Restriction map for
pF1KB9449
(Circular) (Six-base) MAPSORT of: pF1KB9449.seq Check: 5486 from: 1 to: 4508
pF1KB9449 4508 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1546 1546
Size: 4508
Acc65I G'GTAC_C
Cuts at: 1524 1524
Size: 4508
AclI AA'CG_TT
Cuts at: 4348 4348
Size: 4508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1399 2333 2765 3512 3780 1399
Size: 934 432 747 268 2127
Fragments arranged by size:
2127 934 747 432 268
AflII C'TTAA_G
Cuts at: 810 810
Size: 4508
AflIII A'CryG_T
Cuts at: 1058 1860 2964 3941 1058
Size: 802 1104 977 1625
Fragments arranged by size:
1625 1104 977 802
AgeI A'CCGG_T
Cuts at: 1465 2896 1465
Size: 1431 3077
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 794 794
Size: 4508
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1065 1097 1065
Size: 32 4476
AlwNI CAG_nnn'CTG
Cuts at: 1093 1813 3380 1093
Size: 720 1567 2221
Fragments arranged by size:
2221 1567 720
ApaI G_GGCC'C
Cuts at: 905 905
Size: 4508
ApaLI G'TGCA_C
Cuts at: 3278 3278
Size: 4508
ApoI r'AATT_y
Cuts at: 1512 3861 4117 1512
Size: 2349 256 1903
Fragments arranged by size:
2349 1903 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4508
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
AvaI C'yCGr_G
Cuts at: 169 376 436 1069 1268 1413 1486 1528 169
Size: 207 60 633 199 145 73 42 3149
Fragments arranged by size:
3149 633 207 199 145 73 60 42
BamHI G'GATC_C
Cuts at: 599 1533 599
Size: 934 3574
BanI G'GyrC_C
Cuts at: 173 1449 1524 1938 2186 2221 173
Size: 1276 75 414 248 35 2460
Fragments arranged by size:
2460 1276 414 248 75 35
BanII G_rGCy'C
Cuts at: 905 1048 1267 1327 1522 2552 905
Size: 143 219 60 195 1030 2861
Fragments arranged by size:
2861 1030 219 195 143 60
BbeI G_GCGC'C
Cuts at: 177 1453 1942 2190 177
Size: 1276 489 248 2495
Fragments arranged by size:
2495 1276 489 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1114 1148 90
Size: 34 990 34 3450
Fragments arranged by size:
3450 990 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2406 3167 4508 2406
Size: 761 1341 2406
Fragments arranged by size:
2406 1341 761
BclI T'GATC_A
Cuts at: 2028 2028
Size: 4508
BglI GCCn_nnn'nGGC
Cuts at: 131 804 131
Size: 673 3835
BglII A'GATC_T
Cuts at: 2023 4060 2023
Size: 2037 2471
BlpI GC'TnA_GC
Cuts at: 1620 1620
Size: 4508
Bme1580I G_kGCm'C
Cuts at: 809 905 1185 2133 2226 3282 809
Size: 96 280 948 93 1056 2035
Fragments arranged by size:
2035 1056 948 280 96 93
BmgBI CAC'GTC
Cuts at: 249 1059 1304 249
Size: 810 245 3453
Fragments arranged by size:
3453 810 245
BmrI ACTGGGnnnn_n'
Cuts at: 1720 1801 2136 4280 1720
Size: 81 335 2144 1948
Fragments arranged by size:
2144 1948 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2875 2875
Size: 4508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1268 946
Size: 322 4186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1684 2563 3055 3353 1684
Size: 879 492 298 2839
Fragments arranged by size:
2839 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4212 4212
Size: 4508
BsaAI yAC'GTr
Cuts at: 1861 1861
Size: 4508
BsaBI GATnn'nnATC
Cuts at: 6 713 2022 4059 6
Size: 707 1309 2037 455
Fragments arranged by size:
2037 1309 707 455
BsaHI Gr'CG_yC
Cuts at: 174 1450 1939 2187 2889 4385 174
Size: 1276 489 248 702 1496 297
Fragments arranged by size:
1496 1276 702 489 297 248
BsaWI w'CCGG_w
Cuts at: 738 745 1426 1465 1697 2218 2896 3170 3317
Size: 7 681 39 232 521 678 274 147
Cuts at: 3317 3728 738
Size: 411 1518
Fragments arranged by size:
1518 681 678 521 411 274 232 147
39 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 972 1002 1282 1312 972
Size: 30 280 30 4168
Fragments arranged by size:
4168 280 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 352 1179 1445 352
Size: 827 266 3415
Fragments arranged by size:
3415 827 266
BseYI C'CCAG_C
Cuts at: 177 363 934 1086 1933 2691 3268 3764 177
Size: 186 571 152 847 758 577 496 921
Fragments arranged by size:
921 847 758 577 571 496 186 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1273 1411 1273
Size: 138 4370
BsiEI CG_ry'CG
Cuts at: 104 151 155 2050 2096 3304 104
Size: 47 4 1895 46 1208 1308
Fragments arranged by size:
1895 1308 1208 47 46 4
BsiHKAI G_wGCw'C
Cuts at: 497 652 1405 1522 2300 2490 3282 497
Size: 155 753 117 778 190 792 1723
Fragments arranged by size:
1723 792 778 753 190 155 117
BsmI GAATG_Cn'
Cuts at: 1434 1434
Size: 4508
Bsp1286I G_dGCh'C
Cuts at: 497 652 809 905 1048 1185 1267 1327 1405
Size: 155 157 96 143 137 82 60 78
Cuts at: 1405 1522 2133 2226 2300 2490 2552 3282 497
Size: 117 611 93 74 190 62 730 1723
Fragments arranged by size:
1723 730 611 190 157 155 143 137
117 96 93 82 78 74 62 60
BspEI T'CCGG_A
Cuts at: 738 745 3728 738
Size: 7 2983 1518
Fragments arranged by size:
2983 1518 7
BspHI T'CATG_A
Cuts at: 3684 3684
Size: 4508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 287 1558 1772 2074 2455 287
Size: 1271 214 302 381 2340
Fragments arranged by size:
2340 1271 381 302 214
BsrBI CCG'CTC
Cuts at: 50 342 627 2800 2854 4338 4503 50
Size: 292 285 2173 54 1484 165 55
Fragments arranged by size:
2173 1484 292 285 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2420 2420
Size: 4508
BsrFI r'CCGG_y
Cuts at: 150 1465 2506 2687 2896 150
Size: 1315 1041 181 209 1762
Fragments arranged by size:
1762 1315 1041 209 181
BssSI C'ACGA_G
Cuts at: 2779 3137 2779
Size: 358 4150
BstAPI GCAn_nnn'nTGC
Cuts at: 1885 1885
Size: 4508
BstBI TT'CG_AA
Cuts at: 2869 2869
Size: 4508
BstEII G'GTnAC_C
Cuts at: 957 957
Size: 4508
BstXI CCAn_nnnn'nTGG
Cuts at: 1128 1128
Size: 4508
BstYI r'GATC_y
Cuts at: 1 521 599 708 1533 2000 2023 2358 2604
Size: 520 78 109 825 467 23 335 246
Cuts at: 2604 3616 3702 3714 4060 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 825 520 467 449 346 335 246
109 86 78 23 12
BtgI C'CryG_G
Cuts at: 145 1362 145
Size: 1217 3291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2458 2639 2802 2458
Size: 181 163 4164
Fragments arranged by size:
4164 181 163
BtsI GCAGTG_nn'
Cuts at: 235 793 1185 1751 235
Size: 558 392 566 2992
Fragments arranged by size:
2992 566 558 392
ClaI AT'CG_AT
Cuts at: 1025 1025
Size: 4508
DraI TTT'AAA
Cuts at: 1507 1507
Size: 4508
DrdI GACnn_nn'nnGTC
Cuts at: 2214 3072 2214
Size: 858 3650
EaeI y'GGCC_r
Cuts at: 148 152 410 2093 2267 2658 2685 4030 148
Size: 4 258 1683 174 391 27 1345 626
Fragments arranged by size:
1683 1345 626 391 258 174 27 4
EagI C'GGCC_G
Cuts at: 148 152 2093 148
Size: 4 1941 2563
Fragments arranged by size:
2563 1941 4
EarI CTCTTCn'nnn_
Cuts at: 2531 2741 2531
Size: 210 4298
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 543 934 2716 3026 3172 4318 543
Size: 391 1782 310 146 1146 733
Fragments arranged by size:
1782 1146 733 391 310 146
EcoICRI GAG'CTC
Cuts at: 1520 1520
Size: 4508
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1268 1399 2333 2765 3512 3780 946
Size: 322 131 934 432 747 268 1674
Fragments arranged by size:
1674 934 747 432 322 268 131
EcoNI CCTnn'n_nnAGG
Cuts at: 205 544 205
Size: 339 4169
EcoO109I rG'GnC_Cy
Cuts at: 901 1647 901
Size: 746 3762
EcoRI G'AATT_C
Cuts at: 1512 1512
Size: 4508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1188 2527 1188
Size: 1339 3169
FseI GG_CCGG'CC
Cuts at: 154 154
Size: 4508
FspI TGC'GCA
Cuts at: 421 2289 421
Size: 1868 2640
HaeII r_GCGC'y
Cuts at: 177 1014 1453 1942 2190 3212 4191 177
Size: 837 439 489 248 1022 979 494
Fragments arranged by size:
1022 979 837 494 489 439 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 680 712 2350 2380 2382 2412 680
Size: 32 1638 30 2 30 2776
Fragments arranged by size:
2776 1638 32 30 30 2
HincII GTy'rAC
Cuts at: 1547 1547
Size: 4508
KasI G'GCGC_C
Cuts at: 173 1449 1938 2186 173
Size: 1276 489 248 2495
Fragments arranged by size:
2495 1276 489 248
KpnI G_GTAC'C
Cuts at: 1528 1528
Size: 4508
MluI A'CGCG_T
Cuts at: 3941 3941
Size: 4508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 524 3179 3363 524
Size: 2655 184 1669
Fragments arranged by size:
2655 1669 184
MscI TGG'CCA
Cuts at: 412 2269 412
Size: 1857 2651
MslI CAynn'nnrTG
Cuts at: 2497 2624 2497
Size: 127 4381
MspA1I CmG'CkG
Cuts at: 147 1093 1112 1233 1619 1933 2293 3306 3551
Size: 946 19 121 386 314 360 1013 245
Cuts at: 3551 147
Size: 1104
Fragments arranged by size:
1104 1013 946 386 360 314 245 121
19
NaeI GCC'GGC
Cuts at: 152 2689 152
Size: 2537 1971
NarI GG'CG_CC
Cuts at: 174 1450 1939 2187 174
Size: 1276 489 248 2495
Fragments arranged by size:
2495 1276 489 248
NgoMIV G'CCGG_C
Cuts at: 150 2687 150
Size: 2537 1971
NspI r_CATG'y
Cuts at: 1561 2061 2968 1561
Size: 500 907 3101
Fragments arranged by size:
3101 907 500
PasI CC'CwG_GG
Cuts at: 963 963
Size: 4508
PciI A'CATG_T
Cuts at: 2964 2964
Size: 4508
PflMI CCAn_nnn'nTGG
Cuts at: 1093 1115 1093
Size: 22 4486
PfoI T'CCnGG_A
Cuts at: 818 818
Size: 4508
PmeI GTTT'AAAC
Cuts at: 1507 1507
Size: 4508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 3647
Size: 32 4476
PspOMI G'GGCC_C
Cuts at: 901 901
Size: 4508
PstI C_TGCA'G
Cuts at: 284 1180 1555 2240 284
Size: 896 375 685 2552
Fragments arranged by size:
2552 896 685 375
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4508
PvuII CAG'CTG
Cuts at: 1093 1933 2293 1093
Size: 840 360 3308
Fragments arranged by size:
3308 840 360
RsrII CG'GwC_CG
Cuts at: 2703 2703
Size: 4508
SacI G_AGCT'C
Cuts at: 1522 1522
Size: 4508
SacII CC_GC'GG
Cuts at: 148 148
Size: 4508
SalI G'TCGA_C
Cuts at: 1545 1545
Size: 4508
SapI GCTCTTCn'nnn_
Cuts at: 2531 2741 2531
Size: 210 4298
SbfI CC_TGCA'GG
Cuts at: 284 1555 284
Size: 1271 3237
ScaI AGT'ACT
Cuts at: 301 615 4025 301
Size: 314 3410 784
Fragments arranged by size:
3410 784 314
SexAI A'CCwGG_T
Cuts at: 268 572 953 268
Size: 304 381 3823
Fragments arranged by size:
3823 381 304
SfcI C'TryA_G
Cuts at: 33 280 1176 1551 2236 3229 3420 33
Size: 247 896 375 685 993 191 1121
Fragments arranged by size:
1121 993 896 685 375 247 191
SfoI GGC'GCC
Cuts at: 175 1451 1940 2188 175
Size: 1276 489 248 2495
Fragments arranged by size:
2495 1276 489 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
SmaI CCC'GGG
Cuts at: 171 1071 1270 1488 1530 171
Size: 900 199 218 42 3149
Fragments arranged by size:
3149 900 218 199 42
SmlI C'TyrA_G
Cuts at: 810 1663 2578 3070 3332 810
Size: 853 915 492 262 1986
Fragments arranged by size:
1986 915 853 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4508
SphI G_CATG'C
Cuts at: 1561 2061 1561
Size: 500 4008
SrfI GCCC'GGGC
Cuts at: 171 171
Size: 4508
StyI C'CwwG_G
Cuts at: 1642 1996 1642
Size: 354 4154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 220 1237 2091 2224 2683 2892 220
Size: 1017 854 133 459 209 1836
Fragments arranged by size:
1836 1017 854 459 209 133
TatI w'GTAC_w
Cuts at: 185 299 613 4023 185
Size: 114 314 3410 670
Fragments arranged by size:
3410 670 314 114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 972 1004 1282 1314 4025 4057 972
Size: 32 278 32 2711 32 1423
Fragments arranged by size:
2711 1423 278 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 574 2305 574
Size: 1731 2777
XbaI T'CTAG_A
Cuts at: 1539 1539
Size: 4508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 912 2003 912
Size: 1091 3417
XmaI C'CCGG_G
Cuts at: 169 1069 1268 1486 1528 169
Size: 900 199 218 42 3149
Fragments arranged by size:
3149 900 218 199 42
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AscI AvrII BaeI
BbsI BbvCI BfrBI BmtI Bpu10I BsiWI BsmBI BsrGI
BssHII BstZ17I Bsu36I CspCI DraIII EcoRV FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PmlI PpuMI PshAI PsiI PspXI PsrI SanDI
SfiI SgrAI SnaBI SspI StuI SwaI XhoI XmnI
ZraI