Restriction map for
pF1KB9465
(Circular) (Six-base) MAPSORT of: pF1KB9465.seq Check: 2772 from: 1 to: 6224
pF1KB9465 6224 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 126 1379 1995 2242 126
Size: 1253 616 247 4108
Fragments arranged by size:
4108 1253 616 247
AccI GT'mk_AC
Cuts at: 3262 3262
Size: 6224
Acc65I G'GTAC_C
Cuts at: 3240 3240
Size: 6224
AclI AA'CG_TT
Cuts at: 6064 6064
Size: 6224
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 460 1342 1738 2462 4049 4481 4834 5496 460
Size: 882 396 724 1587 432 353 662 1188
Fragments arranged by size:
1587 1188 882 724 662 432 396 353
AflIII A'CryG_T
Cuts at: 728 3576 5376 5657 728
Size: 2848 1800 281 1295
Fragments arranged by size:
2848 1800 1295 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 391 396 780 1748 391
Size: 5 384 968 4867
Fragments arranged by size:
4867 968 384 5
AlwNI CAG_nnn'CTG
Cuts at: 1371 1761 2142 3529 4967 1371
Size: 390 381 1387 1438 2628
Fragments arranged by size:
2628 1438 1387 390 381
ApaLI G'TGCA_C
Cuts at: 5062 5062
Size: 6224
ApoI r'AATT_y
Cuts at: 1128 1399 2803 3228 5577 5833 1128
Size: 271 1404 425 2349 256 1519
Fragments arranged by size:
2349 1519 1404 425 271 256
AseI AT'TA_AT
Cuts at: 20 251 20
Size: 231 5993
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6224
AvaI C'yCGr_G
Cuts at: 2531 2924 3244 2531
Size: 393 320 5511
Fragments arranged by size:
5511 393 320
BamHI G'GATC_C
Cuts at: 2573 3249 2573
Size: 676 5548
BanI G'GyrC_C
Cuts at: 138 304 2224 3240 3654 3902 3937 138
Size: 166 1920 1016 414 248 35 2425
Fragments arranged by size:
2425 1920 1016 414 248 166 35
BanII G_rGCy'C
Cuts at: 1427 2247 2408 3238 4268 1427
Size: 820 161 830 1030 3383
Fragments arranged by size:
3383 1030 830 820 161
BbeI G_GCGC'C
Cuts at: 3658 3906 3658
Size: 248 5976
BbsI GAAGACnn'nnnn_
Cuts at: 1297 1693 2162 1297
Size: 396 469 5359
Fragments arranged by size:
5359 469 396
BbvCI CC'TCA_GC
Cuts at: 1011 3152 1011
Size: 2141 4083
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2197 2231 2905 2939 2197
Size: 34 674 34 5482
Fragments arranged by size:
5482 674 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4122 5178 6224 4122
Size: 1056 1046 4122
Fragments arranged by size:
4122 1056 1046
BclI T'GATC_A
Cuts at: 3744 3744
Size: 6224
BglI GCCn_nnn'nGGC
Cuts at: 2314 2737 2314
Size: 423 5801
BglII A'GATC_T
Cuts at: 918 1350 3739 5776 918
Size: 432 2389 2037 1366
Fragments arranged by size:
2389 2037 1366 432
BlpI GC'TnA_GC
Cuts at: 163 1457 2521 3336 163
Size: 1294 1064 815 3051
Fragments arranged by size:
3051 1294 1064 815
Bme1580I G_kGCm'C
Cuts at: 143 307 327 3849 3942 5066 143
Size: 164 20 3522 93 1124 1301
Fragments arranged by size:
3522 1301 1124 164 93 20
BmgBI CAC'GTC
Cuts at: 639 1332 639
Size: 693 5531
BmrI ACTGGGnnnn_n'
Cuts at: 310 1782 1812 3118 3436 3517 3852 5996 310
Size: 1472 30 1306 318 81 335 2144 538
Fragments arranged by size:
2144 1472 1306 538 335 318 81 30
BmtI G_CTAG'C
Cuts at: 2846 2846
Size: 6224
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4591 4591
Size: 6224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 647 703 918 1666 2140 2151 2344 2623
Size: 436 56 215 748 474 11 193 279
Cuts at: 2623 211
Size: 3812
Fragments arranged by size:
3812 748 474 436 279 215 193 56
11
Bpu10I CC'TnA_GC
Cuts at: 499 1011 1426 2252 2377 3152 499
Size: 512 415 826 125 775 3571
Fragments arranged by size:
3571 826 775 512 415 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1475 1676 2362 2997 3400 4279 4993 5291 1475
Size: 201 686 635 403 879 714 298 2408
Fragments arranged by size:
2408 879 714 686 635 403 298 201
BsaI GGTCTCn'nnnn_
Cuts at: 5928 5928
Size: 6224
BsaAI yAC'GTr
Cuts at: 3577 3577
Size: 6224
BsaBI GATnn'nnATC
Cuts at: 6 3738 5775 6
Size: 3732 2037 455
Fragments arranged by size:
3732 2037 455
BsaHI Gr'CG_yC
Cuts at: 2018 2210 3655 3903 4605 6101 2018
Size: 192 1445 248 702 1496 2141
Fragments arranged by size:
2141 1496 1445 702 248 192
BsaWI w'CCGG_w
Cuts at: 3413 3934 4612 5023 5170 5444 3413
Size: 521 678 411 147 274 4193
Fragments arranged by size:
4193 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1639 1669 2065 2095 2302 2332 2744 2774 1639
Size: 30 396 30 207 30 412 30 5089
Fragments arranged by size:
5089 412 396 207 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 403 1885 2033 2068 2416 2452 2527 2824 403
Size: 1482 148 35 348 36 75 297 3803
Fragments arranged by size:
3803 1482 348 297 148 75 36 35
BseYI C'CCAG_C
Cuts at: 184 858 974 2059 2442 2747 2852 2906 3158
Size: 674 116 1085 383 305 105 54 252
Cuts at: 3158 3649 4407 5072 5480 184
Size: 491 758 665 408 928
Fragments arranged by size:
1085 928 758 674 665 491 408 383
305 252 116 105 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 543 543
Size: 6224
BsiEI CG_ry'CG
Cuts at: 104 1388 3766 3812 5042 104
Size: 1284 2378 46 1230 1286
Fragments arranged by size:
2378 1286 1284 1230 46
BsiHKAI G_wGCw'C
Cuts at: 380 1427 2408 2618 3238 4016 4206 5066 380
Size: 1047 981 210 620 778 190 860 1538
Fragments arranged by size:
1538 1047 981 860 778 620 210 190
BsmI GAATG_Cn'
Cuts at: 1578 2352 1578
Size: 774 5450
Bsp1286I G_dGCh'C
Cuts at: 143 307 327 380 1427 2247 2408 2618 3238
Size: 164 20 53 1047 820 161 210 620
Cuts at: 3238 3849 3942 4016 4206 4268 5066 143
Size: 611 93 74 190 62 798 1301
Fragments arranged by size:
1301 1047 820 798 620 611 210 190
164 161 93 74 62 53 20
BspHI T'CATG_A
Cuts at: 2829 4656 2829
Size: 1827 4397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 126 602 950 1379 1568 1995 2242 3274 3488
Size: 476 348 429 189 427 247 1032 214
Cuts at: 3488 3790 4171 126
Size: 302 381 2179
Fragments arranged by size:
2179 1032 476 429 427 381 348 302
247 214 189
BsrBI CCG'CTC
Cuts at: 50 219 737 932 1836 2660 4516 4570 6054
Size: 169 518 195 904 824 1856 54 1484
Cuts at: 6054 6219 50
Size: 165 55
Fragments arranged by size:
1856 1484 904 824 518 195 169 165
55 54
BsrDI GCAATG_nn'
Cuts at: 2213 4136 2213
Size: 1923 4301
BsrFI r'CCGG_y
Cuts at: 167 2947 4222 4403 167
Size: 2780 1275 181 1988
Fragments arranged by size:
2780 1988 1275 181
BssSI C'ACGA_G
Cuts at: 4495 5203 4495
Size: 708 5516
BstAPI GCAn_nnn'nTGC
Cuts at: 1371 3601 1371
Size: 2230 3994
BstBI TT'CG_AA
Cuts at: 4585 4585
Size: 6224
BstEII G'GTnAC_C
Cuts at: 1581 3204 1581
Size: 1623 4601
BstXI CCAn_nnnn'nTGG
Cuts at: 2421 2742 2763 2421
Size: 321 21 5882
Fragments arranged by size:
5882 321 21
BstYI r'GATC_y
Cuts at: 1 918 968 1350 2573 3249 3716 3739 4074
Size: 917 50 382 1223 676 467 23 335
Cuts at: 4074 4320 4626 4638 4724 5776 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1223 1052 917 676 467 449 382 335
306 246 86 50 23 12
BtgI C'CryG_G
Cuts at: 107 1598 2084 2498 107
Size: 1491 486 414 3833
Fragments arranged by size:
3833 1491 486 414
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4174 4355 4518 4174
Size: 181 163 5880
Fragments arranged by size:
5880 181 163
BtsI GCAGTG_nn'
Cuts at: 148 380 769 1182 2942 3190 3467 148
Size: 232 389 413 1760 248 277 2905
Fragments arranged by size:
2905 1760 413 389 277 248 232
DraI TTT'AAA
Cuts at: 3223 3223
Size: 6224
DrdI GACnn_nn'nnGTC
Cuts at: 336 823 2548 3930 5274 336
Size: 487 1725 1382 1344 1286
Fragments arranged by size:
1725 1382 1344 1286 487
EaeI y'GGCC_r
Cuts at: 119 513 1355 1385 1601 1922 2471 2501 3809
Size: 394 842 30 216 321 549 30 1308
Cuts at: 3809 3983 4374 4401 5746 119
Size: 174 391 27 1345 597
Fragments arranged by size:
1345 1308 842 597 549 394 391 321
216 174 30 30 27
EagI C'GGCC_G
Cuts at: 1385 3809 1385
Size: 2424 3800
EarI CTCTTCn'nnn_
Cuts at: 706 841 1892 2324 2984 4247 4457 706
Size: 135 1051 432 660 1263 210 2473
Fragments arranged by size:
2473 1263 1051 660 432 210 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 214 4432 5174 5320 6034 214
Size: 4218 742 146 714 404
Fragments arranged by size:
4218 742 714 404 146
EcoICRI GAG'CTC
Cuts at: 1425 2406 3236 1425
Size: 981 830 4413
Fragments arranged by size:
4413 981 830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 460 647 703 918 1342 1666 1738 2140
Size: 249 187 56 215 424 324 72 402
Cuts at: 2140 2151 2344 2462 2623 4049 4481 4834 5496
Size: 11 193 118 161 1426 432 353 662
Cuts at: 5496 211
Size: 939
Fragments arranged by size:
1426 939 662 432 424 402 353 324
249 215 193 187 161 118 72 56
11
EcoNI CCTnn'n_nnAGG
Cuts at: 502 1090 2599 502
Size: 588 1509 4127
Fragments arranged by size:
4127 1509 588
EcoO109I rG'GnC_Cy
Cuts at: 2448 2634 3363 2448
Size: 186 729 5309
Fragments arranged by size:
5309 729 186
EcoRI G'AATT_C
Cuts at: 1399 3228 1399
Size: 1829 4395
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 852 1248 4243 852
Size: 396 2995 2833
Fragments arranged by size:
2995 2833 396
FspI TGC'GCA
Cuts at: 4005 4005
Size: 6224
HaeII r_GCGC'y
Cuts at: 185 3658 3906 5136 5907 185
Size: 3473 248 1230 771 502
Fragments arranged by size:
3473 1230 771 502 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1048 1080 1864 1896 2124 2156 2411 2443 2744
Size: 32 784 32 228 32 255 32 301
Cuts at: 2744 2776 3039 3071 4066 4096 4098 4128 1048
Size: 32 263 32 995 30 2 30 3144
Fragments arranged by size:
3144 995 784 301 263 255 228 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 348 659 3263 348
Size: 311 2604 3309
Fragments arranged by size:
3309 2604 311
HindIII A'AGCT_T
Cuts at: 829 829
Size: 6224
HpaI GTT'AAC
Cuts at: 659 659
Size: 6224
KasI G'GCGC_C
Cuts at: 3654 3902 3654
Size: 248 5976
KpnI G_GTAC'C
Cuts at: 3244 3244
Size: 6224
MluI A'CGCG_T
Cuts at: 5657 5657
Size: 6224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1637 4983 5167 1637
Size: 3346 184 2694
Fragments arranged by size:
3346 2694 184
MscI TGG'CCA
Cuts at: 121 515 1603 1924 3985 121
Size: 394 1088 321 2061 2360
Fragments arranged by size:
2360 2061 1088 394 321
MslI CAynn'nnrTG
Cuts at: 812 1108 2362 2761 4213 4340 812
Size: 296 1254 399 1452 127 2696
Fragments arranged by size:
2696 1452 1254 399 296 127
MspA1I CmG'CkG
Cuts at: 519 615 780 862 978 1305 1320 1437 1461
Size: 96 165 82 116 327 15 117 24
Cuts at: 1461 1683 1701 2054 2105 2482 2776 2910 3335
Size: 222 18 353 51 377 294 134 425
Cuts at: 3335 3649 4009 4793 5038 519
Size: 314 360 784 245 1705
Fragments arranged by size:
1705 784 425 377 360 353 327 314
294 245 222 165 134 117 116 96
82 51 24 18 15
NaeI GCC'GGC
Cuts at: 169 2949 4405 169
Size: 2780 1456 1988
Fragments arranged by size:
2780 1988 1456
NarI GG'CG_CC
Cuts at: 3655 3903 3655
Size: 248 5976
NcoI C'CATG_G
Cuts at: 107 1598 107
Size: 1491 4733
NgoMIV G'CCGG_C
Cuts at: 167 2947 4403 167
Size: 2780 1456 1988
Fragments arranged by size:
2780 1988 1456
NheI G'CTAG_C
Cuts at: 2842 2842
Size: 6224
NspI r_CATG'y
Cuts at: 732 3277 3777 5380 732
Size: 2545 500 1603 1576
Fragments arranged by size:
2545 1603 1576 500
PasI CC'CwG_GG
Cuts at: 2414 2414
Size: 6224
PciI A'CATG_T
Cuts at: 728 5376 728
Size: 4648 1576
PflMI CCAn_nnn'nTGG
Cuts at: 2142 2859 3048 2142
Size: 717 189 5318
Fragments arranged by size:
5318 717 189
PfoI T'CCnGG_A
Cuts at: 1987 1987
Size: 6224
PmeI GTTT'AAAC
Cuts at: 3223 3223
Size: 6224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 683 715 2030 2062 4670 4702 683
Size: 32 1315 32 2608 32 2205
Fragments arranged by size:
2608 2205 1315 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2448 2634 2448
Size: 186 6038
PstI C_TGCA'G
Cuts at: 599 805 947 1056 1319 1904 2070 2915 3271
Size: 206 142 109 263 585 166 845 356
Cuts at: 3271 3956 599
Size: 685 2867
Fragments arranged by size:
2867 845 685 585 356 263 206 166
142 109
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6224
PvuII CAG'CTG
Cuts at: 519 615 862 978 1320 1701 2054 2105 2482
Size: 96 247 116 342 381 353 51 377
Cuts at: 2482 2776 2910 3649 4009 519
Size: 294 134 739 360 2734
Fragments arranged by size:
2734 739 381 377 360 353 342 294
247 134 116 96 51
RsrII CG'GwC_CG
Cuts at: 4419 4419
Size: 6224
SacI G_AGCT'C
Cuts at: 1427 2408 3238 1427
Size: 981 830 4413
Fragments arranged by size:
4413 981 830
SalI G'TCGA_C
Cuts at: 3261 3261
Size: 6224
SapI GCTCTTCn'nnn_
Cuts at: 1892 4247 4457 1892
Size: 2355 210 3659
Fragments arranged by size:
3659 2355 210
SbfI CC_TGCA'GG
Cuts at: 3271 3271
Size: 6224
ScaI AGT'ACT
Cuts at: 949 5741 949
Size: 4792 1432
SexAI A'CCwGG_T
Cuts at: 2450 2450
Size: 6224
SfcI C'TryA_G
Cuts at: 33 595 801 943 1052 1315 1900 2066 2911
Size: 562 206 142 109 263 585 166 845
Cuts at: 2911 3267 3952 4920 5111 33
Size: 356 685 968 191 1146
Fragments arranged by size:
1146 968 845 685 585 562 356 263
206 191 166 142 109
SfoI GGC'GCC
Cuts at: 3656 3904 3656
Size: 248 5976
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6224
SmaI CCC'GGG
Cuts at: 3246 3246
Size: 6224
SmlI C'TyrA_G
Cuts at: 1490 1691 2341 3012 3379 4294 5008 5270 1490
Size: 201 650 671 367 915 714 262 2444
Fragments arranged by size:
2444 915 714 671 650 367 262 201
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6224
SphI G_CATG'C
Cuts at: 3277 3777 3277
Size: 500 5724
StuI AGG'CCT
Cuts at: 1060 2278 2920 1060
Size: 1218 642 4364
Fragments arranged by size:
4364 1218 642
StyI C'CwwG_G
Cuts at: 107 155 1598 2577 2735 2870 3358 3712 107
Size: 48 1443 979 158 135 488 354 2619
Fragments arranged by size:
2619 1443 979 488 354 158 135 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 322 1008 2287 3807 3940 4399 4608 322
Size: 686 1279 1520 133 459 209 1938
Fragments arranged by size:
1938 1520 1279 686 459 209 133
TatI w'GTAC_w
Cuts at: 947 1529 5739 947
Size: 582 4210 1432
Fragments arranged by size:
4210 1432 582
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 558 590 983 1015 1927 1959 2065 2097 5741
Size: 32 393 32 912 32 106 32 3644
Cuts at: 5741 5773 558
Size: 32 1009
Fragments arranged by size:
3644 1009 912 393 106 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4021 4021
Size: 6224
XbaI T'CTAG_A
Cuts at: 1710 3255 1710
Size: 1545 4679
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2187 2763 3719 114
Size: 2073 576 956 2619
Fragments arranged by size:
2619 2073 956 576
XmaI C'CCGG_G
Cuts at: 3244 3244
Size: 6224
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI ApaI
AscI AvrII BaeI BfrBI BsiWI BsmBI BspEI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI MfeI NdeI NotI NruI NsiI PacI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI