Restriction map for
pF1KB9461
(Circular) (Six-base) MAPSORT of: pF1KB9461.seq Check: 4271 from: 1 to: 4787
pF1KB9461 4787 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1078 1289 1078
Size: 211 4576
AccI GT'mk_AC
Cuts at: 1825 1825
Size: 4787
Acc65I G'GTAC_C
Cuts at: 1803 1803
Size: 4787
AclI AA'CG_TT
Cuts at: 4627 4627
Size: 4787
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 652 745 2612 3044 3397 4059 652
Size: 93 1867 432 353 662 1380
Fragments arranged by size:
1867 1380 662 432 353 93
AflII C'TTAA_G
Cuts at: 1288 1288
Size: 4787
AflIII A'CryG_T
Cuts at: 377 2139 3939 4220 377
Size: 1762 1800 281 944
Fragments arranged by size:
1800 1762 944 281
AleI CACnn'nnGTG
Cuts at: 1483 1483
Size: 4787
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1379 1411 1379
Size: 32 4755
AlwNI CAG_nnn'CTG
Cuts at: 741 2092 3530 741
Size: 1351 1438 1998
Fragments arranged by size:
1998 1438 1351
ApaLI G'TGCA_C
Cuts at: 1572 3625 1572
Size: 2053 2734
ApoI r'AATT_y
Cuts at: 473 778 808 982 1342 1791 4140 4396 473
Size: 305 30 174 360 449 2349 256 864
Fragments arranged by size:
2349 864 449 360 305 256 174 30
AseI AT'TA_AT
Cuts at: 20 20
Size: 4787
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4787
AvaI C'yCGr_G
Cuts at: 1027 1807 1027
Size: 780 4007
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1702 1735 1702
Size: 33 4754
BamHI G'GATC_C
Cuts at: 272 1812 272
Size: 1540 3247
BanI G'GyrC_C
Cuts at: 802 1803 2217 2465 2500 802
Size: 1001 414 248 35 3089
Fragments arranged by size:
3089 1001 414 248 35
BanII G_rGCy'C
Cuts at: 175 1801 2831 175
Size: 1626 1030 2131
Fragments arranged by size:
2131 1626 1030
BbeI G_GCGC'C
Cuts at: 2221 2469 2221
Size: 248 4539
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 784 818 784
Size: 34 4753
BciVI GTATCCnnnnn_n'
Cuts at: 537 2685 3741 4787 537
Size: 2148 1056 1046 537
Fragments arranged by size:
2148 1056 1046 537
BclI T'GATC_A
Cuts at: 614 2307 614
Size: 1693 3094
BglII A'GATC_T
Cuts at: 1109 2302 4339 1109
Size: 1193 2037 1557
Fragments arranged by size:
2037 1557 1193
BlpI GC'TnA_GC
Cuts at: 1899 1899
Size: 4787
Bme1580I G_kGCm'C
Cuts at: 1576 2412 2505 3629 1576
Size: 836 93 1124 2734
Fragments arranged by size:
2734 1124 836 93
BmrI ACTGGGnnnn_n'
Cuts at: 1999 2080 2415 4559 1999
Size: 81 335 2144 2227
Fragments arranged by size:
2227 2144 335 81
BmtI G_CTAG'C
Cuts at: 1532 1532
Size: 4787
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3154 3154
Size: 4787
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 661 783 1619 347
Size: 314 122 836 3515
Fragments arranged by size:
3515 836 314 122
Bpu10I CC'TnA_GC
Cuts at: 604 604
Size: 4787
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 811 1963 2842 3556 3854 811
Size: 1152 879 714 298 1744
Fragments arranged by size:
1744 1152 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 457 837 4491 457
Size: 380 3654 753
Fragments arranged by size:
3654 753 380
BsaAI yAC'GTr
Cuts at: 2140 2140
Size: 4787
BsaBI GATnn'nnATC
Cuts at: 6 2301 4338 6
Size: 2295 2037 455
Fragments arranged by size:
2295 2037 455
BsaHI Gr'CG_yC
Cuts at: 2218 2466 3168 4664 2218
Size: 248 702 1496 2341
Fragments arranged by size:
2341 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1589 1976 2497 3175 3586 3733 4007 1589
Size: 387 521 678 411 147 274 2369
Fragments arranged by size:
2369 678 521 411 387 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 199 196
Size: 3 4784
BseYI C'CCAG_C
Cuts at: 1449 1614 2212 2970 3635 4043 1449
Size: 165 598 758 665 408 2193
Fragments arranged by size:
2193 758 665 598 408 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1715 1715
Size: 4787
BsiEI CG_ry'CG
Cuts at: 104 2329 2375 3605 104
Size: 2225 46 1230 1286
Fragments arranged by size:
2225 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 175 1576 1801 2579 2769 3629 175
Size: 1401 225 778 190 860 1333
Fragments arranged by size:
1401 1333 860 778 225 190
Bsp1286I G_dGCh'C
Cuts at: 175 1576 1801 2412 2505 2579 2769 2831 3629
Size: 1401 225 611 93 74 190 62 798
Cuts at: 3629 175
Size: 1333
Fragments arranged by size:
1401 1333 798 611 225 190 93 74
62
BspHI T'CATG_A
Cuts at: 3219 3219
Size: 4787
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1078 1289 1367 1837 2051 2353 2734 1078
Size: 211 78 470 214 302 381 3131
Fragments arranged by size:
3131 470 381 302 214 211 78
BsrBI CCG'CTC
Cuts at: 50 3079 3133 4617 4782 50
Size: 3029 54 1484 165 55
Fragments arranged by size:
3029 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 713 2699 713
Size: 1986 2801
BsrFI r'CCGG_y
Cuts at: 1349 2785 2966 1349
Size: 1436 181 3170
Fragments arranged by size:
3170 1436 181
BsrGI T'GTAC_A
Cuts at: 380 1679 380
Size: 1299 3488
BssSI C'ACGA_G
Cuts at: 3058 3766 3058
Size: 708 4079
BstAPI GCAn_nnn'nTGC
Cuts at: 2164 2164
Size: 4787
BstBI TT'CG_AA
Cuts at: 3148 3148
Size: 4787
BstXI CCAn_nnnn'nTGG
Cuts at: 813 813
Size: 4787
BstYI r'GATC_y
Cuts at: 1 272 451 1109 1812 2279 2302 2637 2883
Size: 271 179 658 703 467 23 335 246
Cuts at: 2883 3189 3201 3287 4339 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 703 658 467 449 335 306 271
246 179 86 23 12
BtgI C'CryG_G
Cuts at: 107 1184 107
Size: 1077 3710
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2737 2918 3081 2737
Size: 181 163 4443
Fragments arranged by size:
4443 181 163
BtsI GCAGTG_nn'
Cuts at: 536 2030 536
Size: 1494 3293
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 579 614 1443 1478 579
Size: 35 829 35 3888
Fragments arranged by size:
3888 829 35 35
DraI TTT'AAA
Cuts at: 1786 1786
Size: 4787
DraIII CAC_nnn'GTG
Cuts at: 1571 1571
Size: 4787
DrdI GACnn_nn'nnGTC
Cuts at: 2493 3837 2493
Size: 1344 3443
EaeI y'GGCC_r
Cuts at: 110 2372 2546 2937 2964 4309 110
Size: 2262 174 391 27 1345 588
Fragments arranged by size:
2262 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 2372 2372
Size: 4787
EarI CTCTTCn'nnn_
Cuts at: 173 1217 2810 3020 173
Size: 1044 1593 210 1940
Fragments arranged by size:
1940 1593 1044 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2995 3737 3883 4597 2995
Size: 742 146 714 3185
Fragments arranged by size:
3185 742 714 146
EcoICRI GAG'CTC
Cuts at: 173 1799 173
Size: 1626 3161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 652 661 745 783 1619 2612 3044 3397
Size: 305 9 84 38 836 993 432 353
Cuts at: 3397 4059 347
Size: 662 1075
Fragments arranged by size:
1075 993 836 662 432 353 305 84
38 9
EcoO109I rG'GnC_Cy
Cuts at: 1926 1926
Size: 4787
EcoRI G'AATT_C
Cuts at: 473 982 1791 473
Size: 509 809 3469
Fragments arranged by size:
3469 809 509
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2806 2806
Size: 4787
FspI TGC'GCA
Cuts at: 2568 2568
Size: 4787
HaeII r_GCGC'y
Cuts at: 2221 2469 3699 4470 2221
Size: 248 1230 771 2538
Fragments arranged by size:
2538 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 531 553 563 585 2629 2659 2661 2691 531
Size: 22 10 22 2044 30 2 30 2627
Fragments arranged by size:
2627 2044 30 30 22 22 10 2
HincII GTy'rAC
Cuts at: 572 1826 572
Size: 1254 3533
HindIII A'AGCT_T
Cuts at: 1684 1727 1684
Size: 43 4744
KasI G'GCGC_C
Cuts at: 2217 2465 2217
Size: 248 4539
KpnI G_GTAC'C
Cuts at: 1807 1807
Size: 4787
MluI A'CGCG_T
Cuts at: 4220 4220
Size: 4787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 387 1349 1727 3546 3730 387
Size: 962 378 1819 184 1444
Fragments arranged by size:
1819 1444 962 378 184
MscI TGG'CCA
Cuts at: 112 2548 112
Size: 2436 2351
MslI CAynn'nnrTG
Cuts at: 165 1483 2776 2903 165
Size: 1318 1293 127 2049
Fragments arranged by size:
2049 1318 1293 127
MspA1I CmG'CkG
Cuts at: 1898 2212 2572 3356 3601 1898
Size: 314 360 784 245 3084
Fragments arranged by size:
3084 784 360 314 245
NaeI GCC'GGC
Cuts at: 1351 2968 1351
Size: 1617 3170
NarI GG'CG_CC
Cuts at: 2218 2466 2218
Size: 248 4539
NcoI C'CATG_G
Cuts at: 107 107
Size: 4787
NdeI CA'TA_TG
Cuts at: 162 786 1482 162
Size: 624 696 3467
Fragments arranged by size:
3467 696 624
NgoMIV G'CCGG_C
Cuts at: 1349 2966 1349
Size: 1617 3170
NheI G'CTAG_C
Cuts at: 1528 1528
Size: 4787
NspI r_CATG'y
Cuts at: 381 1840 2340 3943 381
Size: 1459 500 1603 1225
Fragments arranged by size:
1603 1459 1225 500
PciI A'CATG_T
Cuts at: 377 3939 377
Size: 3562 1225
PflMI CCAn_nnn'nTGG
Cuts at: 430 430
Size: 4787
PfoI T'CCnGG_A
Cuts at: 653 653
Size: 4787
PmeI GTTT'AAAC
Cuts at: 1786 1786
Size: 4787
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 4755
PstI C_TGCA'G
Cuts at: 1364 1644 1834 2519 1364
Size: 280 190 685 3632
Fragments arranged by size:
3632 685 280 190
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4787
PvuII CAG'CTG
Cuts at: 2212 2572 2212
Size: 360 4427
RsrII CG'GwC_CG
Cuts at: 2982 2982
Size: 4787
SacI G_AGCT'C
Cuts at: 175 1801 175
Size: 1626 3161
SalI G'TCGA_C
Cuts at: 1824 1824
Size: 4787
SapI GCTCTTCn'nnn_
Cuts at: 2810 3020 2810
Size: 210 4577
SbfI CC_TGCA'GG
Cuts at: 1834 1834
Size: 4787
ScaI AGT'ACT
Cuts at: 1090 4304 1090
Size: 3214 1573
SexAI A'CCwGG_T
Cuts at: 1631 1631
Size: 4787
SfcI C'TryA_G
Cuts at: 33 856 1360 1640 1830 2515 3483 3674 33
Size: 823 504 280 190 685 968 191 1146
Fragments arranged by size:
1146 968 823 685 504 280 191 190
SfoI GGC'GCC
Cuts at: 2219 2467 2219
Size: 248 4539
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4787
SmaI CCC'GGG
Cuts at: 1809 1809
Size: 4787
SmlI C'TyrA_G
Cuts at: 826 1288 1942 2857 3571 3833 826
Size: 462 654 915 714 262 1780
Fragments arranged by size:
1780 915 714 654 462 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4787
SphI G_CATG'C
Cuts at: 1840 2340 1840
Size: 500 4287
StyI C'CwwG_G
Cuts at: 107 1012 1196 1921 2275 107
Size: 905 184 725 354 2619
Fragments arranged by size:
2619 905 725 354 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1321 2370 2503 2962 3171 1321
Size: 1049 133 459 209 2937
Fragments arranged by size:
2937 1049 459 209 133
TatI w'GTAC_w
Cuts at: 380 1088 1663 1679 4302 380
Size: 708 575 16 2623 865
Fragments arranged by size:
2623 865 708 575 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 865 897 4304 4336 865
Size: 32 3407 32 1316
Fragments arranged by size:
3407 1316 32 32
Tth111I GACn'n_nGTC
Cuts at: 2584 2584
Size: 4787
XbaI T'CTAG_A
Cuts at: 1818 1818
Size: 4787
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2282 2282
Size: 4787
XmaI C'CCGG_G
Cuts at: 1807 1807
Size: 4787
XmnI GAAnn'nnTTC
Cuts at: 1309 1309
Size: 4787
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NspI PciI PflMI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AhdI AlfI ApaI AscI AvrII
BbsI BbvCI BfrBI BglI BmgBI BsaXI BsiWI BsmI
BsmBI BspEI BssHII BstEII BstZ17I Bsu36I ClaI EcoNI
EcoRV FseI FspAI HpaI MfeI NotI NruI NsiI
PacI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI