Restriction map for
pF1KB9435
(Circular) (Six-base) MAPSORT of: pF1KB9435.seq Check: 8262 from: 1 to: 3761
pF1KB9435 3761 bp
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AccI GT'mk_AC
Cuts at: 799 799
Size: 3761
Acc65I G'GTAC_C
Cuts at: 777 777
Size: 3761
AclI AA'CG_TT
Cuts at: 3601 3601
Size: 3761
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 368 1586 2018 2765 3033 188
Size: 180 1218 432 747 268 916
Fragments arranged by size:
1218 916 747 432 268 180
AflIII A'CryG_T
Cuts at: 314 1113 2217 3194 314
Size: 799 1104 977 881
Fragments arranged by size:
1104 977 881 799
AgeI A'CCGG_T
Cuts at: 2149 2149
Size: 3761
AlwNI CAG_nnn'CTG
Cuts at: 1066 2633 1066
Size: 1567 2194
ApaLI G'TGCA_C
Cuts at: 2531 2531
Size: 3761
ApoI r'AATT_y
Cuts at: 337 765 3114 3370 337
Size: 428 2349 256 728
Fragments arranged by size:
2349 728 428 256
AseI AT'TA_AT
Cuts at: 20 727 20
Size: 707 3054
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3761
AvaI C'yCGr_G
Cuts at: 781 781
Size: 3761
BamHI G'GATC_C
Cuts at: 786 786
Size: 3761
BanI G'GyrC_C
Cuts at: 711 777 1191 1439 1474 711
Size: 66 414 248 35 2998
Fragments arranged by size:
2998 414 248 66 35
BanII G_rGCy'C
Cuts at: 775 1805 775
Size: 1030 2731
BbeI G_GCGC'C
Cuts at: 1195 1443 1195
Size: 248 3513
BbvCI CC'TCA_GC
Cuts at: 121 121
Size: 3761
BciVI GTATCCnnnnn_n'
Cuts at: 1659 2420 3761 1659
Size: 761 1341 1659
Fragments arranged by size:
1659 1341 761
BclI T'GATC_A
Cuts at: 1281 1281
Size: 3761
BglII A'GATC_T
Cuts at: 1276 3313 1276
Size: 2037 1724
BlpI GC'TnA_GC
Cuts at: 873 873
Size: 3761
Bme1580I G_kGCm'C
Cuts at: 1386 1479 2535 1386
Size: 93 1056 2612
Fragments arranged by size:
2612 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 405 973 1054 1389 3533 405
Size: 568 81 335 2144 633
Fragments arranged by size:
2144 633 568 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 280 2128 280
Size: 1848 1913
Bpu10I CC'TnA_GC
Cuts at: 121 121
Size: 3761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 937 1816 2308 2606 937
Size: 879 492 298 2092
Fragments arranged by size:
2092 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3465 3465
Size: 3761
BsaAI yAC'GTr
Cuts at: 1114 1114
Size: 3761
BsaBI GATnn'nnATC
Cuts at: 6 1275 3312 6
Size: 1269 2037 455
Fragments arranged by size:
2037 1269 455
BsaHI Gr'CG_yC
Cuts at: 1192 1440 2142 3638 1192
Size: 248 702 1496 1315
Fragments arranged by size:
1496 1315 702 248
BsaWI w'CCGG_w
Cuts at: 950 1471 2149 2423 2570 2981 950
Size: 521 678 274 147 411 1730
Fragments arranged by size:
1730 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 646 646
Size: 3761
BseYI C'CCAG_C
Cuts at: 1186 1944 2521 3017 1186
Size: 758 577 496 1930
Fragments arranged by size:
1930 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 327 422 327
Size: 95 3666
BsiEI CG_ry'CG
Cuts at: 104 1303 1349 2557 104
Size: 1199 46 1208 1308
Fragments arranged by size:
1308 1208 1199 46
BsiHKAI G_wGCw'C
Cuts at: 775 1553 1743 2535 775
Size: 778 190 792 2001
Fragments arranged by size:
2001 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 775 1386 1479 1553 1743 1805 2535 775
Size: 611 93 74 190 62 730 2001
Fragments arranged by size:
2001 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2981 2981
Size: 3761
BspHI T'CATG_A
Cuts at: 2937 2937
Size: 3761
BspMI ACCTGCnnnn'nnnn_
Cuts at: 364 811 1025 1327 1708 364
Size: 447 214 302 381 2417
Fragments arranged by size:
2417 447 381 302 214
BsrBI CCG'CTC
Cuts at: 50 131 2053 2107 3591 3756 50
Size: 81 1922 54 1484 165 55
Fragments arranged by size:
1922 1484 165 81 55 54
BsrDI GCAATG_nn'
Cuts at: 1673 1673
Size: 3761
BsrFI r'CCGG_y
Cuts at: 1759 1940 2149 1759
Size: 181 209 3371
Fragments arranged by size:
3371 209 181
BssSI C'ACGA_G
Cuts at: 2032 2390 2032
Size: 358 3403
BstAPI GCAn_nnn'nTGC
Cuts at: 1138 1138
Size: 3761
BstBI TT'CG_AA
Cuts at: 2122 2122
Size: 3761
BstYI r'GATC_y
Cuts at: 1 172 261 786 1253 1276 1611 1857 2869
Size: 171 89 525 467 23 335 246 1012
Cuts at: 2869 2955 2967 3313 1
Size: 86 12 346 449
Fragments arranged by size:
1012 525 467 449 346 335 246 171
89 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 251 251
Size: 3761
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1711 1892 2055 1711
Size: 181 163 3417
Fragments arranged by size:
3417 181 163
BtsI GCAGTG_nn'
Cuts at: 1004 1004
Size: 3761
DraI TTT'AAA
Cuts at: 760 760
Size: 3761
DrdI GACnn_nn'nnGTC
Cuts at: 1467 2325 1467
Size: 858 2903
EaeI y'GGCC_r
Cuts at: 1346 1520 1911 1938 3283 1346
Size: 174 391 27 1345 1824
Fragments arranged by size:
1824 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1346 1346
Size: 3761
EarI CTCTTCn'nnn_
Cuts at: 138 722 1784 1994 138
Size: 584 1062 210 1905
Fragments arranged by size:
1905 1062 584 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1969 2279 2425 3571 1969
Size: 310 146 1146 2159
Fragments arranged by size:
2159 1146 310 146
EcoICRI GAG'CTC
Cuts at: 773 773
Size: 3761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 188 368 1586 2018 2765 3033 188
Size: 180 1218 432 747 268 916
Fragments arranged by size:
1218 916 747 432 268 180
EcoO109I rG'GnC_Cy
Cuts at: 900 900
Size: 3761
EcoRI G'AATT_C
Cuts at: 337 765 337
Size: 428 3333
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 499 1780 499
Size: 1281 2480
FspI TGC'GCA
Cuts at: 1542 1542
Size: 3761
HaeII r_GCGC'y
Cuts at: 1195 1443 2465 3444 1195
Size: 248 1022 979 1512
Fragments arranged by size:
1512 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1603 1633 1635 1665 1603
Size: 30 2 30 3699
Fragments arranged by size:
3699 30 30 2
HincII GTy'rAC
Cuts at: 800 800
Size: 3761
KasI G'GCGC_C
Cuts at: 1191 1439 1191
Size: 248 3513
KpnI G_GTAC'C
Cuts at: 781 781
Size: 3761
MluI A'CGCG_T
Cuts at: 3194 3194
Size: 3761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2432 2616 2432
Size: 184 3577
MscI TGG'CCA
Cuts at: 1522 1522
Size: 3761
MslI CAynn'nnrTG
Cuts at: 182 1750 1877 182
Size: 1568 127 2066
Fragments arranged by size:
2066 1568 127
MspA1I CmG'CkG
Cuts at: 624 872 1186 1546 2559 2804 624
Size: 248 314 360 1013 245 1581
Fragments arranged by size:
1581 1013 360 314 248 245
NaeI GCC'GGC
Cuts at: 1942 1942
Size: 3761
NarI GG'CG_CC
Cuts at: 1192 1440 1192
Size: 248 3513
NgoMIV G'CCGG_C
Cuts at: 1940 1940
Size: 3761
NspI r_CATG'y
Cuts at: 202 318 814 1314 2221 202
Size: 116 496 500 907 1742
Fragments arranged by size:
1742 907 500 496 116
PciI A'CATG_T
Cuts at: 314 2217 314
Size: 1903 1858
PmeI GTTT'AAAC
Cuts at: 760 760
Size: 3761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2900 2932 2900
Size: 32 3729
PstI C_TGCA'G
Cuts at: 375 808 1493 375
Size: 433 685 2643
Fragments arranged by size:
2643 685 433
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3761
PvuII CAG'CTG
Cuts at: 624 1186 1546 624
Size: 562 360 2839
Fragments arranged by size:
2839 562 360
RsrII CG'GwC_CG
Cuts at: 1956 1956
Size: 3761
SacI G_AGCT'C
Cuts at: 775 775
Size: 3761
SalI G'TCGA_C
Cuts at: 798 798
Size: 3761
SapI GCTCTTCn'nnn_
Cuts at: 138 1784 1994 138
Size: 1646 210 1905
Fragments arranged by size:
1905 1646 210
SbfI CC_TGCA'GG
Cuts at: 808 808
Size: 3761
ScaI AGT'ACT
Cuts at: 3278 3278
Size: 3761
SfcI C'TryA_G
Cuts at: 33 371 804 1489 2482 2673 33
Size: 338 433 685 993 191 1121
Fragments arranged by size:
1121 993 685 433 338 191
SfoI GGC'GCC
Cuts at: 1193 1441 1193
Size: 248 3513
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3761
SmaI CCC'GGG
Cuts at: 783 783
Size: 3761
SmlI C'TyrA_G
Cuts at: 916 1831 2323 2585 916
Size: 915 492 262 2092
Fragments arranged by size:
2092 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3761
SphI G_CATG'C
Cuts at: 814 1314 814
Size: 500 3261
SspI AAT'ATT
Cuts at: 414 414
Size: 3761
StyI C'CwwG_G
Cuts at: 895 1249 895
Size: 354 3407
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1344 1477 1936 2145 1344
Size: 133 459 209 2960
Fragments arranged by size:
2960 459 209 133
TatI w'GTAC_w
Cuts at: 3276 3276
Size: 3761
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 3729
Tth111I GACn'n_nGTC
Cuts at: 1558 1558
Size: 3761
XbaI T'CTAG_A
Cuts at: 792 792
Size: 3761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1256 1256
Size: 3761
XmaI C'CCGG_G
Cuts at: 781 781
Size: 3761
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmgBI BmtI BpmI BsaXI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI