Restriction map for
pF1KB9418
(Circular) (Six-base) MAPSORT of: pF1KB9418.seq Check: 8352 from: 1 to: 4970
pF1KB9418 4970 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 561 561
Size: 4970
AccI GT'mk_AC
Cuts at: 2008 2008
Size: 4970
Acc65I G'GTAC_C
Cuts at: 1986 1986
Size: 4970
AclI AA'CG_TT
Cuts at: 4810 4810
Size: 4970
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 512 572 1022 1674 1754 1850 2795 3227
Size: 177 60 450 652 80 96 945 432
Cuts at: 3227 3974 4242 335
Size: 747 268 1063
Fragments arranged by size:
1063 945 747 652 450 432 268 177
96 80 60
AfeI AGC'GCT
Cuts at: 958 958
Size: 4970
AflIII A'CryG_T
Cuts at: 219 2322 3426 4403 219
Size: 2103 1104 977 786
Fragments arranged by size:
2103 1104 977 786
AgeI A'CCGG_T
Cuts at: 3358 3358
Size: 4970
AleI CACnn'nnGTG
Cuts at: 523 523
Size: 4970
AlwNI CAG_nnn'CTG
Cuts at: 1489 2275 3842 1489
Size: 786 1567 2617
Fragments arranged by size:
2617 1567 786
ApaI G_GGCC'C
Cuts at: 1609 1609
Size: 4970
ApaLI G'TGCA_C
Cuts at: 3740 3740
Size: 4970
ApoI r'AATT_y
Cuts at: 145 1152 1341 1974 4323 4579 145
Size: 1007 189 633 2349 256 536
Fragments arranged by size:
2349 1007 633 536 256 189
AseI AT'TA_AT
Cuts at: 20 20
Size: 4970
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4970
AvaI C'yCGr_G
Cuts at: 1990 1990
Size: 4970
AvrII C'CTAG_G
Cuts at: 1405 1405
Size: 4970
BamHI G'GATC_C
Cuts at: 1014 1995 1014
Size: 981 3989
BanI G'GyrC_C
Cuts at: 1986 2400 2648 2683 1986
Size: 414 248 35 4273
Fragments arranged by size:
4273 414 248 35
BanII G_rGCy'C
Cuts at: 278 1585 1609 1984 3014 278
Size: 1307 24 375 1030 2234
Fragments arranged by size:
2234 1307 1030 375 24
BbeI G_GCGC'C
Cuts at: 2404 2652 2404
Size: 248 4722
BbsI GAAGACnn'nnnn_
Cuts at: 1028 1028
Size: 4970
BbvCI CC'TCA_GC
Cuts at: 413 413
Size: 4970
BciVI GTATCCnnnnn_n'
Cuts at: 2868 3629 4970 2868
Size: 761 1341 2868
Fragments arranged by size:
2868 1341 761
BclI T'GATC_A
Cuts at: 2490 2490
Size: 4970
BglII A'GATC_T
Cuts at: 990 2485 4522 990
Size: 1495 2037 1438
Fragments arranged by size:
2037 1495 1438
BlpI GC'TnA_GC
Cuts at: 2082 2082
Size: 4970
Bme1580I G_kGCm'C
Cuts at: 1609 2595 2688 3744 1609
Size: 986 93 1056 2835
Fragments arranged by size:
2835 1056 986 93
BmrI ACTGGGnnnn_n'
Cuts at: 508 2182 2263 2598 4742 508
Size: 1674 81 335 2144 736
Fragments arranged by size:
2144 1674 736 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3337 3337
Size: 4970
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 745
Size: 4970
Bpu10I CC'TnA_GC
Cuts at: 413 413
Size: 4970
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1769 2146 3025 3517 3815 1769
Size: 377 879 492 298 2924
Fragments arranged by size:
2924 879 492 377 298
BsaI GGTCTCn'nnnn_
Cuts at: 886 4674 886
Size: 3788 1182
BsaAI yAC'GTr
Cuts at: 2323 2323
Size: 4970
BsaBI GATnn'nnATC
Cuts at: 6 1013 2484 4521 6
Size: 1007 1471 2037 455
Fragments arranged by size:
2037 1471 1007 455
BsaHI Gr'CG_yC
Cuts at: 2401 2649 3351 4847 2401
Size: 248 702 1496 2524
Fragments arranged by size:
2524 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1256 2159 2680 3358 3632 3779 4190 1256
Size: 903 521 678 274 147 411 2036
Fragments arranged by size:
2036 903 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1218 1248 1704 1734 1218
Size: 30 456 30 4454
Fragments arranged by size:
4454 456 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 412 1139 1577 1712 412
Size: 727 438 135 3670
Fragments arranged by size:
3670 727 438 135
BseYI C'CCAG_C
Cuts at: 669 800 1247 2395 3153 3730 4226 669
Size: 131 447 1148 758 577 496 1413
Fragments arranged by size:
1413 1148 758 577 496 447 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 1685 1240
Size: 445 4525
BsiEI CG_ry'CG
Cuts at: 104 1093 2512 2558 3766 104
Size: 989 1419 46 1208 1308
Fragments arranged by size:
1419 1308 1208 989 46
BsiHKAI G_wGCw'C
Cuts at: 909 1984 2762 2952 3744 909
Size: 1075 778 190 792 2135
Fragments arranged by size:
2135 1075 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 278 909 1585 1609 1984 2595 2688 2762 2952
Size: 631 676 24 375 611 93 74 190
Cuts at: 2952 3014 3744 278
Size: 62 730 1504
Fragments arranged by size:
1504 730 676 631 611 375 190 93
74 62 24
BspEI T'CCGG_A
Cuts at: 1256 4190 1256
Size: 2934 2036
BspHI T'CATG_A
Cuts at: 375 4146 375
Size: 3771 1199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 561 1474 1614 2020 2234 2536 2917 561
Size: 913 140 406 214 302 381 2614
Fragments arranged by size:
2614 913 406 381 302 214 140
BsrBI CCG'CTC
Cuts at: 50 3262 3316 4800 4965 50
Size: 3212 54 1484 165 55
Fragments arranged by size:
3212 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1644 1662 2882 1644
Size: 18 1220 3732
Fragments arranged by size:
3732 1220 18
BsrFI r'CCGG_y
Cuts at: 253 1088 2968 3149 3358 253
Size: 835 1880 181 209 1865
Fragments arranged by size:
1880 1865 835 209 181
BssSI C'ACGA_G
Cuts at: 3241 3599 3241
Size: 358 4612
BstAPI GCAn_nnn'nTGC
Cuts at: 2347 2347
Size: 4970
BstBI TT'CG_AA
Cuts at: 3331 3331
Size: 4970
BstEII G'GTnAC_C
Cuts at: 1710 1710
Size: 4970
BstYI r'GATC_y
Cuts at: 1 990 1014 1041 1176 1473 1492 1995 2462
Size: 989 24 27 135 297 19 503 467
Cuts at: 2462 2485 2820 3066 4078 4164 4176 4522 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 989 503 467 449 346 335 297
246 135 86 27 24 23 19 12
BtgI C'CryG_G
Cuts at: 107 896 107
Size: 789 4181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2920 3101 3264 2920
Size: 181 163 4626
Fragments arranged by size:
4626 181 163
BtsI GCAGTG_nn'
Cuts at: 754 848 2213 754
Size: 94 1365 3511
Fragments arranged by size:
3511 1365 94
DraI TTT'AAA
Cuts at: 822 975 1969 822
Size: 153 994 3823
Fragments arranged by size:
3823 994 153
DrdI GACnn_nn'nnGTC
Cuts at: 2676 3534 2676
Size: 858 4112
EaeI y'GGCC_r
Cuts at: 2555 2729 3120 3147 4492 2555
Size: 174 391 27 1345 3033
Fragments arranged by size:
3033 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2555 2555
Size: 4970
EarI CTCTTCn'nnn_
Cuts at: 266 897 1258 2993 3203 266
Size: 631 361 1735 210 2033
Fragments arranged by size:
2033 1735 631 361 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3178 3488 3634 4780 3178
Size: 310 146 1146 3368
Fragments arranged by size:
3368 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1982 1982
Size: 4970
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 512 572 745 1022 1674 1754 1850 2795
Size: 177 60 173 277 652 80 96 945
Cuts at: 2795 3227 3974 4242 335
Size: 432 747 268 1063
Fragments arranged by size:
1063 945 747 652 432 277 268 177
173 96 80 60
EcoO109I rG'GnC_Cy
Cuts at: 1606 1626 2109 1606
Size: 20 483 4467
Fragments arranged by size:
4467 483 20
EcoRI G'AATT_C
Cuts at: 1152 1974 1152
Size: 822 4148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2989 2989
Size: 4970
FspI TGC'GCA
Cuts at: 2751 2751
Size: 4970
HaeII r_GCGC'y
Cuts at: 960 2404 2652 3674 4653 960
Size: 1444 248 1022 979 1277
Fragments arranged by size:
1444 1277 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1704 1736 2812 2842 2844 2874 1704
Size: 32 1076 30 2 30 3800
Fragments arranged by size:
3800 1076 32 30 30 2
HincII GTy'rAC
Cuts at: 2009 2009
Size: 4970
HindIII A'AGCT_T
Cuts at: 365 365
Size: 4970
KasI G'GCGC_C
Cuts at: 2400 2648 2400
Size: 248 4722
KpnI G_GTAC'C
Cuts at: 1990 1990
Size: 4970
MfeI C'AATT_G
Cuts at: 392 1595 392
Size: 1203 3767
MluI A'CGCG_T
Cuts at: 4403 4403
Size: 4970
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 147 1402 3641 3825 147
Size: 1255 2239 184 1292
Fragments arranged by size:
2239 1292 1255 184
MscI TGG'CCA
Cuts at: 2731 2731
Size: 4970
MslI CAynn'nnrTG
Cuts at: 523 2959 3086 523
Size: 2436 127 2407
Fragments arranged by size:
2436 2407 127
MspA1I CmG'CkG
Cuts at: 1737 2081 2395 2755 3768 4013 1737
Size: 344 314 360 1013 245 2694
Fragments arranged by size:
2694 1013 360 344 314 245
NaeI GCC'GGC
Cuts at: 3151 3151
Size: 4970
NarI GG'CG_CC
Cuts at: 2401 2649 2401
Size: 248 4722
NcoI C'CATG_G
Cuts at: 107 896 107
Size: 789 4181
NgoMIV G'CCGG_C
Cuts at: 3149 3149
Size: 4970
NspI r_CATG'y
Cuts at: 223 2023 2523 3430 223
Size: 1800 500 907 1763
Fragments arranged by size:
1800 1763 907 500
PciI A'CATG_T
Cuts at: 219 3426 219
Size: 3207 1763
PflMI CCAn_nnn'nTGG
Cuts at: 854 854
Size: 4970
PfoI T'CCnGG_A
Cuts at: 761 761
Size: 4970
PmeI GTTT'AAAC
Cuts at: 1969 1969
Size: 4970
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 4109
Size: 32 4938
PpuMI rG'GwC_Cy
Cuts at: 1626 1626
Size: 4970
PspOMI G'GGCC_C
Cuts at: 1605 1605
Size: 4970
PstI C_TGCA'G
Cuts at: 1568 2017 2702 1568
Size: 449 685 3836
Fragments arranged by size:
3836 685 449
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4970
PvuII CAG'CTG
Cuts at: 1737 2395 2755 1737
Size: 658 360 3952
Fragments arranged by size:
3952 658 360
RsrII CG'GwC_CG
Cuts at: 3165 3165
Size: 4970
SacI G_AGCT'C
Cuts at: 1984 1984
Size: 4970
SalI G'TCGA_C
Cuts at: 2007 2007
Size: 4970
SapI GCTCTTCn'nnn_
Cuts at: 266 897 1258 2993 3203 266
Size: 631 361 1735 210 2033
Fragments arranged by size:
2033 1735 631 361 210
SbfI CC_TGCA'GG
Cuts at: 2017 2017
Size: 4970
ScaI AGT'ACT
Cuts at: 1544 4487 1544
Size: 2943 2027
SfcI C'TryA_G
Cuts at: 33 1133 1564 2013 2698 3691 3882 33
Size: 1100 431 449 685 993 191 1121
Fragments arranged by size:
1121 1100 993 685 449 431 191
SfoI GGC'GCC
Cuts at: 2402 2650 2402
Size: 248 4722
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4970
SmaI CCC'GGG
Cuts at: 1992 1992
Size: 4970
SmlI C'TyrA_G
Cuts at: 1784 2125 3040 3532 3794 1784
Size: 341 915 492 262 2960
Fragments arranged by size:
2960 915 492 341 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4970
SphI G_CATG'C
Cuts at: 2023 2523 2023
Size: 500 4470
StyI C'CwwG_G
Cuts at: 107 896 1405 2104 2458 107
Size: 789 509 699 354 2619
Fragments arranged by size:
2619 789 699 509 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 535 1763 2553 2686 3145 3354 535
Size: 1228 790 133 459 209 2151
Fragments arranged by size:
2151 1228 790 459 209 133
TatI w'GTAC_w
Cuts at: 1272 1281 1542 4485 1272
Size: 9 261 2943 1757
Fragments arranged by size:
2943 1757 261 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1218 1250 4487 4519 1218
Size: 32 3237 32 1669
Fragments arranged by size:
3237 1669 32 32
Tth111I GACn'n_nGTC
Cuts at: 2767 2767
Size: 4970
XbaI T'CTAG_A
Cuts at: 2001 2001
Size: 4970
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1179 2465 1179
Size: 1286 3684
XmaI C'CCGG_G
Cuts at: 1990 1990
Size: 4970
XmnI GAAnn'nnTTC
Cuts at: 221 221
Size: 4970
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI BaeI BcgI
BfrBI BglI BmgBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI NdeI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI