Restriction map for
pF1KB9469
(Circular) (Six-base) MAPSORT of: pF1KB9469.seq Check: 4897 from: 1 to: 5519
pF1KB9469 5519 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 2557 2557
Size: 5519
Acc65I G'GTAC_C
Cuts at: 2535 2535
Size: 5519
AclI AA'CG_TT
Cuts at: 5359 5359
Size: 5519
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1081 3344 3776 4129 4791 1081
Size: 2263 432 353 662 1809
Fragments arranged by size:
2263 1809 662 432 353
AfeI AGC'GCT
Cuts at: 252 1821 1888 252
Size: 1569 67 3883
Fragments arranged by size:
3883 1569 67
AflII C'TTAA_G
Cuts at: 853 853
Size: 5519
AflIII A'CryG_T
Cuts at: 2871 4671 4952 2871
Size: 1800 281 3438
Fragments arranged by size:
3438 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1627 1627
Size: 5519
AlwNI CAG_nnn'CTG
Cuts at: 137 192 297 1365 2194 2824 4262 137
Size: 55 105 1068 829 630 1438 1394
Fragments arranged by size:
1438 1394 1068 829 630 105 55
ApaI G_GGCC'C
Cuts at: 1046 1697 2201 1046
Size: 651 504 4364
Fragments arranged by size:
4364 651 504
ApaLI G'TGCA_C
Cuts at: 4357 4357
Size: 5519
ApoI r'AATT_y
Cuts at: 208 283 373 459 505 1456 1747 2523 4872
Size: 75 90 86 46 951 291 776 2349
Cuts at: 4872 5128 208
Size: 256 599
Fragments arranged by size:
2349 951 776 599 291 256 90 86
75 46
AseI AT'TA_AT
Cuts at: 20 20
Size: 5519
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5519
AvaI C'yCGr_G
Cuts at: 833 1844 2539 833
Size: 1011 695 3813
Fragments arranged by size:
3813 1011 695
AvrII C'CTAG_G
Cuts at: 1551 1551
Size: 5519
BamHI G'GATC_C
Cuts at: 2544 2544
Size: 5519
BanI G'GyrC_C
Cuts at: 2535 2949 3197 3232 2535
Size: 414 248 35 4822
Fragments arranged by size:
4822 414 248 35
BanII G_rGCy'C
Cuts at: 758 1046 1697 1713 2099 2201 2533 3563 758
Size: 288 651 16 386 102 332 1030 2714
Fragments arranged by size:
2714 1030 651 386 332 288 102 16
BbeI G_GCGC'C
Cuts at: 2953 3201 2953
Size: 248 5271
BbsI GAAGACnn'nnnn_
Cuts at: 554 1087 554
Size: 533 4986
BciVI GTATCCnnnnn_n'
Cuts at: 3417 4473 5519 3417
Size: 1056 1046 3417
Fragments arranged by size:
3417 1056 1046
BclI T'GATC_A
Cuts at: 3039 3039
Size: 5519
BglII A'GATC_T
Cuts at: 1793 3034 5071 1793
Size: 1241 2037 2241
Fragments arranged by size:
2241 2037 1241
BlpI GC'TnA_GC
Cuts at: 1034 2631 1034
Size: 1597 3922
Bme1580I G_kGCm'C
Cuts at: 1046 1697 2201 3144 3237 4361 1046
Size: 651 504 943 93 1124 2204
Fragments arranged by size:
2204 1124 943 651 504 93
BmgBI CAC'GTC
Cuts at: 2115 2115
Size: 5519
BmrI ACTGGGnnnn_n'
Cuts at: 1434 2323 2731 2812 3147 5291 1434
Size: 889 408 81 335 2144 1662
Fragments arranged by size:
2144 1662 889 408 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1533 2252 3886 1533
Size: 719 1634 3166
Fragments arranged by size:
3166 1634 719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 214 810 2282 2522 145
Size: 69 596 1472 240 3142
Fragments arranged by size:
3142 1472 596 240 69
Bpu10I CC'TnA_GC
Cuts at: 1712 1712
Size: 5519
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 2471 2695 3574 4288 4586 157
Size: 2314 224 879 714 298 1090
Fragments arranged by size:
2314 1090 879 714 298 224
BsaI GGTCTCn'nnnn_
Cuts at: 982 1294 1521 2223 5223 982
Size: 312 227 702 3000 1278
Fragments arranged by size:
3000 1278 702 312 227
BsaAI yAC'GTr
Cuts at: 2872 2872
Size: 5519
BsaBI GATnn'nnATC
Cuts at: 6 1741 3033 5070 6
Size: 1735 1292 2037 455
Fragments arranged by size:
2037 1735 1292 455
BsaHI Gr'CG_yC
Cuts at: 1009 2950 3198 3900 5396 1009
Size: 1941 248 702 1496 1132
Fragments arranged by size:
1941 1496 1132 702 248
BsaWI w'CCGG_w
Cuts at: 464 2708 3229 3907 4318 4465 4739 464
Size: 2244 521 678 411 147 274 1244
Fragments arranged by size:
2244 1244 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 773 803 773
Size: 30 5489
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2046 2046
Size: 5519
BseYI C'CCAG_C
Cuts at: 2944 3702 4367 4775 2944
Size: 758 665 408 3688
Fragments arranged by size:
3688 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1808 1808
Size: 5519
BsiEI CG_ry'CG
Cuts at: 104 3061 3107 4337 104
Size: 2957 46 1230 1286
Fragments arranged by size:
2957 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 758 1646 1713 1784 2533 3311 3501 4361 758
Size: 888 67 71 749 778 190 860 1916
Fragments arranged by size:
1916 888 860 778 749 190 71 67
BsmI GAATG_Cn'
Cuts at: 1377 1377
Size: 5519
Bsp1286I G_dGCh'C
Cuts at: 758 1046 1646 1697 1713 1784 2099 2201 2533
Size: 288 600 51 16 71 315 102 332
Cuts at: 2533 3144 3237 3311 3501 3563 4361 758
Size: 611 93 74 190 62 798 1916
Fragments arranged by size:
1916 798 611 600 332 315 288 190
102 93 74 71 62 51 16
BspEI T'CCGG_A
Cuts at: 464 464
Size: 5519
BspHI T'CATG_A
Cuts at: 1487 3951 1487
Size: 2464 3055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2569 2783 3085 3466 2569
Size: 214 302 381 4622
Fragments arranged by size:
4622 381 302 214
BsrBI CCG'CTC
Cuts at: 50 495 3811 3865 5349 5514 50
Size: 445 3316 54 1484 165 55
Fragments arranged by size:
3316 1484 445 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2412 3431 2412
Size: 1019 4500
BsrFI r'CCGG_y
Cuts at: 3517 3698 3517
Size: 181 5338
BssSI C'ACGA_G
Cuts at: 3790 4498 3790
Size: 708 4811
BstAPI GCAn_nnn'nTGC
Cuts at: 2896 2896
Size: 5519
BstBI TT'CG_AA
Cuts at: 3880 3880
Size: 5519
BstYI r'GATC_y
Cuts at: 1 1793 2343 2544 3011 3034 3369 3615 3921
Size: 1792 550 201 467 23 335 246 306
Cuts at: 3921 3933 4019 5071 1
Size: 12 86 1052 449
Fragments arranged by size:
1792 1052 550 467 449 335 306 246
201 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1465 1811 1465
Size: 346 5173
BtgI C'CryG_G
Cuts at: 107 1193 107
Size: 1086 4433
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 339 3469 3650 3813 339
Size: 3130 181 163 2045
Fragments arranged by size:
3130 2045 181 163
BtsI GCAGTG_nn'
Cuts at: 2762 2762
Size: 5519
DraI TTT'AAA
Cuts at: 2518 2518
Size: 5519
DrdI GACnn_nn'nnGTC
Cuts at: 300 3225 4569 300
Size: 2925 1344 1250
Fragments arranged by size:
2925 1344 1250
EaeI y'GGCC_r
Cuts at: 1727 3104 3278 3669 3696 5041 1727
Size: 1377 174 391 27 1345 2205
Fragments arranged by size:
2205 1377 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3104 3104
Size: 5519
EarI CTCTTCn'nnn_
Cuts at: 833 3542 3752 833
Size: 2709 210 2600
Fragments arranged by size:
2709 2600 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1829 2311 3727 4469 4615 5329 1829
Size: 482 1416 742 146 714 2019
Fragments arranged by size:
2019 1416 742 714 482 146
EcoICRI GAG'CTC
Cuts at: 756 1711 2531 756
Size: 955 820 3744
Fragments arranged by size:
3744 955 820
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 214 810 1081 2282 2522 3344 3776 4129
Size: 69 596 271 1201 240 822 432 353
Cuts at: 4129 4791 145
Size: 662 873
Fragments arranged by size:
1201 873 822 662 596 432 353 271
240 69
EcoO109I rG'GnC_Cy
Cuts at: 1042 1043 1155 2336 2658 1042
Size: 1 112 1181 322 3903
Fragments arranged by size:
3903 1181 322 112 1
EcoRI G'AATT_C
Cuts at: 2523 2523
Size: 5519
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1675 2512 3538 1675
Size: 837 1026 3656
Fragments arranged by size:
3656 1026 837
FspI TGC'GCA
Cuts at: 3300 3300
Size: 5519
HaeII r_GCGC'y
Cuts at: 254 1823 1890 2953 3201 4431 5202 254
Size: 1569 67 1063 248 1230 771 571
Fragments arranged by size:
1569 1230 1063 771 571 248 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1613 1645 1695 1727 3361 3391 3393 3423 1613
Size: 32 50 32 1634 30 2 30 3709
Fragments arranged by size:
3709 1634 50 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2558 2558
Size: 5519
KasI G'GCGC_C
Cuts at: 2949 3197 2949
Size: 248 5271
KpnI G_GTAC'C
Cuts at: 2539 2539
Size: 5519
MluI A'CGCG_T
Cuts at: 4952 4952
Size: 5519
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 816 1697 1974 2072 4278 4462 816
Size: 881 277 98 2206 184 1873
Fragments arranged by size:
2206 1873 881 277 184 98
MscI TGG'CCA
Cuts at: 3280 3280
Size: 5519
MslI CAynn'nnrTG
Cuts at: 1418 1702 1762 3508 3635 1418
Size: 284 60 1746 127 3302
Fragments arranged by size:
3302 1746 284 127 60
MspA1I CmG'CkG
Cuts at: 123 2630 2944 3304 4088 4333 123
Size: 2507 314 360 784 245 1309
Fragments arranged by size:
2507 1309 784 360 314 245
NaeI GCC'GGC
Cuts at: 3700 3700
Size: 5519
NarI GG'CG_CC
Cuts at: 2950 3198 2950
Size: 248 5271
NcoI C'CATG_G
Cuts at: 107 1193 107
Size: 1086 4433
NgoMIV G'CCGG_C
Cuts at: 3698 3698
Size: 5519
NspI r_CATG'y
Cuts at: 887 2572 3072 4675 887
Size: 1685 500 1603 1731
Fragments arranged by size:
1731 1685 1603 500
PciI A'CATG_T
Cuts at: 4671 4671
Size: 5519
PflMI CCAn_nnn'nTGG
Cuts at: 2194 2194
Size: 5519
PfoI T'CCnGG_A
Cuts at: 662 894 2298 2440 2467 2499 662
Size: 232 1404 142 27 32 3682
Fragments arranged by size:
3682 1404 232 142 32 27
PmeI GTTT'AAAC
Cuts at: 2518 2518
Size: 5519
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1131 1163 3965 3997 1131
Size: 32 2802 32 2653
Fragments arranged by size:
2802 2653 32 32
PspOMI G'GGCC_C
Cuts at: 1042 1693 2197 1042
Size: 651 504 4364
Fragments arranged by size:
4364 651 504
PstI C_TGCA'G
Cuts at: 302 1154 2141 2566 3251 302
Size: 852 987 425 685 2570
Fragments arranged by size:
2570 987 852 685 425
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5519
PvuII CAG'CTG
Cuts at: 2944 3304 2944
Size: 360 5159
RsrII CG'GwC_CG
Cuts at: 3714 3714
Size: 5519
SacI G_AGCT'C
Cuts at: 758 1713 2533 758
Size: 955 820 3744
Fragments arranged by size:
3744 955 820
SalI G'TCGA_C
Cuts at: 2556 2556
Size: 5519
SapI GCTCTTCn'nnn_
Cuts at: 3542 3752 3542
Size: 210 5309
SbfI CC_TGCA'GG
Cuts at: 2566 2566
Size: 5519
ScaI AGT'ACT
Cuts at: 1066 5036 1066
Size: 3970 1549
SexAI A'CCwGG_T
Cuts at: 2446 2446
Size: 5519
SfcI C'TryA_G
Cuts at: 33 298 601 1150 1494 2137 2562 3247 4215
Size: 265 303 549 344 643 425 685 968
Cuts at: 4215 4406 33
Size: 191 1146
Fragments arranged by size:
1146 968 685 643 549 425 344 303
265 191
SfoI GGC'GCC
Cuts at: 2951 3199 2951
Size: 248 5271
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5519
SmaI CCC'GGG
Cuts at: 835 2541 835
Size: 1706 3813
SmlI C'TyrA_G
Cuts at: 172 853 2486 2674 3589 4303 4565 172
Size: 681 1633 188 915 714 262 1126
Fragments arranged by size:
1633 1126 915 714 681 262 188
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5519
SphI G_CATG'C
Cuts at: 2572 3072 2572
Size: 500 5019
SspI AAT'ATT
Cuts at: 693 1293 1350 693
Size: 600 57 4862
Fragments arranged by size:
4862 600 57
StuI AGG'CCT
Cuts at: 2073 2073
Size: 5519
StyI C'CwwG_G
Cuts at: 107 452 488 1046 1193 1551 2653 3007 107
Size: 345 36 558 147 358 1102 354 2619
Fragments arranged by size:
2619 1102 558 358 354 345 147 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 948 3102 3235 3694 3903 948
Size: 2154 133 459 209 2564
Fragments arranged by size:
2564 2154 459 209 133
TatI w'GTAC_w
Cuts at: 1064 5034 1064
Size: 3970 1549
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 752 784 5036 5068 752
Size: 32 4252 32 1203
Fragments arranged by size:
4252 1203 32 32
Tth111I GACn'n_nGTC
Cuts at: 1086 3316 1086
Size: 2230 3289
XbaI T'CTAG_A
Cuts at: 2550 2550
Size: 5519
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1011 3014 1011
Size: 2003 3516
XmaI C'CCGG_G
Cuts at: 833 2539 833
Size: 1706 3813
XmnI GAAnn'nnTTC
Cuts at: 264 264
Size: 5519
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AgeI AleI AlfI AloI AscI BaeI
BbvCI BcgI BfrBI BglI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI