Restriction map for
pF1KB9481
(Circular) (Six-base) MAPSORT of: pF1KB9481.seq Check: 2762 from: 1 to: 9758
pF1KB9481 9758 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 781 1815 3299 6623 6796 781
Size: 1034 1484 3324 173 3743
Fragments arranged by size:
3743 3324 1484 1034 173
Acc65I G'GTAC_C
Cuts at: 5021 6774 5021
Size: 1753 8005
AclI AA'CG_TT
Cuts at: 1332 6143 9598 1332
Size: 4811 3455 1492
Fragments arranged by size:
4811 3455 1492
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 830 1666 2384 2841 2916 3730 4019 5284
Size: 695 836 718 457 75 814 289 1265
Cuts at: 5284 5494 5557 6048 7583 8015 8368 9030 135
Size: 210 63 491 1535 432 353 662 863
Fragments arranged by size:
1535 1265 863 836 814 718 695 662
491 457 432 353 289 210 75 63
AflIII A'CryG_T
Cuts at: 3644 7110 8910 9191 3644
Size: 3466 1800 281 4211
Fragments arranged by size:
4211 3466 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 2468 6611 2468
Size: 4143 5615
AleI CACnn'nnGTG
Cuts at: 5126 5230 5989 6463 6473 5126
Size: 104 759 474 10 8411
Fragments arranged by size:
8411 759 474 104 10
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3833 6491 3833
Size: 2658 7100
AlwNI CAG_nnn'CTG
Cuts at: 2422 2953 3223 3751 4021 4285 4354 6399 7063
Size: 531 270 528 270 264 69 2045 664
Cuts at: 7063 8501 2422
Size: 1438 3679
Fragments arranged by size:
3679 2045 1438 664 531 528 270 270
264 69
ApaI G_GGCC'C
Cuts at: 4613 6458 4613
Size: 1845 7913
ApaLI G'TGCA_C
Cuts at: 6466 6568 8596 6466
Size: 102 2028 7628
Fragments arranged by size:
7628 2028 102
ApoI r'AATT_y
Cuts at: 118 899 1235 2121 2130 2317 2391 2509 3416
Size: 781 336 886 9 187 74 118 907
Cuts at: 3416 3913 5455 5542 6762 9111 9367 118
Size: 497 1542 87 1220 2349 256 509
Fragments arranged by size:
2349 1542 1220 907 886 781 509 497
336 256 187 118 87 74 9
AseI AT'TA_AT
Cuts at: 20 20
Size: 9758
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9758
AvaI C'yCGr_G
Cuts at: 6444 6778 6444
Size: 334 9424
AvrII C'CTAG_G
Cuts at: 5424 5424
Size: 9758
BamHI G'GATC_C
Cuts at: 1481 1988 4886 6783 1481
Size: 507 2898 1897 4456
Fragments arranged by size:
4456 2898 1897 507
BanI G'GyrC_C
Cuts at: 375 2337 2760 4058 5021 6103 6774 7188 7436
Size: 1962 423 1298 963 1082 671 414 248
Cuts at: 7436 7471 375
Size: 35 2662
Fragments arranged by size:
2662 1962 1298 1082 963 671 423 414
248 35
BanII G_rGCy'C
Cuts at: 232 910 2075 4097 4152 4613 5606 6458 6772
Size: 678 1165 2022 55 461 993 852 314
Cuts at: 6772 7802 232
Size: 1030 2188
Fragments arranged by size:
2188 2022 1165 1030 993 852 678 461
314 55
BbeI G_GCGC'C
Cuts at: 2764 7192 7440 2764
Size: 4428 248 5082
Fragments arranged by size:
5082 4428 248
BbsI GAAGACnn'nnnn_
Cuts at: 1132 2091 4668 4717 5081 5153 1132
Size: 959 2577 49 364 72 5737
Fragments arranged by size:
5737 2577 959 364 72 49
BbvCI CC'TCA_GC
Cuts at: 2740 2740
Size: 9758
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 371 405 560 594 371
Size: 34 155 34 9535
Fragments arranged by size:
9535 155 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3021 7656 8712 9758 3021
Size: 4635 1056 1046 3021
Fragments arranged by size:
4635 3021 1056 1046
BclI T'GATC_A
Cuts at: 396 3039 7278 396
Size: 2643 4239 2876
Fragments arranged by size:
4239 2876 2643
BfrBI ATG'CAT
Cuts at: 3778 3778
Size: 9758
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 9758
BglII A'GATC_T
Cuts at: 3921 4503 7273 9310 3921
Size: 582 2770 2037 4369
Fragments arranged by size:
4369 2770 2037 582
BlpI GC'TnA_GC
Cuts at: 348 4096 6870 348
Size: 3748 2774 3236
Fragments arranged by size:
3748 3236 2774
Bme1580I G_kGCm'C
Cuts at: 967 4613 5012 6458 6470 6572 7383 7476 8600
Size: 3646 399 1446 12 102 811 93 1124
Cuts at: 8600 967
Size: 2125
Fragments arranged by size:
3646 2125 1446 1124 811 399 102 93
12
BmgBI CAC'GTC
Cuts at: 3633 5898 3633
Size: 2265 7493
BmrI ACTGGGnnnn_n'
Cuts at: 1983 4547 6162 6387 6970 7051 7386 9530 1983
Size: 2564 1615 225 583 81 335 2144 2211
Fragments arranged by size:
2564 2211 2144 1615 583 335 225 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6013 6667 8125 6013
Size: 654 1458 7646
Fragments arranged by size:
7646 1458 654
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 438 783 855 1343 1358 1930 1988 2158
Size: 14 345 72 488 15 572 58 170
Cuts at: 2158 2550 3050 3679 4123 4362 4990 5320 6508
Size: 392 500 629 444 239 628 330 1188
Cuts at: 6508 424
Size: 3674
Fragments arranged by size:
3674 1188 629 628 572 500 488 444
392 345 330 239 170 72 58 15
14
Bpu10I CC'TnA_GC
Cuts at: 2740 2740
Size: 9758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 2584 4136 6934 7813 8527 8825 433
Size: 2151 1552 2798 879 714 298 1366
Fragments arranged by size:
2798 2151 1552 1366 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1943 2267 2566 9462 1943
Size: 324 299 6896 2239
Fragments arranged by size:
6896 2239 324 299
BsaAI yAC'GTr
Cuts at: 4476 4601 7111 4476
Size: 125 2510 7123
Fragments arranged by size:
7123 2510 125
BsaBI GATnn'nnATC
Cuts at: 6 3952 4133 6014 7272 9309 6
Size: 3946 181 1881 1258 2037 455
Fragments arranged by size:
3946 2037 1881 1258 455 181
BsaHI Gr'CG_yC
Cuts at: 2761 7189 7437 8139 9635 2761
Size: 4428 248 702 1496 2884
Fragments arranged by size:
4428 2884 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1168 2722 5789 6947 7468 8146 8557 8704 8978
Size: 1554 3067 1158 521 678 411 147 274
Cuts at: 8978 1168
Size: 1948
Fragments arranged by size:
3067 1948 1554 1158 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 560 590 2696 2726 2960 2990 4429 4459 5302
Size: 30 2106 30 234 30 1439 30 843
Cuts at: 5302 5332 5365 5395 560
Size: 30 33 30 4923
Fragments arranged by size:
4923 2106 1439 843 234 33 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 600 5099 5899 600
Size: 4499 800 4459
Fragments arranged by size:
4499 4459 800
BseYI C'CCAG_C
Cuts at: 4014 4278 6451 7183 7941 8606 9014 4014
Size: 264 2173 732 758 665 408 4758
Fragments arranged by size:
4758 2173 758 732 665 408 264
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 727 5062 287
Size: 440 4335 4983
Fragments arranged by size:
4983 4335 440
BsiEI CG_ry'CG
Cuts at: 104 1815 2727 5051 7300 7346 8576 104
Size: 1711 912 2324 2249 46 1230 1286
Fragments arranged by size:
2324 2249 1711 1286 1230 912 46
BsiHKAI G_wGCw'C
Cuts at: 592 3256 3644 4097 4152 6470 6572 6772 7550
Size: 2664 388 453 55 2318 102 200 778
Cuts at: 7550 7740 8600 592
Size: 190 860 1750
Fragments arranged by size:
2664 2318 1750 860 778 453 388 200
190 102 55
BsmI GAATG_Cn'
Cuts at: 3092 3586 4930 5501 5735 3092
Size: 494 1344 571 234 7115
Fragments arranged by size:
7115 1344 571 494 234
BsmBI CGTCTCn'nnnn_
Cuts at: 2495 3554 2495
Size: 1059 8699
Bsp1286I G_dGCh'C
Cuts at: 232 592 910 967 2075 3256 3644 4097 4152
Size: 360 318 57 1108 1181 388 453 55
Cuts at: 4152 4613 5012 5606 6458 6470 6572 6772 7383
Size: 461 399 594 852 12 102 200 611
Cuts at: 7383 7476 7550 7740 7802 8600 232
Size: 93 74 190 62 798 1390
Fragments arranged by size:
1390 1181 1108 852 798 611 594 461
453 399 388 360 318 200 190 102
93 74 62 57 55 12
BspEI T'CCGG_A
Cuts at: 1168 1168
Size: 9758
BspHI T'CATG_A
Cuts at: 2799 3683 4997 5837 8190 2799
Size: 884 1314 840 2353 4367
Fragments arranged by size:
4367 2353 1314 884 840
BspMI ACCTGCnnnn'nnnn_
Cuts at: 148 397 4850 6808 7022 7324 7705 148
Size: 249 4453 1958 214 302 381 2201
Fragments arranged by size:
4453 2201 1958 381 302 249 214
BsrBI CCG'CTC
Cuts at: 50 347 2943 8050 8104 9588 9753 50
Size: 297 2596 5107 54 1484 165 55
Fragments arranged by size:
5107 2596 1484 297 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2828 3238 4574 4845 4871 5921 7670 2828
Size: 410 1336 271 26 1050 1749 4916
Fragments arranged by size:
4916 1749 1336 1050 410 271 26
BsrFI r'CCGG_y
Cuts at: 1138 1457 4850 5050 7756 7937 1138
Size: 319 3393 200 2706 181 2959
Fragments arranged by size:
3393 2959 2706 319 200 181
BsrGI T'GTAC_A
Cuts at: 3146 3444 4118 4208 3146
Size: 298 674 90 8696
Fragments arranged by size:
8696 674 298 90
BssSI C'ACGA_G
Cuts at: 8029 8737 8029
Size: 708 9050
BstAPI GCAn_nnn'nTGC
Cuts at: 3775 4653 7135 3775
Size: 878 2482 6398
Fragments arranged by size:
6398 2482 878
BstBI TT'CG_AA
Cuts at: 8119 8119
Size: 9758
BstEII G'GTnAC_C
Cuts at: 5785 6162 5785
Size: 377 9381
BstXI CCAn_nnnn'nTGG
Cuts at: 462 1438 4968 5067 462
Size: 976 3530 99 5153
Fragments arranged by size:
5153 3530 976 99
BstYI r'GATC_y
Cuts at: 1 1086 1481 1988 2648 3231 3311 3921 3953
Size: 1085 395 507 660 583 80 610 32
Cuts at: 3953 4503 4738 4807 4886 6732 6783 7250 7273
Size: 550 235 69 79 1846 51 467 23
Cuts at: 7273 7608 7854 8160 8172 8258 9310 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1846 1085 1052 660 610 583 550 507
467 449 395 335 306 246 235 86
80 79 69 51 32 23 12
Bsu36I CC'TnA_GG
Cuts at: 4033 4033
Size: 9758
BtgI C'CryG_G
Cuts at: 107 333 1431 1628 2621 4865 5703 6372 107
Size: 226 1098 197 993 2244 838 669 3493
Fragments arranged by size:
3493 2244 1098 993 838 669 226 197
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 7708 7889 8052 7708
Size: 181 163 9414
Fragments arranged by size:
9414 181 163
BtsI GCAGTG_nn'
Cuts at: 265 945 1280 1880 4654 5259 5483 6468 6480
Size: 680 335 600 2774 605 224 985 12
Cuts at: 6480 6572 7001 265
Size: 92 429 3022
Fragments arranged by size:
3022 2774 985 680 605 600 429 335
224 92 12
ClaI AT'CG_AT
Cuts at: 3381 5955 3381
Size: 2574 7184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4854 4889 4854
Size: 35 9723
DraI TTT'AAA
Cuts at: 1797 6757 1797
Size: 4960 4798
DrdI GACnn_nn'nnGTC
Cuts at: 4165 6376 7464 8808 4165
Size: 2211 1088 1344 5115
Fragments arranged by size:
5115 2211 1344 1088
EaeI y'GGCC_r
Cuts at: 314 2447 4592 4868 5048 6208 6364 7343 7517
Size: 2133 2145 276 180 1160 156 979 174
Cuts at: 7517 7908 7935 9280 314
Size: 391 27 1345 792
Fragments arranged by size:
2145 2133 1345 1160 979 792 391 276
180 174 156 27
EagI C'GGCC_G
Cuts at: 5048 7343 5048
Size: 2295 7463
EarI CTCTTCn'nnn_
Cuts at: 333 806 1055 2002 2403 2658 3328 6063 7781
Size: 473 249 947 401 255 670 2735 1718
Cuts at: 7781 7991 333
Size: 210 2100
Fragments arranged by size:
2735 2100 1718 947 670 473 401 255
249 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 841 871 3750 6331 7966 8708 8854 9568 841
Size: 30 2879 2581 1635 742 146 714 1031
Fragments arranged by size:
2879 2581 1635 1031 742 714 146 30
EcoICRI GAG'CTC
Cuts at: 4095 4150 6770 4095
Size: 55 2620 7083
Fragments arranged by size:
7083 2620 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 135 424 438 783 830 855 1343 1358 1666
Size: 289 14 345 47 25 488 15 308
Cuts at: 1666 1930 1988 2158 2384 2550 2841 2916 3050
Size: 264 58 170 226 166 291 75 134
Cuts at: 3050 3679 3730 4019 4123 4362 4990 5284 5320
Size: 629 51 289 104 239 628 294 36
Cuts at: 5320 5494 5557 6048 6508 7583 8015 8368 9030
Size: 174 63 491 460 1075 432 353 662
Cuts at: 9030 135
Size: 863
Fragments arranged by size:
1075 863 662 629 628 491 488 460
432 353 345 308 294 291 289 289
264 239 226 174 170 166 134 104
75 63 58 51 47 36 25 15
14
EcoO109I rG'GnC_Cy
Cuts at: 3704 4428 4609 4610 5532 6240 6897 3704
Size: 724 181 1 922 708 657 6565
Fragments arranged by size:
6565 922 724 708 657 181 1
EcoRI G'AATT_C
Cuts at: 2130 2317 5542 6762 2130
Size: 187 3225 1220 5126
Fragments arranged by size:
5126 3225 1220 187
EcoRV GAT'ATC
Cuts at: 3843 3843
Size: 9758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 427 3651 6132 7777 427
Size: 3224 2481 1645 2408
Fragments arranged by size:
3224 2481 2408 1645
FspI TGC'GCA
Cuts at: 3244 3877 7539 3244
Size: 633 3662 5463
Fragments arranged by size:
5463 3662 633
HaeII r_GCGC'y
Cuts at: 2764 7192 7440 8670 9441 2764
Size: 4428 248 1230 771 3081
Fragments arranged by size:
4428 3081 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 449 481 717 749 1035 1067 1923 1955 2454
Size: 32 236 32 286 32 856 32 499
Cuts at: 2454 2486 3827 3859 4212 4244 4728 4760 5622
Size: 32 1341 32 353 32 484 32 862
Cuts at: 5622 5654 5691 5723 6007 6039 6597 6629 6722
Size: 32 37 32 284 32 558 32 93
Cuts at: 6722 6754 7600 7630 7632 7662 449
Size: 32 846 30 2 30 2545
Fragments arranged by size:
2545 1341 862 856 846 558 499 484
353 286 284 236 93 37 32 32
32 32 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 513 782 1816 3300 3609 4158 5181 6797 513
Size: 269 1034 1484 309 549 1023 1616 3474
Fragments arranged by size:
3474 1616 1484 1034 1023 549 309 269
HindIII A'AGCT_T
Cuts at: 5401 5401
Size: 9758
KasI G'GCGC_C
Cuts at: 2760 7188 7436 2760
Size: 4428 248 5082
Fragments arranged by size:
5082 4428 248
KpnI G_GTAC'C
Cuts at: 5025 6778 5025
Size: 1753 8005
MluI A'CGCG_T
Cuts at: 9191 9191
Size: 9758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 347 5882 8517 8701 347
Size: 5535 2635 184 1404
Fragments arranged by size:
5535 2635 1404 184
MscI TGG'CCA
Cuts at: 316 2449 4594 6210 6366 7519 316
Size: 2133 2145 1616 156 1153 2555
Fragments arranged by size:
2555 2145 2133 1616 1153 156
MslI CAynn'nnrTG
Cuts at: 381 996 1496 1570 2029 2195 2537 4999 5038
Size: 615 500 74 459 166 342 2462 39
Cuts at: 5038 5126 5128 5230 5326 5659 5989 6157 6463
Size: 88 2 102 96 333 330 168 306
Cuts at: 6463 6473 6635 7747 7874 381
Size: 10 162 1112 127 2265
Fragments arranged by size:
2462 2265 1112 615 500 459 342 333
330 306 168 166 162 127 102 96
88 74 39 10 2
MspA1I CmG'CkG
Cuts at: 335 365 1315 1838 3318 3701 4021 4073 4100
Size: 30 950 523 1480 383 320 52 27
Cuts at: 4100 4525 5189 6214 6869 7183 7543 8327 8572
Size: 425 664 1025 655 314 360 784 245
Cuts at: 8572 335
Size: 1521
Fragments arranged by size:
1521 1480 1025 950 784 664 655 523
425 383 360 320 314 245 52 30
27
NaeI GCC'GGC
Cuts at: 4852 5052 7939 4852
Size: 200 2887 6671
Fragments arranged by size:
6671 2887 200
NarI GG'CG_CC
Cuts at: 2761 7189 7437 2761
Size: 4428 248 5082
Fragments arranged by size:
5082 4428 248
NcoI C'CATG_G
Cuts at: 107 1431 1628 5703 6372 107
Size: 1324 197 4075 669 3493
Fragments arranged by size:
4075 3493 1324 669 197
NdeI CA'TA_TG
Cuts at: 1185 1185
Size: 9758
NgoMIV G'CCGG_C
Cuts at: 4850 5050 7937 4850
Size: 200 2887 6671
Fragments arranged by size:
6671 2887 200
NsiI A_TGCA'T
Cuts at: 3780 3780
Size: 9758
NspI r_CATG'y
Cuts at: 1150 1516 2194 2446 3025 3271 5658 6754 6811
Size: 366 678 252 579 246 2387 1096 57
Cuts at: 6811 7311 8914 1150
Size: 500 1603 1994
Fragments arranged by size:
2387 1994 1603 1096 678 579 500 366
252 246 57
PasI CC'CwG_GG
Cuts at: 948 948
Size: 9758
PciI A'CATG_T
Cuts at: 8910 8910
Size: 9758
PflMI CCAn_nnn'nTGG
Cuts at: 3657 4354 4601 4804 6373 3657
Size: 697 247 203 1569 7042
Fragments arranged by size:
7042 1569 697 247 203
PfoI T'CCnGG_A
Cuts at: 1701 4139 4461 4777 6290 1701
Size: 2438 322 316 1513 5169
Fragments arranged by size:
5169 2438 1513 322 316
PmeI GTTT'AAAC
Cuts at: 6757 6757
Size: 9758
PmlI CAC'GTG
Cuts at: 4601 4601
Size: 9758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3631 3663 8204 8236 3631
Size: 32 4541 32 5153
Fragments arranged by size:
5153 4541 32 32
PpuMI rG'GwC_Cy
Cuts at: 3704 4428 5532 6240 3704
Size: 724 1104 708 7222
Fragments arranged by size:
7222 1104 724 708
PspOMI G'GGCC_C
Cuts at: 4609 6454 4609
Size: 1845 7913
PstI C_TGCA'G
Cuts at: 699 1587 4649 5188 5689 6254 6475 6805 7490
Size: 888 3062 539 501 565 221 330 685
Cuts at: 7490 699
Size: 2967
Fragments arranged by size:
3062 2967 888 685 565 539 501 330
221
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9758
PvuII CAG'CTG
Cuts at: 1315 1838 3318 4021 4100 5189 7183 7543 1315
Size: 523 1480 703 79 1089 1994 360 3530
Fragments arranged by size:
3530 1994 1480 1089 703 523 360 79
RsrII CG'GwC_CG
Cuts at: 7953 7953
Size: 9758
SacI G_AGCT'C
Cuts at: 4097 4152 6772 4097
Size: 55 2620 7083
Fragments arranged by size:
7083 2620 55
SacII CC_GC'GG
Cuts at: 336 336
Size: 9758
SalI G'TCGA_C
Cuts at: 780 1814 3298 6795 780
Size: 1034 1484 3497 3743
Fragments arranged by size:
3743 3497 1484 1034
SanDI GG'GwC_CC
Cuts at: 3704 3704
Size: 9758
SapI GCTCTTCn'nnn_
Cuts at: 3328 7781 7991 3328
Size: 4453 210 5095
Fragments arranged by size:
5095 4453 210
SbfI CC_TGCA'GG
Cuts at: 6805 6805
Size: 9758
ScaI AGT'ACT
Cuts at: 1248 9275 1248
Size: 8027 1731
SexAI A'CCwGG_T
Cuts at: 2333 5024 2333
Size: 2691 7067
SfcI C'TryA_G
Cuts at: 33 695 705 933 1583 1599 2231 2360 4645
Size: 662 10 228 650 16 632 129 2285
Cuts at: 4645 5184 5490 5685 5845 6250 6471 6801 7486
Size: 539 306 195 160 405 221 330 685
Cuts at: 7486 8454 8645 33
Size: 968 191 1146
Fragments arranged by size:
2285 1146 968 685 662 650 632 539
405 330 306 228 221 195 191 160
129 16 10
SfoI GGC'GCC
Cuts at: 2762 7190 7438 2762
Size: 4428 248 5082
Fragments arranged by size:
5082 4428 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9758
SmaI CCC'GGG
Cuts at: 6446 6780 6446
Size: 334 9424
SmlI C'TyrA_G
Cuts at: 412 2599 4151 6913 7828 8542 8804 412
Size: 2187 1552 2762 915 714 262 1366
Fragments arranged by size:
2762 2187 1552 1366 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1611 4164 67
Size: 1544 2553 5661
Fragments arranged by size:
5661 2553 1544
SphI G_CATG'C
Cuts at: 3025 5658 6811 7311 3025
Size: 2633 1153 500 5472
Fragments arranged by size:
5472 2633 1153 500
SspI AAT'ATT
Cuts at: 1302 3498 1302
Size: 2196 7562
StuI AGG'CCT
Cuts at: 679 868 3974 4454 679
Size: 189 3106 480 5983
Fragments arranged by size:
5983 3106 480 189
StyI C'CwwG_G
Cuts at: 107 863 1431 1628 1774 2145 2454 3756 4589
Size: 756 568 197 146 371 309 1302 833
Cuts at: 4589 5424 5703 5873 6107 6372 6892 7246 107
Size: 835 279 170 234 265 520 354 2619
Fragments arranged by size:
2619 1302 835 833 756 568 520 371
354 309 279 265 234 197 170 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3006 4473 4957 6406 6530 7341 7474 7933 8142
Size: 1467 484 1449 124 811 133 459 209
Cuts at: 8142 3006
Size: 4622
Fragments arranged by size:
4622 1467 1449 811 484 459 209 133
124
TatI w'GTAC_w
Cuts at: 1246 2615 3146 3444 4118 4208 5303 9273 1246
Size: 1369 531 298 674 90 1095 3970 1731
Fragments arranged by size:
3970 1731 1369 1095 674 531 298 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1349 1381 2402 2434 2696 2728 3537 3569 4700
Size: 32 1021 32 262 32 809 32 1131
Cuts at: 4700 4732 5302 5334 9275 9307 1349
Size: 32 570 32 3941 32 1800
Fragments arranged by size:
3941 1800 1131 1021 809 570 262 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2959 3276 7555 2959
Size: 317 4279 5162
Fragments arranged by size:
5162 4279 317
XbaI T'CTAG_A
Cuts at: 6789 6789
Size: 9758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6546 7253 6546
Size: 707 9051
XmaI C'CCGG_G
Cuts at: 6444 6778 6444
Size: 334 9424
XmnI GAAnn'nnTTC
Cuts at: 167 1332 5221 5542 167
Size: 1165 3889 321 4383
Fragments arranged by size:
4383 3889 1165 321
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AloI AscI BaeI
BmtI BsiWI BssHII BstZ17I DraIII EcoNI FseI FspAI
HpaI MfeI NheI NotI NruI PacI PshAI PsiI
PspXI PsrI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI