Restriction map for
pF1KB9436
(Circular) (Six-base) MAPSORT of: pF1KB9436.seq Check: 4682 from: 1 to: 3551
pF1KB9436 3551 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 276 276
Size: 3551
AccI GT'mk_AC
Cuts at: 589 589
Size: 3551
Acc65I G'GTAC_C
Cuts at: 567 567
Size: 3551
AclI AA'CG_TT
Cuts at: 3391 3391
Size: 3551
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1376 1808 2555 2823 1376
Size: 432 747 268 2104
Fragments arranged by size:
2104 747 432 268
AflIII A'CryG_T
Cuts at: 903 2007 2984 903
Size: 1104 977 1470
Fragments arranged by size:
1470 1104 977
AgeI A'CCGG_T
Cuts at: 1939 1939
Size: 3551
AlwNI CAG_nnn'CTG
Cuts at: 177 366 459 856 2423 177
Size: 189 93 397 1567 1305
Fragments arranged by size:
1567 1305 397 189 93
ApaLI G'TGCA_C
Cuts at: 2321 2321
Size: 3551
ApoI r'AATT_y
Cuts at: 555 2904 3160 555
Size: 2349 256 946
Fragments arranged by size:
2349 946 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3551
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3551
AvaI C'yCGr_G
Cuts at: 571 571
Size: 3551
BamHI G'GATC_C
Cuts at: 576 576
Size: 3551
BanI G'GyrC_C
Cuts at: 249 567 981 1229 1264 249
Size: 318 414 248 35 2536
Fragments arranged by size:
2536 414 318 248 35
BanII G_rGCy'C
Cuts at: 439 565 1595 439
Size: 126 1030 2395
Fragments arranged by size:
2395 1030 126
BbeI G_GCGC'C
Cuts at: 253 985 1233 253
Size: 732 248 2571
Fragments arranged by size:
2571 732 248
BbvCI CC'TCA_GC
Cuts at: 206 206
Size: 3551
BciVI GTATCCnnnnn_n'
Cuts at: 1449 2210 3551 1449
Size: 761 1341 1449
Fragments arranged by size:
1449 1341 761
BclI T'GATC_A
Cuts at: 1071 1071
Size: 3551
BfrBI ATG'CAT
Cuts at: 332 332
Size: 3551
BglII A'GATC_T
Cuts at: 1066 3103 1066
Size: 2037 1514
BlpI GC'TnA_GC
Cuts at: 663 663
Size: 3551
Bme1580I G_kGCm'C
Cuts at: 1176 1269 2325 1176
Size: 93 1056 2402
Fragments arranged by size:
2402 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 763 844 1179 3323 763
Size: 81 335 2144 991
Fragments arranged by size:
2144 991 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 312 1918 312
Size: 1606 1945
Bpu10I CC'TnA_GC
Cuts at: 206 433 206
Size: 227 3324
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 1606 2098 2396 727
Size: 879 492 298 1882
Fragments arranged by size:
1882 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3255 3255
Size: 3551
BsaAI yAC'GTr
Cuts at: 904 904
Size: 3551
BsaBI GATnn'nnATC
Cuts at: 6 1065 3102 6
Size: 1059 2037 455
Fragments arranged by size:
2037 1059 455
BsaHI Gr'CG_yC
Cuts at: 250 982 1230 1932 3428 250
Size: 732 248 702 1496 373
Fragments arranged by size:
1496 732 702 373 248
BsaWI w'CCGG_w
Cuts at: 740 1261 1939 2213 2360 2771 740
Size: 521 678 274 147 411 1520
Fragments arranged by size:
1520 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 194 194
Size: 3551
BseYI C'CCAG_C
Cuts at: 118 976 1734 2311 2807 118
Size: 858 758 577 496 862
Fragments arranged by size:
862 858 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1093 1139 2347 104
Size: 989 46 1208 1308
Fragments arranged by size:
1308 1208 989 46
BsiHKAI G_wGCw'C
Cuts at: 565 1343 1533 2325 565
Size: 778 190 792 1791
Fragments arranged by size:
1791 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 439 565 1176 1269 1343 1533 1595 2325 439
Size: 126 611 93 74 190 62 730 1665
Fragments arranged by size:
1665 730 611 190 126 93 74 62
BspEI T'CCGG_A
Cuts at: 2771 2771
Size: 3551
BspHI T'CATG_A
Cuts at: 2727 2727
Size: 3551
BspMI ACCTGCnnnn'nnnn_
Cuts at: 276 601 815 1117 1498 276
Size: 325 214 302 381 2329
Fragments arranged by size:
2329 381 325 302 214
BsrBI CCG'CTC
Cuts at: 50 1843 1897 3381 3546 50
Size: 1793 54 1484 165 55
Fragments arranged by size:
1793 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1463 1463
Size: 3551
BsrFI r'CCGG_y
Cuts at: 216 246 1549 1730 1939 216
Size: 30 1303 181 209 1828
Fragments arranged by size:
1828 1303 209 181 30
BssSI C'ACGA_G
Cuts at: 1822 2180 1822
Size: 358 3193
BstAPI GCAn_nnn'nTGC
Cuts at: 459 928 459
Size: 469 3082
BstBI TT'CG_AA
Cuts at: 530 1912 530
Size: 1382 2169
BstYI r'GATC_y
Cuts at: 1 576 1043 1066 1401 1647 2659 2745 2757
Size: 575 467 23 335 246 1012 86 12
Cuts at: 2757 3103 1
Size: 346 449
Fragments arranged by size:
1012 575 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 253 107
Size: 146 3405
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1501 1682 1845 1501
Size: 181 163 3207
Fragments arranged by size:
3207 181 163
BtsI GCAGTG_nn'
Cuts at: 794 794
Size: 3551
DraI TTT'AAA
Cuts at: 550 550
Size: 3551
DrdI GACnn_nn'nnGTC
Cuts at: 1257 2115 1257
Size: 858 2693
EaeI y'GGCC_r
Cuts at: 1136 1310 1701 1728 3073 1136
Size: 174 391 27 1345 1614
Fragments arranged by size:
1614 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1136 1136
Size: 3551
EarI CTCTTCn'nnn_
Cuts at: 281 1574 1784 281
Size: 1293 210 2048
Fragments arranged by size:
2048 1293 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 157 1759 2069 2215 3361 157
Size: 1602 310 146 1146 347
Fragments arranged by size:
1602 1146 347 310 146
EcoICRI GAG'CTC
Cuts at: 563 563
Size: 3551
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1376 1808 2555 2823 1376
Size: 432 747 268 2104
Fragments arranged by size:
2104 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 363 690 363
Size: 327 3224
EcoRI G'AATT_C
Cuts at: 555 555
Size: 3551
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1570 1570
Size: 3551
FspI TGC'GCA
Cuts at: 400 1332 400
Size: 932 2619
HaeII r_GCGC'y
Cuts at: 253 985 1233 2255 3234 253
Size: 732 248 1022 979 570
Fragments arranged by size:
1022 979 732 570 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1393 1423 1425 1455 1393
Size: 30 2 30 3489
Fragments arranged by size:
3489 30 30 2
HincII GTy'rAC
Cuts at: 590 590
Size: 3551
KasI G'GCGC_C
Cuts at: 249 981 1229 249
Size: 732 248 2571
Fragments arranged by size:
2571 732 248
KpnI G_GTAC'C
Cuts at: 571 571
Size: 3551
MluI A'CGCG_T
Cuts at: 2984 2984
Size: 3551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 191 2222 2406 191
Size: 2031 184 1336
Fragments arranged by size:
2031 1336 184
MscI TGG'CCA
Cuts at: 1312 1312
Size: 3551
MslI CAynn'nnrTG
Cuts at: 1540 1667 1540
Size: 127 3424
MspA1I CmG'CkG
Cuts at: 177 195 459 662 976 1336 2349 2594 177
Size: 18 264 203 314 360 1013 245 1134
Fragments arranged by size:
1134 1013 360 314 264 245 203 18
NaeI GCC'GGC
Cuts at: 248 1732 248
Size: 1484 2067
NarI GG'CG_CC
Cuts at: 250 982 1230 250
Size: 732 248 2571
Fragments arranged by size:
2571 732 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3551
NgoMIV G'CCGG_C
Cuts at: 246 1730 246
Size: 1484 2067
NsiI A_TGCA'T
Cuts at: 334 334
Size: 3551
NspI r_CATG'y
Cuts at: 528 604 1104 2011 528
Size: 76 500 907 2068
Fragments arranged by size:
2068 907 500 76
PciI A'CATG_T
Cuts at: 2007 2007
Size: 3551
PfoI T'CCnGG_A
Cuts at: 389 470 389
Size: 81 3470
PmeI GTTT'AAAC
Cuts at: 550 550
Size: 3551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2690 2722 2690
Size: 32 3519
PpuMI rG'GwC_Cy
Cuts at: 363 363
Size: 3551
PstI C_TGCA'G
Cuts at: 464 598 1283 464
Size: 134 685 2732
Fragments arranged by size:
2732 685 134
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3551
PvuII CAG'CTG
Cuts at: 459 976 1336 459
Size: 517 360 2674
Fragments arranged by size:
2674 517 360
RsrII CG'GwC_CG
Cuts at: 1746 1746
Size: 3551
SacI G_AGCT'C
Cuts at: 565 565
Size: 3551
SalI G'TCGA_C
Cuts at: 588 588
Size: 3551
SapI GCTCTTCn'nnn_
Cuts at: 1574 1784 1574
Size: 210 3341
SbfI CC_TGCA'GG
Cuts at: 598 598
Size: 3551
ScaI AGT'ACT
Cuts at: 3068 3068
Size: 3551
SfcI C'TryA_G
Cuts at: 33 460 594 1279 2272 2463 33
Size: 427 134 685 993 191 1121
Fragments arranged by size:
1121 993 685 427 191 134
SfoI GGC'GCC
Cuts at: 251 983 1231 251
Size: 732 248 2571
Fragments arranged by size:
2571 732 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3551
SgrAI Cr'CCGG_yG
Cuts at: 246 246
Size: 3551
SmaI CCC'GGG
Cuts at: 573 573
Size: 3551
SmlI C'TyrA_G
Cuts at: 706 1621 2113 2375 706
Size: 915 492 262 1882
Fragments arranged by size:
1882 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3551
SphI G_CATG'C
Cuts at: 604 1104 604
Size: 500 3051
StyI C'CwwG_G
Cuts at: 107 539 685 1039 107
Size: 432 146 354 2619
Fragments arranged by size:
2619 432 354 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1134 1267 1726 1935 1134
Size: 133 459 209 2750
Fragments arranged by size:
2750 459 209 133
TatI w'GTAC_w
Cuts at: 509 3066 509
Size: 2557 994
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3068 3100 3068
Size: 32 3519
Tth111I GACn'n_nGTC
Cuts at: 1348 1348
Size: 3551
XbaI T'CTAG_A
Cuts at: 582 582
Size: 3551
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1046 1046
Size: 3551
XmaI C'CCGG_G
Cuts at: 571 571
Size: 3551
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BglI BmgBI BmtI
BpmI BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI