Restriction map for
pF1KB9473
(Circular) (Six-base) MAPSORT of: pF1KB9473.seq Check: 5029 from: 1 to: 5609
pF1KB9473 5609 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 260 2647 260
Size: 2387 3222
Acc65I G'GTAC_C
Cuts at: 2625 2625
Size: 5609
AclI AA'CG_TT
Cuts at: 5449 5449
Size: 5609
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 926 1888 3434 3866 4219 4881 625
Size: 301 962 1546 432 353 662 1353
Fragments arranged by size:
1546 1353 962 662 432 353 301
AflIII A'CryG_T
Cuts at: 2961 4761 5042 2961
Size: 1800 281 3528
Fragments arranged by size:
3528 1800 281
AleI CACnn'nnGTG
Cuts at: 2374 2374
Size: 5609
AlwNI CAG_nnn'CTG
Cuts at: 537 2423 2914 4352 537
Size: 1886 491 1438 1794
Fragments arranged by size:
1886 1794 1438 491
ApaLI G'TGCA_C
Cuts at: 4447 4447
Size: 5609
ApoI r'AATT_y
Cuts at: 802 1433 2096 2613 4962 5218 802
Size: 631 663 517 2349 256 1193
Fragments arranged by size:
2349 1193 663 631 517 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5609
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5609
AvaI C'yCGr_G
Cuts at: 172 2447 2629 172
Size: 2275 182 3152
Fragments arranged by size:
3152 2275 182
BamHI G'GATC_C
Cuts at: 2634 2634
Size: 5609
BanI G'GyrC_C
Cuts at: 176 207 241 564 2625 3039 3287 3322 176
Size: 31 34 323 2061 414 248 35 2463
Fragments arranged by size:
2463 2061 414 323 248 35 34 31
BanII G_rGCy'C
Cuts at: 1683 2623 3653 1683
Size: 940 1030 3639
Fragments arranged by size:
3639 1030 940
BbeI G_GCGC'C
Cuts at: 245 3043 3291 245
Size: 2798 248 2563
Fragments arranged by size:
2798 2563 248
BbsI GAAGACnn'nnnn_
Cuts at: 1041 2592 1041
Size: 1551 4058
BbvCI CC'TCA_GC
Cuts at: 280 2035 280
Size: 1755 3854
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5575
BciVI GTATCCnnnnn_n'
Cuts at: 2146 3507 4563 5609 2146
Size: 1361 1056 1046 2146
Fragments arranged by size:
2146 1361 1056 1046
BclI T'GATC_A
Cuts at: 3129 3129
Size: 5609
BglII A'GATC_T
Cuts at: 3124 5161 3124
Size: 2037 3572
BlpI GC'TnA_GC
Cuts at: 2721 2721
Size: 5609
Bme1580I G_kGCm'C
Cuts at: 179 775 3234 3327 4451 179
Size: 596 2459 93 1124 1337
Fragments arranged by size:
2459 1337 1124 596 93
BmgBI CAC'GTC
Cuts at: 696 696
Size: 5609
BmrI ACTGGGnnnn_n'
Cuts at: 2338 2821 2902 3237 5381 2338
Size: 483 81 335 2144 2566
Fragments arranged by size:
2566 2144 483 335 81
BmtI G_CTAG'C
Cuts at: 2032 2032
Size: 5609
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1251 3976 1251
Size: 2725 2884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1221 1221
Size: 5609
Bpu10I CC'TnA_GC
Cuts at: 280 2035 280
Size: 1755 3854
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2785 3664 4378 4676 2785
Size: 879 714 298 3718
Fragments arranged by size:
3718 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1741 2579 5313 1741
Size: 838 2734 2037
Fragments arranged by size:
2734 2037 838
BsaAI yAC'GTr
Cuts at: 1620 2962 1620
Size: 1342 4267
BsaBI GATnn'nnATC
Cuts at: 6 251 3123 5160 6
Size: 245 2872 2037 455
Fragments arranged by size:
2872 2037 455 245
BsaHI Gr'CG_yC
Cuts at: 166 242 1577 3040 3288 3990 5486 166
Size: 76 1335 1463 248 702 1496 289
Fragments arranged by size:
1496 1463 1335 702 289 248 76
BsaWI w'CCGG_w
Cuts at: 620 1361 1406 2798 3319 3997 4408 4555 4829
Size: 741 45 1392 521 678 411 147 274
Cuts at: 4829 620
Size: 1400
Fragments arranged by size:
1400 1392 741 678 521 411 274 147
45
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 474 1251 1324 474
Size: 777 73 4759
Fragments arranged by size:
4759 777 73
BseYI C'CCAG_C
Cuts at: 2262 2423 3034 3792 4457 4865 2262
Size: 161 611 758 665 408 3006
Fragments arranged by size:
3006 758 665 611 408 161
BsiEI CG_ry'CG
Cuts at: 104 2441 3151 3197 4427 104
Size: 2337 710 46 1230 1286
Fragments arranged by size:
2337 1286 1230 710 46
BsiHKAI G_wGCw'C
Cuts at: 579 1566 1781 2623 3401 3591 4451 579
Size: 987 215 842 778 190 860 1737
Fragments arranged by size:
1737 987 860 842 778 215 190
BsmI GAATG_Cn'
Cuts at: 2468 2468
Size: 5609
BsmBI CGTCTCn'nnnn_
Cuts at: 511 511
Size: 5609
Bsp1286I G_dGCh'C
Cuts at: 179 579 775 1566 1683 1781 2623 3234 3327
Size: 400 196 791 117 98 842 611 93
Cuts at: 3327 3401 3591 3653 4451 179
Size: 74 190 62 798 1337
Fragments arranged by size:
1337 842 798 791 611 400 196 190
117 98 93 74 62
BspEI T'CCGG_A
Cuts at: 620 1361 620
Size: 741 4868
BspHI T'CATG_A
Cuts at: 2312 4041 2312
Size: 1729 3880
BspMI ACCTGCnnnn'nnnn_
Cuts at: 417 2659 2873 3175 3556 417
Size: 2242 214 302 381 2470
Fragments arranged by size:
2470 2242 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2352 3901 3955 5439 5604 50
Size: 2302 1549 54 1484 165 55
Fragments arranged by size:
2302 1549 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1181 1457 1754 3521 1181
Size: 276 297 1767 3269
Fragments arranged by size:
3269 1767 297 276
BsrFI r'CCGG_y
Cuts at: 3607 3788 3607
Size: 181 5428
BsrGI T'GTAC_A
Cuts at: 1841 1841
Size: 5609
BssHII G'CGCG_C
Cuts at: 140 140
Size: 5609
BssSI C'ACGA_G
Cuts at: 3880 4588 3880
Size: 708 4901
BstAPI GCAn_nnn'nTGC
Cuts at: 2986 2986
Size: 5609
BstBI TT'CG_AA
Cuts at: 3970 3970
Size: 5609
BstEII G'GTnAC_C
Cuts at: 1947 1947
Size: 5609
BstXI CCAn_nnnn'nTGG
Cuts at: 696 696
Size: 5609
BstYI r'GATC_y
Cuts at: 1 925 2591 2634 3101 3124 3459 3705 4011
Size: 924 1666 43 467 23 335 246 306
Cuts at: 4011 4023 4109 5161 1
Size: 12 86 1052 449
Fragments arranged by size:
1666 1052 924 467 449 335 306 246
86 43 23 12
BtgI C'CryG_G
Cuts at: 560 1304 2114 560
Size: 744 810 4055
Fragments arranged by size:
4055 810 744
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3559 3740 3903 3559
Size: 181 163 5265
Fragments arranged by size:
5265 181 163
BtsI GCAGTG_nn'
Cuts at: 2149 2852 2149
Size: 703 4906
DraI TTT'AAA
Cuts at: 2608 2608
Size: 5609
DraIII CAC_nnn'GTG
Cuts at: 798 798
Size: 5609
DrdI GACnn_nn'nnGTC
Cuts at: 3315 4659 3315
Size: 1344 4265
EaeI y'GGCC_r
Cuts at: 2366 2438 3194 3368 3759 3786 5131 2366
Size: 72 756 174 391 27 1345 2844
Fragments arranged by size:
2844 1345 756 391 174 72 27
EagI C'GGCC_G
Cuts at: 2438 3194 2438
Size: 756 4853
EarI CTCTTCn'nnn_
Cuts at: 445 1642 1744 2359 3632 3842 445
Size: 1197 102 615 1273 210 2212
Fragments arranged by size:
2212 1273 1197 615 210 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3817 4559 4705 5419 3817
Size: 742 146 714 4007
Fragments arranged by size:
4007 742 714 146
EcoICRI GAG'CTC
Cuts at: 2621 2621
Size: 5609
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 926 1221 1888 3434 3866 4219 4881 625
Size: 301 295 667 1546 432 353 662 1353
Fragments arranged by size:
1546 1353 667 662 432 353 301 295
EcoO109I rG'GnC_Cy
Cuts at: 523 2337 2748 523
Size: 1814 411 3384
Fragments arranged by size:
3384 1814 411
EcoRI G'AATT_C
Cuts at: 2613 2613
Size: 5609
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1716 3628 1716
Size: 1912 3697
FspI TGC'GCA
Cuts at: 3390 3390
Size: 5609
HaeII r_GCGC'y
Cuts at: 245 1910 3043 3291 4521 5292 245
Size: 1665 1133 248 1230 771 562
Fragments arranged by size:
1665 1230 1133 771 562 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 403 435 541 573 685 717 948 980 2576
Size: 32 106 32 112 32 231 32 1596
Cuts at: 2576 2608 3451 3481 3483 3513 403
Size: 32 843 30 2 30 2499
Fragments arranged by size:
2499 1596 843 231 112 106 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2202 2552 2648 2202
Size: 350 96 5163
Fragments arranged by size:
5163 350 96
HindIII A'AGCT_T
Cuts at: 1134 1134
Size: 5609
HpaI GTT'AAC
Cuts at: 2202 2552 2202
Size: 350 5259
KasI G'GCGC_C
Cuts at: 241 3039 3287 241
Size: 2798 248 2563
Fragments arranged by size:
2798 2563 248
KpnI G_GTAC'C
Cuts at: 2629 2629
Size: 5609
MluI A'CGCG_T
Cuts at: 5042 5042
Size: 5609
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1838 4368 4552 1838
Size: 2530 184 2895
Fragments arranged by size:
2895 2530 184
MscI TGG'CCA
Cuts at: 2368 3370 2368
Size: 1002 4607
MslI CAynn'nnrTG
Cuts at: 715 1462 1843 2311 2374 3598 3725 715
Size: 747 381 468 63 1224 127 2599
Fragments arranged by size:
2599 1224 747 468 381 127 63
MspA1I CmG'CkG
Cuts at: 266 1491 2374 2423 2720 3034 3394 4178 4423
Size: 1225 883 49 297 314 360 784 245
Cuts at: 4423 266
Size: 1452
Fragments arranged by size:
1452 1225 883 784 360 314 297 245
49
NaeI GCC'GGC
Cuts at: 3790 3790
Size: 5609
NarI GG'CG_CC
Cuts at: 242 3040 3288 242
Size: 2798 248 2563
Fragments arranged by size:
2798 2563 248
NcoI C'CATG_G
Cuts at: 560 2114 560
Size: 1554 4055
NgoMIV G'CCGG_C
Cuts at: 3788 3788
Size: 5609
NheI G'CTAG_C
Cuts at: 2028 2028
Size: 5609
NspI r_CATG'y
Cuts at: 1866 2262 2662 3162 4765 1866
Size: 396 400 500 1603 2710
Fragments arranged by size:
2710 1603 500 400 396
PasI CC'CwG_GG
Cuts at: 570 2240 570
Size: 1670 3939
PciI A'CATG_T
Cuts at: 4761 4761
Size: 5609
PflMI CCAn_nnn'nTGG
Cuts at: 798 2019 798
Size: 1221 4388
PfoI T'CCnGG_A
Cuts at: 938 1242 1396 938
Size: 304 154 5151
Fragments arranged by size:
5151 304 154
PmeI GTTT'AAAC
Cuts at: 2608 2608
Size: 5609
PmlI CAC'GTG
Cuts at: 1620 1620
Size: 5609
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 283 315 346 378 4055 4087 283
Size: 32 31 32 3677 32 1805
Fragments arranged by size:
3677 1805 32 32 32 31
PpuMI rG'GwC_Cy
Cuts at: 523 2337 523
Size: 1814 3795
PshAI GACnn'nnGTC
Cuts at: 997 997
Size: 5609
PstI C_TGCA'G
Cuts at: 2656 3341 2656
Size: 685 4924
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5609
PvuII CAG'CTG
Cuts at: 1491 2423 3034 3394 1491
Size: 932 611 360 3706
Fragments arranged by size:
3706 932 611 360
RsrII CG'GwC_CG
Cuts at: 3804 3804
Size: 5609
SacI G_AGCT'C
Cuts at: 2623 2623
Size: 5609
SalI G'TCGA_C
Cuts at: 2646 2646
Size: 5609
SanDI GG'GwC_CC
Cuts at: 523 523
Size: 5609
SapI GCTCTTCn'nnn_
Cuts at: 445 2359 3632 3842 445
Size: 1914 1273 210 2212
Fragments arranged by size:
2212 1914 1273 210
SbfI CC_TGCA'GG
Cuts at: 2656 2656
Size: 5609
ScaI AGT'ACT
Cuts at: 5126 5126
Size: 5609
SexAI A'CCwGG_T
Cuts at: 326 326
Size: 5609
SfcI C'TryA_G
Cuts at: 33 261 850 967 2652 3337 4305 4496 33
Size: 228 589 117 1685 685 968 191 1146
Fragments arranged by size:
1685 1146 968 685 589 228 191 117
SfoI GGC'GCC
Cuts at: 243 3041 3289 243
Size: 2798 248 2563
Fragments arranged by size:
2798 2563 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5609
SmaI CCC'GGG
Cuts at: 174 2449 2631 174
Size: 2275 182 3152
Fragments arranged by size:
3152 2275 182
SmlI C'TyrA_G
Cuts at: 2764 3679 4393 4655 2764
Size: 915 714 262 3718
Fragments arranged by size:
3718 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5609
SphI G_CATG'C
Cuts at: 1866 2662 3162 1866
Size: 796 500 4313
Fragments arranged by size:
4313 796 500
SrfI GCCC'GGGC
Cuts at: 174 174
Size: 5609
StyI C'CwwG_G
Cuts at: 444 560 1571 1691 1956 2114 2743 3097 444
Size: 116 1011 120 265 158 629 354 2956
Fragments arranged by size:
2956 1011 629 354 265 158 120 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 562 1904 2056 3192 3325 3784 3993 562
Size: 1342 152 1136 133 459 209 2178
Fragments arranged by size:
2178 1342 1136 459 209 152 133
TatI w'GTAC_w
Cuts at: 581 1841 5124 581
Size: 1260 3283 1066
Fragments arranged by size:
3283 1260 1066
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 480 512 1151 1183 5126 5158 480
Size: 32 639 32 3943 32 931
Fragments arranged by size:
3943 931 639 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3406 3406
Size: 5609
XbaI T'CTAG_A
Cuts at: 2640 2640
Size: 5609
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 567 3104 567
Size: 2537 3072
XmaI C'CCGG_G
Cuts at: 172 2447 2629 172
Size: 2275 182 3152
Fragments arranged by size:
3152 2275 182
XmnI GAAnn'nnTTC
Cuts at: 610 610
Size: 5609
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BfrBI BglI BsaXI BsgI
BsiWI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI MfeI NdeI NotI NruI NsiI PacI PsiI
PspOMI PspXI PsrI SacII SfiI SgrAI SnaBI SspI
StuI SwaI XhoI ZraI