Restriction map for
pF1KB9456
(Circular) (Six-base) MAPSORT of: pF1KB9456.seq Check: 8822 from: 1 to: 6563
pF1KB9456 6563 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 680 693 1067 3601 680
Size: 13 374 2534 3642
Fragments arranged by size:
3642 2534 374 13
Acc65I G'GTAC_C
Cuts at: 664 931 3579 664
Size: 267 2648 3648
Fragments arranged by size:
3648 2648 267
AclI AA'CG_TT
Cuts at: 6403 6403
Size: 6563
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 125 647 745 1475 1846 4388 4820 5173 5835
Size: 522 98 730 371 2542 432 353 662
Cuts at: 5835 125
Size: 853
Fragments arranged by size:
2542 853 730 662 522 432 371 353
98
AflIII A'CryG_T
Cuts at: 1846 2415 3915 5715 5996 1846
Size: 569 1500 1800 281 2413
Fragments arranged by size:
2413 1800 1500 569 281
AleI CACnn'nnGTG
Cuts at: 2479 2674 2479
Size: 195 6368
AlwNI CAG_nnn'CTG
Cuts at: 3318 3868 5306 3318
Size: 550 1438 4575
Fragments arranged by size:
4575 1438 550
ApaI G_GGCC'C
Cuts at: 892 2154 892
Size: 1262 5301
ApaLI G'TGCA_C
Cuts at: 1877 3241 5401 1877
Size: 1364 2160 3039
Fragments arranged by size:
3039 2160 1364
ApoI r'AATT_y
Cuts at: 501 3567 5916 6172 501
Size: 3066 2349 256 892
Fragments arranged by size:
3066 2349 892 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6563
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6563
AvaI C'yCGr_G
Cuts at: 230 248 878 998 1341 3278 3583 230
Size: 18 630 120 343 1937 305 3210
Fragments arranged by size:
3210 1937 630 343 305 120 18
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2949 2982 2949
Size: 33 6530
BamHI G'GATC_C
Cuts at: 333 3588 333
Size: 3255 3308
BanI G'GyrC_C
Cuts at: 664 846 931 964 1792 3579 3993 4241 4276
Size: 182 85 33 828 1787 414 248 35
Cuts at: 4276 664
Size: 2951
Fragments arranged by size:
2951 1787 828 414 248 182 85 35
33
BanII G_rGCy'C
Cuts at: 862 892 2154 2488 3577 4607 862
Size: 30 1262 334 1089 1030 2818
Fragments arranged by size:
2818 1262 1089 1030 334 30
BbeI G_GCGC'C
Cuts at: 3997 4245 3997
Size: 248 6315
BbsI GAAGACnn'nnnn_
Cuts at: 539 1193 2414 2734 2963 539
Size: 654 1221 320 229 4139
Fragments arranged by size:
4139 1221 654 320 229
BbvCI CC'TCA_GC
Cuts at: 211 211
Size: 6563
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6529
BciVI GTATCCnnnnn_n'
Cuts at: 2305 2977 4461 5517 6563 2305
Size: 672 1484 1056 1046 2305
Fragments arranged by size:
2305 1484 1056 1046 672
BclI T'GATC_A
Cuts at: 3542 4083 3542
Size: 541 6022
BfrBI ATG'CAT
Cuts at: 111 3042 3360 111
Size: 2931 318 3314
Fragments arranged by size:
3314 2931 318
BglI GCCn_nnn'nGGC
Cuts at: 2654 2654
Size: 6563
BglII A'GATC_T
Cuts at: 1621 1629 4078 6115 1621
Size: 8 2449 2037 2069
Fragments arranged by size:
2449 2069 2037 8
BlpI GC'TnA_GC
Cuts at: 2537 3675 2537
Size: 1138 5425
Bme1580I G_kGCm'C
Cuts at: 748 885 892 1881 2154 3245 4188 4281 5405
Size: 137 7 989 273 1091 943 93 1124
Cuts at: 5405 748
Size: 1906
Fragments arranged by size:
1906 1124 1091 989 943 273 137 93
7
BmrI ACTGGGnnnn_n'
Cuts at: 335 895 3775 3856 4191 6335 335
Size: 560 2880 81 335 2144 563
Fragments arranged by size:
2880 2144 563 560 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4930 4930
Size: 6563
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 773 1208 1646 2142 2391 101
Size: 672 435 438 496 249 4273
Fragments arranged by size:
4273 672 496 438 435 249
Bpu10I CC'TnA_GC
Cuts at: 211 211
Size: 6563
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 1015 3739 4618 5332 5630 572
Size: 443 2724 879 714 298 1505
Fragments arranged by size:
2724 1505 879 714 443 298
BsaI GGTCTCn'nnnn_
Cuts at: 2118 2476 6267 2118
Size: 358 3791 2414
Fragments arranged by size:
3791 2414 358
BsaAI yAC'GTr
Cuts at: 3916 3916
Size: 6563
BsaBI GATnn'nnATC
Cuts at: 6 4077 6114 6
Size: 4071 2037 455
Fragments arranged by size:
4071 2037 455
BsaHI Gr'CG_yC
Cuts at: 2700 3994 4242 4944 6440 2700
Size: 1294 248 702 1496 2823
Fragments arranged by size:
2823 1496 1294 702 248
BsaWI w'CCGG_w
Cuts at: 204 347 3333 3752 4273 4951 5362 5509 5783
Size: 143 2986 419 521 678 411 147 274
Cuts at: 5783 204
Size: 984
Fragments arranged by size:
2986 984 678 521 419 411 274 147
143
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 544 1202 2425 544
Size: 658 1223 4682
Fragments arranged by size:
4682 1223 658
BseYI C'CCAG_C
Cuts at: 853 1236 2806 3988 4746 5411 5819 853
Size: 383 1570 1182 758 665 408 1597
Fragments arranged by size:
1597 1570 1182 758 665 408 383
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2336 2943 2336
Size: 607 5956
BsiEI CG_ry'CG
Cuts at: 104 4105 4151 5381 104
Size: 4001 46 1230 1286
Fragments arranged by size:
4001 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1881 3245 3577 4355 4545 5405 1881
Size: 1364 332 778 190 860 3039
Fragments arranged by size:
3039 1364 860 778 332 190
BsiWI C'GTAC_G
Cuts at: 1116 1116
Size: 6563
BsmI GAATG_Cn'
Cuts at: 1409 1409
Size: 6563
Bsp1286I G_dGCh'C
Cuts at: 748 862 885 892 1881 2154 2488 3245 3577
Size: 114 23 7 989 273 334 757 332
Cuts at: 3577 4188 4281 4355 4545 4607 5405 748
Size: 611 93 74 190 62 798 1906
Fragments arranged by size:
1906 989 798 757 611 334 332 273
190 114 93 74 62 23 7
BspEI T'CCGG_A
Cuts at: 204 3333 204
Size: 3129 3434
BspHI T'CATG_A
Cuts at: 4995 4995
Size: 6563
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3326 3613 3827 4129 4510 3326
Size: 287 214 302 381 5379
Fragments arranged by size:
5379 381 302 287 214
BsrBI CCG'CTC
Cuts at: 50 4855 4909 6393 6558 50
Size: 4805 54 1484 165 55
Fragments arranged by size:
4805 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 827 1713 1991 2012 2817 4475 827
Size: 886 278 21 805 1658 2915
Fragments arranged by size:
2915 1658 886 805 278 21
BsrFI r'CCGG_y
Cuts at: 4561 4742 4561
Size: 181 6382
BsrGI T'GTAC_A
Cuts at: 796 953 796
Size: 157 6406
BssSI C'ACGA_G
Cuts at: 4834 5542 4834
Size: 708 5855
BstAPI GCAn_nnn'nTGC
Cuts at: 325 3940 325
Size: 3615 2948
BstBI TT'CG_AA
Cuts at: 2109 4924 2109
Size: 2815 3748
BstEII G'GTnAC_C
Cuts at: 1240 1240
Size: 6563
BstXI CCAn_nnnn'nTGG
Cuts at: 135 315 1814 2644 2814 135
Size: 180 1499 830 170 3884
Fragments arranged by size:
3884 1499 830 180 170
BstYI r'GATC_y
Cuts at: 1 333 1621 1629 2025 2933 3588 4055 4078
Size: 332 1288 8 396 908 655 467 23
Cuts at: 4078 4413 4659 4965 4977 5063 6115 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1288 1052 908 655 467 449 396 335
332 306 246 86 23 12 8
Bsu36I CC'TnA_GG
Cuts at: 3452 3452
Size: 6563
BtgI C'CryG_G
Cuts at: 1813 2225 2298 1813
Size: 412 73 6078
Fragments arranged by size:
6078 412 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4513 4694 4857 4513
Size: 181 163 6219
Fragments arranged by size:
6219 181 163
BtsI GCAGTG_nn'
Cuts at: 2773 3374 3540 3806 2773
Size: 601 166 266 5530
Fragments arranged by size:
5530 601 266 166
ClaI AT'CG_AT
Cuts at: 173 173
Size: 6563
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1674 1709 1674
Size: 35 6528
DraI TTT'AAA
Cuts at: 3562 3562
Size: 6563
DraIII CAC_nnn'GTG
Cuts at: 343 2910 343
Size: 2567 3996
DrdI GACnn_nn'nnGTC
Cuts at: 2416 4269 5613 2416
Size: 1853 1344 3366
Fragments arranged by size:
3366 1853 1344
EaeI y'GGCC_r
Cuts at: 566 1175 1804 1938 2600 4148 4322 4713 4740
Size: 609 629 134 662 1548 174 391 27
Cuts at: 4740 6085 566
Size: 1345 1044
Fragments arranged by size:
1548 1345 1044 662 629 609 391 174
134 27
EagI C'GGCC_G
Cuts at: 4148 4148
Size: 6563
EarI CTCTTCn'nnn_
Cuts at: 602 3209 4586 4796 602
Size: 2607 1377 210 2369
Fragments arranged by size:
2607 2369 1377 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3034 3369 4771 5513 5659 6373 3034
Size: 335 1402 742 146 714 3224
Fragments arranged by size:
3224 1402 742 714 335 146
EcoICRI GAG'CTC
Cuts at: 3575 3575
Size: 6563
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 101 125 647 745 773 1208 1475 1646 1846
Size: 24 522 98 28 435 267 171 200
Cuts at: 1846 2142 2391 4388 4820 5173 5835 101
Size: 296 249 1997 432 353 662 829
Fragments arranged by size:
1997 829 662 522 435 432 353 296
267 249 200 171 98 28 24
EcoNI CCTnn'n_nnAGG
Cuts at: 1465 2560 1465
Size: 1095 5468
EcoO109I rG'GnC_Cy
Cuts at: 378 2151 2350 2982 3098 3315 3702 378
Size: 1773 199 632 116 217 387 3239
Fragments arranged by size:
3239 1773 632 387 217 199 116
EcoRI G'AATT_C
Cuts at: 3567 3567
Size: 6563
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4582 4582
Size: 6563
FspI TGC'GCA
Cuts at: 4344 4344
Size: 6563
HaeII r_GCGC'y
Cuts at: 3997 4245 5475 6246 3997
Size: 248 1230 771 4314
Fragments arranged by size:
4314 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 199 231 992 1024 1261 1293 1463 1495 2163
Size: 32 761 32 237 32 170 32 668
Cuts at: 2163 2195 3157 3189 4405 4435 4437 4467 199
Size: 32 962 32 1216 30 2 30 2295
Fragments arranged by size:
2295 1216 962 761 668 237 170 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 806 1545 3393 3602 806
Size: 739 1848 209 3767
Fragments arranged by size:
3767 1848 739 209
HindIII A'AGCT_T
Cuts at: 2525 2525
Size: 6563
HpaI GTT'AAC
Cuts at: 3393 3393
Size: 6563
KasI G'GCGC_C
Cuts at: 3993 4241 3993
Size: 248 6315
KpnI G_GTAC'C
Cuts at: 668 935 3583 668
Size: 267 2648 3648
Fragments arranged by size:
3648 2648 267
MfeI C'AATT_G
Cuts at: 773 3154 773
Size: 2381 4182
MluI A'CGCG_T
Cuts at: 5996 5996
Size: 6563
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5322 5506 5322
Size: 184 6379
MscI TGG'CCA
Cuts at: 568 1177 1806 1940 4324 568
Size: 609 629 134 2384 2807
Fragments arranged by size:
2807 2384 629 609 134
MslI CAynn'nnrTG
Cuts at: 133 1019 1532 1812 2167 2479 2674 2842 3047
Size: 886 513 280 355 312 195 168 205
Cuts at: 3047 3082 4552 4679 133
Size: 35 1470 127 2017
Fragments arranged by size:
2017 1470 886 513 355 312 280 205
195 168 127 35
MspA1I CmG'CkG
Cuts at: 751 853 2592 3674 3988 4348 5132 5377 751
Size: 102 1739 1082 314 360 784 245 1937
Fragments arranged by size:
1937 1739 1082 784 360 314 245 102
NaeI GCC'GGC
Cuts at: 4744 4744
Size: 6563
NarI GG'CG_CC
Cuts at: 3994 4242 3994
Size: 248 6315
NcoI C'CATG_G
Cuts at: 1813 2225 1813
Size: 412 6151
NgoMIV G'CCGG_C
Cuts at: 4742 4742
Size: 6563
NsiI A_TGCA'T
Cuts at: 113 3044 3362 113
Size: 2931 318 3314
Fragments arranged by size:
3314 2931 318
NspI r_CATG'y
Cuts at: 1062 1236 1730 1850 2090 2419 3120 3330 3616
Size: 174 494 120 240 329 701 210 286
Cuts at: 3616 4116 5719 1062
Size: 500 1603 1906
Fragments arranged by size:
1906 1603 701 500 494 329 286 240
210 174 120
PciI A'CATG_T
Cuts at: 1846 2415 5715 1846
Size: 569 3300 2694
Fragments arranged by size:
3300 2694 569
PflMI CCAn_nnn'nTGG
Cuts at: 898 2595 898
Size: 1697 4866
PfoI T'CCnGG_A
Cuts at: 1413 3311 1413
Size: 1898 4665
PmeI GTTT'AAAC
Cuts at: 3562 3562
Size: 6563
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5009 5041 5009
Size: 32 6531
PpuMI rG'GwC_Cy
Cuts at: 378 2350 2982 3098 3315 378
Size: 1972 632 116 217 3626
Fragments arranged by size:
3626 1972 632 217 116
PshAI GACnn'nnGTC
Cuts at: 200 383 200
Size: 183 6380
PspOMI G'GGCC_C
Cuts at: 888 2150 888
Size: 1262 5301
PspXI vC'TCGA_Gb
Cuts at: 1341 1341
Size: 6563
PstI C_TGCA'G
Cuts at: 3610 4295 3610
Size: 685 5878
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6563
PvuII CAG'CTG
Cuts at: 751 853 2592 3988 4348 751
Size: 102 1739 1396 360 2966
Fragments arranged by size:
2966 1739 1396 360 102
RsrII CG'GwC_CG
Cuts at: 4758 4758
Size: 6563
SacI G_AGCT'C
Cuts at: 3577 3577
Size: 6563
SalI G'TCGA_C
Cuts at: 3600 3600
Size: 6563
SapI GCTCTTCn'nnn_
Cuts at: 4586 4796 4586
Size: 210 6353
SbfI CC_TGCA'GG
Cuts at: 3610 3610
Size: 6563
ScaI AGT'ACT
Cuts at: 6080 6080
Size: 6563
SfcI C'TryA_G
Cuts at: 33 3606 4291 5259 5450 33
Size: 3573 685 968 191 1146
Fragments arranged by size:
3573 1146 968 685 191
SfoI GGC'GCC
Cuts at: 3995 4243 3995
Size: 248 6315
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6563
SmaI CCC'GGG
Cuts at: 250 880 3280 3585 250
Size: 630 2400 305 3228
Fragments arranged by size:
3228 2400 630 305
SmlI C'TyrA_G
Cuts at: 587 1030 1341 3718 4633 5347 5609 587
Size: 443 311 2377 915 714 262 1541
Fragments arranged by size:
2377 1541 915 714 443 311 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6563
SphI G_CATG'C
Cuts at: 1236 3616 4116 1236
Size: 2380 500 3683
Fragments arranged by size:
3683 2380 500
SspI AAT'ATT
Cuts at: 490 702 490
Size: 212 6351
StuI AGG'CCT
Cuts at: 2376 2376
Size: 6563
StyI C'CwwG_G
Cuts at: 960 1286 1466 1813 1904 2225 2265 2649 3697
Size: 326 180 347 91 321 40 384 1048
Cuts at: 3697 4051 960
Size: 354 3472
Fragments arranged by size:
3472 1048 384 354 347 326 321 180
91 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 354 552 664 726 1806 2130 3182 4146 4279
Size: 198 112 62 1080 324 1052 964 133
Cuts at: 4279 4738 4947 354
Size: 459 209 1970
Fragments arranged by size:
1970 1080 1052 964 459 324 209 198
133 112 62
TatI w'GTAC_w
Cuts at: 796 953 2252 3266 6078 796
Size: 157 1299 1014 2812 1281
Fragments arranged by size:
2812 1299 1281 1014 157
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6080 6112 6080
Size: 32 6531
Tth111I GACn'n_nGTC
Cuts at: 4360 4360
Size: 6563
XbaI T'CTAG_A
Cuts at: 3594 3594
Size: 6563
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 312 840 1319 1411 2223 4058 132
Size: 180 528 479 92 812 1835 2637
Fragments arranged by size:
2637 1835 812 528 479 180 92
XhoI C'TCGA_G
Cuts at: 1341 1341
Size: 6563
XmaI C'CCGG_G
Cuts at: 248 878 3278 3583 248
Size: 630 2400 305 3228
Fragments arranged by size:
3228 2400 630 305
XmnI GAAnn'nnTTC
Cuts at: 977 1535 977
Size: 558 6005
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
AscI AvrII BmgBI BmtI BsaXI BsmBI BssHII BstZ17I
EcoRV FseI FspAI NdeI NheI NotI NruI PacI
PasI PmlI PsiI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI ZraI