Restriction map for
pF1KB9433
(Circular) (Six-base) MAPSORT of: pF1KB9433.seq Check: 7842 from: 1 to: 3929
pF1KB9433 3929 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 967 967
Size: 3929
Acc65I G'GTAC_C
Cuts at: 945 945
Size: 3929
AclI AA'CG_TT
Cuts at: 3769 3769
Size: 3929
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 732 1754 2186 2933 3201 550
Size: 182 1022 432 747 268 1278
Fragments arranged by size:
1278 1022 747 432 268 182
AfeI AGC'GCT
Cuts at: 334 334
Size: 3929
AflIII A'CryG_T
Cuts at: 627 1281 2385 3362 627
Size: 654 1104 977 1194
Fragments arranged by size:
1194 1104 977 654
AgeI A'CCGG_T
Cuts at: 2317 2317
Size: 3929
AhdI GACnn_n'nnGTC
Cuts at: 165 485 165
Size: 320 3609
AlwNI CAG_nnn'CTG
Cuts at: 597 639 810 1234 2801 597
Size: 42 171 424 1567 1725
Fragments arranged by size:
1725 1567 424 171 42
ApaLI G'TGCA_C
Cuts at: 2699 2699
Size: 3929
ApoI r'AATT_y
Cuts at: 933 3282 3538 933
Size: 2349 256 1324
Fragments arranged by size:
2349 1324 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3929
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
AvaI C'yCGr_G
Cuts at: 135 226 949 135
Size: 91 723 3115
Fragments arranged by size:
3115 723 91
BamHI G'GATC_C
Cuts at: 954 954
Size: 3929
BanI G'GyrC_C
Cuts at: 215 945 1359 1607 1642 215
Size: 730 414 248 35 2502
Fragments arranged by size:
2502 730 414 248 35
BanII G_rGCy'C
Cuts at: 943 1973 943
Size: 1030 2899
BbeI G_GCGC'C
Cuts at: 1363 1611 1363
Size: 248 3681
BbsI GAAGACnn'nnnn_
Cuts at: 902 902
Size: 3929
BciVI GTATCCnnnnn_n'
Cuts at: 1827 2588 3929 1827
Size: 761 1341 1827
Fragments arranged by size:
1827 1341 761
BclI T'GATC_A
Cuts at: 1449 1449
Size: 3929
BglI GCCn_nnn'nGGC
Cuts at: 901 901
Size: 3929
BglII A'GATC_T
Cuts at: 1444 3481 1444
Size: 2037 1892
BlpI GC'TnA_GC
Cuts at: 232 703 712 1041 232
Size: 471 9 329 3120
Fragments arranged by size:
3120 471 329 9
Bme1580I G_kGCm'C
Cuts at: 1554 1647 2703 1554
Size: 93 1056 2780
Fragments arranged by size:
2780 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1141 1222 1557 3701 1141
Size: 81 335 2144 1369
Fragments arranged by size:
2144 1369 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 843 862 900 2296 843
Size: 19 38 1396 2476
Fragments arranged by size:
2476 1396 38 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 577
Size: 3929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1984 2476 2774 1105
Size: 879 492 298 2260
Fragments arranged by size:
2260 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3633 3633
Size: 3929
BsaAI yAC'GTr
Cuts at: 546 1282 546
Size: 736 3193
BsaBI GATnn'nnATC
Cuts at: 6 1443 3480 6
Size: 1437 2037 455
Fragments arranged by size:
2037 1437 455
BsaHI Gr'CG_yC
Cuts at: 904 1360 1608 2310 3806 904
Size: 456 248 702 1496 1027
Fragments arranged by size:
1496 1027 702 456 248
BsaWI w'CCGG_w
Cuts at: 207 1118 1639 2317 2591 2738 3149 207
Size: 911 521 678 274 147 411 987
Fragments arranged by size:
987 911 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 130 243 449 661 851 127
Size: 3 113 206 212 190 3205
Fragments arranged by size:
3205 212 206 190 113 3
BseYI C'CCAG_C
Cuts at: 618 1354 2112 2689 3185 618
Size: 736 758 577 496 1362
Fragments arranged by size:
1362 758 736 577 496
BsiEI CG_ry'CG
Cuts at: 104 509 1471 1517 2725 104
Size: 405 962 46 1208 1308
Fragments arranged by size:
1308 1208 962 405 46
BsiHKAI G_wGCw'C
Cuts at: 943 1721 1911 2703 943
Size: 778 190 792 2169
Fragments arranged by size:
2169 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 139 139
Size: 3929
Bsp1286I G_dGCh'C
Cuts at: 943 1554 1647 1721 1911 1973 2703 943
Size: 611 93 74 190 62 730 2169
Fragments arranged by size:
2169 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3149 3149
Size: 3929
BspHI T'CATG_A
Cuts at: 3105 3105
Size: 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 353 818 979 1193 1495 1876 353
Size: 465 161 214 302 381 2406
Fragments arranged by size:
2406 465 381 302 214 161
BsrBI CCG'CTC
Cuts at: 50 716 2221 2275 3759 3924 50
Size: 666 1505 54 1484 165 55
Fragments arranged by size:
1505 1484 666 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1841 1841
Size: 3929
BsrFI r'CCGG_y
Cuts at: 707 763 786 1927 2108 2317 707
Size: 56 23 1141 181 209 2319
Fragments arranged by size:
2319 1141 209 181 56 23
BssSI C'ACGA_G
Cuts at: 2200 2558 2200
Size: 358 3571
BstAPI GCAn_nnn'nTGC
Cuts at: 810 1306 810
Size: 496 3433
BstBI TT'CG_AA
Cuts at: 2290 2290
Size: 3929
BstEII G'GTnAC_C
Cuts at: 766 766
Size: 3929
BstYI r'GATC_y
Cuts at: 1 186 954 1421 1444 1779 2025 3037 3123
Size: 185 768 467 23 335 246 1012 86
Cuts at: 3123 3135 3481 1
Size: 12 346 449
Fragments arranged by size:
1012 768 467 449 346 335 246 185
86 23 12
BtgI C'CryG_G
Cuts at: 107 314 107
Size: 207 3722
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1879 2060 2223 1879
Size: 181 163 3585
Fragments arranged by size:
3585 181 163
BtsI GCAGTG_nn'
Cuts at: 396 637 1172 396
Size: 241 535 3153
Fragments arranged by size:
3153 535 241
DraI TTT'AAA
Cuts at: 928 928
Size: 3929
DrdI GACnn_nn'nnGTC
Cuts at: 1635 2493 1635
Size: 858 3071
EaeI y'GGCC_r
Cuts at: 506 1514 1688 2079 2106 3451 506
Size: 1008 174 391 27 1345 984
Fragments arranged by size:
1345 1008 984 391 174 27
EagI C'GGCC_G
Cuts at: 506 1514 506
Size: 1008 2921
EarI CTCTTCn'nnn_
Cuts at: 137 751 812 1952 2162 137
Size: 614 61 1140 210 1904
Fragments arranged by size:
1904 1140 614 210 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 199 278 432 2137 2447 2593 3739 199
Size: 79 154 1705 310 146 1146 389
Fragments arranged by size:
1705 1146 389 310 154 146 79
EcoICRI GAG'CTC
Cuts at: 941 941
Size: 3929
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 577 732 1754 2186 2933 3201 550
Size: 27 155 1022 432 747 268 1278
Fragments arranged by size:
1278 1022 747 432 268 155 27
EcoO109I rG'GnC_Cy
Cuts at: 244 807 1068 244
Size: 563 261 3105
Fragments arranged by size:
3105 563 261
EcoRI G'AATT_C
Cuts at: 933 933
Size: 3929
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1948 1948
Size: 3929
FspI TGC'GCA
Cuts at: 1710 1710
Size: 3929
HaeII r_GCGC'y
Cuts at: 336 407 1363 1611 2633 3612 336
Size: 71 956 248 1022 979 653
Fragments arranged by size:
1022 979 956 653 248 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 151 183 471 503 853 885 1771 1801 1803
Size: 32 288 32 350 32 886 30 2
Cuts at: 1803 1833 151
Size: 30 2247
Fragments arranged by size:
2247 886 350 288 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 968 968
Size: 3929
KasI G'GCGC_C
Cuts at: 1359 1607 1359
Size: 248 3681
KpnI G_GTAC'C
Cuts at: 949 949
Size: 3929
MluI A'CGCG_T
Cuts at: 627 3362 627
Size: 2735 1194
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2600 2784 2600
Size: 184 3745
MscI TGG'CCA
Cuts at: 1690 1690
Size: 3929
MslI CAynn'nnrTG
Cuts at: 1918 2045 1918
Size: 127 3802
MspA1I CmG'CkG
Cuts at: 316 455 511 1040 1354 1714 2727 2972 316
Size: 139 56 529 314 360 1013 245 1273
Fragments arranged by size:
1273 1013 529 360 314 245 139 56
NaeI GCC'GGC
Cuts at: 709 2110 709
Size: 1401 2528
NarI GG'CG_CC
Cuts at: 1360 1608 1360
Size: 248 3681
NcoI C'CATG_G
Cuts at: 107 107
Size: 3929
NgoMIV G'CCGG_C
Cuts at: 707 2108 707
Size: 1401 2528
NotI GC'GGCC_GC
Cuts at: 506 506
Size: 3929
NspI r_CATG'y
Cuts at: 982 1482 2389 982
Size: 500 907 2522
Fragments arranged by size:
2522 907 500
PciI A'CATG_T
Cuts at: 2385 2385
Size: 3929
PfoI T'CCnGG_A
Cuts at: 191 191
Size: 3929
PmeI GTTT'AAAC
Cuts at: 928 928
Size: 3929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3068 3100 3068
Size: 32 3897
PpuMI rG'GwC_Cy
Cuts at: 807 807
Size: 3929
PstI C_TGCA'G
Cuts at: 382 644 976 1661 382
Size: 262 332 685 2650
Fragments arranged by size:
2650 685 332 262
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3929
PvuII CAG'CTG
Cuts at: 1354 1714 1354
Size: 360 3569
RsrII CG'GwC_CG
Cuts at: 2124 2124
Size: 3929
SacI G_AGCT'C
Cuts at: 943 943
Size: 3929
SacII CC_GC'GG
Cuts at: 317 317
Size: 3929
SalI G'TCGA_C
Cuts at: 966 966
Size: 3929
SapI GCTCTTCn'nnn_
Cuts at: 751 1952 2162 751
Size: 1201 210 2518
Fragments arranged by size:
2518 1201 210
SbfI CC_TGCA'GG
Cuts at: 976 976
Size: 3929
ScaI AGT'ACT
Cuts at: 3446 3446
Size: 3929
SfcI C'TryA_G
Cuts at: 33 378 640 972 1657 2650 2841 33
Size: 345 262 332 685 993 191 1121
Fragments arranged by size:
1121 993 685 345 332 262 191
SfoI GGC'GCC
Cuts at: 1361 1609 1361
Size: 248 3681
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
SgrAI Cr'CCGG_yG
Cuts at: 763 763
Size: 3929
SmaI CCC'GGG
Cuts at: 137 228 951 137
Size: 91 723 3115
Fragments arranged by size:
3115 723 91
SmlI C'TyrA_G
Cuts at: 1084 1999 2491 2753 1084
Size: 915 492 262 2260
Fragments arranged by size:
2260 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3929
SphI G_CATG'C
Cuts at: 982 1482 982
Size: 500 3429
StyI C'CwwG_G
Cuts at: 107 1063 1417 107
Size: 956 354 2619
Fragments arranged by size:
2619 956 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1512 1645 2104 2313 1512
Size: 133 459 209 3128
Fragments arranged by size:
3128 459 209 133
TatI w'GTAC_w
Cuts at: 3444 3444
Size: 3929
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3446 3478 3446
Size: 32 3897
Tth111I GACn'n_nGTC
Cuts at: 1726 1726
Size: 3929
XbaI T'CTAG_A
Cuts at: 960 960
Size: 3929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1424 1424
Size: 3929
XmaI C'CCGG_G
Cuts at: 135 226 949 135
Size: 91 723 3115
Fragments arranged by size:
3115 723 91
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NotI NspI PciI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsrGI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NruI NsiI PacI
PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI