Restriction map for
pF1KB9419
(Circular) (Six-base) MAPSORT of: pF1KB9419.seq Check: 3627 from: 1 to: 4742
pF1KB9419 4742 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1780 1780
Size: 4742
Acc65I G'GTAC_C
Cuts at: 1758 1758
Size: 4742
AclI AA'CG_TT
Cuts at: 4582 4582
Size: 4742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 1148 1451 2567 2999 3746 4014 227
Size: 921 303 1116 432 747 268 955
Fragments arranged by size:
1116 955 921 747 432 303 268
AflII C'TTAA_G
Cuts at: 1186 1186
Size: 4742
AflIII A'CryG_T
Cuts at: 2094 3198 4175 2094
Size: 1104 977 2661
Fragments arranged by size:
2661 1104 977
AgeI A'CCGG_T
Cuts at: 3130 3130
Size: 4742
AhdI GACnn_n'nnGTC
Cuts at: 896 896
Size: 4742
AleI CACnn'nnGTG
Cuts at: 220 220
Size: 4742
AlwNI CAG_nnn'CTG
Cuts at: 178 1429 2047 3614 178
Size: 1251 618 1567 1306
Fragments arranged by size:
1567 1306 1251 618
ApaLI G'TGCA_C
Cuts at: 3512 3512
Size: 4742
ApoI r'AATT_y
Cuts at: 1153 1405 1484 1731 1746 4095 4351 1153
Size: 252 79 247 15 2349 256 1544
Fragments arranged by size:
2349 1544 256 252 247 79 15
AseI AT'TA_AT
Cuts at: 20 20
Size: 4742
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
AvaI C'yCGr_G
Cuts at: 534 926 1762 534
Size: 392 836 3514
Fragments arranged by size:
3514 836 392
BamHI G'GATC_C
Cuts at: 298 1767 298
Size: 1469 3273
BanI G'GyrC_C
Cuts at: 348 1758 2172 2420 2455 348
Size: 1410 414 248 35 2635
Fragments arranged by size:
2635 1410 414 248 35
BanII G_rGCy'C
Cuts at: 1756 2786 1756
Size: 1030 3712
BbeI G_GCGC'C
Cuts at: 2176 2424 2176
Size: 248 4494
BbsI GAAGACnn'nnnn_
Cuts at: 437 1379 1694 437
Size: 942 315 3485
Fragments arranged by size:
3485 942 315
BciVI GTATCCnnnnn_n'
Cuts at: 763 1564 2640 3401 4742 763
Size: 801 1076 761 1341 763
Fragments arranged by size:
1341 1076 801 763 761
BclI T'GATC_A
Cuts at: 2262 2262
Size: 4742
BglI GCCn_nnn'nGGC
Cuts at: 1592 1592
Size: 4742
BglII A'GATC_T
Cuts at: 1293 2257 4294 1293
Size: 964 2037 1741
Fragments arranged by size:
2037 1741 964
BlpI GC'TnA_GC
Cuts at: 1854 1854
Size: 4742
Bme1580I G_kGCm'C
Cuts at: 1588 2367 2460 3516 1588
Size: 779 93 1056 2814
Fragments arranged by size:
2814 1056 779 93
BmrI ACTGGGnnnn_n'
Cuts at: 1954 2035 2370 4514 1954
Size: 81 335 2144 2182
Fragments arranged by size:
2182 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3109 3109
Size: 4742
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 155
Size: 4742
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 878 1918 2797 3289 3587 878
Size: 1040 879 492 298 2033
Fragments arranged by size:
2033 1040 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4446 4446
Size: 4742
BsaAI yAC'GTr
Cuts at: 2095 2095
Size: 4742
BsaBI GATnn'nnATC
Cuts at: 6 2256 4293 6
Size: 2250 2037 455
Fragments arranged by size:
2250 2037 455
BsaHI Gr'CG_yC
Cuts at: 2173 2421 3123 4619 2173
Size: 248 702 1496 2296
Fragments arranged by size:
2296 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1931 2452 3130 3404 3551 3962 1931
Size: 521 678 274 147 411 2711
Fragments arranged by size:
2711 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2167 2925 3502 3998 2167
Size: 758 577 496 2911
Fragments arranged by size:
2911 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1421 1421
Size: 4742
BsiEI CG_ry'CG
Cuts at: 104 2284 2330 3538 104
Size: 2180 46 1208 1308
Fragments arranged by size:
2180 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 792 1756 2534 2724 3516 792
Size: 964 778 190 792 2018
Fragments arranged by size:
2018 964 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 792 1588 1756 2367 2460 2534 2724 2786 3516
Size: 796 168 611 93 74 190 62 730
Cuts at: 3516 792
Size: 2018
Fragments arranged by size:
2018 796 730 611 190 168 93 74
62
BspEI T'CCGG_A
Cuts at: 3962 3962
Size: 4742
BspHI T'CATG_A
Cuts at: 3918 3918
Size: 4742
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1792 2006 2308 2689 1792
Size: 214 302 381 3845
Fragments arranged by size:
3845 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3034 3088 4572 4737 50
Size: 2984 54 1484 165 55
Fragments arranged by size:
2984 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2654 2654
Size: 4742
BsrFI r'CCGG_y
Cuts at: 2740 2921 3130 2740
Size: 181 209 4352
Fragments arranged by size:
4352 209 181
BssSI C'ACGA_G
Cuts at: 3013 3371 3013
Size: 358 4384
BstAPI GCAn_nnn'nTGC
Cuts at: 910 2119 910
Size: 1209 3533
BstBI TT'CG_AA
Cuts at: 3103 3103
Size: 4742
BstXI CCAn_nnnn'nTGG
Cuts at: 1418 1418
Size: 4742
BstYI r'GATC_y
Cuts at: 1 298 1293 1767 2234 2257 2592 2838 3850
Size: 297 995 474 467 23 335 246 1012
Cuts at: 3850 3936 3948 4294 1
Size: 86 12 346 449
Fragments arranged by size:
1012 995 474 467 449 346 335 297
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 303 1589 303
Size: 1286 3456
BtgI C'CryG_G
Cuts at: 107 107
Size: 4742
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2692 2873 3036 2692
Size: 181 163 4398
Fragments arranged by size:
4398 181 163
BtsI GCAGTG_nn'
Cuts at: 1697 1985 1697
Size: 288 4454
DraI TTT'AAA
Cuts at: 1741 1741
Size: 4742
DrdI GACnn_nn'nnGTC
Cuts at: 2448 3306 2448
Size: 858 3884
EaeI y'GGCC_r
Cuts at: 2327 2501 2892 2919 4264 2327
Size: 174 391 27 1345 2805
Fragments arranged by size:
2805 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2327 2327
Size: 4742
EarI CTCTTCn'nnn_
Cuts at: 2765 2975 2765
Size: 210 4532
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2950 3260 3406 4552 2950
Size: 310 146 1146 3140
Fragments arranged by size:
3140 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1754 1754
Size: 4742
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 155 227 1148 1451 2567 2999 3746 4014 155
Size: 72 921 303 1116 432 747 268 883
Fragments arranged by size:
1116 921 883 747 432 303 268 72
EcoNI CCTnn'n_nnAGG
Cuts at: 320 320
Size: 4742
EcoO109I rG'GnC_Cy
Cuts at: 1641 1881 1641
Size: 240 4502
EcoRI G'AATT_C
Cuts at: 1484 1746 1484
Size: 262 4480
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1075 2761 1075
Size: 1686 3056
FspI TGC'GCA
Cuts at: 2523 2523
Size: 4742
HaeII r_GCGC'y
Cuts at: 553 2176 2424 3446 4425 553
Size: 1623 248 1022 979 870
Fragments arranged by size:
1623 1022 979 870 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 761 793 882 914 1459 1491 2584 2614 2616
Size: 32 89 32 545 32 1093 30 2
Cuts at: 2616 2646 761
Size: 30 2857
Fragments arranged by size:
2857 1093 545 89 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 860 1781 860
Size: 921 3821
HindIII A'AGCT_T
Cuts at: 1052 1170 1213 1052
Size: 118 43 4581
Fragments arranged by size:
4581 118 43
KasI G'GCGC_C
Cuts at: 2172 2420 2172
Size: 248 4494
KpnI G_GTAC'C
Cuts at: 1762 1762
Size: 4742
MluI A'CGCG_T
Cuts at: 4175 4175
Size: 4742
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 898 1612 3413 3597 898
Size: 714 1801 184 2043
Fragments arranged by size:
2043 1801 714 184
MscI TGG'CCA
Cuts at: 2503 2503
Size: 4742
MslI CAynn'nnrTG
Cuts at: 220 839 2731 2858 220
Size: 619 1892 127 2104
Fragments arranged by size:
2104 1892 619 127
MspA1I CmG'CkG
Cuts at: 1224 1853 2167 2527 3540 3785 1224
Size: 629 314 360 1013 245 2181
Fragments arranged by size:
2181 1013 629 360 314 245
NaeI GCC'GGC
Cuts at: 2923 2923
Size: 4742
NarI GG'CG_CC
Cuts at: 2173 2421 2173
Size: 248 4494
NcoI C'CATG_G
Cuts at: 107 107
Size: 4742
NgoMIV G'CCGG_C
Cuts at: 2921 2921
Size: 4742
NspI r_CATG'y
Cuts at: 838 1795 2295 3202 838
Size: 957 500 907 2378
Fragments arranged by size:
2378 957 907 500
PciI A'CATG_T
Cuts at: 3198 3198
Size: 4742
PfoI T'CCnGG_A
Cuts at: 630 630
Size: 4742
PmeI GTTT'AAAC
Cuts at: 1741 1741
Size: 4742
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3881 3913 3881
Size: 32 4710
PpuMI rG'GwC_Cy
Cuts at: 1641 1641
Size: 4742
PstI C_TGCA'G
Cuts at: 558 809 1789 2474 558
Size: 251 980 685 2826
Fragments arranged by size:
2826 980 685 251
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4742
PvuII CAG'CTG
Cuts at: 1224 2167 2527 1224
Size: 943 360 3439
Fragments arranged by size:
3439 943 360
RsrII CG'GwC_CG
Cuts at: 2937 2937
Size: 4742
SacI G_AGCT'C
Cuts at: 1756 1756
Size: 4742
SalI G'TCGA_C
Cuts at: 1779 1779
Size: 4742
SapI GCTCTTCn'nnn_
Cuts at: 2765 2975 2765
Size: 210 4532
SbfI CC_TGCA'GG
Cuts at: 1789 1789
Size: 4742
ScaI AGT'ACT
Cuts at: 1550 4259 1550
Size: 2709 2033
SexAI A'CCwGG_T
Cuts at: 1032 1032
Size: 4742
SfcI C'TryA_G
Cuts at: 33 218 425 512 554 805 1703 1785 2470
Size: 185 207 87 42 251 898 82 685
Cuts at: 2470 3463 3654 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 898 685 251 207 191 185
87 82 42
SfoI GGC'GCC
Cuts at: 2174 2422 2174
Size: 248 4494
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4742
SmaI CCC'GGG
Cuts at: 1764 1764
Size: 4742
SmlI C'TyrA_G
Cuts at: 893 926 1186 1897 2812 3304 3566 893
Size: 33 260 711 915 492 262 2069
Fragments arranged by size:
2069 915 711 492 262 260 33
SpeI A'CTAG_T
Cuts at: 67 1500 67
Size: 1433 3309
SphI G_CATG'C
Cuts at: 1795 2295 1795
Size: 500 4242
SspI AAT'ATT
Cuts at: 1311 1311
Size: 4742
StyI C'CwwG_G
Cuts at: 107 1876 2230 107
Size: 1769 354 2619
Fragments arranged by size:
2619 1769 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2325 2458 2917 3126 2325
Size: 133 459 209 3941
Fragments arranged by size:
3941 459 209 133
TatI w'GTAC_w
Cuts at: 184 1548 4257 184
Size: 1364 2709 669
Fragments arranged by size:
2709 1364 669
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4259 4291 4259
Size: 32 4710
Tth111I GACn'n_nGTC
Cuts at: 2539 2539
Size: 4742
XbaI T'CTAG_A
Cuts at: 1773 1773
Size: 4742
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2237 2237
Size: 4742
XhoI C'TCGA_G
Cuts at: 926 926
Size: 4742
XmaI C'CCGG_G
Cuts at: 1762 1762
Size: 4742
XmnI GAAnn'nnTTC
Cuts at: 458 1670 458
Size: 1212 3530
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsaXI
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI StuI SwaI ZraI