Restriction map for
pF1KB9478
(Circular) (Six-base) MAPSORT of: pF1KB9478.seq Check: 6658 from: 1 to: 6746
pF1KB9478 6746 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 3161 3784 3161
Size: 623 6123
Acc65I G'GTAC_C
Cuts at: 3762 3762
Size: 6746
AclI AA'CG_TT
Cuts at: 6586 6586
Size: 6746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 1207 1372 4571 5003 5356 6018 1141
Size: 66 165 3199 432 353 662 1869
Fragments arranged by size:
3199 1869 662 432 353 165 66
AflII C'TTAA_G
Cuts at: 2752 2752
Size: 6746
AflIII A'CryG_T
Cuts at: 284 4098 5898 6179 284
Size: 3814 1800 281 851
Fragments arranged by size:
3814 1800 851 281
AleI CACnn'nnGTG
Cuts at: 434 3646 434
Size: 3212 3534
AlwNI CAG_nnn'CTG
Cuts at: 582 3127 3172 4051 5489 582
Size: 2545 45 879 1438 1839
Fragments arranged by size:
2545 1839 1438 879 45
ApaI G_GGCC'C
Cuts at: 1702 1827 2242 1702
Size: 125 415 6206
Fragments arranged by size:
6206 415 125
ApaLI G'TGCA_C
Cuts at: 3651 3688 5584 3651
Size: 37 1896 4813
Fragments arranged by size:
4813 1896 37
ApoI r'AATT_y
Cuts at: 1347 3187 3622 3750 6099 6355 1347
Size: 1840 435 128 2349 256 1738
Fragments arranged by size:
2349 1840 1738 435 256 128
AseI AT'TA_AT
Cuts at: 20 20
Size: 6746
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6746
AvaI C'yCGr_G
Cuts at: 1820 1946 2009 2060 2437 3766 1820
Size: 126 63 51 377 1329 4800
Fragments arranged by size:
4800 1329 377 126 63 51
BamHI G'GATC_C
Cuts at: 3771 3771
Size: 6746
BanI G'GyrC_C
Cuts at: 1727 3375 3762 4176 4424 4459 1727
Size: 1648 387 414 248 35 4014
Fragments arranged by size:
4014 1648 414 387 248 35
BanII G_rGCy'C
Cuts at: 239 1598 1702 1718 1827 1889 2131 2242 2518
Size: 1359 104 16 109 62 242 111 276
Cuts at: 2518 2875 3760 4790 239
Size: 357 885 1030 2195
Fragments arranged by size:
2195 1359 1030 885 357 276 242 111
109 104 62 16
BbeI G_GCGC'C
Cuts at: 4180 4428 4180
Size: 248 6498
BbsI GAAGACnn'nnnn_
Cuts at: 1385 2050 1385
Size: 665 6081
BciVI GTATCCnnnnn_n'
Cuts at: 2417 4644 5700 6746 2417
Size: 2227 1056 1046 2417
Fragments arranged by size:
2417 2227 1056 1046
BclI T'GATC_A
Cuts at: 4266 4266
Size: 6746
BfrBI ATG'CAT
Cuts at: 3260 3389 3260
Size: 129 6617
BglI GCCn_nnn'nGGC
Cuts at: 1722 1822 2667 1722
Size: 100 845 5801
Fragments arranged by size:
5801 845 100
BglII A'GATC_T
Cuts at: 3177 4261 6298 3177
Size: 1084 2037 3625
Fragments arranged by size:
3625 2037 1084
BlpI GC'TnA_GC
Cuts at: 1247 3637 3858 1247
Size: 2390 221 4135
Fragments arranged by size:
4135 2390 221
Bme1580I G_kGCm'C
Cuts at: 1702 1827 2099 2242 3655 3692 4371 4464 5588
Size: 125 272 143 1413 37 679 93 1124
Cuts at: 5588 1702
Size: 2860
Fragments arranged by size:
2860 1413 1124 679 272 143 125 93
37
BmrI ACTGGGnnnn_n'
Cuts at: 111 721 3453 3508 3958 4039 4374 6518 111
Size: 610 2732 55 450 81 335 2144 339
Fragments arranged by size:
2732 2144 610 450 339 335 81 55
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5113 5113
Size: 6746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 671 1791 2088 2614 2649 2859 671
Size: 1120 297 526 35 210 4558
Fragments arranged by size:
4558 1120 526 297 210 35
Bpu10I CC'TnA_GC
Cuts at: 1622 2201 2362 1622
Size: 579 161 6006
Fragments arranged by size:
6006 579 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 869 2511 2957 3922 4801 5515 5813 869
Size: 1642 446 965 879 714 298 1802
Fragments arranged by size:
1802 1642 965 879 714 446 298
BsaI GGTCTCn'nnnn_
Cuts at: 650 871 2006 6450 650
Size: 221 1135 4444 946
Fragments arranged by size:
4444 1135 946 221
BsaAI yAC'GTr
Cuts at: 4099 4099
Size: 6746
BsaBI GATnn'nnATC
Cuts at: 6 4260 6297 6
Size: 4254 2037 455
Fragments arranged by size:
4254 2037 455
BsaHI Gr'CG_yC
Cuts at: 4177 4425 5127 6623 4177
Size: 248 702 1496 4300
Fragments arranged by size:
4300 1496 702 248
BsaWI w'CCGG_w
Cuts at: 567 1614 2905 3935 4456 5134 5545 5692 5966
Size: 1047 1291 1030 521 678 411 147 274
Cuts at: 5966 567
Size: 1347
Fragments arranged by size:
1347 1291 1047 1030 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1596 1626 2113 2143 1596
Size: 30 487 30 6199
Fragments arranged by size:
6199 487 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1434 1869 2363 2526 2664 2874 1434
Size: 435 494 163 138 210 5306
Fragments arranged by size:
5306 494 435 210 163 138
BseYI C'CCAG_C
Cuts at: 831 908 1608 2429 4171 4929 5594 6002 831
Size: 77 700 821 1742 758 665 408 1575
Fragments arranged by size:
1742 1575 821 758 700 665 408 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1436 1532 2732 1436
Size: 96 1200 5450
Fragments arranged by size:
5450 1200 96
BsiEI CG_ry'CG
Cuts at: 104 4288 4334 5564 104
Size: 4184 46 1230 1286
Fragments arranged by size:
4184 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1467 1711 2131 2207 2518 2894 3655 3692 3760
Size: 244 420 76 311 376 761 37 68
Cuts at: 3760 4538 4728 5588 1467
Size: 778 190 860 2625
Fragments arranged by size:
2625 860 778 761 420 376 311 244
190 76 68 37
BsmI GAATG_Cn'
Cuts at: 410 1773 410
Size: 1363 5383
Bsp1286I G_dGCh'C
Cuts at: 239 1467 1598 1702 1711 1718 1827 1889 2099
Size: 1228 131 104 9 7 109 62 210
Cuts at: 2099 2131 2207 2242 2518 2875 2894 3655 3692
Size: 32 76 35 276 357 19 761 37
Cuts at: 3692 3760 4371 4464 4538 4728 4790 5588 239
Size: 68 611 93 74 190 62 798 1397
Fragments arranged by size:
1397 1228 798 761 611 357 276 210
190 131 109 104 93 76 74 68
62 62 37 35 32 19 9 7
BspEI T'CCGG_A
Cuts at: 1614 2905 1614
Size: 1291 5455
BspHI T'CATG_A
Cuts at: 5178 5178
Size: 6746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 155 1942 2420 3796 4010 4312 4693 155
Size: 1787 478 1376 214 302 381 2208
Fragments arranged by size:
2208 1787 1376 478 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2361 5038 5092 6576 6741 50
Size: 2311 2677 54 1484 165 55
Fragments arranged by size:
2677 2311 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 968 2326 3389 4658 968
Size: 1358 1063 1269 3056
Fragments arranged by size:
3056 1358 1269 1063
BsrFI r'CCGG_y
Cuts at: 4744 4925 4744
Size: 181 6565
BsrGI T'GTAC_A
Cuts at: 128 128
Size: 6746
BssSI C'ACGA_G
Cuts at: 1761 3606 5017 5725 1761
Size: 1845 1411 708 2782
Fragments arranged by size:
2782 1845 1411 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3266 4123 3266
Size: 857 5889
BstBI TT'CG_AA
Cuts at: 153 5107 153
Size: 4954 1792
BstEII G'GTnAC_C
Cuts at: 637 637
Size: 6746
BstYI r'GATC_y
Cuts at: 1 1788 3177 3771 4238 4261 4596 4842 5148
Size: 1787 1389 594 467 23 335 246 306
Cuts at: 5148 5160 5246 6298 1
Size: 12 86 1052 449
Fragments arranged by size:
1787 1389 1052 594 467 449 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 977 995 1720 2288 107
Size: 870 18 725 568 4565
Fragments arranged by size:
4565 870 725 568 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 325 3304 4696 4877 5040 325
Size: 2979 1392 181 163 2031
Fragments arranged by size:
2979 2031 1392 181 163
BtsI GCAGTG_nn'
Cuts at: 863 1042 1515 2099 3480 3492 3989 863
Size: 179 473 584 1381 12 497 3620
Fragments arranged by size:
3620 1381 584 497 473 179 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2369 2404 2369
Size: 35 6711
DraI TTT'AAA
Cuts at: 3745 3745
Size: 6746
DrdI GACnn_nn'nnGTC
Cuts at: 4452 5796 4452
Size: 1344 5402
EaeI y'GGCC_r
Cuts at: 1207 2537 2784 4331 4505 4896 4923 6268 1207
Size: 1330 247 1547 174 391 27 1345 1685
Fragments arranged by size:
1685 1547 1345 1330 391 247 174 27
EagI C'GGCC_G
Cuts at: 4331 4331
Size: 6746
EarI CTCTTCn'nnn_
Cuts at: 639 773 1172 2503 2841 2850 4769 4979 639
Size: 134 399 1331 338 9 1919 210 2406
Fragments arranged by size:
2406 1919 1331 399 338 210 134 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4954 5696 5842 6556 4954
Size: 742 146 714 5144
Fragments arranged by size:
5144 742 714 146
EcoICRI GAG'CTC
Cuts at: 2129 2516 3758 2129
Size: 387 1242 5117
Fragments arranged by size:
5117 1242 387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 671 1141 1207 1372 1791 2088 2614 2649 2859
Size: 470 66 165 419 297 526 35 210
Cuts at: 2859 4571 5003 5356 6018 671
Size: 1712 432 353 662 1399
Fragments arranged by size:
1712 1399 662 526 470 432 419 353
297 210 165 66 35
EcoNI CCTnn'n_nnAGG
Cuts at: 398 586 601 398
Size: 188 15 6543
Fragments arranged by size:
6543 188 15
EcoO109I rG'GnC_Cy
Cuts at: 849 1014 1698 1699 1824 2195 2238 2669 2790
Size: 165 684 1 125 371 43 431 121
Cuts at: 2790 3455 3600 3885 849
Size: 665 145 285 3710
Fragments arranged by size:
3710 684 665 431 371 285 165 145
125 121 43 1
EcoRI G'AATT_C
Cuts at: 3622 3750 3622
Size: 128 6618
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1402 3019 4765 1402
Size: 1617 1746 3383
Fragments arranged by size:
3383 1746 1617
FspI TGC'GCA
Cuts at: 4527 4527
Size: 6746
HaeII r_GCGC'y
Cuts at: 3229 4180 4428 5658 6429 3229
Size: 951 248 1230 771 3546
Fragments arranged by size:
3546 1230 951 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 250 282 576 608 1116 1148 1531 1563 1845
Size: 32 294 32 508 32 383 32 282
Cuts at: 1845 1877 2401 2433 4588 4618 4620 4650 250
Size: 32 524 32 2155 30 2 30 2346
Fragments arranged by size:
2346 2155 524 508 383 294 282 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3785 3785
Size: 6746
HindIII A'AGCT_T
Cuts at: 2385 2385
Size: 6746
KasI G'GCGC_C
Cuts at: 4176 4424 4176
Size: 248 6498
KpnI G_GTAC'C
Cuts at: 3766 3766
Size: 6746
MluI A'CGCG_T
Cuts at: 6179 6179
Size: 6746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 779 1654 1887 3042 5505 5689 779
Size: 875 233 1155 2463 184 1836
Fragments arranged by size:
2463 1836 1155 875 233 184
MscI TGG'CCA
Cuts at: 2539 2786 4507 2539
Size: 247 1721 4778
Fragments arranged by size:
4778 1721 247
MslI CAynn'nnrTG
Cuts at: 434 3289 3646 4735 4862 434
Size: 2855 357 1089 127 2318
Fragments arranged by size:
2855 2318 1089 357 127
MspA1I CmG'CkG
Cuts at: 1246 1650 1684 2258 3857 4171 4531 5315 5560
Size: 404 34 574 1599 314 360 784 245
Cuts at: 5560 1246
Size: 2432
Fragments arranged by size:
2432 1599 784 574 404 360 314 245
34
NaeI GCC'GGC
Cuts at: 4927 4927
Size: 6746
NarI GG'CG_CC
Cuts at: 4177 4425 4177
Size: 248 6498
NcoI C'CATG_G
Cuts at: 107 995 107
Size: 888 5858
NgoMIV G'CCGG_C
Cuts at: 4925 4925
Size: 6746
NsiI A_TGCA'T
Cuts at: 3262 3391 3262
Size: 129 6617
NspI r_CATG'y
Cuts at: 288 1291 3264 3799 4299 5902 288
Size: 1003 1973 535 500 1603 1132
Fragments arranged by size:
1973 1603 1132 1003 535 500
PciI A'CATG_T
Cuts at: 284 5898 284
Size: 5614 1132
PflMI CCAn_nnn'nTGG
Cuts at: 2289 2289
Size: 6746
PfoI T'CCnGG_A
Cuts at: 2297 2297
Size: 6746
PmeI GTTT'AAAC
Cuts at: 3745 3745
Size: 6746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5192 5224 5192
Size: 32 6714
PpuMI rG'GwC_Cy
Cuts at: 849 2195 2790 3455 849
Size: 1346 595 665 4140
Fragments arranged by size:
4140 1346 665 595
PshAI GACnn'nnGTC
Cuts at: 1669 1669
Size: 6746
PspOMI G'GGCC_C
Cuts at: 1698 1823 2238 1698
Size: 125 415 6206
Fragments arranged by size:
6206 415 125
PstI C_TGCA'G
Cuts at: 1973 3793 4478 1973
Size: 1820 685 4241
Fragments arranged by size:
4241 1820 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6746
PvuII CAG'CTG
Cuts at: 1246 1650 1684 4171 4531 1246
Size: 404 34 2487 360 3461
Fragments arranged by size:
3461 2487 404 360 34
RsrII CG'GwC_CG
Cuts at: 4941 4941
Size: 6746
SacI G_AGCT'C
Cuts at: 2131 2518 3760 2131
Size: 387 1242 5117
Fragments arranged by size:
5117 1242 387
SalI G'TCGA_C
Cuts at: 3783 3783
Size: 6746
SanDI GG'GwC_CC
Cuts at: 3455 3455
Size: 6746
SapI GCTCTTCn'nnn_
Cuts at: 639 4769 4979 639
Size: 4130 210 2406
Fragments arranged by size:
4130 2406 210
SbfI CC_TGCA'GG
Cuts at: 1973 3793 1973
Size: 1820 4926
ScaI AGT'ACT
Cuts at: 324 6263 324
Size: 5939 807
SexAI A'CCwGG_T
Cuts at: 3683 3683
Size: 6746
SfcI C'TryA_G
Cuts at: 33 1969 3173 3789 4474 5442 5633 33
Size: 1936 1204 616 685 968 191 1146
Fragments arranged by size:
1936 1204 1146 968 685 616 191
SfoI GGC'GCC
Cuts at: 4178 4426 4178
Size: 248 6498
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6746
SmaI CCC'GGG
Cuts at: 1822 1948 2062 2439 3768 1822
Size: 126 114 377 1329 4800
Fragments arranged by size:
4800 1329 377 126 114
SmlI C'TyrA_G
Cuts at: 884 2526 2752 2972 3901 4816 5530 5792 884
Size: 1642 226 220 929 915 714 262 1838
Fragments arranged by size:
1838 1642 929 915 714 262 226 220
SpeI A'CTAG_T
Cuts at: 67 433 2269 67
Size: 366 1836 4544
Fragments arranged by size:
4544 1836 366
SphI G_CATG'C
Cuts at: 3264 3799 4299 3264
Size: 535 500 5711
Fragments arranged by size:
5711 535 500
SrfI GCCC'GGGC
Cuts at: 1948 1948
Size: 6746
SspI AAT'ATT
Cuts at: 3245 3245
Size: 6746
StuI AGG'CCT
Cuts at: 1939 1939
Size: 6746
StyI C'CwwG_G
Cuts at: 107 597 845 995 2036 3656 3880 4234 107
Size: 490 248 150 1041 1620 224 354 2619
Fragments arranged by size:
2619 1620 1041 490 354 248 224 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 656 1745 4329 4462 4921 5130 656
Size: 1089 2584 133 459 209 2272
Fragments arranged by size:
2584 2272 1089 459 209 133
TatI w'GTAC_w
Cuts at: 128 322 1240 6261 128
Size: 194 918 5021 613
Fragments arranged by size:
5021 918 613 194
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 433 465 2113 2145 6263 6295 433
Size: 32 1648 32 4118 32 884
Fragments arranged by size:
4118 1648 884 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4543 4543
Size: 6746
XbaI T'CTAG_A
Cuts at: 3777 3777
Size: 6746
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 595 3282 3466 4241 595
Size: 2687 184 775 3100
Fragments arranged by size:
3100 2687 775 184
XmaI C'CCGG_G
Cuts at: 1820 1946 2060 2437 3766 1820
Size: 126 114 377 1329 4800
Fragments arranged by size:
4800 1329 377 126 114
XmnI GAAnn'nnTTC
Cuts at: 218 313 1181 218
Size: 95 868 5783
Fragments arranged by size:
5783 868 95
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BmgBI BmtI BsiWI BsmBI
BssHII BstXI BstZ17I Bsu36I ClaI DraIII EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI PacI
PasI PmlI PsiI PspXI PsrI SacII SfiI SgrAI
SnaBI SwaI XhoI ZraI