293
Size: 32 282 32 1107 32 1438 32 107
Cuts at: 3293 3325 3475 3507 4354 4384 4386 4416 231
Size: 32 150 32 847 30 2 30 2327
Fragments arranged by size:
2327 1438 1107 847 282 150 107 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1121 3475 3551 1121
Size: 2354 76 4082
Fragments arranged by size:
4082 2354 76
HindIII A'AGCT_T
Cuts at: 1220 1520 1220
Size: 300 6212
HpaI GTT'AAC
Cuts at: 1121 1121
Size: 6512
KasI G'GCGC_C
Cuts at: 3942 4190 3942
Size: 248 6264
KpnI G_GTAC'C
Cuts at: 3532 3532
Size: 6512
MfeI C'AATT_G
Cuts at: 980 2750 980
Size: 1770 4742
MluI A'CGCG_T
Cuts at: 5945 5945
Size: 6512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2571 5271 5455 2571
Size: 2700 184 3628
Fragments arranged by size:
3628 2700 184
MscI TGG'CCA
Cuts at: 4273 4273
Size: 6512
MslI CAynn'nnrTG
Cuts at: 324 466 1910 2735 3155 4501 4628 324
Size: 142 1444 825 420 1346 127 2208
Fragments arranged by size:
2208 1444 1346 825 420 142 127
MspA1I CmG'CkG
Cuts at: 1368 2479 2616 3375 3426 3623 3937 4297 5081
Size: 1111 137 759 51 197 314 360 784
Cuts at: 5081 5326 1368
Size: 245 2554
Fragments arranged by size:
2554 1111 784 759 360 314 245 197
137 51
NaeI GCC'GGC
Cuts at: 151 4693 151
Size: 4542 1970
NarI GG'CG_CC
Cuts at: 3943 4191 3943
Size: 248 6264
NdeI CA'TA_TG
Cuts at: 321 321
Size: 6512
NgoMIV G'CCGG_C
Cuts at: 149 4691 149
Size: 4542 1970
NsiI A_TGCA'T
Cuts at: 600 1059 600
Size: 459 6053
NspI r_CATG'y
Cuts at: 602 694 3565 4065 5668 602
Size: 92 2871 500 1603 1446
Fragments arranged by size:
2871 1603 1446 500 92
PasI CC'CwG_GG
Cuts at: 2873 2873
Size: 6512
PciI A'CATG_T
Cuts at: 5664 5664
Size: 6512
PflMI CCAn_nnn'nTGG
Cuts at: 122 651 3016 122
Size: 529 2365 3618
Fragments arranged by size:
3618 2365 529
PmeI GTTT'AAAC
Cuts at: 3511 3511
Size: 6512
PmlI CAC'GTG
Cuts at: 2526 3126 2526
Size: 600 5912
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4958 4990 4958
Size: 32 6480
PpuMI rG'GwC_Cy
Cuts at: 3071 3071
Size: 6512
PsiI TTA'TAA
Cuts at: 194 664 928 2326 194
Size: 470 264 1398 4380
Fragments arranged by size:
4380 1398 470 264
PspOMI G'GGCC_C
Cuts at: 2876 2876
Size: 6512
PspXI vC'TCGA_Gb
Cuts at: 2938 2938
Size: 6512
PstI C_TGCA'G
Cuts at: 585 3507 3559 4244 585
Size: 2922 52 685 2853
Fragments arranged by size:
2922 2853 685 52
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6512
PvuII CAG'CTG
Cuts at: 1368 2616 3375 3426 3937 4297 1368
Size: 1248 759 51 511 360 3583
Fragments arranged by size:
3583 1248 759 511 360 51
RsrII CG'GwC_CG
Cuts at: 4707 4707
Size: 6512
SacI G_AGCT'C
Cuts at: 2903 3526 2903
Size: 623 5889
SalI G'TCGA_C
Cuts at: 3473 3549 3473
Size: 76 6436
SapI GCTCTTCn'nnn_
Cuts at: 4535 4745 4535
Size: 210 6302
SbfI CC_TGCA'GG
Cuts at: 3559 3559
Size: 6512
ScaI AGT'ACT
Cuts at: 6029 6029
Size: 6512
SfcI C'TryA_G
Cuts at: 33 581 942 1598 3214 3503 3555 4240 5208
Size: 548 361 656 1616 289 52 685 968
Cuts at: 5208 5399 33
Size: 191 1146
Fragments arranged by size:
1616 1146 968 685 656 548 361 289
191 52
SfoI GGC'GCC
Cuts at: 3944 4192 3944
Size: 248 6264
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6512
SmaI CCC'GGG
Cuts at: 3534 3534
Size: 6512
SmlI C'TyrA_G
Cuts at: 2066 2859 2938 2945 3667 4582 5296 5558 2066
Size: 793 79 7 722 915 714 262 3020
Fragments arranged by size:
3020 915 793 722 714 262 79 7
SnaBI TAC'GTA
Cuts at: 3307 3307
Size: 6512
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6512
SphI G_CATG'C
Cuts at: 3565 4065 3565
Size: 500 6012
SspI AAT'ATT
Cuts at: 897 1495 1833 3024 897
Size: 598 338 1191 4385
Fragments arranged by size:
4385 1191 598 338
StuI AGG'CCT
Cuts at: 1081 1081
Size: 6512
StyI C'CwwG_G
Cuts at: 1665 3646 4000 1665
Size: 1981 354 4177
Fragments arranged by size:
4177 1981 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1823 2258 3241 4095 4228 4687 4896 1823
Size: 435 983 854 133 459 209 3439
Fragments arranged by size:
3439 983 854 459 435 209 133
TatI w'GTAC_w
Cuts at: 627 867 1848 2373 6027 627
Size: 240 981 525 3654 1112
Fragments arranged by size:
3654 1112 981 525 240
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2186 2218 2568 2600 6029 6061 2186
Size: 32 350 32 3429 32 2637
Fragments arranged by size:
3429 2637 350 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4309 4309
Size: 6512
XbaI T'CTAG_A
Cuts at: 3543 3543
Size: 6512
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2925 4007 2925
Size: 1082 5430
XhoI C'TCGA_G
Cuts at: 2938 2938
Size: 6512
XmaI C'CCGG_G
Cuts at: 3532 3532
Size: 6512
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NsiI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AloI AscI AvrII BaeI
BcgI BmgBI BmtI BsiWI BspEI BssHII BstZ17I Bsu36I
BtgI ClaI CspCI EcoNI FseI FspAI NcoI NheI
NotI NruI PacI PfoI PshAI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SwaI XmnI ZraI