Restriction map for
pF1KB9446
(Circular) (Six-base) MAPSORT of: pF1KB9446.seq Check: 8600 from: 1 to: 4082
pF1KB9446 4082 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1120 1120
Size: 4082
Acc65I G'GTAC_C
Cuts at: 796 1098 796
Size: 302 3780
AclI AA'CG_TT
Cuts at: 3922 3922
Size: 4082
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 1907 2339 3086 3354 611
Size: 1296 432 747 268 1339
Fragments arranged by size:
1339 1296 747 432 268
AflIII A'CryG_T
Cuts at: 1434 2538 3515 1434
Size: 1104 977 2001
Fragments arranged by size:
2001 1104 977
AgeI A'CCGG_T
Cuts at: 2470 2470
Size: 4082
AhdI GACnn_n'nnGTC
Cuts at: 404 404
Size: 4082
AlwNI CAG_nnn'CTG
Cuts at: 1387 2954 1387
Size: 1567 2515
ApaI G_GGCC'C
Cuts at: 522 522
Size: 4082
ApaLI G'TGCA_C
Cuts at: 2852 2852
Size: 4082
ApoI r'AATT_y
Cuts at: 136 814 839 1008 1086 3435 3691 136
Size: 678 25 169 78 2349 256 527
Fragments arranged by size:
2349 678 527 256 169 78 25
AseI AT'TA_AT
Cuts at: 20 20
Size: 4082
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
AvaI C'yCGr_G
Cuts at: 521 1102 521
Size: 581 3501
BamHI G'GATC_C
Cuts at: 1107 1107
Size: 4082
BanI G'GyrC_C
Cuts at: 111 796 1098 1512 1760 1795 111
Size: 685 302 414 248 35 2398
Fragments arranged by size:
2398 685 414 302 248 35
BanII G_rGCy'C
Cuts at: 522 1096 2126 522
Size: 574 1030 2478
Fragments arranged by size:
2478 1030 574
BbeI G_GCGC'C
Cuts at: 115 1516 1764 115
Size: 1401 248 2433
Fragments arranged by size:
2433 1401 248
BbsI GAAGACnn'nnnn_
Cuts at: 403 403
Size: 4082
BbvCI CC'TCA_GC
Cuts at: 497 497
Size: 4082
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 143 177 143
Size: 34 4048
BciVI GTATCCnnnnn_n'
Cuts at: 1980 2741 4082 1980
Size: 761 1341 1980
Fragments arranged by size:
1980 1341 761
BclI T'GATC_A
Cuts at: 1602 1602
Size: 4082
BglII A'GATC_T
Cuts at: 1597 3634 1597
Size: 2037 2045
BlpI GC'TnA_GC
Cuts at: 1194 1194
Size: 4082
Bme1580I G_kGCm'C
Cuts at: 522 1707 1800 2856 522
Size: 1185 93 1056 1748
Fragments arranged by size:
1748 1185 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 630 1294 1375 1710 3854 630
Size: 664 81 335 2144 858
Fragments arranged by size:
2144 858 664 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 4082
Bpu10I CC'TnA_GC
Cuts at: 497 497
Size: 4082
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 1258 2137 2629 2927 709
Size: 549 879 492 298 1864
Fragments arranged by size:
1864 879 549 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 484 816 3786 484
Size: 332 2970 780
Fragments arranged by size:
2970 780 332
BsaAI yAC'GTr
Cuts at: 303 1435 303
Size: 1132 2950
BsaBI GATnn'nnATC
Cuts at: 6 1596 3633 6
Size: 1590 2037 455
Fragments arranged by size:
2037 1590 455
BsaHI Gr'CG_yC
Cuts at: 112 1513 1761 2463 3959 112
Size: 1401 248 702 1496 235
Fragments arranged by size:
1496 1401 702 248 235
BsaWI w'CCGG_w
Cuts at: 279 1271 1792 2470 2744 2891 3302 279
Size: 992 521 678 274 147 411 1059
Fragments arranged by size:
1059 992 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 494 1040 494
Size: 546 3536
BseYI C'CCAG_C
Cuts at: 800 1507 2265 2842 3338 800
Size: 707 758 577 496 1544
Fragments arranged by size:
1544 758 707 577 496
BsiEI CG_ry'CG
Cuts at: 104 1624 1670 2878 104
Size: 1520 46 1208 1308
Fragments arranged by size:
1520 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1096 1874 2064 2856 1096
Size: 778 190 792 2322
Fragments arranged by size:
2322 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 522 1096 1707 1800 1874 2064 2126 2856 522
Size: 574 611 93 74 190 62 730 1748
Fragments arranged by size:
1748 730 611 574 190 93 74 62
BspEI T'CCGG_A
Cuts at: 279 3302 279
Size: 3023 1059
BspHI T'CATG_A
Cuts at: 557 3258 557
Size: 2701 1381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1132 1346 1648 2029 1132
Size: 214 302 381 3185
Fragments arranged by size:
3185 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2374 2428 3912 4077 50
Size: 2324 54 1484 165 55
Fragments arranged by size:
2324 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 929 1994 929
Size: 1065 3017
BsrFI r'CCGG_y
Cuts at: 2080 2261 2470 2080
Size: 181 209 3692
Fragments arranged by size:
3692 209 181
BssSI C'ACGA_G
Cuts at: 716 2353 2711 716
Size: 1637 358 2087
Fragments arranged by size:
2087 1637 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1459 1459
Size: 4082
BstBI TT'CG_AA
Cuts at: 2443 2443
Size: 4082
BstXI CCAn_nnnn'nTGG
Cuts at: 880 880
Size: 4082
BstYI r'GATC_y
Cuts at: 1 528 1107 1574 1597 1932 2178 3190 3276
Size: 527 579 467 23 335 246 1012 86
Cuts at: 3276 3288 3634 1
Size: 12 346 449
Fragments arranged by size:
1012 579 527 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 604 107
Size: 497 3585
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2032 2213 2376 2032
Size: 181 163 3738
Fragments arranged by size:
3738 181 163
BtsI GCAGTG_nn'
Cuts at: 1325 1325
Size: 4082
DraI TTT'AAA
Cuts at: 1081 1081
Size: 4082
DrdI GACnn_nn'nnGTC
Cuts at: 1788 2646 1788
Size: 858 3224
EaeI y'GGCC_r
Cuts at: 1667 1841 2232 2259 3604 1667
Size: 174 391 27 1345 2145
Fragments arranged by size:
2145 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1667 1667
Size: 4082
EarI CTCTTCn'nnn_
Cuts at: 182 587 946 2105 2315 182
Size: 405 359 1159 210 1949
Fragments arranged by size:
1949 1159 405 359 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2290 2600 2746 3892 2290
Size: 310 146 1146 2480
Fragments arranged by size:
2480 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1094 1094
Size: 4082
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 1907 2339 3086 3354 611
Size: 1296 432 747 268 1339
Fragments arranged by size:
1339 1296 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 123 518 1221 123
Size: 395 703 2984
Fragments arranged by size:
2984 703 395
EcoRI G'AATT_C
Cuts at: 1008 1086 1008
Size: 78 4004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 4082
FspI TGC'GCA
Cuts at: 1863 1863
Size: 4082
HaeII r_GCGC'y
Cuts at: 115 889 1516 1764 2786 3765 115
Size: 774 627 248 1022 979 432
Fragments arranged by size:
1022 979 774 627 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 390 422 1924 1954 1956 1986 390
Size: 32 1502 30 2 30 2486
Fragments arranged by size:
2486 1502 32 30 30 2
HincII GTy'rAC
Cuts at: 1121 1121
Size: 4082
KasI G'GCGC_C
Cuts at: 111 1512 1760 111
Size: 1401 248 2433
Fragments arranged by size:
2433 1401 248
KpnI G_GTAC'C
Cuts at: 800 1102 800
Size: 302 3780
MluI A'CGCG_T
Cuts at: 3515 3515
Size: 4082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 232 2753 2937 232
Size: 2521 184 1377
Fragments arranged by size:
2521 1377 184
MscI TGG'CCA
Cuts at: 1843 1843
Size: 4082
MslI CAynn'nnrTG
Cuts at: 245 857 2071 2198 245
Size: 612 1214 127 2129
Fragments arranged by size:
2129 1214 612 127
MspA1I CmG'CkG
Cuts at: 1193 1507 1867 2880 3125 1193
Size: 314 360 1013 245 2150
Fragments arranged by size:
2150 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2263 2263
Size: 4082
NarI GG'CG_CC
Cuts at: 112 1513 1761 112
Size: 1401 248 2433
Fragments arranged by size:
2433 1401 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4082
NgoMIV G'CCGG_C
Cuts at: 2261 2261
Size: 4082
NspI r_CATG'y
Cuts at: 1135 1635 2542 1135
Size: 500 907 2675
Fragments arranged by size:
2675 907 500
PciI A'CATG_T
Cuts at: 2538 2538
Size: 4082
PfoI T'CCnGG_A
Cuts at: 140 140
Size: 4082
PmeI GTTT'AAAC
Cuts at: 1081 1081
Size: 4082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3221 3253 3221
Size: 32 4050
PpuMI rG'GwC_Cy
Cuts at: 123 123
Size: 4082
PspOMI G'GGCC_C
Cuts at: 518 518
Size: 4082
PstI C_TGCA'G
Cuts at: 1129 1814 1129
Size: 685 3397
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4082
PvuII CAG'CTG
Cuts at: 1507 1867 1507
Size: 360 3722
RsrII CG'GwC_CG
Cuts at: 2277 2277
Size: 4082
SacI G_AGCT'C
Cuts at: 1096 1096
Size: 4082
SalI G'TCGA_C
Cuts at: 1119 1119
Size: 4082
SapI GCTCTTCn'nnn_
Cuts at: 587 946 2105 2315 587
Size: 359 1159 210 2354
Fragments arranged by size:
2354 1159 359 210
SbfI CC_TGCA'GG
Cuts at: 1129 1129
Size: 4082
ScaI AGT'ACT
Cuts at: 293 3599 293
Size: 3306 776
SfcI C'TryA_G
Cuts at: 33 1125 1810 2803 2994 33
Size: 1092 685 993 191 1121
Fragments arranged by size:
1121 1092 993 685 191
SfoI GGC'GCC
Cuts at: 113 1514 1762 113
Size: 1401 248 2433
Fragments arranged by size:
2433 1401 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4082
SmaI CCC'GGG
Cuts at: 523 1104 523
Size: 581 3501
SmlI C'TyrA_G
Cuts at: 688 1237 2152 2644 2906 688
Size: 549 915 492 262 1864
Fragments arranged by size:
1864 915 549 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4082
SphI G_CATG'C
Cuts at: 1135 1635 1135
Size: 500 3582
StyI C'CwwG_G
Cuts at: 107 1216 1570 107
Size: 1109 354 2619
Fragments arranged by size:
2619 1109 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 879 1665 1798 2257 2466 879
Size: 786 133 459 209 2495
Fragments arranged by size:
2495 786 459 209 133
TatI w'GTAC_w
Cuts at: 291 3597 291
Size: 3306 776
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3599 3631 3599
Size: 32 4050
Tth111I GACn'n_nGTC
Cuts at: 1879 1879
Size: 4082
XbaI T'CTAG_A
Cuts at: 1113 1113
Size: 4082
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1577 1577
Size: 4082
XmaI C'CCGG_G
Cuts at: 521 1102 521
Size: 581 3501
XmnI GAAnn'nnTTC
Cuts at: 232 275 232
Size: 43 4039
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BpmI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI