Restriction map for
pF1KB9487
(Circular) (Six-base) MAPSORT of: pF1KB9487.seq Check: 8810 from: 1 to: 6674
pF1KB9487 6674 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 901 1622 3626 3712 901
Size: 721 2004 86 3863
Fragments arranged by size:
3863 2004 721 86
Acc65I G'GTAC_C
Cuts at: 1677 3690 1677
Size: 2013 4661
AclI AA'CG_TT
Cuts at: 738 6514 738
Size: 5776 898
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 373 418 1782 2827 4499 4931 5284 5946
Size: 231 45 1364 1045 1672 432 353 662
Cuts at: 5946 142
Size: 870
Fragments arranged by size:
1672 1364 1045 870 662 432 353 231
45
AflII C'TTAA_G
Cuts at: 1783 1783
Size: 6674
AflIII A'CryG_T
Cuts at: 3071 4026 5826 6107 3071
Size: 955 1800 281 3638
Fragments arranged by size:
3638 1800 955 281
AleI CACnn'nnGTG
Cuts at: 3072 3470 3072
Size: 398 6276
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2779 2811 2779
Size: 32 6642
AlwNI CAG_nnn'CTG
Cuts at: 267 709 1503 3979 5417 267
Size: 442 794 2476 1438 1524
Fragments arranged by size:
2476 1524 1438 794 442
ApaI G_GGCC'C
Cuts at: 3062 3062
Size: 6674
ApaLI G'TGCA_C
Cuts at: 210 5512 210
Size: 5302 1372
ApoI r'AATT_y
Cuts at: 370 1044 2852 3678 6027 6283 370
Size: 674 1808 826 2349 256 761
Fragments arranged by size:
2349 1808 826 761 674 256
AseI AT'TA_AT
Cuts at: 20 2996 20
Size: 2976 3698
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6674
AvaI C'yCGr_G
Cuts at: 1386 2137 2735 2774 3694 1386
Size: 751 598 39 920 4366
Fragments arranged by size:
4366 920 751 598 39
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1332 1365 1332
Size: 33 6641
BamHI G'GATC_C
Cuts at: 1472 3699 1472
Size: 2227 4447
BanI G'GyrC_C
Cuts at: 1586 1677 2268 3433 3446 3690 4104 4352 4387
Size: 91 591 1165 13 244 414 248 35
Cuts at: 4387 1586
Size: 3873
Fragments arranged by size:
3873 1165 591 414 248 244 91 35
13
BanII G_rGCy'C
Cuts at: 920 1988 2397 2726 3062 3688 4718 920
Size: 1068 409 329 336 626 1030 2876
Fragments arranged by size:
2876 1068 1030 626 409 336 329
BbeI G_GCGC'C
Cuts at: 4108 4356 4108
Size: 248 6426
BbsI GAAGACnn'nnnn_
Cuts at: 852 1933 2200 2624 852
Size: 1081 267 424 4902
Fragments arranged by size:
4902 1081 424 267
BbvCI CC'TCA_GC
Cuts at: 2449 3565 2449
Size: 1116 5558
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 186 220 1395 1429 90
Size: 34 62 34 1175 34 5335
Fragments arranged by size:
5335 1175 62 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1344 1360 2983 4572 5628 6674 1344
Size: 16 1623 1589 1056 1046 1344
Fragments arranged by size:
1623 1589 1344 1056 1046 16
BclI T'GATC_A
Cuts at: 2645 4194 2645
Size: 1549 5125
BglII A'GATC_T
Cuts at: 4189 6226 4189
Size: 2037 4637
BlpI GC'TnA_GC
Cuts at: 3201 3786 3201
Size: 585 6089
Bme1580I G_kGCm'C
Cuts at: 214 1418 2273 2313 3062 3557 4299 4392 5516
Size: 1204 855 40 749 495 742 93 1124
Cuts at: 5516 214
Size: 1372
Fragments arranged by size:
1372 1204 1124 855 749 742 495 93
40
BmgBI CAC'GTC
Cuts at: 1131 1131
Size: 6674
BmrI ACTGGGnnnn_n'
Cuts at: 3886 3967 4302 6446 3886
Size: 81 335 2144 4114
Fragments arranged by size:
4114 2144 335 81
BmtI G_CTAG'C
Cuts at: 532 532
Size: 6674
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1167 1517 1962 5041 1167
Size: 350 445 3079 2800
Fragments arranged by size:
3079 2800 445 350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 289 361 664 1098 1954 2056 2243 2701
Size: 21 72 303 434 856 102 187 458
Cuts at: 2701 3642 268
Size: 941 3300
Fragments arranged by size:
3300 941 856 458 434 303 187 102
72 21
Bpu10I CC'TnA_GC
Cuts at: 154 2449 3565 154
Size: 2295 1116 3263
Fragments arranged by size:
3263 2295 1116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 566 2065 3850 4729 5443 5741 566
Size: 1499 1785 879 714 298 1499
Fragments arranged by size:
1785 1499 1499 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2583 6378 2583
Size: 3795 2879
BsaAI yAC'GTr
Cuts at: 3074 4027 3074
Size: 953 5721
BsaBI GATnn'nnATC
Cuts at: 6 721 2644 3375 4188 6225 6
Size: 715 1923 731 813 2037 455
Fragments arranged by size:
2037 1923 813 731 715 455
BsaHI Gr'CG_yC
Cuts at: 1065 4105 4353 5055 6551 1065
Size: 3040 248 702 1496 1188
Fragments arranged by size:
3040 1496 1188 702 248
BsaWI w'CCGG_w
Cuts at: 1569 1950 3863 4384 5062 5473 5620 5894 1569
Size: 381 1913 521 678 411 147 274 2349
Fragments arranged by size:
2349 1913 678 521 411 381 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3101 3131 3382 3412 3101
Size: 30 251 30 6363
Fragments arranged by size:
6363 251 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 661 2155 2254 2257 2260 2263 2266 2959 3154
Size: 1494 99 3 3 3 3 693 195
Cuts at: 3154 3157 3403 3562 661
Size: 3 246 159 3773
Fragments arranged by size:
3773 1494 693 246 195 159 99 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 2168 2312 3475 4099 4857 5522 5930 2168
Size: 144 1163 624 758 665 408 2912
Fragments arranged by size:
2912 1163 758 665 624 408 144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1014 1933 1014
Size: 919 5755
BsiEI CG_ry'CG
Cuts at: 104 4216 4262 5492 104
Size: 4112 46 1230 1286
Fragments arranged by size:
4112 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 214 920 1148 1723 3500 3688 4466 4656 5516
Size: 706 228 575 1777 188 778 190 860
Cuts at: 5516 214
Size: 1372
Fragments arranged by size:
1777 1372 860 778 706 575 228 190
188
BsmI GAATG_Cn'
Cuts at: 247 247
Size: 6674
BsmBI CGTCTCn'nnnn_
Cuts at: 274 274
Size: 6674
Bsp1286I G_dGCh'C
Cuts at: 214 920 1148 1418 1723 1988 2273 2313 2397
Size: 706 228 270 305 265 285 40 84
Cuts at: 2397 2726 3062 3500 3557 3688 4299 4392 4466
Size: 329 336 438 57 131 611 93 74
Cuts at: 4466 4656 4718 5516 214
Size: 190 62 798 1372
Fragments arranged by size:
1372 798 706 611 438 336 329 305
285 270 265 228 190 131 93 84
74 62 57 40
BspHI T'CATG_A
Cuts at: 3593 5106 3593
Size: 1513 5161
BspMI ACCTGCnnnn'nnnn_
Cuts at: 473 1839 3724 3938 4240 4621 473
Size: 1366 1885 214 302 381 2526
Fragments arranged by size:
2526 1885 1366 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2562 4966 5020 6504 6669 50
Size: 2512 2404 54 1484 165 55
Fragments arranged by size:
2512 2404 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 204 2394 4586 204
Size: 2190 2192 2292
Fragments arranged by size:
2292 2192 2190
BsrFI r'CCGG_y
Cuts at: 532 1082 1394 2496 3443 3490 4672 4853 532
Size: 550 312 1102 947 47 1182 181 2353
Fragments arranged by size:
2353 1182 1102 947 550 312 181 47
BsrGI T'GTAC_A
Cuts at: 505 719 795 1559 505
Size: 214 76 764 5620
Fragments arranged by size:
5620 764 214 76
BssSI C'ACGA_G
Cuts at: 2158 4945 5653 2158
Size: 2787 708 3179
Fragments arranged by size:
3179 2787 708
BstAPI GCAn_nnn'nTGC
Cuts at: 164 4051 164
Size: 3887 2787
BstBI TT'CG_AA
Cuts at: 3240 5035 3240
Size: 1795 4879
BstEII G'GTnAC_C
Cuts at: 1899 3225 1899
Size: 1326 5348
BstXI CCAn_nnnn'nTGG
Cuts at: 912 912
Size: 6674
BstYI r'GATC_y
Cuts at: 1 1472 1551 2276 3392 3699 4166 4189 4524
Size: 1471 79 725 1116 307 467 23 335
Cuts at: 4524 4770 5076 5088 5174 6226 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1471 1116 1052 725 467 449 335 307
306 246 86 79 23 12
Bsu36I CC'TnA_GG
Cuts at: 3574 3574
Size: 6674
BtgI C'CryG_G
Cuts at: 1819 2007 2099 2429 2783 3125 3266 1819
Size: 188 92 330 354 342 141 5227
Fragments arranged by size:
5227 354 342 330 188 141 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4624 4805 4968 4624
Size: 181 163 6330
Fragments arranged by size:
6330 181 163
BtsI GCAGTG_nn'
Cuts at: 3131 3917 3131
Size: 786 5888
DraI TTT'AAA
Cuts at: 1244 3673 1244
Size: 2429 4245
DraIII CAC_nnn'GTG
Cuts at: 1716 1716
Size: 6674
DrdI GACnn_nn'nnGTC
Cuts at: 2082 4380 5724 2082
Size: 2298 1344 3032
Fragments arranged by size:
3032 2298 1344
EaeI y'GGCC_r
Cuts at: 1733 1822 1853 1994 2432 3337 4259 4433 4824
Size: 89 31 141 438 905 922 174 391
Cuts at: 4824 4851 6196 1733
Size: 27 1345 2211
Fragments arranged by size:
2211 1345 922 905 438 391 174 141
89 31 27
EagI C'GGCC_G
Cuts at: 4259 4259
Size: 6674
EarI CTCTTCn'nnn_
Cuts at: 626 926 1459 1801 2371 2686 2747 2816 4697
Size: 300 533 342 570 315 61 69 1881
Cuts at: 4697 4907 626
Size: 210 2393
Fragments arranged by size:
2393 1881 570 533 342 315 300 210
69 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 118 4882 5624 5770 6484 118
Size: 4764 742 146 714 308
Fragments arranged by size:
4764 742 714 308 146
EcoICRI GAG'CTC
Cuts at: 918 3686 918
Size: 2768 3906
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 268 289 361 373 418 664 1098 1782
Size: 126 21 72 12 45 246 434 684
Cuts at: 1782 1954 2056 2243 2701 2827 3642 4499 4931
Size: 172 102 187 458 126 815 857 432
Cuts at: 4931 5284 5946 142
Size: 353 662 870
Fragments arranged by size:
870 857 815 684 662 458 434 432
353 246 187 172 126 126 102 72
45 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 631 1422 631
Size: 791 5883
EcoO109I rG'GnC_Cy
Cuts at: 2163 2510 3058 3059 3813 2163
Size: 347 548 1 754 5024
Fragments arranged by size:
5024 754 548 347 1
EcoRI G'AATT_C
Cuts at: 3678 3678
Size: 6674
EcoRV GAT'ATC
Cuts at: 454 960 454
Size: 506 6168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 183 502 2214 2434 4693 183
Size: 319 1712 220 2259 2164
Fragments arranged by size:
2259 2164 1712 319 220
FspI TGC'GCA
Cuts at: 4455 4455
Size: 6674
HaeII r_GCGC'y
Cuts at: 144 1703 2028 4108 4356 5586 6357 144
Size: 1559 325 2080 248 1230 771 461
Fragments arranged by size:
2080 1559 1230 771 461 325 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 746 778 2131 2163 2637 2669 3382 3414 3594
Size: 32 1353 32 474 32 713 32 180
Cuts at: 3594 3626 4516 4546 4548 4578 746
Size: 32 890 30 2 30 2842
Fragments arranged by size:
2842 1353 890 713 474 180 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 902 1290 2505 3713 902
Size: 388 1215 1208 3863
Fragments arranged by size:
3863 1215 1208 388
KasI G'GCGC_C
Cuts at: 4104 4352 4104
Size: 248 6426
KpnI G_GTAC'C
Cuts at: 1681 3694 1681
Size: 2013 4661
MluI A'CGCG_T
Cuts at: 6107 6107
Size: 6674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1364 5433 5617 1364
Size: 4069 184 2421
Fragments arranged by size:
4069 2421 184
MscI TGG'CCA
Cuts at: 1735 1855 2434 3339 4435 1735
Size: 120 579 905 1096 3974
Fragments arranged by size:
3974 1096 905 579 120
MslI CAynn'nnrTG
Cuts at: 910 953 1190 2476 3013 3072 3470 4663 4790
Size: 43 237 1286 537 59 398 1193 127
Cuts at: 4790 910
Size: 2794
Fragments arranged by size:
2794 1286 1193 537 398 237 127 59
43
MspA1I CmG'CkG
Cuts at: 1746 1880 2316 2700 3785 4099 4459 5243 5488
Size: 134 436 384 1085 314 360 784 245
Cuts at: 5488 1746
Size: 2932
Fragments arranged by size:
2932 1085 784 436 384 360 314 245
134
NaeI GCC'GGC
Cuts at: 534 2498 4855 534
Size: 1964 2357 2353
Fragments arranged by size:
2357 2353 1964
NarI GG'CG_CC
Cuts at: 4105 4353 4105
Size: 248 6426
NcoI C'CATG_G
Cuts at: 2099 2429 2783 3125 2099
Size: 330 354 342 5648
Fragments arranged by size:
5648 354 342 330
NgoMIV G'CCGG_C
Cuts at: 532 2496 4853 532
Size: 1964 2357 2353
Fragments arranged by size:
2357 2353 1964
NheI G'CTAG_C
Cuts at: 528 528
Size: 6674
NspI r_CATG'y
Cuts at: 1743 1782 2481 3426 3727 4227 5830 1743
Size: 39 699 945 301 500 1603 2587
Fragments arranged by size:
2587 1603 945 699 500 301 39
PasI CC'CwG_GG
Cuts at: 1354 3054 1354
Size: 1700 4974
PciI A'CATG_T
Cuts at: 5826 5826
Size: 6674
PflMI CCAn_nnn'nTGG
Cuts at: 267 2034 267
Size: 1767 4907
PfoI T'CCnGG_A
Cuts at: 1970 2120 2288 1970
Size: 150 168 6356
Fragments arranged by size:
6356 168 150
PmeI GTTT'AAAC
Cuts at: 3673 3673
Size: 6674
PmlI CAC'GTG
Cuts at: 3074 3074
Size: 6674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5120 5152 5120
Size: 32 6642
PpuMI rG'GwC_Cy
Cuts at: 2163 2163
Size: 6674
PshAI GACnn'nnGTC
Cuts at: 2595 2595
Size: 6674
PspOMI G'GGCC_C
Cuts at: 3058 3058
Size: 6674
PspXI vC'TCGA_Gb
Cuts at: 2735 2735
Size: 6674
PstI C_TGCA'G
Cuts at: 1751 2321 3138 3721 4406 1751
Size: 570 817 583 685 4019
Fragments arranged by size:
4019 817 685 583 570
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6674
PvuII CAG'CTG
Cuts at: 1746 2316 4099 4459 1746
Size: 570 1783 360 3961
Fragments arranged by size:
3961 1783 570 360
RsrII CG'GwC_CG
Cuts at: 3402 4869 3402
Size: 1467 5207
SacI G_AGCT'C
Cuts at: 920 3688 920
Size: 2768 3906
SalI G'TCGA_C
Cuts at: 900 3711 900
Size: 2811 3863
SapI GCTCTTCn'nnn_
Cuts at: 4697 4907 4697
Size: 210 6464
SbfI CC_TGCA'GG
Cuts at: 3721 3721
Size: 6674
ScaI AGT'ACT
Cuts at: 6191 6191
Size: 6674
SexAI A'CCwGG_T
Cuts at: 515 1895 515
Size: 1380 5294
SfcI C'TryA_G
Cuts at: 33 1623 1747 1764 2317 3134 3468 3717 4402
Size: 1590 124 17 553 817 334 249 685
Cuts at: 4402 5370 5561 33
Size: 968 191 1146
Fragments arranged by size:
1590 1146 968 817 685 553 334 249
191 124 17
SfoI GGC'GCC
Cuts at: 4106 4354 4106
Size: 248 6426
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6674
SmaI CCC'GGG
Cuts at: 2776 3696 2776
Size: 920 5754
SmlI C'TyrA_G
Cuts at: 581 1783 2080 2735 3829 4744 5458 5720 581
Size: 1202 297 655 1094 915 714 262 1535
Fragments arranged by size:
1535 1202 1094 915 714 655 297 262
SpeI A'CTAG_T
Cuts at: 67 2548 67
Size: 2481 4193
SphI G_CATG'C
Cuts at: 3426 3727 4227 3426
Size: 301 500 5873
Fragments arranged by size:
5873 500 301
StuI AGG'CCT
Cuts at: 1324 2678 1324
Size: 1354 5320
StyI C'CwwG_G
Cuts at: 801 2099 2371 2429 2465 2783 3125 3366 3808
Size: 1298 272 58 36 318 342 241 442
Cuts at: 3808 4162 801
Size: 354 3313
Fragments arranged by size:
3313 1298 442 354 342 318 272 241
58 36
SwaI ATTT'AAAT
Cuts at: 1244 1244
Size: 6674
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 321 1543 3067 3390 4257 4390 4849 5058 321
Size: 1222 1524 323 867 133 459 209 1937
Fragments arranged by size:
1937 1524 1222 867 459 323 209 133
TatI w'GTAC_w
Cuts at: 505 719 795 1559 2303 3614 6189 505
Size: 214 76 764 744 1311 2575 990
Fragments arranged by size:
2575 1311 990 764 744 214 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2763 2795 3101 3133 6191 6223 2763
Size: 32 306 32 3058 32 3214
Fragments arranged by size:
3214 3058 306 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4471 4471
Size: 6674
XbaI T'CTAG_A
Cuts at: 2655 3705 2655
Size: 1050 5624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 425 3423 3472 4169 425
Size: 2998 49 697 2930
Fragments arranged by size:
2998 2930 697 49
XhoI C'TCGA_G
Cuts at: 2735 2735
Size: 6674
XmaI C'CCGG_G
Cuts at: 2774 3694 2774
Size: 920 5754
XmnI GAAnn'nnTTC
Cuts at: 1930 2197 3246 1930
Size: 267 1049 5358
Fragments arranged by size:
5358 1049 267
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AlfI AscI AvrII
BfrBI BglI BsiWI BspEI BssHII BstZ17I ClaI CspCI
FseI FspAI HindIII HpaI MfeI NdeI NotI NruI
NsiI PacI PsiI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI ZraI