Restriction map for
pF1KB9434
(Circular) (Six-base) MAPSORT of: pF1KB9434.seq Check: 8117 from: 1 to: 3761
pF1KB9434 3761 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 667 667
Size: 3761
AccI GT'mk_AC
Cuts at: 799 799
Size: 3761
Acc65I G'GTAC_C
Cuts at: 777 777
Size: 3761
AclI AA'CG_TT
Cuts at: 3601 3601
Size: 3761
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 259 535 1586 2018 2765 3033 142
Size: 117 276 1051 432 747 268 870
Fragments arranged by size:
1051 870 747 432 276 268 117
AfeI AGC'GCT
Cuts at: 440 440
Size: 3761
AflIII A'CryG_T
Cuts at: 294 517 1113 2217 3194 294
Size: 223 596 1104 977 861
Fragments arranged by size:
1104 977 861 596 223
AgeI A'CCGG_T
Cuts at: 2149 2149
Size: 3761
AlwNI CAG_nnn'CTG
Cuts at: 1066 2633 1066
Size: 1567 2194
ApaI G_GGCC'C
Cuts at: 388 388
Size: 3761
ApaLI G'TGCA_C
Cuts at: 2531 2531
Size: 3761
ApoI r'AATT_y
Cuts at: 166 765 3114 3370 166
Size: 599 2349 256 557
Fragments arranged by size:
2349 599 557 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3761
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3761
AvaI C'yCGr_G
Cuts at: 225 781 225
Size: 556 3205
BamHI G'GATC_C
Cuts at: 786 786
Size: 3761
BanI G'GyrC_C
Cuts at: 334 777 1191 1439 1474 334
Size: 443 414 248 35 2621
Fragments arranged by size:
2621 443 414 248 35
BanII G_rGCy'C
Cuts at: 210 388 775 1805 210
Size: 178 387 1030 2166
Fragments arranged by size:
2166 1030 387 178
BbeI G_GCGC'C
Cuts at: 1195 1443 1195
Size: 248 3513
BciVI GTATCCnnnnn_n'
Cuts at: 1659 2420 3761 1659
Size: 761 1341 1659
Fragments arranged by size:
1659 1341 761
BclI T'GATC_A
Cuts at: 1281 1281
Size: 3761
BglII A'GATC_T
Cuts at: 554 1276 3313 554
Size: 722 2037 1002
Fragments arranged by size:
2037 1002 722
BlpI GC'TnA_GC
Cuts at: 873 873
Size: 3761
Bme1580I G_kGCm'C
Cuts at: 388 1386 1479 2535 388
Size: 998 93 1056 1614
Fragments arranged by size:
1614 1056 998 93
BmgBI CAC'GTC
Cuts at: 600 600
Size: 3761
BmrI ACTGGGnnnn_n'
Cuts at: 254 291 447 973 1054 1389 3533 254
Size: 37 156 526 81 335 2144 482
Fragments arranged by size:
2144 526 482 335 156 81 37
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 3761
Bpu10I CC'TnA_GC
Cuts at: 209 209
Size: 3761
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 937 1816 2308 2606 403
Size: 534 879 492 298 1558
Fragments arranged by size:
1558 879 534 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3465 3465
Size: 3761
BsaAI yAC'GTr
Cuts at: 1114 1114
Size: 3761
BsaBI GATnn'nnATC
Cuts at: 6 1275 3312 6
Size: 1269 2037 455
Fragments arranged by size:
2037 1269 455
BsaHI Gr'CG_yC
Cuts at: 1192 1440 2142 3638 1192
Size: 248 702 1496 1315
Fragments arranged by size:
1496 1315 702 248
BsaWI w'CCGG_w
Cuts at: 950 1471 2149 2423 2570 2981 950
Size: 521 678 274 147 411 1730
Fragments arranged by size:
1730 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1186 1944 2521 3017 1186
Size: 758 577 496 1930
Fragments arranged by size:
1930 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 271 336 659 181
Size: 90 65 323 3283
Fragments arranged by size:
3283 323 90 65
BsiEI CG_ry'CG
Cuts at: 104 621 1303 1349 2557 104
Size: 517 682 46 1208 1308
Fragments arranged by size:
1308 1208 682 517 46
BsiHKAI G_wGCw'C
Cuts at: 775 1553 1743 2535 775
Size: 778 190 792 2001
Fragments arranged by size:
2001 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 210 388 775 1386 1479 1553 1743 1805 2535
Size: 178 387 611 93 74 190 62 730
Cuts at: 2535 210
Size: 1436
Fragments arranged by size:
1436 730 611 387 190 178 93 74
62
BspEI T'CCGG_A
Cuts at: 2981 2981
Size: 3761
BspHI T'CATG_A
Cuts at: 2937 2937
Size: 3761
BspMI ACCTGCnnnn'nnnn_
Cuts at: 117 667 811 1025 1327 1708 117
Size: 550 144 214 302 381 2170
Fragments arranged by size:
2170 550 381 302 214 144
BsrBI CCG'CTC
Cuts at: 50 360 2053 2107 3591 3756 50
Size: 310 1693 54 1484 165 55
Fragments arranged by size:
1693 1484 310 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1673 1673
Size: 3761
BsrFI r'CCGG_y
Cuts at: 1759 1940 2149 1759
Size: 181 209 3371
Fragments arranged by size:
3371 209 181
BssSI C'ACGA_G
Cuts at: 2032 2390 2032
Size: 358 3403
BstAPI GCAn_nnn'nTGC
Cuts at: 1138 1138
Size: 3761
BstBI TT'CG_AA
Cuts at: 2122 2122
Size: 3761
BstYI r'GATC_y
Cuts at: 1 554 566 786 1253 1276 1611 1857 2869
Size: 553 12 220 467 23 335 246 1012
Cuts at: 2869 2955 2967 3313 1
Size: 86 12 346 449
Fragments arranged by size:
1012 553 467 449 346 335 246 220
86 23 12 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1711 1892 2055 1711
Size: 181 163 3417
Fragments arranged by size:
3417 181 163
BtsI GCAGTG_nn'
Cuts at: 1004 1004
Size: 3761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 503 538 503
Size: 35 3726
DraI TTT'AAA
Cuts at: 760 760
Size: 3761
DrdI GACnn_nn'nnGTC
Cuts at: 1467 2325 1467
Size: 858 2903
EaeI y'GGCC_r
Cuts at: 1346 1520 1911 1938 3283 1346
Size: 174 391 27 1345 1824
Fragments arranged by size:
1824 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1346 1346
Size: 3761
EarI CTCTTCn'nnn_
Cuts at: 1784 1994 1784
Size: 210 3551
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1969 2279 2425 3571 1969
Size: 310 146 1146 2159
Fragments arranged by size:
2159 1146 310 146
EcoICRI GAG'CTC
Cuts at: 773 773
Size: 3761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 259 535 1586 2018 2765 3033 142
Size: 117 276 1051 432 747 268 870
Fragments arranged by size:
1051 870 747 432 276 268 117
EcoNI CCTnn'n_nnAGG
Cuts at: 124 124
Size: 3761
EcoO109I rG'GnC_Cy
Cuts at: 384 385 900 384
Size: 1 515 3245
Fragments arranged by size:
3245 515 1
EcoRI G'AATT_C
Cuts at: 765 765
Size: 3761
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1780 1780
Size: 3761
FspI TGC'GCA
Cuts at: 1542 1542
Size: 3761
HaeII r_GCGC'y
Cuts at: 442 1195 1443 2465 3444 442
Size: 753 248 1022 979 759
Fragments arranged by size:
1022 979 759 753 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1603 1633 1635 1665 1603
Size: 30 2 30 3699
Fragments arranged by size:
3699 30 30 2
HincII GTy'rAC
Cuts at: 654 800 654
Size: 146 3615
KasI G'GCGC_C
Cuts at: 1191 1439 1191
Size: 248 3513
KpnI G_GTAC'C
Cuts at: 781 781
Size: 3761
MluI A'CGCG_T
Cuts at: 3194 3194
Size: 3761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 177 538 2432 2616 177
Size: 361 1894 184 1322
Fragments arranged by size:
1894 1322 361 184
MscI TGG'CCA
Cuts at: 1522 1522
Size: 3761
MslI CAynn'nnrTG
Cuts at: 631 691 1750 1877 631
Size: 60 1059 127 2515
Fragments arranged by size:
2515 1059 127 60
MspA1I CmG'CkG
Cuts at: 872 1186 1546 2559 2804 872
Size: 314 360 1013 245 1829
Fragments arranged by size:
1829 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1942 1942
Size: 3761
NarI GG'CG_CC
Cuts at: 1192 1440 1192
Size: 248 3513
NgoMIV G'CCGG_C
Cuts at: 1940 1940
Size: 3761
NspI r_CATG'y
Cuts at: 298 521 814 1314 2221 298
Size: 223 293 500 907 1838
Fragments arranged by size:
1838 907 500 293 223
PciI A'CATG_T
Cuts at: 294 517 2217 294
Size: 223 1700 1838
Fragments arranged by size:
1838 1700 223
PmeI GTTT'AAAC
Cuts at: 760 760
Size: 3761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2900 2932 2900
Size: 32 3729
PspOMI G'GGCC_C
Cuts at: 384 384
Size: 3761
PstI C_TGCA'G
Cuts at: 808 1493 808
Size: 685 3076
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3761
PvuII CAG'CTG
Cuts at: 1186 1546 1186
Size: 360 3401
RsrII CG'GwC_CG
Cuts at: 1956 1956
Size: 3761
SacI G_AGCT'C
Cuts at: 775 775
Size: 3761
SalI G'TCGA_C
Cuts at: 798 798
Size: 3761
SapI GCTCTTCn'nnn_
Cuts at: 1784 1994 1784
Size: 210 3551
SbfI CC_TGCA'GG
Cuts at: 808 808
Size: 3761
ScaI AGT'ACT
Cuts at: 3278 3278
Size: 3761
SfcI C'TryA_G
Cuts at: 33 804 1489 2482 2673 33
Size: 771 685 993 191 1121
Fragments arranged by size:
1121 993 771 685 191
SfoI GGC'GCC
Cuts at: 1193 1441 1193
Size: 248 3513
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3761
SmaI CCC'GGG
Cuts at: 783 783
Size: 3761
SmlI C'TyrA_G
Cuts at: 418 916 1831 2323 2585 418
Size: 498 915 492 262 1594
Fragments arranged by size:
1594 915 498 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3761
SphI G_CATG'C
Cuts at: 814 1314 814
Size: 500 3261
SspI AAT'ATT
Cuts at: 509 509
Size: 3761
StyI C'CwwG_G
Cuts at: 895 1249 895
Size: 354 3407
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 273 514 625 1344 1477 1936 2145 273
Size: 241 111 719 133 459 209 1889
Fragments arranged by size:
1889 719 459 241 209 133 111
TatI w'GTAC_w
Cuts at: 291 455 3276 291
Size: 164 2821 776
Fragments arranged by size:
2821 776 164
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 3729
Tth111I GACn'n_nGTC
Cuts at: 622 1558 622
Size: 936 2825
XbaI T'CTAG_A
Cuts at: 792 792
Size: 3761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1256 1256
Size: 3761
XmaI C'CCGG_G
Cuts at: 781 781
Size: 3761
XmnI GAAnn'nnTTC
Cuts at: 509 509
Size: 3761
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmtI BpmI
BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI