Restriction map for
pF1KB9458
(Circular) (Six-base) MAPSORT of: pF1KB9458.seq Check: 8038 from: 1 to: 5624
pF1KB9458 5624 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1279 2011 2274 1279
Size: 732 263 4629
Fragments arranged by size:
4629 732 263
AatII G_ACGT'C
Cuts at: 785 785
Size: 5624
AccI GT'mk_AC
Cuts at: 1424 1559 1850 2662 1424
Size: 135 291 812 4386
Fragments arranged by size:
4386 812 291 135
Acc65I G'GTAC_C
Cuts at: 2640 2640
Size: 5624
AclI AA'CG_TT
Cuts at: 5464 5464
Size: 5624
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 1093 1677 2313 2587 3449 3881 4234 4896
Size: 411 584 636 274 862 432 353 662
Cuts at: 4896 682
Size: 1410
Fragments arranged by size:
1410 862 662 636 584 432 411 353
274
AfeI AGC'GCT
Cuts at: 494 494
Size: 5624
AflII C'TTAA_G
Cuts at: 2242 2242
Size: 5624
AflIII A'CryG_T
Cuts at: 2976 4776 5057 2976
Size: 1800 281 3543
Fragments arranged by size:
3543 1800 281
AgeI A'CCGG_T
Cuts at: 306 2318 306
Size: 2012 3612
AleI CACnn'nnGTG
Cuts at: 1681 1882 2368 1681
Size: 201 486 4937
Fragments arranged by size:
4937 486 201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 979 2296 979
Size: 1317 4307
AlwNI CAG_nnn'CTG
Cuts at: 419 556 1662 2462 2929 4367 419
Size: 137 1106 800 467 1438 1676
Fragments arranged by size:
1676 1438 1106 800 467 137
ApaI G_GGCC'C
Cuts at: 513 1232 513
Size: 719 4905
ApaLI G'TGCA_C
Cuts at: 873 1234 4462 873
Size: 361 3228 2035
Fragments arranged by size:
3228 2035 361
ApoI r'AATT_y
Cuts at: 2628 4977 5233 2628
Size: 2349 256 3019
Fragments arranged by size:
3019 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5624
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5624
AvaI C'yCGr_G
Cuts at: 501 1183 2391 2507 2530 2644 501
Size: 682 1208 116 23 114 3481
Fragments arranged by size:
3481 1208 682 116 114 23
BamHI G'GATC_C
Cuts at: 2649 2649
Size: 5624
BanI G'GyrC_C
Cuts at: 309 1193 2640 3054 3302 3337 309
Size: 884 1447 414 248 35 2596
Fragments arranged by size:
2596 1447 884 414 248 35
BanII G_rGCy'C
Cuts at: 502 513 1088 1184 1232 2638 3668 502
Size: 11 575 96 48 1406 1030 2458
Fragments arranged by size:
2458 1406 1030 575 96 48 11
BbeI G_GCGC'C
Cuts at: 3058 3306 3058
Size: 248 5376
BbsI GAAGACnn'nnnn_
Cuts at: 360 574 1618 1851 1907 2070 2393 2580 360
Size: 214 1044 233 56 163 323 187 3404
Fragments arranged by size:
3404 1044 323 233 214 187 163 56
BbvCI CC'TCA_GC
Cuts at: 2582 2582
Size: 5624
BciVI GTATCCnnnnn_n'
Cuts at: 3522 4578 5624 3522
Size: 1056 1046 3522
Fragments arranged by size:
3522 1056 1046
BclI T'GATC_A
Cuts at: 1439 3144 1439
Size: 1705 3919
BglII A'GATC_T
Cuts at: 3139 5176 3139
Size: 2037 3587
BlpI GC'TnA_GC
Cuts at: 2525 2736 2525
Size: 211 5413
Bme1580I G_kGCm'C
Cuts at: 513 877 1232 1238 3249 3342 4466 513
Size: 364 355 6 2011 93 1124 1671
Fragments arranged by size:
2011 1671 1124 364 355 93 6
BmgBI CAC'GTC
Cuts at: 1937 1937
Size: 5624
BmrI ACTGGGnnnn_n'
Cuts at: 2836 2917 3252 5396 2836
Size: 81 335 2144 3064
Fragments arranged by size:
3064 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 260 1299 3991 260
Size: 1039 2692 1893
Fragments arranged by size:
2692 1893 1039
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 107 118 441 655 1099 1147 1312 1529 1550
Size: 11 323 214 444 48 165 217 21
Cuts at: 1550 2029 2065 107
Size: 479 36 3666
Fragments arranged by size:
3666 479 444 323 217 214 165 48
36 21 11
Bpu10I CC'TnA_GC
Cuts at: 2268 2422 2582 2268
Size: 154 160 5310
Fragments arranged by size:
5310 160 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1687 2800 3679 4393 4691 1687
Size: 1113 879 714 298 2620
Fragments arranged by size:
2620 1113 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 106 1312 1820 2567 5328 106
Size: 1206 508 747 2761 402
Fragments arranged by size:
2761 1206 747 508 402
BsaAI yAC'GTr
Cuts at: 2977 2977
Size: 5624
BsaBI GATnn'nnATC
Cuts at: 6 1042 1789 3138 5175 6
Size: 1036 747 1349 2037 455
Fragments arranged by size:
2037 1349 1036 747 455
BsaHI Gr'CG_yC
Cuts at: 782 1108 2408 3055 3303 4005 5501 782
Size: 326 1300 647 248 702 1496 905
Fragments arranged by size:
1496 1300 905 702 647 326 248
BsaWI w'CCGG_w
Cuts at: 306 2318 2813 3334 4012 4423 4570 4844 306
Size: 2012 495 521 678 411 147 274 1086
Fragments arranged by size:
2012 1086 678 521 495 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 105 135 767 797 1171 1201 105
Size: 30 632 30 374 30 4528
Fragments arranged by size:
4528 632 374 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 213 1096 1230 1568 213
Size: 883 134 338 4269
Fragments arranged by size:
4269 883 338 134
BseYI C'CCAG_C
Cuts at: 145 489 495 513 885 1126 3049 3807 4472
Size: 344 6 18 372 241 1923 758 665
Cuts at: 4472 4880 145
Size: 408 889
Fragments arranged by size:
1923 889 758 665 408 372 344 241
18 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 703 952 1045 1209 583
Size: 120 249 93 164 4998
Fragments arranged by size:
4998 249 164 120 93
BsiEI CG_ry'CG
Cuts at: 104 929 1001 1603 3166 3212 4442 104
Size: 825 72 602 1563 46 1230 1286
Fragments arranged by size:
1563 1286 1230 825 602 72 46
BsiHKAI G_wGCw'C
Cuts at: 877 1088 1238 2508 2638 3416 3606 4466 877
Size: 211 150 1270 130 778 190 860 2035
Fragments arranged by size:
2035 1270 860 778 211 190 150 130
BsmBI CGTCTCn'nnnn_
Cuts at: 289 729 289
Size: 440 5184
Bsp1286I G_dGCh'C
Cuts at: 502 513 877 1088 1184 1232 1238 2508 2638
Size: 11 364 211 96 48 6 1270 130
Cuts at: 2638 3249 3342 3416 3606 3668 4466 502
Size: 611 93 74 190 62 798 1660
Fragments arranged by size:
1660 1270 798 611 364 211 190 130
96 93 74 62 48 11 6
BspHI T'CATG_A
Cuts at: 1802 4056 1802
Size: 2254 3370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 605 1279 2011 2274 2674 2888 3190 3571 605
Size: 674 732 263 400 214 302 381 2658
Fragments arranged by size:
2658 732 674 400 381 302 263 214
BsrBI CCG'CTC
Cuts at: 50 2267 3916 3970 5454 5619 50
Size: 2217 1649 54 1484 165 55
Fragments arranged by size:
2217 1649 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2138 3536 2138
Size: 1398 4226
BsrFI r'CCGG_y
Cuts at: 306 2318 3622 3803 306
Size: 2012 1304 181 2127
Fragments arranged by size:
2127 2012 1304 181
BsrGI T'GTAC_A
Cuts at: 1028 1433 1028
Size: 405 5219
BssSI C'ACGA_G
Cuts at: 225 3895 4603 225
Size: 3670 708 1246
Fragments arranged by size:
3670 1246 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3001 3001
Size: 5624
BstBI TT'CG_AA
Cuts at: 3985 3985
Size: 5624
BstEII G'GTnAC_C
Cuts at: 753 753
Size: 5624
BstXI CCAn_nnnn'nTGG
Cuts at: 885 1884 2007 2034 885
Size: 999 123 27 4475
Fragments arranged by size:
4475 999 123 27
BstYI r'GATC_y
Cuts at: 1 416 1172 1535 2649 3116 3139 3474 3720
Size: 415 756 363 1114 467 23 335 246
Cuts at: 3720 4026 4038 4124 5176 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1114 1052 756 467 449 415 363 335
306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 360 360
Size: 5624
BtgI C'CryG_G
Cuts at: 107 154 798 1451 1472 1679 1721 1770 107
Size: 47 644 653 21 207 42 49 3961
Fragments arranged by size:
3961 653 644 207 49 47 42 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 708 3574 3755 3918 708
Size: 2866 181 163 2414
Fragments arranged by size:
2866 2414 181 163
BtsI GCAGTG_nn'
Cuts at: 715 2867 715
Size: 2152 3472
DraI TTT'AAA
Cuts at: 2623 2623
Size: 5624
DraIII CAC_nnn'GTG
Cuts at: 1217 1217
Size: 5624
DrdI GACnn_nn'nnGTC
Cuts at: 1390 2584 3330 4674 1390
Size: 1194 746 1344 2340
Fragments arranged by size:
2340 1344 1194 746
EaeI y'GGCC_r
Cuts at: 157 866 926 986 998 1600 1673 3209 3383
Size: 709 60 60 12 602 73 1536 174
Cuts at: 3383 3774 3801 5146 157
Size: 391 27 1345 635
Fragments arranged by size:
1536 1345 709 635 602 391 174 73
60 60 27 12
EagI C'GGCC_G
Cuts at: 926 998 1600 3209 926
Size: 72 602 1609 3341
Fragments arranged by size:
3341 1609 602 72
EarI CTCTTCn'nnn_
Cuts at: 441 2227 3647 3857 441
Size: 1786 1420 210 2208
Fragments arranged by size:
2208 1786 1420 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3832 4574 4720 5434 3832
Size: 742 146 714 4022
Fragments arranged by size:
4022 742 714 146
EcoICRI GAG'CTC
Cuts at: 1086 2636 1086
Size: 1550 4074
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 107 118 441 655 682 1093 1099 1147 1312
Size: 11 323 214 27 411 6 48 165
Cuts at: 1312 1529 1550 1677 2029 2065 2313 2587 3449
Size: 217 21 127 352 36 248 274 862
Cuts at: 3449 3881 4234 4896 107
Size: 432 353 662 835
Fragments arranged by size:
862 835 662 432 411 353 352 323
274 248 217 214 165 127 48 36
27 21 11 6
EcoNI CCTnn'n_nnAGG
Cuts at: 560 979 1464 560
Size: 419 485 4720
Fragments arranged by size:
4720 485 419
EcoO109I rG'GnC_Cy
Cuts at: 509 510 1954 1967 2763 509
Size: 1 1444 13 796 3370
Fragments arranged by size:
3370 1444 796 13 1
EcoRI G'AATT_C
Cuts at: 2628 2628
Size: 5624
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1419 2094 3643 1419
Size: 675 1549 3400
Fragments arranged by size:
3400 1549 675
FspI TGC'GCA
Cuts at: 3405 3405
Size: 5624
HaeII r_GCGC'y
Cuts at: 496 941 3058 3306 4536 5307 496
Size: 445 2117 248 1230 771 813
Fragments arranged by size:
2117 1230 813 771 445 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 105 137 767 799 1311 1343 1525 1557 1921
Size: 32 630 32 512 32 182 32 364
Cuts at: 1921 1953 2503 2535 3466 3496 3498 3528 105
Size: 32 550 32 931 30 2 30 2201
Fragments arranged by size:
2201 931 630 550 512 364 182 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 675 2163 2663 675
Size: 1488 500 3636
Fragments arranged by size:
3636 1488 500
KasI G'GCGC_C
Cuts at: 3054 3302 3054
Size: 248 5376
KpnI G_GTAC'C
Cuts at: 2644 2644
Size: 5624
MluI A'CGCG_T
Cuts at: 5057 5057
Size: 5624
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4383 4567 4383
Size: 184 5440
MscI TGG'CCA
Cuts at: 1675 3385 1675
Size: 1710 3914
MslI CAynn'nnrTG
Cuts at: 1681 1882 2032 2368 3613 3740 1681
Size: 201 150 336 1245 127 3565
Fragments arranged by size:
3565 1245 336 201 150 127
MspA1I CmG'CkG
Cuts at: 885 1113 1126 1167 1356 1772 1882 2735 3049
Size: 228 13 41 189 416 110 853 314
Cuts at: 3049 3409 4193 4438 885
Size: 360 784 245 2071
Fragments arranged by size:
2071 853 784 416 360 314 245 228
189 110 41 13
NaeI GCC'GGC
Cuts at: 3805 3805
Size: 5624
NarI GG'CG_CC
Cuts at: 3055 3303 3055
Size: 248 5376
NcoI C'CATG_G
Cuts at: 107 798 1451 1721 107
Size: 691 653 270 4010
Fragments arranged by size:
4010 691 653 270
NgoMIV G'CCGG_C
Cuts at: 3803 3803
Size: 5624
NspI r_CATG'y
Cuts at: 1029 1341 1758 2136 2677 3177 4780 1029
Size: 312 417 378 541 500 1603 1873
Fragments arranged by size:
1873 1603 541 500 417 378 312
PciI A'CATG_T
Cuts at: 4776 4776
Size: 5624
PflMI CCAn_nnn'nTGG
Cuts at: 419 1785 419
Size: 1366 4258
PfoI T'CCnGG_A
Cuts at: 632 1175 1201 2219 632
Size: 543 26 1018 4037
Fragments arranged by size:
4037 1018 543 26
PmeI GTTT'AAAC
Cuts at: 2623 2623
Size: 5624
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4070 4102 4070
Size: 32 5592
PspOMI G'GGCC_C
Cuts at: 509 1228 509
Size: 719 4905
PstI C_TGCA'G
Cuts at: 445 911 1373 1595 1658 1966 2204 2285 2671
Size: 466 462 222 63 308 238 81 386
Cuts at: 2671 3356 445
Size: 685 2713
Fragments arranged by size:
2713 685 466 462 386 308 238 222
81 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5624
PvuII CAG'CTG
Cuts at: 885 1126 3049 3409 885
Size: 241 1923 360 3100
Fragments arranged by size:
3100 1923 360 241
RsrII CG'GwC_CG
Cuts at: 3819 3819
Size: 5624
SacI G_AGCT'C
Cuts at: 1088 2638 1088
Size: 1550 4074
SacII CC_GC'GG
Cuts at: 1773 1773
Size: 5624
SalI G'TCGA_C
Cuts at: 2661 2661
Size: 5624
SapI GCTCTTCn'nnn_
Cuts at: 441 2227 3647 3857 441
Size: 1786 1420 210 2208
Fragments arranged by size:
2208 1786 1420 210
SbfI CC_TGCA'GG
Cuts at: 1373 1966 2671 1373
Size: 593 705 4326
Fragments arranged by size:
4326 705 593
ScaI AGT'ACT
Cuts at: 5141 5141
Size: 5624
SfcI C'TryA_G
Cuts at: 33 441 907 1305 1369 1464 1591 1632 1654
Size: 408 466 398 64 95 127 41 22
Cuts at: 1654 1962 2200 2281 2667 3352 4320 4511 33
Size: 308 238 81 386 685 968 191 1146
Fragments arranged by size:
1146 968 685 466 408 398 386 308
238 191 127 95 81 64 41 22
SfoI GGC'GCC
Cuts at: 3056 3304 3056
Size: 248 5376
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5624
SgrAI Cr'CCGG_yG
Cuts at: 306 306
Size: 5624
SmaI CCC'GGG
Cuts at: 2393 2646 2393
Size: 253 5371
SmlI C'TyrA_G
Cuts at: 1702 2242 2507 2779 3694 4408 4670 1702
Size: 540 265 272 915 714 262 2656
Fragments arranged by size:
2656 915 714 540 272 265 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5624
SphI G_CATG'C
Cuts at: 2677 3177 2677
Size: 500 5124
StyI C'CwwG_G
Cuts at: 107 798 1451 1721 1739 2758 3112 107
Size: 691 653 270 18 1019 354 2619
Fragments arranged by size:
2619 1019 691 653 354 270 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2140 3207 3340 3799 4008 2140
Size: 1067 133 459 209 3756
Fragments arranged by size:
3756 1067 459 209 133
TatI w'GTAC_w
Cuts at: 1028 1433 1688 1994 5139 1028
Size: 405 255 306 3145 1513
Fragments arranged by size:
3145 1513 405 306 255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1171 1203 5141 5173 1171
Size: 32 3938 32 1622
Fragments arranged by size:
3938 1622 32 32
Tth111I GACn'n_nGTC
Cuts at: 333 3421 333
Size: 3088 2536
XbaI T'CTAG_A
Cuts at: 2655 2655
Size: 5624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 948 2217 3119 948
Size: 1269 902 3453
Fragments arranged by size:
3453 1269 902
XhoI C'TCGA_G
Cuts at: 2507 2507
Size: 5624
XmaI C'CCGG_G
Cuts at: 2391 2644 2391
Size: 253 5371
XmnI GAAnn'nnTTC
Cuts at: 2236 2260 2236
Size: 24 5600
ZraI GAC'GTC
Cuts at: 783 783
Size: 5624
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AhdI AloI AscI AvrII BaeI BcgI BfrBI BglI
BmtI BsiWI BsmI BspEI BssHII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspXI PsrI SanDI SexAI SfiI SnaBI SrfI
SspI StuI SwaI