Restriction map for
pF1KB9486
(Circular) (Six-base) MAPSORT of: pF1KB9486.seq Check: 4583 from: 1 to: 6776
pF1KB9486 6776 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 314 586 3814 314
Size: 272 3228 3276
Fragments arranged by size:
3276 3228 272
Acc65I G'GTAC_C
Cuts at: 746 3792 746
Size: 3046 3730
AclI AA'CG_TT
Cuts at: 6616 6616
Size: 6776
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 488 1496 4601 5033 5386 6048 488
Size: 1008 3105 432 353 662 1216
Fragments arranged by size:
3105 1216 1008 662 432 353
AfeI AGC'GCT
Cuts at: 2575 2575
Size: 6776
AflII C'TTAA_G
Cuts at: 442 442
Size: 6776
AflIII A'CryG_T
Cuts at: 589 1918 4128 5928 6209 589
Size: 1329 2210 1800 281 1156
Fragments arranged by size:
2210 1800 1329 1156 281
AleI CACnn'nnGTG
Cuts at: 1740 1763 1740
Size: 23 6753
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3282 3314 3282
Size: 32 6744
AlwNI CAG_nnn'CTG
Cuts at: 429 660 1200 2451 2869 3646 4081 5519 429
Size: 231 540 1251 418 777 435 1438 1686
Fragments arranged by size:
1686 1438 1251 777 540 435 418 231
ApaI G_GGCC'C
Cuts at: 160 1862 2615 160
Size: 1702 753 4321
Fragments arranged by size:
4321 1702 753
ApaLI G'TGCA_C
Cuts at: 3510 5614 3510
Size: 2104 4672
ApoI r'AATT_y
Cuts at: 631 1558 2182 2639 3780 6129 6385 631
Size: 927 624 457 1141 2349 256 1022
Fragments arranged by size:
2349 1141 1022 927 624 457 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6776
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6776
AvaI C'yCGr_G
Cuts at: 520 1855 3187 3796 520
Size: 1335 1332 609 3500
Fragments arranged by size:
3500 1335 1332 609
BamHI G'GATC_C
Cuts at: 3660 3801 3660
Size: 141 6635
BanI G'GyrC_C
Cuts at: 265 746 1742 2425 3792 4206 4454 4489 265
Size: 481 996 683 1367 414 248 35 2552
Fragments arranged by size:
2552 1367 996 683 481 414 248 35
BanII G_rGCy'C
Cuts at: 160 943 1601 1856 1862 2064 2615 2876 3169
Size: 783 658 255 6 202 551 261 293
Cuts at: 3169 3790 4820 160
Size: 621 1030 2116
Fragments arranged by size:
2116 1030 783 658 621 551 293 261
255 202 6
BbeI G_GCGC'C
Cuts at: 269 2429 4210 4458 269
Size: 2160 1781 248 2587
Fragments arranged by size:
2587 2160 1781 248
BbsI GAAGACnn'nnnn_
Cuts at: 1523 1523
Size: 6776
BbvCI CC'TCA_GC
Cuts at: 653 1564 3704 653
Size: 911 2140 3725
Fragments arranged by size:
3725 2140 911
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3283 3317 3431 3465 3283
Size: 34 114 34 6594
Fragments arranged by size:
6594 114 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4674 5730 6776 4674
Size: 1056 1046 4674
Fragments arranged by size:
4674 1056 1046
BclI T'GATC_A
Cuts at: 1175 1257 2283 4296 1175
Size: 82 1026 2013 3655
Fragments arranged by size:
3655 2013 1026 82
BfrBI ATG'CAT
Cuts at: 811 811
Size: 6776
BglI GCCn_nnn'nGGC
Cuts at: 142 142
Size: 6776
BglII A'GATC_T
Cuts at: 4291 6328 4291
Size: 2037 4739
BlpI GC'TnA_GC
Cuts at: 3888 3888
Size: 6776
Bme1580I G_kGCm'C
Cuts at: 160 1427 1862 2615 3514 4401 4494 5618 160
Size: 1267 435 753 899 887 93 1124 1318
Fragments arranged by size:
1318 1267 1124 899 887 753 435 93
BmrI ACTGGGnnnn_n'
Cuts at: 190 3988 4069 4404 6548 190
Size: 3798 81 335 2144 418
Fragments arranged by size:
3798 2144 418 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5143 5143
Size: 6776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 1184 2448 2538 2760 2972 187
Size: 997 1264 90 222 212 3991
Fragments arranged by size:
3991 1264 997 222 212 90
Bpu10I CC'TnA_GC
Cuts at: 243 653 1564 3218 3704 243
Size: 410 911 1654 486 3315
Fragments arranged by size:
3315 1654 911 486 410
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1537 1830 2810 3564 3952 4831 5545 5843 1537
Size: 293 980 754 388 879 714 298 2470
Fragments arranged by size:
2470 980 879 754 714 388 298 293
BsaI GGTCTCn'nnnn_
Cuts at: 1762 3113 3424 6480 1762
Size: 1351 311 3056 2058
Fragments arranged by size:
3056 2058 1351 311
BsaAI yAC'GTr
Cuts at: 590 708 4129 590
Size: 118 3421 3237
Fragments arranged by size:
3421 3237 118
BsaBI GATnn'nnATC
Cuts at: 6 4290 6327 6
Size: 4284 2037 455
Fragments arranged by size:
4284 2037 455
BsaHI Gr'CG_yC
Cuts at: 266 2426 4207 4455 5157 6653 266
Size: 2160 1781 248 702 1496 389
Fragments arranged by size:
2160 1781 1496 702 389 248
BsaWI w'CCGG_w
Cuts at: 1504 1626 2403 3606 3965 4486 5164 5575 5722
Size: 122 777 1203 359 521 678 411 147
Cuts at: 5722 5996 1504
Size: 274 2284
Fragments arranged by size:
2284 1203 777 678 521 411 359 274
147 122
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 553 583 667 697 2878 2908 3282 3312 3325
Size: 30 84 30 2181 30 374 30 13
Cuts at: 3325 3355 553
Size: 30 3974
Fragments arranged by size:
3974 2181 374 84 30 30 30 30
30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 558 561 745 1943 3145 558
Size: 3 184 1198 1202 4189
Fragments arranged by size:
4189 1202 1198 184 3
BseYI C'CCAG_C
Cuts at: 2551 3453 4201 4959 5624 6032 2551
Size: 902 748 758 665 408 3295
Fragments arranged by size:
3295 902 758 748 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 415 547 2093 2770 3363 382
Size: 33 132 1546 677 593 3795
Fragments arranged by size:
3795 1546 677 593 132 33
BsiEI CG_ry'CG
Cuts at: 104 233 373 4318 4364 5594 104
Size: 129 140 3945 46 1230 1286
Fragments arranged by size:
3945 1286 1230 140 129 46
BsiHKAI G_wGCw'C
Cuts at: 943 1351 1856 2764 3043 3514 3790 4568 4758
Size: 408 505 908 279 471 276 778 190
Cuts at: 4758 5618 943
Size: 860 2101
Fragments arranged by size:
2101 908 860 778 505 471 408 279
276 190
BsmI GAATG_Cn'
Cuts at: 387 3021 387
Size: 2634 4142
BsmBI CGTCTCn'nnnn_
Cuts at: 2617 2772 2617
Size: 155 6621
Bsp1286I G_dGCh'C
Cuts at: 160 943 1351 1427 1601 1856 1862 2064 2615
Size: 783 408 76 174 255 6 202 551
Cuts at: 2615 2764 2876 3043 3169 3514 3790 4401 4494
Size: 149 112 167 126 345 276 611 93
Cuts at: 4494 4568 4758 4820 5618 160
Size: 74 190 62 798 1318
Fragments arranged by size:
1318 798 783 611 551 408 345 276
255 202 190 174 167 149 126 112
93 76 74 62 6
BspEI T'CCGG_A
Cuts at: 1626 1626
Size: 6776
BspHI T'CATG_A
Cuts at: 1413 2286 2696 5208 1413
Size: 873 410 2512 2981
Fragments arranged by size:
2981 2512 873 410
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2445 2736 3442 3826 4040 4342 4723 2445
Size: 291 706 384 214 302 381 4498
Fragments arranged by size:
4498 706 384 381 302 291 214
BsrBI CCG'CTC
Cuts at: 50 5068 5122 6606 6771 50
Size: 5018 54 1484 165 55
Fragments arranged by size:
5018 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 538 2043 2201 3493 4688 538
Size: 1505 158 1292 1195 2626
Fragments arranged by size:
2626 1505 1292 1195 158
BsrFI r'CCGG_y
Cuts at: 3263 3569 4774 4955 3263
Size: 306 1205 181 5084
Fragments arranged by size:
5084 1205 306 181
BsrGI T'GTAC_A
Cuts at: 713 3467 713
Size: 2754 4022
BssSI C'ACGA_G
Cuts at: 1000 5047 5755 1000
Size: 4047 708 2021
Fragments arranged by size:
4047 2021 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3755 4153 3755
Size: 398 6378
BstBI TT'CG_AA
Cuts at: 2481 5137 2481
Size: 2656 4120
BstEII G'GTnAC_C
Cuts at: 694 694
Size: 6776
BstXI CCAn_nnnn'nTGG
Cuts at: 487 2924 487
Size: 2437 4339
BstYI r'GATC_y
Cuts at: 1 737 2972 3660 3801 4268 4291 4626 4872
Size: 736 2235 688 141 467 23 335 246
Cuts at: 4872 5178 5190 5276 6328 1
Size: 306 12 86 1052 449
Fragments arranged by size:
2235 1052 736 688 467 449 335 306
246 141 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1574 1574
Size: 6776
BtgI C'CryG_G
Cuts at: 107 236 750 1872 2097 3499 107
Size: 129 514 1122 225 1402 3384
Fragments arranged by size:
3384 1402 1122 514 225 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1392 4726 4907 5070 1392
Size: 3334 181 163 3098
Fragments arranged by size:
3334 3098 181 163
BtsI GCAGTG_nn'
Cuts at: 1950 2853 3020 3514 4019 1950
Size: 903 167 494 505 4707
Fragments arranged by size:
4707 903 505 494 167
ClaI AT'CG_AT
Cuts at: 2148 2148
Size: 6776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3108 3143 3108
Size: 35 6741
DraI TTT'AAA
Cuts at: 1095 3775 1095
Size: 2680 4096
DraIII CAC_nnn'GTG
Cuts at: 1667 1667
Size: 6776
DrdI GACnn_nn'nnGTC
Cuts at: 4482 5826 4482
Size: 1344 5432
EaeI y'GGCC_r
Cuts at: 191 768 1875 4361 4535 4926 4953 6298 191
Size: 577 1107 2486 174 391 27 1345 669
Fragments arranged by size:
2486 1345 1107 669 577 391 174 27
EagI C'GGCC_G
Cuts at: 4361 4361
Size: 6776
EarI CTCTTCn'nnn_
Cuts at: 2132 3322 3422 4799 5009 2132
Size: 1190 100 1377 210 3899
Fragments arranged by size:
3899 1377 1190 210 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1875 4984 5726 5872 6586 1875
Size: 3109 742 146 714 2065
Fragments arranged by size:
3109 2065 742 714 146
EcoICRI GAG'CTC
Cuts at: 941 1854 3788 941
Size: 913 1934 3929
Fragments arranged by size:
3929 1934 913
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 488 1184 1496 2448 2538 2760 2972 4601
Size: 301 696 312 952 90 222 212 1629
Cuts at: 4601 5033 5386 6048 187
Size: 432 353 662 915
Fragments arranged by size:
1629 952 915 696 662 432 353 312
301 222 212 90
EcoNI CCTnn'n_nnAGG
Cuts at: 918 3160 918
Size: 2242 4534
EcoO109I rG'GnC_Cy
Cuts at: 168 981 1859 2611 3915 168
Size: 813 878 752 1304 3029
Fragments arranged by size:
3029 1304 878 813 752
EcoRI G'AATT_C
Cuts at: 631 3780 631
Size: 3149 3627
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3012 4795 3012
Size: 1783 4993
FseI GG_CCGG'CC
Cuts at: 3573 3573
Size: 6776
FspI TGC'GCA
Cuts at: 4557 4557
Size: 6776
HaeII r_GCGC'y
Cuts at: 202 269 2429 2577 4210 4458 5688 6459 202
Size: 67 2160 148 1633 248 1230 771 519
Fragments arranged by size:
2160 1633 1230 771 519 248 148 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 553 585 819 851 1160 1167 1192 1199 1242
Size: 32 234 32 309 7 25 7 43
Cuts at: 1242 1274 3645 3649 3677 3681 4618 4648 4650
Size: 32 2371 4 28 4 937 30 2
Cuts at: 4650 4680 553
Size: 30 2649
Fragments arranged by size:
2649 2371 937 309 234 43 32 32
32 30 30 28 25 7 7 4
4 2
HincII GTy'rAC
Cuts at: 3815 3815
Size: 6776
KasI G'GCGC_C
Cuts at: 265 2425 4206 4454 265
Size: 2160 1781 248 2587
Fragments arranged by size:
2587 2160 1781 248
KpnI G_GTAC'C
Cuts at: 750 3796 750
Size: 3046 3730
MluI A'CGCG_T
Cuts at: 6209 6209
Size: 6776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5535 5719 5535
Size: 184 6592
MscI TGG'CCA
Cuts at: 193 4537 193
Size: 4344 2432
MslI CAynn'nnrTG
Cuts at: 866 1069 1123 1259 1740 1763 3498 4765 4892
Size: 203 54 136 481 23 1735 1267 127
Cuts at: 4892 866
Size: 2750
Fragments arranged by size:
2750 1735 1267 481 203 136 127 54
23
MspA1I CmG'CkG
Cuts at: 207 238 263 272 744 964 2016 2422 3887
Size: 31 25 9 472 220 1052 406 1465
Cuts at: 3887 4201 4561 5345 5590 207
Size: 314 360 784 245 1393
Fragments arranged by size:
1465 1393 1052 784 472 406 360 314
245 220 31 25 9
NaeI GCC'GGC
Cuts at: 3265 3571 4957 3265
Size: 306 1386 5084
Fragments arranged by size:
5084 1386 306
NarI GG'CG_CC
Cuts at: 266 2426 4207 4455 266
Size: 2160 1781 248 2587
Fragments arranged by size:
2587 2160 1781 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 6776
NgoMIV G'CCGG_C
Cuts at: 3263 3569 4955 3263
Size: 306 1386 5084
Fragments arranged by size:
5084 1386 306
NsiI A_TGCA'T
Cuts at: 813 813
Size: 6776
NspI r_CATG'y
Cuts at: 1922 2936 3829 4329 5932 1922
Size: 1014 893 500 1603 2766
Fragments arranged by size:
2766 1603 1014 893 500
PciI A'CATG_T
Cuts at: 1918 5928 1918
Size: 4010 2766
PfoI T'CCnGG_A
Cuts at: 1912 1940 2444 1912
Size: 28 504 6244
Fragments arranged by size:
6244 504 28
PmeI GTTT'AAAC
Cuts at: 3775 3775
Size: 6776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3282 3314 5222 5254 3282
Size: 32 1908 32 4804
Fragments arranged by size:
4804 1908 32 32
PshAI GACnn'nnGTC
Cuts at: 2629 3105 2629
Size: 476 6300
PsiI TTA'TAA
Cuts at: 2360 2360
Size: 6776
PspOMI G'GGCC_C
Cuts at: 156 1858 2611 156
Size: 1702 753 4321
Fragments arranged by size:
4321 1702 753
PstI C_TGCA'G
Cuts at: 1468 1540 2456 2848 3227 3823 4508 1468
Size: 72 916 392 379 596 685 3736
Fragments arranged by size:
3736 916 685 596 392 379 72
PvuI CG_AT'CG
Cuts at: 104 373 104
Size: 269 6507
PvuII CAG'CTG
Cuts at: 207 964 2016 2422 4201 4561 207
Size: 757 1052 406 1779 360 2422
Fragments arranged by size:
2422 1779 1052 757 406 360
RsrII CG'GwC_CG
Cuts at: 4971 4971
Size: 6776
SacI G_AGCT'C
Cuts at: 943 1856 3790 943
Size: 913 1934 3929
Fragments arranged by size:
3929 1934 913
SacII CC_GC'GG
Cuts at: 239 239
Size: 6776
SalI G'TCGA_C
Cuts at: 3813 3813
Size: 6776
SapI GCTCTTCn'nnn_
Cuts at: 4799 5009 4799
Size: 210 6566
SbfI CC_TGCA'GG
Cuts at: 3823 3823
Size: 6776
ScaI AGT'ACT
Cuts at: 6293 6293
Size: 6776
SfcI C'TryA_G
Cuts at: 33 678 1464 1536 2452 2844 3223 3268 3819
Size: 645 786 72 916 392 379 45 551
Cuts at: 3819 4504 5472 5663 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 916 786 685 645 551 392
379 191 72 45
SfoI GGC'GCC
Cuts at: 267 2427 4208 4456 267
Size: 2160 1781 248 2587
Fragments arranged by size:
2587 2160 1781 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6776
SmaI CCC'GGG
Cuts at: 3798 3798
Size: 6776
SmlI C'TyrA_G
Cuts at: 442 1552 1809 2825 3579 3931 4846 5560 5822
Size: 1110 257 1016 754 352 915 714 262
Cuts at: 5822 442
Size: 1396
Fragments arranged by size:
1396 1110 1016 915 754 714 352 262
257
SpeI A'CTAG_T
Cuts at: 67 2271 67
Size: 2204 4572
SphI G_CATG'C
Cuts at: 3829 4329 3829
Size: 500 6276
SspI AAT'ATT
Cuts at: 2025 2398 3402 2025
Size: 373 1004 5399
Fragments arranged by size:
5399 1004 373
StuI AGG'CCT
Cuts at: 2366 2944 2366
Size: 578 6198
StyI C'CwwG_G
Cuts at: 107 194 1707 2690 3096 3910 4264 107
Size: 87 1513 983 406 814 354 2619
Fragments arranged by size:
2619 1513 983 814 406 354 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 219 3060 3307 4359 4492 4951 5160 219
Size: 2841 247 1052 133 459 209 1835
Fragments arranged by size:
2841 1835 1052 459 247 209 133
TatI w'GTAC_w
Cuts at: 713 2159 2929 3467 6291 713
Size: 1446 770 538 2824 1198
Fragments arranged by size:
2824 1446 1198 770 538
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1788 1820 1908 1940 2904 2936 3122 3154 3325
Size: 32 88 32 964 32 186 32 171
Cuts at: 3325 3357 6293 6325 1788
Size: 32 2936 32 2239
Fragments arranged by size:
2936 2239 964 186 171 88 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3543 4573 3543
Size: 1030 5746
XbaI T'CTAG_A
Cuts at: 3807 3807
Size: 6776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 748 2669 4271 748
Size: 1921 1602 3253
Fragments arranged by size:
3253 1921 1602
XmaI C'CCGG_G
Cuts at: 3796 3796
Size: 6776
XmnI GAAnn'nnTTC
Cuts at: 2995 3355 2995
Size: 360 6416
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AgeI AhdI AlfI AscI AvrII BaeI
BmgBI BmtI BsiWI BssHII BstZ17I EcoRV FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PacI PasI
PflMI PmlI PpuMI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI