Restriction map for
pF1KB9468
(Circular) (Six-base) MAPSORT of: pF1KB9468.seq Check: 4394 from: 1 to: 9545
pF1KB9468 9545 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AatII G_ACGT'C
Cuts at: 115 115
Size: 9545
AccI GT'mk_AC
Cuts at: 5122 5372 6583 5122
Size: 250 1211 8084
Fragments arranged by size:
8084 1211 250
Acc65I G'GTAC_C
Cuts at: 6561 6561
Size: 9545
AclI AA'CG_TT
Cuts at: 2215 4220 9385 2215
Size: 2005 5165 2375
Fragments arranged by size:
5165 2375 2005
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1229 1291 1734 2221 2280 2993 3157 7370 7802
Size: 62 443 487 59 713 164 4213 432
Cuts at: 7802 8155 8817 1229
Size: 353 662 1957
Fragments arranged by size:
4213 1957 713 662 487 443 432 353
164 62 59
AfeI AGC'GCT
Cuts at: 4964 4964
Size: 9545
AflII C'TTAA_G
Cuts at: 427 1834 427
Size: 1407 8138
AflIII A'CryG_T
Cuts at: 4272 6897 8697 8978 4272
Size: 2625 1800 281 4839
Fragments arranged by size:
4839 2625 1800 281
AleI CACnn'nnGTG
Cuts at: 441 3259 5194 441
Size: 2818 1935 4792
Fragments arranged by size:
4792 2818 1935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4651 4957 4651
Size: 306 9239
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2712 2744 2712
Size: 32 9513
AlwNI CAG_nnn'CTG
Cuts at: 129 1543 3016 4438 4680 6315 6501 6850 8288
Size: 1414 1473 1422 242 1635 186 349 1438
Cuts at: 8288 129
Size: 1386
Fragments arranged by size:
1635 1473 1438 1422 1414 1386 349 242
186
ApaLI G'TGCA_C
Cuts at: 3373 8383 3373
Size: 5010 4535
ApoI r'AATT_y
Cuts at: 945 1592 2124 2491 3313 4336 4441 4648 5145
Size: 647 532 367 822 1023 105 207 497
Cuts at: 5145 6214 6549 8898 9154 945
Size: 1069 335 2349 256 1336
Fragments arranged by size:
2349 1336 1069 1023 822 647 532 497
367 335 256 207 105
AseI AT'TA_AT
Cuts at: 20 566 1855 3868 20
Size: 546 1289 2013 5697
Fragments arranged by size:
5697 2013 1289 546
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9545
AvaI C'yCGr_G
Cuts at: 726 5564 6565 726
Size: 4838 1001 3706
Fragments arranged by size:
4838 3706 1001
BamHI G'GATC_C
Cuts at: 2393 3339 6570 2393
Size: 946 3231 5368
Fragments arranged by size:
5368 3231 946
BanI G'GyrC_C
Cuts at: 2623 3786 4289 6561 6975 7223 7258 2623
Size: 1163 503 2272 414 248 35 4910
Fragments arranged by size:
4910 2272 1163 503 414 248 35
BanII G_rGCy'C
Cuts at: 2432 3254 5687 5745 6559 7589 2432
Size: 822 2433 58 814 1030 4388
Fragments arranged by size:
4388 2433 1030 822 814 58
BbeI G_GCGC'C
Cuts at: 4293 6979 7227 4293
Size: 2686 248 6611
Fragments arranged by size:
6611 2686 248
BbsI GAAGACnn'nnnn_
Cuts at: 2286 2573 2827 3874 4676 5017 2286
Size: 287 254 1047 802 341 6814
Fragments arranged by size:
6814 1047 802 341 287 254
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4399 4433 6245 6279 4399
Size: 34 1812 34 7665
Fragments arranged by size:
7665 1812 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7443 8499 9545 7443
Size: 1056 1046 7443
Fragments arranged by size:
7443 1056 1046
BclI T'GATC_A
Cuts at: 995 1884 4916 7065 995
Size: 889 3032 2149 3475
Fragments arranged by size:
3475 3032 2149 889
BfrBI ATG'CAT
Cuts at: 6251 6251
Size: 9545
BglII A'GATC_T
Cuts at: 660 1464 3681 7060 9097 660
Size: 804 2217 3379 2037 1108
Fragments arranged by size:
3379 2217 2037 1108 804
BlpI GC'TnA_GC
Cuts at: 735 1561 6657 735
Size: 826 5096 3623
Fragments arranged by size:
5096 3623 826
Bme1580I G_kGCm'C
Cuts at: 2415 3377 3634 4036 7170 7263 8387 2415
Size: 962 257 402 3134 93 1124 3573
Fragments arranged by size:
3573 3134 1124 962 402 257 93
BmgBI CAC'GTC
Cuts at: 4275 4275
Size: 9545
BmrI ACTGGGnnnn_n'
Cuts at: 2392 4333 5173 6757 6838 7173 9317 2392
Size: 1941 840 1584 81 335 2144 2620
Fragments arranged by size:
2620 2144 1941 1584 840 335 81
BmtI G_CTAG'C
Cuts at: 328 1301 328
Size: 973 8572
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3748 5289 5376 5706 6541 7912 3748
Size: 1541 87 330 835 1371 5381
Fragments arranged by size:
5381 1541 1371 835 330 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 635 2843 2917 3622 3893 4492 4780 5195
Size: 166 2208 74 705 271 599 288 415
Cuts at: 5195 5302 5346 5494 5743 5785 6511 469
Size: 107 44 148 249 42 726 3503
Fragments arranged by size:
3503 2208 726 705 599 415 288 271
249 166 148 107 74 44 42
Bpu10I CC'TnA_GC
Cuts at: 117 117
Size: 9545
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2765 6721 7600 8314 8612 2765
Size: 3956 879 714 298 3698
Fragments arranged by size:
3956 3698 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4756 5364 9249 4756
Size: 608 3885 5052
Fragments arranged by size:
5052 3885 608
BsaAI yAC'GTr
Cuts at: 6898 6898
Size: 9545
BsaBI GATnn'nnATC
Cuts at: 6 858 4501 7059 9096 6
Size: 852 3643 2558 2037 455
Fragments arranged by size:
3643 2558 2037 852 455
BsaHI Gr'CG_yC
Cuts at: 112 4290 6976 7224 7926 9422 112
Size: 4178 2686 248 702 1496 235
Fragments arranged by size:
4178 2686 1496 702 248 235
BsaWI w'CCGG_w
Cuts at: 4053 6734 7255 7933 8344 8491 8765 4053
Size: 2681 521 678 411 147 274 4833
Fragments arranged by size:
4833 2681 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 128 158 4091 4121 128
Size: 30 3933 30 5552
Fragments arranged by size:
5552 3933 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2197 2200 3013 3961 4342 5239 6324 2197
Size: 3 813 948 381 897 1085 5418
Fragments arranged by size:
5418 1085 948 897 813 381 3
BseYI C'CCAG_C
Cuts at: 122 2024 5300 5308 5744 6128 6970 7728 8393
Size: 1902 3276 8 436 384 842 758 665
Cuts at: 8393 8801 122
Size: 408 866
Fragments arranged by size:
3276 1902 866 842 758 665 436 408
384 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1990 2569 4349 4805 5083 5174 1990
Size: 579 1780 456 278 91 6361
Fragments arranged by size:
6361 1780 579 456 278 91
BsiEI CG_ry'CG
Cuts at: 104 7087 7133 8363 104
Size: 6983 46 1230 1286
Fragments arranged by size:
6983 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 755 1028 2240 2432 3377 3540 4286 5687 6559
Size: 273 1212 192 945 163 746 1401 872
Cuts at: 6559 7337 7527 8387 755
Size: 778 190 860 1913
Fragments arranged by size:
1913 1401 1212 945 872 860 778 746
273 192 190 163
BsmI GAATG_Cn'
Cuts at: 3407 5450 3407
Size: 2043 7502
BsmBI CGTCTCn'nnnn_
Cuts at: 1179 2247 5527 1179
Size: 1068 3280 5197
Fragments arranged by size:
5197 3280 1068
Bsp1286I G_dGCh'C
Cuts at: 755 1028 2240 2415 2432 3254 3377 3540 3634
Size: 273 1212 175 17 822 123 163 94
Cuts at: 3634 4036 4286 5687 5745 6559 7170 7263 7337
Size: 402 250 1401 58 814 611 93 74
Cuts at: 7337 7527 7589 8387 755
Size: 190 62 798 1913
Fragments arranged by size:
1913 1401 1212 822 814 798 611 402
273 250 190 175 163 123 94 93
74 62 58 17
BspEI T'CCGG_A
Cuts at: 4053 4053
Size: 9545
BspHI T'CATG_A
Cuts at: 1622 7977 1622
Size: 6355 3190
BspMI ACCTGCnnnn'nnnn_
Cuts at: 5384 6595 6809 7111 7492 5384
Size: 1211 214 302 381 7437
Fragments arranged by size:
7437 1211 381 302 214
BsrBI CCG'CTC
Cuts at: 50 7837 7891 9375 9540 50
Size: 7787 54 1484 165 55
Fragments arranged by size:
7787 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 500 2810 5933 7457 500
Size: 2310 3123 1524 2588
Fragments arranged by size:
3123 2588 2310 1524
BsrFI r'CCGG_y
Cuts at: 5971 7543 7724 5971
Size: 1572 181 7792
Fragments arranged by size:
7792 1572 181
BssHII G'CGCG_C
Cuts at: 4839 4841 5246 4839
Size: 2 405 9138
Fragments arranged by size:
9138 405 2
BssSI C'ACGA_G
Cuts at: 523 7816 8524 523
Size: 7293 708 1544
Fragments arranged by size:
7293 1544 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1155 3660 6922 1155
Size: 2505 3262 3778
Fragments arranged by size:
3778 3262 2505
BstBI TT'CG_AA
Cuts at: 7906 7906
Size: 9545
BstEII G'GTnAC_C
Cuts at: 5268 5268
Size: 9545
BstXI CCAn_nnnn'nTGG
Cuts at: 5355 5355
Size: 9545
BstYI r'GATC_y
Cuts at: 1 660 1464 2133 2393 3239 3339 3681 5166
Size: 659 804 669 260 846 100 342 1485
Cuts at: 5166 6570 7037 7060 7395 7641 7947 7959 8045
Size: 1404 467 23 335 246 306 12 86
Cuts at: 8045 9097 1
Size: 1052 449
Fragments arranged by size:
1485 1404 1052 846 804 669 659 467
449 342 335 306 260 246 100 86
23 12
BstZ17I GTA'TAC
Cuts at: 5123 5123
Size: 9545
Bsu36I CC'TnA_GG
Cuts at: 206 6341 206
Size: 6135 3410
BtgI C'CryG_G
Cuts at: 926 2453 3278 4799 5813 6272 926
Size: 1527 825 1521 1014 459 4199
Fragments arranged by size:
4199 1527 1521 1014 825 459
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 807 6237 7495 7676 7839 807
Size: 5430 1258 181 163 2513
Fragments arranged by size:
5430 2513 1258 181 163
BtsI GCAGTG_nn'
Cuts at: 648 2351 2763 3558 4565 5189 6788 648
Size: 1703 412 795 1007 624 1599 3405
Fragments arranged by size:
3405 1703 1599 1007 795 624 412
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1883 1918 1883
Size: 35 9510
DraI TTT'AAA
Cuts at: 2261 3332 3561 4647 4736 6544 2261
Size: 1071 229 1086 89 1808 5262
Fragments arranged by size:
5262 1808 1086 1071 229 89
DraIII CAC_nnn'GTG
Cuts at: 3535 4327 5982 3535
Size: 792 1655 7098
Fragments arranged by size:
7098 1655 792
DrdI GACnn_nn'nnGTC
Cuts at: 7251 8595 7251
Size: 1344 8201
EaeI y'GGCC_r
Cuts at: 215 1598 2450 5054 5291 5973 6269 7130 7304
Size: 1383 852 2604 237 682 296 861 174
Cuts at: 7304 7695 7722 9067 215
Size: 391 27 1345 693
Fragments arranged by size:
2604 1383 1345 861 852 693 682 391
296 237 174 27
EagI C'GGCC_G
Cuts at: 7130 7130
Size: 9545
EarI CTCTTCn'nnn_
Cuts at: 260 1148 1307 1859 2420 3340 4220 4742 5675
Size: 888 159 552 561 920 880 522 933
Cuts at: 5675 6203 7568 7778 260
Size: 528 1365 210 2027
Fragments arranged by size:
2027 1365 933 920 888 880 561 552
528 522 210 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3973 6292 7753 8495 8641 9355 3973
Size: 2319 1461 742 146 714 4163
Fragments arranged by size:
4163 2319 1461 742 714 146
EcoICRI GAG'CTC
Cuts at: 2430 5685 6557 2430
Size: 3255 872 5418
Fragments arranged by size:
5418 3255 872
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 635 1229 1291 1734 2221 2280 2843 2917
Size: 166 594 62 443 487 59 563 74
Cuts at: 2917 2993 3157 3622 3893 4492 4780 5195 5302
Size: 76 164 465 271 599 288 415 107
Cuts at: 5302 5346 5494 5743 5785 6511 7370 7802 8155
Size: 44 148 249 42 726 859 432 353
Cuts at: 8155 8817 469
Size: 662 1197
Fragments arranged by size:
1197 859 726 662 599 594 563 487
465 443 432 415 353 288 271 249
166 164 148 107 76 74 62 59
44 42
EcoO109I rG'GnC_Cy
Cuts at: 2937 5727 5846 6684 2937
Size: 2790 119 838 5798
Fragments arranged by size:
5798 2790 838 119
EcoRI G'AATT_C
Cuts at: 945 1592 6549 945
Size: 647 4957 3941
Fragments arranged by size:
4957 3941 647
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 289 1257 1270 1296 2053 6090 7564 289
Size: 968 13 26 757 4037 1474 2270
Fragments arranged by size:
4037 2270 1474 968 757 26 13
FspI TGC'GCA
Cuts at: 7326 7326
Size: 9545
HaeII r_GCGC'y
Cuts at: 790 4293 4966 5290 6979 7227 8457 9228 790
Size: 3503 673 324 1689 248 1230 771 1107
Fragments arranged by size:
3503 1689 1230 1107 771 673 324 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 128 160 793 825 888 920 1758 1790 1876
Size: 32 633 32 63 32 838 32 86
Cuts at: 1876 1908 1936 1968 2173 2205 2769 2801 2961
Size: 32 28 32 205 32 564 32 160
Cuts at: 2961 2993 3673 3705 4091 4123 4707 4739 6156
Size: 32 680 32 386 32 584 32 1417
Cuts at: 6156 6188 7387 7417 7419 7449 128
Size: 32 1199 30 2 30 2224
Fragments arranged by size:
2224 1417 1199 838 680 633 584 564
386 205 160 86 63 32 32 32
32 32 32 32 32 32 32 32
32 32 30 30 28 2
HincII GTy'rAC
Cuts at: 3183 5909 6584 3183
Size: 2726 675 6144
Fragments arranged by size:
6144 2726 675
HindIII A'AGCT_T
Cuts at: 2323 3484 4491 4856 5131 2323
Size: 1161 1007 365 275 6737
Fragments arranged by size:
6737 1161 1007 365 275
KasI G'GCGC_C
Cuts at: 4289 6975 7223 4289
Size: 2686 248 6611
Fragments arranged by size:
6611 2686 248
KpnI G_GTAC'C
Cuts at: 6565 6565
Size: 9545
MluI A'CGCG_T
Cuts at: 8978 8978
Size: 9545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 356 3684 5754 8304 8488 356
Size: 3328 2070 2550 184 1413
Fragments arranged by size:
3328 2550 2070 1413 184
MscI TGG'CCA
Cuts at: 1600 5056 5293 6271 7306 1600
Size: 3456 237 978 1035 3839
Fragments arranged by size:
3839 3456 1035 978 237
MslI CAynn'nnrTG
Cuts at: 441 2668 3259 4013 4912 5194 7534 7661 441
Size: 2227 591 754 899 282 2340 127 2325
Fragments arranged by size:
2340 2325 2227 899 754 591 282 127
MspA1I CmG'CkG
Cuts at: 129 1846 3259 6186 6656 6970 7330 8114 8359
Size: 1717 1413 2927 470 314 360 784 245
Cuts at: 8359 129
Size: 1315
Fragments arranged by size:
2927 1717 1413 1315 784 470 360 314
245
NaeI GCC'GGC
Cuts at: 5973 7726 5973
Size: 1753 7792
NarI GG'CG_CC
Cuts at: 4290 6976 7224 4290
Size: 2686 248 6611
Fragments arranged by size:
6611 2686 248
NcoI C'CATG_G
Cuts at: 926 3278 4799 5813 6272 926
Size: 2352 1521 1014 459 4199
Fragments arranged by size:
4199 2352 1521 1014 459
NgoMIV G'CCGG_C
Cuts at: 5971 7724 5971
Size: 1753 7792
NheI G'CTAG_C
Cuts at: 324 1297 324
Size: 973 8572
NsiI A_TGCA'T
Cuts at: 6253 6253
Size: 9545
NspI r_CATG'y
Cuts at: 1158 6598 7098 8701 1158
Size: 5440 500 1603 2002
Fragments arranged by size:
5440 2002 1603 500
PacI TTA_AT'TAA
Cuts at: 1859 1859
Size: 9545
PciI A'CATG_T
Cuts at: 8697 8697
Size: 9545
PflMI CCAn_nnn'nTGG
Cuts at: 1293 3916 1293
Size: 2623 6922
PfoI T'CCnGG_A
Cuts at: 3737 4277 5002 5720 6218 3737
Size: 540 725 718 498 7064
Fragments arranged by size:
7064 725 718 540 498
PmeI GTTT'AAAC
Cuts at: 6544 6544
Size: 9545
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3937 3969 7991 8023 3937
Size: 32 4022 32 5459
Fragments arranged by size:
5459 4022 32 32
PsiI TTA'TAA
Cuts at: 3364 6139 3364
Size: 2775 6770
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 627 659 2891 2923 6088 6120 627
Size: 32 2232 32 3165 32 4052
Fragments arranged by size:
4052 3165 2232 32 32 32
PstI C_TGCA'G
Cuts at: 801 1034 2346 2758 2777 3752 4991 5402 6592
Size: 233 1312 412 19 975 1239 411 1190
Cuts at: 6592 7277 801
Size: 685 3069
Fragments arranged by size:
3069 1312 1239 1190 975 685 412 411
233 19
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9545
PvuII CAG'CTG
Cuts at: 129 3259 6186 6970 7330 129
Size: 3130 2927 784 360 2344
Fragments arranged by size:
3130 2927 2344 784 360
RsrII CG'GwC_CG
Cuts at: 7740 7740
Size: 9545
SacI G_AGCT'C
Cuts at: 2432 5687 6559 2432
Size: 3255 872 5418
Fragments arranged by size:
5418 3255 872
SalI G'TCGA_C
Cuts at: 6582 6582
Size: 9545
SapI GCTCTTCn'nnn_
Cuts at: 2420 5675 7568 7778 2420
Size: 3255 1893 210 4187
Fragments arranged by size:
4187 3255 1893 210
SbfI CC_TGCA'GG
Cuts at: 6592 6592
Size: 9545
ScaI AGT'ACT
Cuts at: 3170 4513 9062 3170
Size: 1343 4549 3653
Fragments arranged by size:
4549 3653 1343
SexAI A'CCwGG_T
Cuts at: 6008 6008
Size: 9545
SfcI C'TryA_G
Cuts at: 33 797 1030 2342 2754 2773 3058 3748 4202
Size: 764 233 1312 412 19 285 690 454
Cuts at: 4202 4597 4987 5398 6588 7273 8241 8432 33
Size: 395 390 411 1190 685 968 191 1146
Fragments arranged by size:
1312 1190 1146 968 764 690 685 454
412 411 395 390 285 233 191 19
SfoI GGC'GCC
Cuts at: 4291 6977 7225 4291
Size: 2686 248 6611
Fragments arranged by size:
6611 2686 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9545
SmaI CCC'GGG
Cuts at: 728 6567 728
Size: 5839 3706
SmlI C'TyrA_G
Cuts at: 427 1834 2780 6700 7615 8329 8591 427
Size: 1407 946 3920 915 714 262 1381
Fragments arranged by size:
3920 1407 1381 946 915 714 262
SpeI A'CTAG_T
Cuts at: 67 5485 67
Size: 5418 4127
SphI G_CATG'C
Cuts at: 6598 7098 6598
Size: 500 9045
SspI AAT'ATT
Cuts at: 536 4049 536
Size: 3513 6032
StuI AGG'CCT
Cuts at: 4742 4742
Size: 9545
StyI C'CwwG_G
Cuts at: 335 926 1053 3278 3626 4799 5387 5813 6272
Size: 591 127 2225 348 1173 588 426 459
Cuts at: 6272 6679 7033 335
Size: 407 354 2847
Fragments arranged by size:
2847 2225 1173 591 588 459 426 407
354 348 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2167 4315 7128 7261 7720 7929 2167
Size: 2148 2813 133 459 209 3783
Fragments arranged by size:
3783 2813 2148 459 209 133
TatI w'GTAC_w
Cuts at: 253 3168 4511 9060 253
Size: 2915 1343 4549 738
Fragments arranged by size:
4549 2915 1343 738
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5552 5584 9062 9094 5552
Size: 32 3478 32 6003
Fragments arranged by size:
6003 3478 32 32
Tth111I GACn'n_nGTC
Cuts at: 7342 7342
Size: 9545
XbaI T'CTAG_A
Cuts at: 172 2676 3351 3684 6576 172
Size: 2504 675 333 2892 3141
Fragments arranged by size:
3141 2892 2504 675 333
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2405 2941 7040 2405
Size: 536 4099 4910
Fragments arranged by size:
4910 4099 536
XmaI C'CCGG_G
Cuts at: 726 6565 726
Size: 5839 3706
XmnI GAAnn'nnTTC
Cuts at: 1379 2659 3473 3692 4547 5450 6425 1379
Size: 1280 814 219 855 903 975 4499
Fragments arranged by size:
4499 1280 975 903 855 814 219
ZraI GAC'GTC
Cuts at: 113 113
Size: 9545
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NsiI NspI PacI PciI PflMI PfoI
PmeI PpiI PsiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AgeI AhdI ApaI AscI AvrII BaeI BbvCI
BglI BsiWI BsrGI ClaI EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NotI NruI PasI PmlI PpuMI
PshAI PspOMI PspXI SacII SanDI SfiI SgrAI SnaBI
SrfI SwaI XhoI