Restriction map for
pF1KB9445
(Circular) (Six-base) MAPSORT of: pF1KB9445.seq Check: 7657 from: 1 to: 5129
pF1KB9445 5129 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 2167 2167
Size: 5129
Acc65I G'GTAC_C
Cuts at: 2145 2145
Size: 5129
AclI AA'CG_TT
Cuts at: 4969 4969
Size: 5129
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 902 2954 3386 4133 4401 902
Size: 2052 432 747 268 1630
Fragments arranged by size:
2052 1630 747 432 268
AflIII A'CryG_T
Cuts at: 2481 3585 4562 2481
Size: 1104 977 3048
Fragments arranged by size:
3048 1104 977
AgeI A'CCGG_T
Cuts at: 3517 3517
Size: 5129
AlwNI CAG_nnn'CTG
Cuts at: 775 1372 2434 4001 775
Size: 597 1062 1567 1903
Fragments arranged by size:
1903 1567 1062 597
ApaLI G'TGCA_C
Cuts at: 3899 3899
Size: 5129
ApoI r'AATT_y
Cuts at: 232 347 439 1277 1730 2101 2133 4482 4738
Size: 115 92 838 453 371 32 2349 256
Cuts at: 4738 232
Size: 623
Fragments arranged by size:
2349 838 623 453 371 256 115 92
32
AseI AT'TA_AT
Cuts at: 20 1219 20
Size: 1199 3930
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5129
AvaI C'yCGr_G
Cuts at: 2149 2149
Size: 5129
BamHI G'GATC_C
Cuts at: 2154 2154
Size: 5129
BanI G'GyrC_C
Cuts at: 2145 2559 2807 2842 2145
Size: 414 248 35 4432
Fragments arranged by size:
4432 414 248 35
BanII G_rGCy'C
Cuts at: 243 2143 3173 243
Size: 1900 1030 2199
Fragments arranged by size:
2199 1900 1030
BbeI G_GCGC'C
Cuts at: 2563 2811 2563
Size: 248 4881
BbsI GAAGACnn'nnnn_
Cuts at: 230 1796 230
Size: 1566 3563
BciVI GTATCCnnnnn_n'
Cuts at: 1682 3027 3788 5129 1682
Size: 1345 761 1341 1682
Fragments arranged by size:
1682 1345 1341 761
BclI T'GATC_A
Cuts at: 1551 2649 1551
Size: 1098 4031
BfrBI ATG'CAT
Cuts at: 1900 1900
Size: 5129
BglII A'GATC_T
Cuts at: 335 2644 4681 335
Size: 2309 2037 783
Fragments arranged by size:
2309 2037 783
BlpI GC'TnA_GC
Cuts at: 2241 2241
Size: 5129
Bme1580I G_kGCm'C
Cuts at: 2754 2847 3903 2754
Size: 93 1056 3980
Fragments arranged by size:
3980 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2341 2422 2757 4901 2341
Size: 81 335 2144 2569
Fragments arranged by size:
2569 2144 335 81
BmtI G_CTAG'C
Cuts at: 809 809
Size: 5129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3496 3496
Size: 5129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1122 1967 1122
Size: 845 4284
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1504 2305 3184 3676 3974 1504
Size: 801 879 492 298 2659
Fragments arranged by size:
2659 879 801 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4833 4833
Size: 5129
BsaAI yAC'GTr
Cuts at: 2482 2482
Size: 5129
BsaBI GATnn'nnATC
Cuts at: 6 2643 4680 6
Size: 2637 2037 455
Fragments arranged by size:
2637 2037 455
BsaHI Gr'CG_yC
Cuts at: 2560 2808 3510 5006 2560
Size: 248 702 1496 2683
Fragments arranged by size:
2683 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2318 2839 3517 3791 3938 4349 2318
Size: 521 678 274 147 411 3098
Fragments arranged by size:
3098 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1654 1684 1738 1768 1654
Size: 30 54 30 5015
Fragments arranged by size:
5015 54 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1108 1111 1114 1108
Size: 3 3 5123
Fragments arranged by size:
5123 3 3
BseYI C'CCAG_C
Cuts at: 298 2554 3312 3889 4385 298
Size: 2256 758 577 496 1042
Fragments arranged by size:
2256 1042 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 1057
Size: 5129
BsiEI CG_ry'CG
Cuts at: 104 1154 2671 2717 3925 104
Size: 1050 1517 46 1208 1308
Fragments arranged by size:
1517 1308 1208 1050 46
BsiHKAI G_wGCw'C
Cuts at: 2143 2921 3111 3903 2143
Size: 778 190 792 3369
Fragments arranged by size:
3369 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 243 2143 2754 2847 2921 3111 3173 3903 243
Size: 1900 611 93 74 190 62 730 1469
Fragments arranged by size:
1900 1469 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4349 4349
Size: 5129
BspHI T'CATG_A
Cuts at: 4305 4305
Size: 5129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1865 2179 2393 2695 3076 1865
Size: 314 214 302 381 3918
Fragments arranged by size:
3918 381 314 302 214
BsrBI CCG'CTC
Cuts at: 50 3421 3475 4959 5124 50
Size: 3371 54 1484 165 55
Fragments arranged by size:
3371 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1940 3041 1940
Size: 1101 4028
BsrFI r'CCGG_y
Cuts at: 3127 3308 3517 3127
Size: 181 209 4739
Fragments arranged by size:
4739 209 181
BssSI C'ACGA_G
Cuts at: 3400 3758 3400
Size: 358 4771
BstAPI GCAn_nnn'nTGC
Cuts at: 802 2506 802
Size: 1704 3425
BstBI TT'CG_AA
Cuts at: 3490 3490
Size: 5129
BstXI CCAn_nnnn'nTGG
Cuts at: 583 2024 583
Size: 1441 3688
BstYI r'GATC_y
Cuts at: 1 335 1833 2154 2621 2644 2979 3225 4237
Size: 334 1498 321 467 23 335 246 1012
Cuts at: 4237 4323 4335 4681 1
Size: 86 12 346 449
Fragments arranged by size:
1498 1012 467 449 346 335 334 321
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 189 107
Size: 82 5047
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3079 3260 3423 3079
Size: 181 163 4785
Fragments arranged by size:
4785 181 163
BtsI GCAGTG_nn'
Cuts at: 2372 2372
Size: 5129
DraI TTT'AAA
Cuts at: 2067 2128 2067
Size: 61 5068
DraIII CAC_nnn'GTG
Cuts at: 802 802
Size: 5129
DrdI GACnn_nn'nnGTC
Cuts at: 1153 2835 3693 1153
Size: 1682 858 2589
Fragments arranged by size:
2589 1682 858
EaeI y'GGCC_r
Cuts at: 186 1151 2714 2888 3279 3306 4651 186
Size: 965 1563 174 391 27 1345 664
Fragments arranged by size:
1563 1345 965 664 391 174 27
EagI C'GGCC_G
Cuts at: 1151 2714 1151
Size: 1563 3566
EarI CTCTTCn'nnn_
Cuts at: 202 292 1023 1085 3152 3362 202
Size: 90 731 62 2067 210 1969
Fragments arranged by size:
2067 1969 731 210 90 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3337 3647 3793 4939 3337
Size: 310 146 1146 3527
Fragments arranged by size:
3527 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2141 2141
Size: 5129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 902 1122 1967 2954 3386 4133 4401 902
Size: 220 845 987 432 747 268 1630
Fragments arranged by size:
1630 987 845 747 432 268 220
EcoNI CCTnn'n_nnAGG
Cuts at: 1466 1985 1466
Size: 519 4610
EcoO109I rG'GnC_Cy
Cuts at: 772 2268 772
Size: 1496 3633
EcoRI G'AATT_C
Cuts at: 2133 2133
Size: 5129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1276 3148 1276
Size: 1872 3257
FspI TGC'GCA
Cuts at: 2910 2910
Size: 5129
HaeII r_GCGC'y
Cuts at: 2563 2811 3833 4812 2563
Size: 248 1022 979 2880
Fragments arranged by size:
2880 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 320 352 2971 3001 3003 3033 320
Size: 32 2619 30 2 30 2416
Fragments arranged by size:
2619 2416 32 30 30 2
HincII GTy'rAC
Cuts at: 1965 2168 1965
Size: 203 4926
HindIII A'AGCT_T
Cuts at: 1629 1852 1629
Size: 223 4906
KasI G'GCGC_C
Cuts at: 2559 2807 2559
Size: 248 4881
KpnI G_GTAC'C
Cuts at: 2149 2149
Size: 5129
MluI A'CGCG_T
Cuts at: 4562 4562
Size: 5129
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 949 3800 3984 949
Size: 2851 184 2094
Fragments arranged by size:
2851 2094 184
MscI TGG'CCA
Cuts at: 188 2890 188
Size: 2702 2427
MslI CAynn'nnrTG
Cuts at: 3118 3245 3118
Size: 127 5002
MspA1I CmG'CkG
Cuts at: 2240 2554 2914 3927 4172 2240
Size: 314 360 1013 245 3197
Fragments arranged by size:
3197 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3310 3310
Size: 5129
NarI GG'CG_CC
Cuts at: 2560 2808 2560
Size: 248 4881
NcoI C'CATG_G
Cuts at: 107 189 107
Size: 82 5047
NdeI CA'TA_TG
Cuts at: 1010 1614 1010
Size: 604 4525
NgoMIV G'CCGG_C
Cuts at: 3308 3308
Size: 5129
NheI G'CTAG_C
Cuts at: 805 805
Size: 5129
NsiI A_TGCA'T
Cuts at: 1902 1902
Size: 5129
NspI r_CATG'y
Cuts at: 2182 2682 3589 2182
Size: 500 907 3722
Fragments arranged by size:
3722 907 500
PacI TTA_AT'TAA
Cuts at: 413 413
Size: 5129
PciI A'CATG_T
Cuts at: 3585 3585
Size: 5129
PmeI GTTT'AAAC
Cuts at: 2128 2128
Size: 5129
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4268 4300 4268
Size: 32 5097
PsiI TTA'TAA
Cuts at: 325 607 2107 325
Size: 282 1500 3347
Fragments arranged by size:
3347 1500 282
PstI C_TGCA'G
Cuts at: 2176 2861 2176
Size: 685 4444
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5129
PvuII CAG'CTG
Cuts at: 2554 2914 2554
Size: 360 4769
RsrII CG'GwC_CG
Cuts at: 3324 3324
Size: 5129
SacI G_AGCT'C
Cuts at: 2143 2143
Size: 5129
SalI G'TCGA_C
Cuts at: 2166 2166
Size: 5129
SapI GCTCTTCn'nnn_
Cuts at: 292 3152 3362 292
Size: 2860 210 2059
Fragments arranged by size:
2860 2059 210
SbfI CC_TGCA'GG
Cuts at: 2176 2176
Size: 5129
ScaI AGT'ACT
Cuts at: 148 2091 4646 148
Size: 1943 2555 631
Fragments arranged by size:
2555 1943 631
SfcI C'TryA_G
Cuts at: 33 134 363 2172 2857 3850 4041 33
Size: 101 229 1809 685 993 191 1121
Fragments arranged by size:
1809 1121 993 685 229 191 101
SfoI GGC'GCC
Cuts at: 2561 2809 2561
Size: 248 4881
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5129
SmaI CCC'GGG
Cuts at: 2151 2151
Size: 5129
SmlI C'TyrA_G
Cuts at: 1483 2284 3199 3691 3953 1483
Size: 801 915 492 262 2659
Fragments arranged by size:
2659 915 801 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5129
SphI G_CATG'C
Cuts at: 2182 2682 2182
Size: 500 4629
SspI AAT'ATT
Cuts at: 252 945 252
Size: 693 4436
StyI C'CwwG_G
Cuts at: 107 189 2263 2617 107
Size: 82 2074 354 2619
Fragments arranged by size:
2619 2074 354 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 242 2712 2845 3304 3513 242
Size: 2470 133 459 209 1858
Fragments arranged by size:
2470 1858 459 209 133
TatI w'GTAC_w
Cuts at: 146 2089 4644 146
Size: 1943 2555 631
Fragments arranged by size:
2555 1943 631
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4646 4678 4646
Size: 32 5097
Tth111I GACn'n_nGTC
Cuts at: 2926 2926
Size: 5129
XbaI T'CTAG_A
Cuts at: 2160 2160
Size: 5129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2624 2624
Size: 5129
XmaI C'CCGG_G
Cuts at: 2149 2149
Size: 5129
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NsiI NspI PacI
PciI PmeI PpiI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HpaI MfeI
NotI NruI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI