Restriction map for
pF1KB9475
(Circular) (Six-base) MAPSORT of: pF1KB9475.seq Check: 6303 from: 1 to: 4142
pF1KB9475 4142 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1180 1180
Size: 4142
Acc65I G'GTAC_C
Cuts at: 1158 1158
Size: 4142
AclI AA'CG_TT
Cuts at: 3982 3982
Size: 4142
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 248 782 835 872 893 909 1967 2399
Size: 34 534 53 37 21 16 1058 432
Cuts at: 2399 2752 3414 214
Size: 353 662 942
Fragments arranged by size:
1058 942 662 534 432 353 53 37
34 21 16
AflIII A'CryG_T
Cuts at: 988 1494 3294 3575 988
Size: 506 1800 281 1555
Fragments arranged by size:
1800 1555 506 281
AhdI GACnn_n'nnGTC
Cuts at: 903 903
Size: 4142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 988 988
Size: 4142
AlwNI CAG_nnn'CTG
Cuts at: 183 813 1447 2885 183
Size: 630 634 1438 1440
Fragments arranged by size:
1440 1438 634 630
ApaLI G'TGCA_C
Cuts at: 2980 2980
Size: 4142
ApoI r'AATT_y
Cuts at: 1132 1146 3495 3751 1132
Size: 14 2349 256 1523
Fragments arranged by size:
2349 1523 256 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 4142
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
AvaI C'yCGr_G
Cuts at: 1162 1162
Size: 4142
BamHI G'GATC_C
Cuts at: 1167 1167
Size: 4142
BanI G'GyrC_C
Cuts at: 930 1158 1572 1820 1855 930
Size: 228 414 248 35 3217
Fragments arranged by size:
3217 414 248 228 35
BanII G_rGCy'C
Cuts at: 190 1156 2186 190
Size: 966 1030 2146
Fragments arranged by size:
2146 1030 966
BbeI G_GCGC'C
Cuts at: 1576 1824 1576
Size: 248 3894
BbsI GAAGACnn'nnnn_
Cuts at: 142 191 899 142
Size: 49 708 3385
Fragments arranged by size:
3385 708 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4108
BciVI GTATCCnnnnn_n'
Cuts at: 516 2040 3096 4142 516
Size: 1524 1056 1046 516
Fragments arranged by size:
1524 1056 1046 516
BclI T'GATC_A
Cuts at: 1662 1662
Size: 4142
BglII A'GATC_T
Cuts at: 123 1657 3694 123
Size: 1534 2037 571
Fragments arranged by size:
2037 1534 571
BlpI GC'TnA_GC
Cuts at: 1254 1254
Size: 4142
Bme1580I G_kGCm'C
Cuts at: 1767 1860 2984 1767
Size: 93 1124 2925
Fragments arranged by size:
2925 1124 93
BmgBI CAC'GTC
Cuts at: 991 991
Size: 4142
BmrI ACTGGGnnnn_n'
Cuts at: 149 1354 1435 1770 3914 149
Size: 1205 81 335 2144 377
Fragments arranged by size:
2144 1205 377 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 255 2509 255
Size: 2254 1888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 371 730 371
Size: 359 3783
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1318 2197 2911 3209 1318
Size: 879 714 298 2251
Fragments arranged by size:
2251 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 331 3846 331
Size: 3515 627
BsaAI yAC'GTr
Cuts at: 1495 1495
Size: 4142
BsaBI GATnn'nnATC
Cuts at: 6 1656 3693 6
Size: 1650 2037 455
Fragments arranged by size:
2037 1650 455
BsaHI Gr'CG_yC
Cuts at: 1573 1821 2523 4019 1573
Size: 248 702 1496 1696
Fragments arranged by size:
1696 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1331 1852 2530 2941 3088 3362 1331
Size: 521 678 411 147 274 2111
Fragments arranged by size:
2111 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 784 814 784
Size: 30 4112
BseYI C'CCAG_C
Cuts at: 341 1567 2325 2990 3398 341
Size: 1226 758 665 408 1085
Fragments arranged by size:
1226 1085 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1684 1730 2960 104
Size: 1580 46 1230 1286
Fragments arranged by size:
1580 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 881 1156 1934 2124 2984 881
Size: 275 778 190 860 2039
Fragments arranged by size:
2039 860 778 275 190
Bsp1286I G_dGCh'C
Cuts at: 190 881 1156 1767 1860 1934 2124 2186 2984
Size: 691 275 611 93 74 190 62 798
Cuts at: 2984 190
Size: 1348
Fragments arranged by size:
1348 798 691 611 275 190 93 74
62
BspHI T'CATG_A
Cuts at: 2574 2574
Size: 4142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 255 1192 1406 1708 2089 255
Size: 937 214 302 381 2308
Fragments arranged by size:
2308 937 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2434 2488 3972 4137 50
Size: 2384 54 1484 165 55
Fragments arranged by size:
2384 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 576 2054 576
Size: 1478 2664
BsrFI r'CCGG_y
Cuts at: 2140 2321 2140
Size: 181 3961
BssSI C'ACGA_G
Cuts at: 2413 3121 2413
Size: 708 3434
BstAPI GCAn_nnn'nTGC
Cuts at: 1519 1519
Size: 4142
BstBI TT'CG_AA
Cuts at: 2503 2503
Size: 4142
BstYI r'GATC_y
Cuts at: 1 123 1167 1634 1657 1992 2238 2544 2556
Size: 122 1044 467 23 335 246 306 12
Cuts at: 2556 2642 3694 1
Size: 86 1052 449
Fragments arranged by size:
1052 1044 467 449 335 306 246 122
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2092 2273 2436 2092
Size: 181 163 3798
Fragments arranged by size:
3798 181 163
BtsI GCAGTG_nn'
Cuts at: 1385 1385
Size: 4142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 528 563 528
Size: 35 4107
DraI TTT'AAA
Cuts at: 1065 1141 1065
Size: 76 4066
DraIII CAC_nnn'GTG
Cuts at: 259 994 259
Size: 735 3407
DrdI GACnn_nn'nnGTC
Cuts at: 1848 3192 1848
Size: 1344 2798
EaeI y'GGCC_r
Cuts at: 136 1727 1901 2292 2319 3664 136
Size: 1591 174 391 27 1345 614
Fragments arranged by size:
1591 1345 614 391 174 27
EagI C'GGCC_G
Cuts at: 1727 1727
Size: 4142
EarI CTCTTCn'nnn_
Cuts at: 224 768 944 2165 2375 224
Size: 544 176 1221 210 1991
Fragments arranged by size:
1991 1221 544 210 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2350 3092 3238 3952 2350
Size: 742 146 714 2540
Fragments arranged by size:
2540 742 714 146
EcoICRI GAG'CTC
Cuts at: 1154 1154
Size: 4142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 248 371 730 782 835 872 893 909
Size: 34 123 359 52 53 37 21 16
Cuts at: 909 1967 2399 2752 3414 214
Size: 1058 432 353 662 942
Fragments arranged by size:
1058 942 662 432 359 353 123 53
52 37 34 21 16
EcoNI CCTnn'n_nnAGG
Cuts at: 938 938
Size: 4142
EcoO109I rG'GnC_Cy
Cuts at: 1281 1281
Size: 4142
EcoRI G'AATT_C
Cuts at: 1146 1146
Size: 4142
EcoRV GAT'ATC
Cuts at: 402 402
Size: 4142
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 303 2161 303
Size: 1858 2284
FspI TGC'GCA
Cuts at: 1923 1923
Size: 4142
HaeII r_GCGC'y
Cuts at: 1576 1824 3054 3825 1576
Size: 248 1230 771 1893
Fragments arranged by size:
1893 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 889 921 1984 2014 2016 2046 889
Size: 32 1063 30 2 30 2985
Fragments arranged by size:
2985 1063 32 30 30 2
HincII GTy'rAC
Cuts at: 1181 1181
Size: 4142
HindIII A'AGCT_T
Cuts at: 285 285
Size: 4142
KasI G'GCGC_C
Cuts at: 1572 1820 1572
Size: 248 3894
KpnI G_GTAC'C
Cuts at: 1162 1162
Size: 4142
MluI A'CGCG_T
Cuts at: 3575 3575
Size: 4142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1075 2901 3085 1075
Size: 1826 184 2132
Fragments arranged by size:
2132 1826 184
MscI TGG'CCA
Cuts at: 138 1903 138
Size: 1765 2377
MslI CAynn'nnrTG
Cuts at: 2131 2258 2131
Size: 127 4015
MspA1I CmG'CkG
Cuts at: 646 1253 1567 1927 2711 2956 646
Size: 607 314 360 784 245 1832
Fragments arranged by size:
1832 784 607 360 314 245
NaeI GCC'GGC
Cuts at: 2323 2323
Size: 4142
NarI GG'CG_CC
Cuts at: 1573 1821 1573
Size: 248 3894
NgoMIV G'CCGG_C
Cuts at: 2321 2321
Size: 4142
NspI r_CATG'y
Cuts at: 559 782 1195 1695 3298 559
Size: 223 413 500 1603 1403
Fragments arranged by size:
1603 1403 500 413 223
PciI A'CATG_T
Cuts at: 3294 3294
Size: 4142
PmeI GTTT'AAAC
Cuts at: 1141 1141
Size: 4142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2588 2620 2588
Size: 32 4110
PshAI GACnn'nnGTC
Cuts at: 740 740
Size: 4142
PstI C_TGCA'G
Cuts at: 315 964 1189 1874 315
Size: 649 225 685 2583
Fragments arranged by size:
2583 685 649 225
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4142
PvuII CAG'CTG
Cuts at: 646 1567 1927 646
Size: 921 360 2861
Fragments arranged by size:
2861 921 360
RsrII CG'GwC_CG
Cuts at: 2337 2337
Size: 4142
SacI G_AGCT'C
Cuts at: 1156 1156
Size: 4142
SalI G'TCGA_C
Cuts at: 1179 1179
Size: 4142
SapI GCTCTTCn'nnn_
Cuts at: 768 2165 2375 768
Size: 1397 210 2535
Fragments arranged by size:
2535 1397 210
SbfI CC_TGCA'GG
Cuts at: 1189 1189
Size: 4142
ScaI AGT'ACT
Cuts at: 3659 3659
Size: 4142
SexAI A'CCwGG_T
Cuts at: 500 500
Size: 4142
SfcI C'TryA_G
Cuts at: 33 311 960 1185 1870 2838 3029 33
Size: 278 649 225 685 968 191 1146
Fragments arranged by size:
1146 968 685 649 278 225 191
SfoI GGC'GCC
Cuts at: 1574 1822 1574
Size: 248 3894
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4142
SmaI CCC'GGG
Cuts at: 1164 1164
Size: 4142
SmlI C'TyrA_G
Cuts at: 1297 2212 2926 3188 1297
Size: 915 714 262 2251
Fragments arranged by size:
2251 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4142
SphI G_CATG'C
Cuts at: 1195 1695 1195
Size: 500 3642
StyI C'CwwG_G
Cuts at: 204 1276 1630 204
Size: 1072 354 2716
Fragments arranged by size:
2716 1072 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 552 1725 1858 2317 2526 552
Size: 1173 133 459 209 2168
Fragments arranged by size:
2168 1173 459 209 133
TatI w'GTAC_w
Cuts at: 563 3657 563
Size: 3094 1048
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 315 347 784 816 3659 3691 315
Size: 32 437 32 2843 32 766
Fragments arranged by size:
2843 766 437 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1939 1939
Size: 4142
XbaI T'CTAG_A
Cuts at: 660 1173 660
Size: 513 3629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1637 1637
Size: 4142
XmaI C'CCGG_G
Cuts at: 1162 1162
Size: 4142
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I
BseRI BsgI BsiWI BsmI BsmBI BspEI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI