Restriction map for
pF1KB9459
(Circular) (Six-base) MAPSORT of: pF1KB9459.seq Check: 6548 from: 1 to: 6785
pF1KB9459 6785 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2508 2508
Size: 6785
AccI GT'mk_AC
Cuts at: 1841 3823 1841
Size: 1982 4803
Acc65I G'GTAC_C
Cuts at: 3801 3801
Size: 6785
AclI AA'CG_TT
Cuts at: 6625 6625
Size: 6785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1352 1447 2033 2374 2834 3103 3130 4610 5042
Size: 95 586 341 460 269 27 1480 432
Cuts at: 5042 5395 6057 1352
Size: 353 662 2080
Fragments arranged by size:
2080 1480 662 586 460 432 353 341
269 95 27
AflIII A'CryG_T
Cuts at: 1604 1611 4137 5937 6218 1604
Size: 7 2526 1800 281 2171
Fragments arranged by size:
2526 2171 1800 281 7
AleI CACnn'nnGTG
Cuts at: 722 1822 2067 3721 722
Size: 1100 245 1654 3786
Fragments arranged by size:
3786 1654 1100 245
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1516 1516
Size: 6785
AlwNI CAG_nnn'CTG
Cuts at: 300 3024 4090 5528 300
Size: 2724 1066 1438 1557
Fragments arranged by size:
2724 1557 1438 1066
ApaI G_GGCC'C
Cuts at: 3506 3506
Size: 6785
ApaLI G'TGCA_C
Cuts at: 228 3403 5623 228
Size: 3175 2220 1390
Fragments arranged by size:
3175 2220 1390
ApoI r'AATT_y
Cuts at: 118 1900 3169 3190 3789 6138 6394 118
Size: 1782 1269 21 599 2349 256 509
Fragments arranged by size:
2349 1782 1269 599 509 256 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 6785
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6785
AvaI C'yCGr_G
Cuts at: 1868 2999 3499 3805 1868
Size: 1131 500 306 4848
Fragments arranged by size:
4848 1131 500 306
BamHI G'GATC_C
Cuts at: 176 2348 3810 176
Size: 2172 1462 3151
Fragments arranged by size:
3151 2172 1462
BanI G'GyrC_C
Cuts at: 1455 2566 3485 3801 4215 4463 4498 1455
Size: 1111 919 316 414 248 35 3742
Fragments arranged by size:
3742 1111 919 414 316 248 35
BanII G_rGCy'C
Cuts at: 152 2771 3506 3799 4829 152
Size: 2619 735 293 1030 2108
Fragments arranged by size:
2619 2108 1030 735 293
BbeI G_GCGC'C
Cuts at: 3489 4219 4467 3489
Size: 730 248 5807
Fragments arranged by size:
5807 730 248
BbsI GAAGACnn'nnnn_
Cuts at: 554 867 554
Size: 313 6472
BbvCI CC'TCA_GC
Cuts at: 901 901
Size: 6785
BciVI GTATCCnnnnn_n'
Cuts at: 2514 2556 4683 5739 6785 2514
Size: 42 2127 1056 1046 2514
Fragments arranged by size:
2514 2127 1056 1046 42
BclI T'GATC_A
Cuts at: 447 1415 1916 2892 4305 447
Size: 968 501 976 1413 2927
Fragments arranged by size:
2927 1413 976 968 501
BfrBI ATG'CAT
Cuts at: 1404 1404
Size: 6785
BglII A'GATC_T
Cuts at: 797 1472 2760 2861 4300 6337 797
Size: 675 1288 101 1439 2037 1245
Fragments arranged by size:
2037 1439 1288 1245 675 101
BlpI GC'TnA_GC
Cuts at: 151 3897 151
Size: 3746 3039
Bme1580I G_kGCm'C
Cuts at: 232 383 509 1460 2834 3407 3506 4410 4503
Size: 151 126 951 1374 573 99 904 93
Cuts at: 4503 5627 232
Size: 1124 1390
Fragments arranged by size:
1390 1374 1124 951 904 573 151 126
99 93
BmrI ACTGGGnnnn_n'
Cuts at: 671 744 962 1781 3997 4078 4413 6557 671
Size: 73 218 819 2216 81 335 2144 899
Fragments arranged by size:
2216 2144 899 819 335 218 81 73
BmtI G_CTAG'C
Cuts at: 1259 1259
Size: 6785
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5152 5152
Size: 6785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 1978 2086 2476 2785 332
Size: 1646 108 390 309 4332
Fragments arranged by size:
4332 1646 390 309 108
Bpu10I CC'TnA_GC
Cuts at: 901 2777 901
Size: 1876 4909
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3436 3961 4840 5554 5852 3436
Size: 525 879 714 298 4369
Fragments arranged by size:
4369 879 714 525 298
BsaI GGTCTCn'nnnn_
Cuts at: 118 968 6489 118
Size: 850 5521 414
Fragments arranged by size:
5521 850 414
BsaAI yAC'GTr
Cuts at: 4138 4138
Size: 6785
BsaBI GATnn'nnATC
Cuts at: 6 4299 6336 6
Size: 4293 2037 455
Fragments arranged by size:
4293 2037 455
BsaHI Gr'CG_yC
Cuts at: 3486 4216 4464 5166 6662 3486
Size: 730 248 702 1496 3609
Fragments arranged by size:
3609 1496 730 702 248
BsaWI w'CCGG_w
Cuts at: 3974 4495 5173 5584 5731 6005 3974
Size: 521 678 411 147 274 4754
Fragments arranged by size:
4754 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 329 2606 3190 329
Size: 2277 584 3924
Fragments arranged by size:
3924 2277 584
BseYI C'CCAG_C
Cuts at: 2126 2136 2639 2744 3257 4210 4968 5633 6041
Size: 10 503 105 513 953 758 665 408
Cuts at: 6041 2126
Size: 2870
Fragments arranged by size:
2870 953 758 665 513 503 408 105
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1927 2486 1927
Size: 559 6226
BsiEI CG_ry'CG
Cuts at: 104 723 4327 4373 5603 104
Size: 619 3604 46 1230 1286
Fragments arranged by size:
3604 1286 1230 619 46
BsiHKAI G_wGCw'C
Cuts at: 232 531 2598 2771 3407 3799 4577 4767 5627
Size: 299 2067 173 636 392 778 190 860
Cuts at: 5627 232
Size: 1390
Fragments arranged by size:
2067 1390 860 778 636 392 299 190
173
BsmI GAATG_Cn'
Cuts at: 1404 1962 2316 1404
Size: 558 354 5873
Fragments arranged by size:
5873 558 354
BsmBI CGTCTCn'nnnn_
Cuts at: 312 312
Size: 6785
Bsp1286I G_dGCh'C
Cuts at: 152 232 383 509 531 1460 2598 2771 2834
Size: 80 151 126 22 929 1138 173 63
Cuts at: 2834 3407 3506 3799 4410 4503 4577 4767 4829
Size: 573 99 293 611 93 74 190 62
Cuts at: 4829 5627 152
Size: 798 1310
Fragments arranged by size:
1310 1138 929 798 611 573 293 190
173 151 126 99 93 80 74 63
62 22
BspHI T'CATG_A
Cuts at: 414 1680 2937 5217 414
Size: 1266 1257 2280 1982
Fragments arranged by size:
2280 1982 1266 1257
BspMI ACCTGCnnnn'nnnn_
Cuts at: 847 2508 3401 3835 4049 4351 4732 847
Size: 1661 893 434 214 302 381 2900
Fragments arranged by size:
2900 1661 893 434 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5077 5131 6615 6780 50
Size: 5027 54 1484 165 55
Fragments arranged by size:
5027 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 370 2570 4697 370
Size: 2200 2127 2458
Fragments arranged by size:
2458 2200 2127
BsrFI r'CCGG_y
Cuts at: 4783 4964 4783
Size: 181 6604
BsrGI T'GTAC_A
Cuts at: 1246 1286 1246
Size: 40 6745
BssHII G'CGCG_C
Cuts at: 134 134
Size: 6785
BssSI C'ACGA_G
Cuts at: 5056 5764 5056
Size: 708 6077
BstAPI GCAn_nnn'nTGC
Cuts at: 1231 2602 3402 4162 1231
Size: 1371 800 760 3854
Fragments arranged by size:
3854 1371 800 760
BstBI TT'CG_AA
Cuts at: 1791 2915 5146 1791
Size: 1124 2231 3430
Fragments arranged by size:
3430 2231 1124
BstEII G'GTnAC_C
Cuts at: 3714 3714
Size: 6785
BstXI CCAn_nnnn'nTGG
Cuts at: 1042 2109 1042
Size: 1067 5718
BstYI r'GATC_y
Cuts at: 1 176 797 1472 2348 2760 2861 3810 4277
Size: 175 621 675 876 412 101 949 467
Cuts at: 4277 4300 4635 4881 5187 5199 5285 6337 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 949 876 675 621 467 449 412
335 306 246 175 101 86 23 12
BstZ17I GTA'TAC
Cuts at: 1842 1842
Size: 6785
Bsu36I CC'TnA_GG
Cuts at: 1347 3512 1347
Size: 2165 4620
BtgI C'CryG_G
Cuts at: 1779 2400 3302 3639 1779
Size: 621 902 337 4925
Fragments arranged by size:
4925 902 621 337
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4735 4916 5079 4735
Size: 181 163 6441
Fragments arranged by size:
6441 181 163
BtsI GCAGTG_nn'
Cuts at: 698 2020 4028 698
Size: 1322 2008 3455
Fragments arranged by size:
3455 2008 1322
DraI TTT'AAA
Cuts at: 3784 3784
Size: 6785
DraIII CAC_nnn'GTG
Cuts at: 1950 3670 1950
Size: 1720 5065
DrdI GACnn_nn'nnGTC
Cuts at: 4491 5835 4491
Size: 1344 5441
EaeI y'GGCC_r
Cuts at: 1075 1971 2298 4370 4544 4935 4962 6307 1075
Size: 896 327 2072 174 391 27 1345 1553
Fragments arranged by size:
2072 1553 1345 896 391 327 174 27
EagI C'GGCC_G
Cuts at: 4370 4370
Size: 6785
EarI CTCTTCn'nnn_
Cuts at: 4808 5018 4808
Size: 210 6575
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4993 5735 5881 6595 4993
Size: 742 146 714 5183
Fragments arranged by size:
5183 742 714 146
EcoICRI GAG'CTC
Cuts at: 2769 3797 2769
Size: 1028 5757
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 1352 1447 1978 2033 2086 2374 2476 2785
Size: 1020 95 531 55 53 288 102 309
Cuts at: 2785 2834 3103 3130 4610 5042 5395 6057 332
Size: 49 269 27 1480 432 353 662 1060
Fragments arranged by size:
1480 1060 1020 662 531 432 353 309
288 269 102 95 55 53 49 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2155 2155
Size: 6785
EcoO109I rG'GnC_Cy
Cuts at: 993 1706 3674 3924 993
Size: 713 1968 250 3854
Fragments arranged by size:
3854 1968 713 250
EcoRI G'AATT_C
Cuts at: 3789 3789
Size: 6785
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4804 4804
Size: 6785
FspI TGC'GCA
Cuts at: 4566 4566
Size: 6785
HaeII r_GCGC'y
Cuts at: 360 3489 4219 4467 5697 6468 360
Size: 3129 730 248 1230 771 677
Fragments arranged by size:
3129 1230 771 730 677 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 161 193 216 248 513 545 1294 1326 1407
Size: 32 23 32 265 32 749 32 81
Cuts at: 1407 1439 1501 1533 3750 3782 4627 4657 4659
Size: 32 62 32 2217 32 845 30 2
Cuts at: 4659 4689 161
Size: 30 2257
Fragments arranged by size:
2257 2217 845 749 265 81 62 32
32 32 32 32 32 32 30 30
23 2
HincII GTy'rAC
Cuts at: 113 500 3824 113
Size: 387 3324 3074
Fragments arranged by size:
3324 3074 387
HindIII A'AGCT_T
Cuts at: 349 3112 349
Size: 2763 4022
HpaI GTT'AAC
Cuts at: 113 500 113
Size: 387 6398
KasI G'GCGC_C
Cuts at: 3485 4215 4463 3485
Size: 730 248 5807
Fragments arranged by size:
5807 730 248
KpnI G_GTAC'C
Cuts at: 3805 3805
Size: 6785
MfeI C'AATT_G
Cuts at: 2526 2526
Size: 6785
MluI A'CGCG_T
Cuts at: 6218 6218
Size: 6785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2645 2878 3153 5544 5728 2645
Size: 233 275 2391 184 3702
Fragments arranged by size:
3702 2391 275 233 184
MscI TGG'CCA
Cuts at: 1973 2300 4546 1973
Size: 327 2246 4212
Fragments arranged by size:
4212 2246 327
MslI CAynn'nnrTG
Cuts at: 460 722 1040 1822 2067 3721 4774 4901 460
Size: 262 318 782 245 1654 1053 127 2344
Fragments arranged by size:
2344 1654 1053 782 318 262 245 127
MspA1I CmG'CkG
Cuts at: 1137 2392 2803 3100 3141 3469 3896 4210 4570
Size: 1255 411 297 41 328 427 314 360
Cuts at: 4570 5354 5599 1137
Size: 784 245 2323
Fragments arranged by size:
2323 1255 784 427 411 360 328 314
297 245 41
NaeI GCC'GGC
Cuts at: 4966 4966
Size: 6785
NarI GG'CG_CC
Cuts at: 3486 4216 4464 3486
Size: 730 248 5807
Fragments arranged by size:
5807 730 248
NcoI C'CATG_G
Cuts at: 2400 2400
Size: 6785
NgoMIV G'CCGG_C
Cuts at: 4964 4964
Size: 6785
NheI G'CTAG_C
Cuts at: 1255 1255
Size: 6785
NsiI A_TGCA'T
Cuts at: 1406 1406
Size: 6785
NspI r_CATG'y
Cuts at: 706 1608 1615 3594 3838 4338 5941 706
Size: 902 7 1979 244 500 1603 1550
Fragments arranged by size:
1979 1603 1550 902 500 244 7
PasI CC'CwG_GG
Cuts at: 2794 2794
Size: 6785
PciI A'CATG_T
Cuts at: 1604 1611 5937 1604
Size: 7 4326 2452
Fragments arranged by size:
4326 2452 7
PflMI CCAn_nnn'nTGG
Cuts at: 2175 3670 2175
Size: 1495 5290
PmeI GTTT'AAAC
Cuts at: 3784 3784
Size: 6785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5231 5263 5231
Size: 32 6753
PpuMI rG'GwC_Cy
Cuts at: 993 993
Size: 6785
PshAI GACnn'nnGTC
Cuts at: 2370 2370
Size: 6785
PspOMI G'GGCC_C
Cuts at: 3502 3502
Size: 6785
PstI C_TGCA'G
Cuts at: 2159 2663 3398 3712 3773 3832 4517 2159
Size: 504 735 314 61 59 685 4427
Fragments arranged by size:
4427 735 685 504 314 61 59
PvuI CG_AT'CG
Cuts at: 104 723 104
Size: 619 6166
PvuII CAG'CTG
Cuts at: 1137 2392 2803 3100 3141 3469 4210 4570 1137
Size: 1255 411 297 41 328 741 360 3352
Fragments arranged by size:
3352 1255 741 411 360 328 297 41
RsrII CG'GwC_CG
Cuts at: 4980 4980
Size: 6785
SacI G_AGCT'C
Cuts at: 2771 3799 2771
Size: 1028 5757
SalI G'TCGA_C
Cuts at: 3822 3822
Size: 6785
SapI GCTCTTCn'nnn_
Cuts at: 4808 5018 4808
Size: 210 6575
SbfI CC_TGCA'GG
Cuts at: 3832 3832
Size: 6785
ScaI AGT'ACT
Cuts at: 6302 6302
Size: 6785
SfcI C'TryA_G
Cuts at: 33 683 2155 2659 2690 2986 3394 3708 3769
Size: 650 1472 504 31 296 408 314 61
Cuts at: 3769 3828 4513 5481 5672 33
Size: 59 685 968 191 1146
Fragments arranged by size:
1472 1146 968 685 650 504 408 314
296 191 61 59 31
SfoI GGC'GCC
Cuts at: 3487 4217 4465 3487
Size: 730 248 5807
Fragments arranged by size:
5807 730 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6785
SmaI CCC'GGG
Cuts at: 1870 3807 1870
Size: 1937 4848
SmlI C'TyrA_G
Cuts at: 3451 3940 4855 5569 5831 3451
Size: 489 915 714 262 4405
Fragments arranged by size:
4405 915 714 489 262
SpeI A'CTAG_T
Cuts at: 67 184 1965 67
Size: 117 1781 4887
Fragments arranged by size:
4887 1781 117
SphI G_CATG'C
Cuts at: 3838 4338 3838
Size: 500 6285
StuI AGG'CCT
Cuts at: 1352 1352
Size: 6785
StyI C'CwwG_G
Cuts at: 172 2400 2840 3919 4273 172
Size: 2228 440 1079 354 2684
Fragments arranged by size:
2684 2228 1079 440 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1136 2314 3397 4368 4501 4960 5169 1136
Size: 1178 1083 971 133 459 209 2752
Fragments arranged by size:
2752 1178 1083 971 459 209 133
TatI w'GTAC_w
Cuts at: 705 1246 1286 3284 6300 705
Size: 541 40 1998 3016 1190
Fragments arranged by size:
3016 1998 1190 541 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 420 452 1869 1901 6302 6334 420
Size: 32 1417 32 4401 32 871
Fragments arranged by size:
4401 1417 871 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4582 4582
Size: 6785
XbaI T'CTAG_A
Cuts at: 478 1263 3339 3816 478
Size: 785 2076 477 3447
Fragments arranged by size:
3447 2076 785 477
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 549 2861 3513 4280 549
Size: 2312 652 767 3054
Fragments arranged by size:
3054 2312 767 652
XmaI C'CCGG_G
Cuts at: 1868 3805 1868
Size: 1937 4848
XmnI GAAnn'nnTTC
Cuts at: 2252 2252
Size: 6785
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI AscI AvrII
BaeI BcgI BglI BmgBI BsaXI BsiWI BspEI ClaI
CspCI EcoRV FseI FspAI NdeI NotI NruI PacI
PfoI PmlI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI