Restriction map for
pF1KB9470
(Circular) (Six-base) MAPSORT of: pF1KB9470.seq Check: 3447 from: 1 to: 5078
pF1KB9470 5078 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 2116 2116
Size: 5078
Acc65I G'GTAC_C
Cuts at: 2094 2094
Size: 5078
AclI AA'CG_TT
Cuts at: 4918 4918
Size: 5078
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 259 691 2903 3335 3688 4350 244
Size: 15 432 2212 432 353 662 972
Fragments arranged by size:
2212 972 662 432 432 353 15
AfeI AGC'GCT
Cuts at: 277 277
Size: 5078
AflIII A'CryG_T
Cuts at: 2430 4230 4511 2430
Size: 1800 281 2997
Fragments arranged by size:
2997 1800 281
AgeI A'CCGG_T
Cuts at: 1032 1032
Size: 5078
AhdI GACnn_n'nnGTC
Cuts at: 1662 1662
Size: 5078
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1621 1621
Size: 5078
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 541 573 1357 1389 541
Size: 32 784 32 4230
Fragments arranged by size:
4230 784 32 32
AlwNI CAG_nnn'CTG
Cuts at: 900 987 1251 1562 1782 2383 3821 900
Size: 87 264 311 220 601 1438 2157
Fragments arranged by size:
2157 1438 601 311 264 220 87
ApaI G_GGCC'C
Cuts at: 144 217 1049 1623 2067 144
Size: 73 832 574 444 3155
Fragments arranged by size:
3155 832 574 444 73
ApaLI G'TGCA_C
Cuts at: 649 3916 649
Size: 3267 1811
ApoI r'AATT_y
Cuts at: 2082 4431 4687 2082
Size: 2349 256 2473
Fragments arranged by size:
2473 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5078
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5078
AvaI C'yCGr_G
Cuts at: 391 694 1425 1745 2098 391
Size: 303 731 320 353 3371
Fragments arranged by size:
3371 731 353 320 303
AvrII C'CTAG_G
Cuts at: 2028 2028
Size: 5078
BamHI G'GATC_C
Cuts at: 2103 2103
Size: 5078
BanI G'GyrC_C
Cuts at: 492 1029 1329 1705 2056 2094 2508 2756 2791
Size: 537 300 376 351 38 414 248 35
Cuts at: 2791 492
Size: 2779
Fragments arranged by size:
2779 537 414 376 351 300 248 38
35
BanII G_rGCy'C
Cuts at: 118 144 184 217 542 692 701 1049 1093
Size: 26 40 33 325 150 9 348 44
Cuts at: 1093 1127 1432 1552 1623 1631 1724 1796 2067
Size: 34 305 120 71 8 93 72 271
Cuts at: 2067 2092 3122 118
Size: 25 1030 2074
Fragments arranged by size:
2074 1030 348 325 305 271 150 120
93 72 71 44 40 34 33 26
25 9 8
BbeI G_GCGC'C
Cuts at: 496 2512 2760 496
Size: 2016 248 2814
Fragments arranged by size:
2814 2016 248
BbvCI CC'TCA_GC
Cuts at: 733 733
Size: 5078
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 957 991 1573 1607 90
Size: 34 833 34 582 34 3561
Fragments arranged by size:
3561 833 582 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2976 4032 5078 2976
Size: 1056 1046 2976
Fragments arranged by size:
2976 1056 1046
BclI T'GATC_A
Cuts at: 2598 2598
Size: 5078
BglI GCCn_nnn'nGGC
Cuts at: 139 1507 139
Size: 1368 3710
BglII A'GATC_T
Cuts at: 2593 4630 2593
Size: 2037 3041
BlpI GC'TnA_GC
Cuts at: 112 2190 112
Size: 2078 3000
Bme1580I G_kGCm'C
Cuts at: 144 202 217 653 775 1049 1334 1466 1623
Size: 58 15 436 122 274 285 132 157
Cuts at: 1623 2067 2703 2796 3920 144
Size: 444 636 93 1124 1302
Fragments arranged by size:
1302 1124 636 444 436 285 274 157
132 122 93 58 15
BmgBI CAC'GTC
Cuts at: 648 648
Size: 5078
BmrI ACTGGGnnnn_n'
Cuts at: 943 1250 1626 2290 2371 2706 4850 943
Size: 307 376 664 81 335 2144 1171
Fragments arranged by size:
2144 1171 664 376 335 307 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1432 3445 1432
Size: 2013 3065
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 799 1176 1345 1645 799
Size: 377 169 300 4232
Fragments arranged by size:
4232 377 300 169
Bpu10I CC'TnA_GC
Cuts at: 733 1795 733
Size: 1062 4016
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1402 2254 3133 3847 4145 1402
Size: 852 879 714 298 2335
Fragments arranged by size:
2335 879 852 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1403 4782 1403
Size: 3379 1699
BsaAI yAC'GTr
Cuts at: 2431 2431
Size: 5078
BsaBI GATnn'nnATC
Cuts at: 6 2592 4629 6
Size: 2586 2037 455
Fragments arranged by size:
2586 2037 455
BsaHI Gr'CG_yC
Cuts at: 493 2509 2757 3459 4955 493
Size: 2016 248 702 1496 616
Fragments arranged by size:
2016 1496 702 616 248
BsaWI w'CCGG_w
Cuts at: 1032 2267 2788 3466 3877 4024 4298 1032
Size: 1235 521 678 411 147 274 1812
Fragments arranged by size:
1812 1235 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 541 571 1414 1444 541
Size: 30 843 30 4175
Fragments arranged by size:
4175 843 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 264 562 1111 1332 1848 1995 2026 264
Size: 298 549 221 516 147 31 3316
Fragments arranged by size:
3316 549 516 298 221 147 31
BseYI C'CCAG_C
Cuts at: 210 743 1492 1642 1723 2503 3261 3926 4334
Size: 533 749 150 81 780 758 665 408
Cuts at: 4334 210
Size: 954
Fragments arranged by size:
954 780 758 749 665 533 408 150
81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1245 1264 1456 1548 1591 1245
Size: 19 192 92 43 4732
Fragments arranged by size:
4732 192 92 43 19
BsiEI CG_ry'CG
Cuts at: 104 1178 2620 2666 3896 104
Size: 1074 1442 46 1230 1286
Fragments arranged by size:
1442 1286 1230 1074 46
BsiHKAI G_wGCw'C
Cuts at: 653 692 1190 1796 2092 2870 3060 3920 653
Size: 39 498 606 296 778 190 860 1811
Fragments arranged by size:
1811 860 778 606 498 296 190 39
Bsp1286I G_dGCh'C
Cuts at: 118 144 184 202 217 542 653 692 701
Size: 26 40 18 15 325 111 39 9
Cuts at: 701 775 1049 1093 1127 1190 1334 1432 1466
Size: 74 274 44 34 63 144 98 34
Cuts at: 1466 1552 1623 1631 1724 1796 2067 2092 2703
Size: 86 71 8 93 72 271 25 611
Cuts at: 2703 2796 2870 3060 3122 3920 118
Size: 93 74 190 62 798 1276
Fragments arranged by size:
1276 798 611 325 274 271 190 144
111 98 93 93 86 74 74 72
71 63 62 44 40 39 34 34
26 25 18 15 9 8
BspHI T'CATG_A
Cuts at: 257 3510 257
Size: 3253 1825
BspMI ACCTGCnnnn'nnnn_
Cuts at: 602 689 833 2128 2342 2644 3025 602
Size: 87 144 1295 214 302 381 2655
Fragments arranged by size:
2655 1295 381 302 214 144 87
BsrBI CCG'CTC
Cuts at: 50 146 471 1638 1763 3370 3424 4908 5073
Size: 96 325 1167 125 1607 54 1484 165
Cuts at: 5073 50
Size: 55
Fragments arranged by size:
1607 1484 1167 325 165 125 96 55
54
BsrDI GCAATG_nn'
Cuts at: 1078 2990 1078
Size: 1912 3166
BsrFI r'CCGG_y
Cuts at: 1032 1220 1412 3076 3257 1032
Size: 188 192 1664 181 2853
Fragments arranged by size:
2853 1664 192 188 181
BssHII G'CGCG_C
Cuts at: 1756 1756
Size: 5078
BssSI C'ACGA_G
Cuts at: 466 3349 4057 466
Size: 2883 708 1487
Fragments arranged by size:
2883 1487 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1251 1571 2056 2455 1251
Size: 320 485 399 3874
Fragments arranged by size:
3874 485 399 320
BstBI TT'CG_AA
Cuts at: 3439 3439
Size: 5078
BstEII G'GTnAC_C
Cuts at: 1101 1101
Size: 5078
BstXI CCAn_nnnn'nTGG
Cuts at: 1668 1668
Size: 5078
BstYI r'GATC_y
Cuts at: 1 431 2103 2570 2593 2928 3174 3480 3492
Size: 430 1672 467 23 335 246 306 12
Cuts at: 3492 3578 4630 1
Size: 86 1052 449
Fragments arranged by size:
1672 1052 467 449 430 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 809 809
Size: 5078
BtgI C'CryG_G
Cuts at: 701 1178 1205 701
Size: 477 27 4574
Fragments arranged by size:
4574 477 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3028 3209 3372 3028
Size: 181 163 4734
Fragments arranged by size:
4734 181 163
BtsI GCAGTG_nn'
Cuts at: 517 1085 2321 517
Size: 568 1236 3274
Fragments arranged by size:
3274 1236 568
DraI TTT'AAA
Cuts at: 2077 2077
Size: 5078
DraIII CAC_nnn'GTG
Cuts at: 657 657
Size: 5078
DrdI GACnn_nn'nnGTC
Cuts at: 2784 4128 2784
Size: 1344 3734
EaeI y'GGCC_r
Cuts at: 931 1175 1499 2663 2837 3228 3255 4600 931
Size: 244 324 1164 174 391 27 1345 1409
Fragments arranged by size:
1409 1345 1164 391 324 244 174 27
EagI C'GGCC_G
Cuts at: 1175 2663 1175
Size: 1488 3590
EarI CTCTTCn'nnn_
Cuts at: 1027 1164 3101 3311 1027
Size: 137 1937 210 2794
Fragments arranged by size:
2794 1937 210 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 204 3286 4028 4174 4888 204
Size: 3082 742 146 714 394
Fragments arranged by size:
3082 742 714 394 146
EcoICRI GAG'CTC
Cuts at: 690 1794 2090 690
Size: 1104 296 3678
Fragments arranged by size:
3678 1104 296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 244 259 691 799 1176 1345 1645 2903 3335
Size: 15 432 108 377 169 300 1258 432
Cuts at: 3335 3688 4350 244
Size: 353 662 972
Fragments arranged by size:
1258 972 662 432 432 377 353 300
169 108 15
EcoNI CCTnn'n_nnAGG
Cuts at: 714 808 714
Size: 94 4984
EcoO109I rG'GnC_Cy
Cuts at: 140 214 396 591 945 1045 1065 1154 1286
Size: 74 182 195 354 100 20 89 132
Cuts at: 1286 1520 1620 1656 2063 2064 2217 140
Size: 234 100 36 407 1 153 3001
Fragments arranged by size:
3001 407 354 234 195 182 153 132
100 100 89 74 36 20 1
EcoRI G'AATT_C
Cuts at: 2082 2082
Size: 5078
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 429 1287 3097 429
Size: 858 1810 2410
Fragments arranged by size:
2410 1810 858
FspI TGC'GCA
Cuts at: 1077 2859 1077
Size: 1782 3296
HaeII r_GCGC'y
Cuts at: 279 447 496 2512 2760 3990 4761 279
Size: 168 49 2016 248 1230 771 596
Fragments arranged by size:
2016 1230 771 596 248 168 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1414 1446 1648 1680 2920 2950 2952 2982 1414
Size: 32 202 32 1240 30 2 30 3510
Fragments arranged by size:
3510 1240 202 32 32 30 30 2
HincII GTy'rAC
Cuts at: 879 2117 879
Size: 1238 3840
HindIII A'AGCT_T
Cuts at: 2034 2034
Size: 5078
KasI G'GCGC_C
Cuts at: 492 2508 2756 492
Size: 2016 248 2814
Fragments arranged by size:
2814 2016 248
KpnI G_GTAC'C
Cuts at: 2098 2098
Size: 5078
MluI A'CGCG_T
Cuts at: 4511 4511
Size: 5078
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1240 3837 4021 1240
Size: 2597 184 2297
Fragments arranged by size:
2597 2297 184
MscI TGG'CCA
Cuts at: 1501 2839 1501
Size: 1338 3740
MslI CAynn'nnrTG
Cuts at: 3067 3194 3067
Size: 127 4951
MspA1I CmG'CkG
Cuts at: 737 747 1562 1818 1992 2189 2503 2863 3647
Size: 10 815 256 174 197 314 360 784
Cuts at: 3647 3892 737
Size: 245 1923
Fragments arranged by size:
1923 815 784 360 314 256 245 197
174 10
NaeI GCC'GGC
Cuts at: 3259 3259
Size: 5078
NarI GG'CG_CC
Cuts at: 493 2509 2757 493
Size: 2016 248 2814
Fragments arranged by size:
2814 2016 248
NgoMIV G'CCGG_C
Cuts at: 3257 3257
Size: 5078
NspI r_CATG'y
Cuts at: 567 2131 2631 4234 567
Size: 1564 500 1603 1411
Fragments arranged by size:
1603 1564 1411 500
PasI CC'CwG_GG
Cuts at: 184 339 400 427 1231 184
Size: 155 61 27 804 4031
Fragments arranged by size:
4031 804 155 61 27
PciI A'CATG_T
Cuts at: 4230 4230
Size: 5078
PflMI CCAn_nnn'nTGG
Cuts at: 900 1686 900
Size: 786 4292
PfoI T'CCnGG_A
Cuts at: 527 1037 527
Size: 510 4568
PmeI GTTT'AAAC
Cuts at: 2077 2077
Size: 5078
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 541 573 3524 3556 541
Size: 32 2951 32 2063
Fragments arranged by size:
2951 2063 32 32
PpuMI rG'GwC_Cy
Cuts at: 396 591 945 1154 1286 1656 396
Size: 195 354 209 132 370 3818
Fragments arranged by size:
3818 370 354 209 195 132
PspOMI G'GGCC_C
Cuts at: 140 213 1045 1619 2063 140
Size: 73 832 574 444 3155
Fragments arranged by size:
3155 832 574 444 73
PstI C_TGCA'G
Cuts at: 599 2125 2810 599
Size: 1526 685 2867
Fragments arranged by size:
2867 1526 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5078
PvuII CAG'CTG
Cuts at: 737 747 1562 1992 2503 2863 737
Size: 10 815 430 511 360 2952
Fragments arranged by size:
2952 815 511 430 360 10
RsrII CG'GwC_CG
Cuts at: 3273 3273
Size: 5078
SacI G_AGCT'C
Cuts at: 692 1796 2092 692
Size: 1104 296 3678
Fragments arranged by size:
3678 1104 296
SalI G'TCGA_C
Cuts at: 2115 2115
Size: 5078
SapI GCTCTTCn'nnn_
Cuts at: 1027 3101 3311 1027
Size: 2074 210 2794
Fragments arranged by size:
2794 2074 210
SbfI CC_TGCA'GG
Cuts at: 599 2125 599
Size: 1526 3552
ScaI AGT'ACT
Cuts at: 4595 4595
Size: 5078
SexAI A'CCwGG_T
Cuts at: 581 581
Size: 5078
SfcI C'TryA_G
Cuts at: 33 595 2121 2806 3774 3965 33
Size: 562 1526 685 968 191 1146
Fragments arranged by size:
1526 1146 968 685 562 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 139 139
Size: 5078
SfoI GGC'GCC
Cuts at: 494 2510 2758 494
Size: 2016 248 2814
Fragments arranged by size:
2814 2016 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5078
SmaI CCC'GGG
Cuts at: 1747 2100 1747
Size: 353 4725
SmlI C'TyrA_G
Cuts at: 1417 2233 3148 3862 4124 1417
Size: 816 915 714 262 2371
Fragments arranged by size:
2371 915 816 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5078
SphI G_CATG'C
Cuts at: 567 2131 2631 567
Size: 1564 500 3014
Fragments arranged by size:
3014 1564 500
StuI AGG'CCT
Cuts at: 1771 1771
Size: 5078
StyI C'CwwG_G
Cuts at: 755 1116 2028 2212 2566 755
Size: 361 912 184 354 3267
Fragments arranged by size:
3267 912 361 354 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2661 2794 3253 3462 2661
Size: 133 459 209 4277
Fragments arranged by size:
4277 459 209 133
TatI w'GTAC_w
Cuts at: 4593 4593
Size: 5078
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4595 4627 4595
Size: 32 5046
Tth111I GACn'n_nGTC
Cuts at: 2875 2875
Size: 5078
XbaI T'CTAG_A
Cuts at: 2109 2109
Size: 5078
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2573 2573
Size: 5078
XmaI C'CCGG_G
Cuts at: 1745 2098 1745
Size: 353 4725
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AscI BaeI BbsI BfrBI
BmtI BsiWI BsmI BsmBI BspEI BsrGI BstZ17I ClaI
CspCI EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI