Restriction map for
pF1KB9477
(Circular) (Six-base) MAPSORT of: pF1KB9477.seq Check: 2400 from: 1 to: 4112
pF1KB9477 4112 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AatII G_ACGT'C
Cuts at: 1070 1070
Size: 4112
AccI GT'mk_AC
Cuts at: 1150 1150
Size: 4112
Acc65I G'GTAC_C
Cuts at: 1128 1128
Size: 4112
AclI AA'CG_TT
Cuts at: 3952 3952
Size: 4112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1937 2369 2722 3384 754
Size: 1183 432 353 662 1482
Fragments arranged by size:
1482 1183 662 432 353
AflIII A'CryG_T
Cuts at: 1464 3264 3545 1464
Size: 1800 281 2031
Fragments arranged by size:
2031 1800 281
AleI CACnn'nnGTG
Cuts at: 967 967
Size: 4112
AlwNI CAG_nnn'CTG
Cuts at: 663 1417 2855 663
Size: 754 1438 1920
Fragments arranged by size:
1920 1438 754
ApaI G_GGCC'C
Cuts at: 235 856 235
Size: 621 3491
ApaLI G'TGCA_C
Cuts at: 2950 2950
Size: 4112
ApoI r'AATT_y
Cuts at: 1116 3465 3721 1116
Size: 2349 256 1507
Fragments arranged by size:
2349 1507 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4112
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
AvaI C'yCGr_G
Cuts at: 535 713 848 1132 535
Size: 178 135 284 3515
Fragments arranged by size:
3515 284 178 135
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 921 954 921
Size: 33 4079
BamHI G'GATC_C
Cuts at: 1137 1137
Size: 4112
BanI G'GyrC_C
Cuts at: 138 1075 1128 1542 1790 1825 138
Size: 937 53 414 248 35 2425
Fragments arranged by size:
2425 937 414 248 53 35
BanII G_rGCy'C
Cuts at: 235 542 856 1126 2156 235
Size: 307 314 270 1030 2191
Fragments arranged by size:
2191 1030 314 307 270
BbeI G_GCGC'C
Cuts at: 142 1079 1546 1794 142
Size: 937 467 248 2460
Fragments arranged by size:
2460 937 467 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 585 619 741 775 90
Size: 34 461 34 122 34 3427
Fragments arranged by size:
3427 461 122 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2010 3066 4112 2010
Size: 1056 1046 2010
Fragments arranged by size:
2010 1056 1046
BclI T'GATC_A
Cuts at: 767 1632 767
Size: 865 3247
BglI GCCn_nnn'nGGC
Cuts at: 668 797 668
Size: 129 3983
BglII A'GATC_T
Cuts at: 1627 3664 1627
Size: 2037 2075
BlpI GC'TnA_GC
Cuts at: 167 1224 167
Size: 1057 3055
Bme1580I G_kGCm'C
Cuts at: 235 820 856 1737 1830 2954 235
Size: 585 36 881 93 1124 1393
Fragments arranged by size:
1393 1124 881 585 93 36
BmrI ACTGGGnnnn_n'
Cuts at: 130 823 1324 1405 1740 3884 130
Size: 693 501 81 335 2144 358
Fragments arranged by size:
2144 693 501 358 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 4112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 943 484
Size: 459 3653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 685 1288 2167 2881 3179 685
Size: 603 879 714 298 1618
Fragments arranged by size:
1618 879 714 603 298
BsaI GGTCTCn'nnnn_
Cuts at: 3816 3816
Size: 4112
BsaAI yAC'GTr
Cuts at: 486 1465 486
Size: 979 3133
BsaBI GATnn'nnATC
Cuts at: 6 1626 3663 6
Size: 1620 2037 455
Fragments arranged by size:
2037 1620 455
BsaHI Gr'CG_yC
Cuts at: 139 1067 1076 1088 1543 1791 2493 3989 139
Size: 928 9 12 455 248 702 1496 262
Fragments arranged by size:
1496 928 702 455 262 248 12 9
BsaWI w'CCGG_w
Cuts at: 1301 1822 2500 2911 3058 3332 1301
Size: 521 678 411 147 274 2081
Fragments arranged by size:
2081 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 175
Size: 4112
BseYI C'CCAG_C
Cuts at: 114 186 261 293 666 975 1029 1537 2295
Size: 72 75 32 373 309 54 508 758
Cuts at: 2295 2960 3368 114
Size: 665 408 858
Fragments arranged by size:
858 758 665 508 408 373 309 75
72 54 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 390
Size: 4112
BsiEI CG_ry'CG
Cuts at: 104 1101 1654 1700 2930 104
Size: 997 553 46 1230 1286
Fragments arranged by size:
1286 1230 997 553 46
BsiHKAI G_wGCw'C
Cuts at: 1126 1904 2094 2954 1126
Size: 778 190 860 2284
Fragments arranged by size:
2284 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 235 542 820 856 1126 1737 1830 1904 2094
Size: 307 278 36 270 611 93 74 190
Cuts at: 2094 2156 2954 235
Size: 62 798 1393
Fragments arranged by size:
1393 798 611 307 278 270 190 93
74 62 36
BspHI T'CATG_A
Cuts at: 2544 2544
Size: 4112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 926 1162 1376 1678 2059 926
Size: 236 214 302 381 2979
Fragments arranged by size:
2979 381 302 236 214
BsrBI CCG'CTC
Cuts at: 50 2404 2458 3942 4107 50
Size: 2354 54 1484 165 55
Fragments arranged by size:
2354 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2024 2024
Size: 4112
BsrFI r'CCGG_y
Cuts at: 674 2110 2291 674
Size: 1436 181 2495
Fragments arranged by size:
2495 1436 181
BssSI C'ACGA_G
Cuts at: 280 2383 3091 280
Size: 2103 708 1301
Fragments arranged by size:
2103 1301 708
BstAPI GCAn_nnn'nTGC
Cuts at: 663 1489 663
Size: 826 3286
BstBI TT'CG_AA
Cuts at: 2473 2473
Size: 4112
BstXI CCAn_nnnn'nTGG
Cuts at: 114 969 114
Size: 855 3257
BstYI r'GATC_y
Cuts at: 1 870 1137 1604 1627 1962 2208 2514 2526
Size: 869 267 467 23 335 246 306 12
Cuts at: 2526 2612 3664 1
Size: 86 1052 449
Fragments arranged by size:
1052 869 467 449 335 306 267 246
86 23 12
BtgI C'CryG_G
Cuts at: 177 303 860 965 177
Size: 126 557 105 3324
Fragments arranged by size:
3324 557 126 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2062 2243 2406 2062
Size: 181 163 3768
Fragments arranged by size:
3768 181 163
BtsI GCAGTG_nn'
Cuts at: 1355 1355
Size: 4112
DraI TTT'AAA
Cuts at: 1111 1111
Size: 4112
DraIII CAC_nnn'GTG
Cuts at: 426 426
Size: 4112
DrdI GACnn_nn'nnGTC
Cuts at: 1057 1093 1818 3162 1057
Size: 36 725 1344 2007
Fragments arranged by size:
2007 1344 725 36
EaeI y'GGCC_r
Cuts at: 318 789 822 959 1007 1098 1697 1871 2262
Size: 471 33 137 48 91 599 174 391
Cuts at: 2262 2289 3634 318
Size: 27 1345 796
Fragments arranged by size:
1345 796 599 471 391 174 137 91
48 33 27
EagI C'GGCC_G
Cuts at: 1098 1697 1098
Size: 599 3513
EarI CTCTTCn'nnn_
Cuts at: 884 2135 2345 884
Size: 1251 210 2651
Fragments arranged by size:
2651 1251 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 144 230 367 2320 3062 3208 3922 144
Size: 86 137 1953 742 146 714 334
Fragments arranged by size:
1953 742 714 334 146 137 86
EcoICRI GAG'CTC
Cuts at: 1124 1124
Size: 4112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 754 943 1937 2369 2722 3384 484
Size: 270 189 994 432 353 662 1212
Fragments arranged by size:
1212 994 662 432 353 270 189
EcoO109I rG'GnC_Cy
Cuts at: 205 231 232 830 852 853 1014 1251 205
Size: 26 1 598 22 1 161 237 3066
Fragments arranged by size:
3066 598 237 161 26 22 1 1
EcoRI G'AATT_C
Cuts at: 1116 1116
Size: 4112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 4112
FspI TGC'GCA
Cuts at: 1893 1893
Size: 4112
HaeII r_GCGC'y
Cuts at: 142 1079 1546 1794 3024 3795 142
Size: 937 467 248 1230 771 459
Fragments arranged by size:
1230 937 771 467 459 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 862 894 1954 1984 1986 2016 862
Size: 32 1060 30 2 30 2958
Fragments arranged by size:
2958 1060 32 30 30 2
HincII GTy'rAC
Cuts at: 1151 1151
Size: 4112
KasI G'GCGC_C
Cuts at: 138 1075 1542 1790 138
Size: 937 467 248 2460
Fragments arranged by size:
2460 937 467 248
KpnI G_GTAC'C
Cuts at: 1132 1132
Size: 4112
MluI A'CGCG_T
Cuts at: 3545 3545
Size: 4112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 503 2871 3055 503
Size: 2368 184 1560
Fragments arranged by size:
2368 1560 184
MscI TGG'CCA
Cuts at: 1873 1873
Size: 4112
MslI CAynn'nnrTG
Cuts at: 625 967 2101 2228 625
Size: 342 1134 127 2509
Fragments arranged by size:
2509 1134 342 127
MspA1I CmG'CkG
Cuts at: 114 297 784 1223 1537 1897 2681 2926 114
Size: 183 487 439 314 360 784 245 1300
Fragments arranged by size:
1300 784 487 439 360 314 245 183
NaeI GCC'GGC
Cuts at: 676 2293 676
Size: 1617 2495
NarI GG'CG_CC
Cuts at: 139 1076 1543 1791 139
Size: 937 467 248 2460
Fragments arranged by size:
2460 937 467 248
NcoI C'CATG_G
Cuts at: 303 965 303
Size: 662 3450
NgoMIV G'CCGG_C
Cuts at: 674 2291 674
Size: 1617 2495
NspI r_CATG'y
Cuts at: 1165 1665 3268 1165
Size: 500 1603 2009
Fragments arranged by size:
2009 1603 500
PciI A'CATG_T
Cuts at: 3264 3264
Size: 4112
PflMI CCAn_nnn'nTGG
Cuts at: 178 426 178
Size: 248 3864
PmeI GTTT'AAAC
Cuts at: 1111 1111
Size: 4112
PmlI CAC'GTG
Cuts at: 486 486
Size: 4112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 385 417 2558 2590 385
Size: 32 2141 32 1907
Fragments arranged by size:
2141 1907 32 32
PpuMI rG'GwC_Cy
Cuts at: 830 830
Size: 4112
PspOMI G'GGCC_C
Cuts at: 231 852 231
Size: 621 3491
PstI C_TGCA'G
Cuts at: 1159 1844 1159
Size: 685 3427
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4112
PvuII CAG'CTG
Cuts at: 297 1537 1897 297
Size: 1240 360 2512
Fragments arranged by size:
2512 1240 360
RsrII CG'GwC_CG
Cuts at: 2307 2307
Size: 4112
SacI G_AGCT'C
Cuts at: 1126 1126
Size: 4112
SalI G'TCGA_C
Cuts at: 1149 1149
Size: 4112
SanDI GG'GwC_CC
Cuts at: 830 830
Size: 4112
SapI GCTCTTCn'nnn_
Cuts at: 2135 2345 2135
Size: 210 3902
SbfI CC_TGCA'GG
Cuts at: 1159 1159
Size: 4112
ScaI AGT'ACT
Cuts at: 3629 3629
Size: 4112
SfcI C'TryA_G
Cuts at: 33 1155 1840 2808 2999 33
Size: 1122 685 968 191 1146
Fragments arranged by size:
1146 1122 968 685 191
SfoI GGC'GCC
Cuts at: 140 1077 1544 1792 140
Size: 937 467 248 2460
Fragments arranged by size:
2460 937 467 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4112
SmaI CCC'GGG
Cuts at: 850 1134 850
Size: 284 3828
SmlI C'TyrA_G
Cuts at: 700 1267 2182 2896 3158 700
Size: 567 915 714 262 1654
Fragments arranged by size:
1654 915 714 567 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4112
SphI G_CATG'C
Cuts at: 1165 1665 1165
Size: 500 3612
StuI AGG'CCT
Cuts at: 373 757 373
Size: 384 3728
StyI C'CwwG_G
Cuts at: 303 965 1091 1246 1600 303
Size: 662 126 155 354 2815
Fragments arranged by size:
2815 662 354 155 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 307 433 1021 1695 1828 2287 2496 307
Size: 126 588 674 133 459 209 1923
Fragments arranged by size:
1923 674 588 459 209 133 126
TatI w'GTAC_w
Cuts at: 3627 3627
Size: 4112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3629 3661 3629
Size: 32 4080
Tth111I GACn'n_nGTC
Cuts at: 1909 1909
Size: 4112
XbaI T'CTAG_A
Cuts at: 1143 1143
Size: 4112
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 301 906 972 1607 301
Size: 605 66 635 2806
Fragments arranged by size:
2806 635 605 66
XmaI C'CCGG_G
Cuts at: 848 1132 848
Size: 284 3828
ZraI GAC'GTC
Cuts at: 1068 1068
Size: 4112
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AlfI AloI AscI
AvrII BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PshAI PsiI PspXI PsrI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI